The recent development of the two-photon technique offers a unique opportunity to improve the depth of imaging and treatment in photodynamic therapy(PDT).Herein,we designed a fluorescence resonance energy transfer(FRE...The recent development of the two-photon technique offers a unique opportunity to improve the depth of imaging and treatment in photodynamic therapy(PDT).Herein,we designed a fluorescence resonance energy transfer(FRET)based AceDAN-porphyrin(Zn)dyad for two-photon excited fluorescence imaging and PDT of cancer cells simultaneously,in which the AceDAN moiety was selected as the two-photon absorption donor and the porphyrin(Zn)moiety served as the energy acceptor.Upon one-photon or two-photon excitation,the excited state energy of the AceDAN donor was transferred to the porphyrin(Zn)acceptor with high efficiency(η_(EET)=98%),where red fluorescence and singlet oxygen were generated.Dyad 1 exhibited high photocytotoxicity towards A549 cells(IC_(50)=4.3μM)with rapid cellular uptake while displaying low dark-cytotoxicity.Furthermore,by combining the advantages of two-photon excitation with a porphyrin(Zn)photosensitizer,the AceDAN-porphyrin(Zn)dyad has been successfully applied to A549 cells for imaging and photodynamic therapy under two-photon laser irradiation.展开更多
基金National Natural Science Foundation of China(21471015 and 21631003)Beijing Natural Science FoundationFundamental Research Funds for the Central Universities。
文摘The recent development of the two-photon technique offers a unique opportunity to improve the depth of imaging and treatment in photodynamic therapy(PDT).Herein,we designed a fluorescence resonance energy transfer(FRET)based AceDAN-porphyrin(Zn)dyad for two-photon excited fluorescence imaging and PDT of cancer cells simultaneously,in which the AceDAN moiety was selected as the two-photon absorption donor and the porphyrin(Zn)moiety served as the energy acceptor.Upon one-photon or two-photon excitation,the excited state energy of the AceDAN donor was transferred to the porphyrin(Zn)acceptor with high efficiency(η_(EET)=98%),where red fluorescence and singlet oxygen were generated.Dyad 1 exhibited high photocytotoxicity towards A549 cells(IC_(50)=4.3μM)with rapid cellular uptake while displaying low dark-cytotoxicity.Furthermore,by combining the advantages of two-photon excitation with a porphyrin(Zn)photosensitizer,the AceDAN-porphyrin(Zn)dyad has been successfully applied to A549 cells for imaging and photodynamic therapy under two-photon laser irradiation.
