By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from...By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from the skin of hybrid sturgeon with two extraction methods. The yields of ASC and PSC based on the wet weight of skin were 5.73 ± 0.11% and 10.26 ± 0.39%, respectively. The denaturation and melting points of ASC(26.83 ℃ and 110.49 ℃) and PSC(26.54 ℃ and 102.99 ℃) were assessed by Circular dichroism(CD) and Differential scanning calorimetry(DSC). ASC and PSC appeared to be dense sheet-like film linked by random-coiled filaments under scanning electron microscopy(SEM). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Fourier transform infrared spectroscopy(FTIR) confirmed that both the ASC and PSC were Type I collagen and maintained a complete triple helix structure. These results indicated that both ASC and PSC possessed good biological activity and could be widely used in medical biomaterials and other fields.展开更多
The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrat...The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg^-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg^-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality.展开更多
Non-metallic inclusions are a significant factor causing fractures during the manufacturing process of tire cord steel,and dissolution in the steel profoundly affects them.How the basicity and Al_(2)O_(3) content in S...Non-metallic inclusions are a significant factor causing fractures during the manufacturing process of tire cord steel,and dissolution in the steel profoundly affects them.How the basicity and Al_(2)O_(3) content in SiO_(2)-CaO-Al_(2)O_(3)-MgO refining slag affect non-metallic inclusions in tire cord steel at 1873 K was investigated.A quantitative relationship has been established between the slag basicity and the dissolved oxygen content in steel.The results demonstrate that non-metallic inclusions in steel transform along SiO_(2)-MnO-Al_(2)O_(3)→SiO_(2)-MnO system of inclusions.When the basicity is controlled within the range of 0.8-1.0,the corresponding dissolved oxygen content should be between 4×10^(−6) and 10×10^(−6).When Al_(2)O_(3) content in the refining slag is maintained at 5%,and the slag basicity is controlled between 0.8 and 1.0,or if the slag basicity is precisely 1.0 with Al_(2)O_(3) content kept below 11%,control over the plasticization of SiO_(2)-MnO-Al_(2)O_(3) system within the inclusions can be effectively achieved.Combined with thermodynamic calculation and measurement of the dissolved oxygen([O])activity in the steel,controlling SiO_(2)-MnO-Al_(2)O_(3)-like inclusions in the plastic region range can be achieved by adjusting the refining slag composition.展开更多
基金Funded by the National Natural Science Foundation of China(No.51173143)
文摘By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from the skin of hybrid sturgeon with two extraction methods. The yields of ASC and PSC based on the wet weight of skin were 5.73 ± 0.11% and 10.26 ± 0.39%, respectively. The denaturation and melting points of ASC(26.83 ℃ and 110.49 ℃) and PSC(26.54 ℃ and 102.99 ℃) were assessed by Circular dichroism(CD) and Differential scanning calorimetry(DSC). ASC and PSC appeared to be dense sheet-like film linked by random-coiled filaments under scanning electron microscopy(SEM). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Fourier transform infrared spectroscopy(FTIR) confirmed that both the ASC and PSC were Type I collagen and maintained a complete triple helix structure. These results indicated that both ASC and PSC possessed good biological activity and could be widely used in medical biomaterials and other fields.
基金supported by the Major Special Science and Technology Projects in Shandong Province (No. 2015 ZDZX05003)the Fundamental Research Funds for the Central Universities (Nos. 201564018, 201513048)
文摘The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg^-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg^-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality.
基金supported by the National Natural Science Foundation of China(Nos.52274337,52174317 and U1960203)2023 Liaoning Province Department of Education Fundamental Research Project Achievements(No.JYTMS20230928).
文摘Non-metallic inclusions are a significant factor causing fractures during the manufacturing process of tire cord steel,and dissolution in the steel profoundly affects them.How the basicity and Al_(2)O_(3) content in SiO_(2)-CaO-Al_(2)O_(3)-MgO refining slag affect non-metallic inclusions in tire cord steel at 1873 K was investigated.A quantitative relationship has been established between the slag basicity and the dissolved oxygen content in steel.The results demonstrate that non-metallic inclusions in steel transform along SiO_(2)-MnO-Al_(2)O_(3)→SiO_(2)-MnO system of inclusions.When the basicity is controlled within the range of 0.8-1.0,the corresponding dissolved oxygen content should be between 4×10^(−6) and 10×10^(−6).When Al_(2)O_(3) content in the refining slag is maintained at 5%,and the slag basicity is controlled between 0.8 and 1.0,or if the slag basicity is precisely 1.0 with Al_(2)O_(3) content kept below 11%,control over the plasticization of SiO_(2)-MnO-Al_(2)O_(3) system within the inclusions can be effectively achieved.Combined with thermodynamic calculation and measurement of the dissolved oxygen([O])activity in the steel,controlling SiO_(2)-MnO-Al_(2)O_(3)-like inclusions in the plastic region range can be achieved by adjusting the refining slag composition.
