This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragment...This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in E.coli hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed.展开更多
Skin sensitization is a common adverse effect of a wide range of small reactive chemicals,leading to allergic contact dermatitis(ACD),the most frequent manifestation of immunotoxicity in humans.The prevalence of ACD i...Skin sensitization is a common adverse effect of a wide range of small reactive chemicals,leading to allergic contact dermatitis(ACD),the most frequent manifestation of immunotoxicity in humans.The prevalence of ACD is increasing,affecting up to 20%of the Western European population.This trend was particularly pronounced in high-risk occupational sectors,including healthcare,food services,metal and construction workers,and hairdressers[1].The skin sensitization adverse outcome pathway(AOP)comprises 11 elements,with four designated key events(KEs):formation of proteinhapten complexes(KE-1),inflammatory keratinocyte response(KE-2),dendritic cell(DC)activation(KE-3),and T-cell proliferation(KE-4)[2].As there is no cure for ACD,preventive strategies are of great relevance.In addition to avoiding exposure,preventive measures,such as the use of latex gloves,barrier creams,emollients,and moisturizers,often have limited effectiveness[3].展开更多
Considering that actual systems are often constrained by multiple factors such as state limitation,actuator saturation and actuator failure at the same time,this paper provides an effective solution for non-affine mul...Considering that actual systems are often constrained by multiple factors such as state limitation,actuator saturation and actuator failure at the same time,this paper provides an effective solution for non-affine multi-player systems,which can guarantee the required performance while saving communication cost.Initially,an auxiliary system is established to accommodate state limitations,following which the controller design is partitioned into two distinct segments,addressing different types of faults.Specifically,the discontinuous and continuous aspects of the controller are achieved by sliding-mode control(SMC)and adaptive critic design(ACD),respectively.During the implementation of ACD to solve the guaranteed value function incorporating the utility function designed for the asymmetric saturation of the control input,two adaptive schemes including adaptive eventtriggered impulsive control(AETIC)and adaptive self-triggered impulsive control(ASTIC)are introduced successively.It is proved that the system maintains exponential stability rather than asymptotic stability and the state signals keep ultimately uniformly bounded(UUB).Finally,the effectiveness of the proposed control sequence is verified by simulation comparisons.展开更多
High-frequency data analysis is a new research field in financial econometrics. This paper reviews main results about empirical market and econometric modeling of high-frequency data, and argues about its prospect and...High-frequency data analysis is a new research field in financial econometrics. This paper reviews main results about empirical market and econometric modeling of high-frequency data, and argues about its prospect and importance in Chinese financial market.展开更多
文摘This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in E.coli hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed.
基金support was provided by the European Regional Development Fund(ERDF),through the Centro 2020 Regional Operational Programme,Portugal(Project No.:CENTRO-01-0145-FEDER-000012(HealthyAging2020))through the COMPETE 2020-Operational Programme for Competitiveness and Internationalisation and Portuguese National Funds via Fundaçao para a Ciencia e a Tecnologia,Portugal(Project Nos.:POCI-01-0145-FEDER-029369 UIDB/04539/2020,iBiMED UIDB/04501/2020,DOI identifier https://doi.org/10.54499/UIDB/04501/2020 and project reference UIDP/04501/2020,DOI identifier https://doi.org/10.54499/UIDP/04501/2020,and LA/P/0058/2020)supported by Fundaçao para a Ciencia e a Tecnologia through the individual PhD fellowships,Portugal(Grant Nos.:PD/BDE/142926/2018 and SFRH/BD/110717/2015)。
文摘Skin sensitization is a common adverse effect of a wide range of small reactive chemicals,leading to allergic contact dermatitis(ACD),the most frequent manifestation of immunotoxicity in humans.The prevalence of ACD is increasing,affecting up to 20%of the Western European population.This trend was particularly pronounced in high-risk occupational sectors,including healthcare,food services,metal and construction workers,and hairdressers[1].The skin sensitization adverse outcome pathway(AOP)comprises 11 elements,with four designated key events(KEs):formation of proteinhapten complexes(KE-1),inflammatory keratinocyte response(KE-2),dendritic cell(DC)activation(KE-3),and T-cell proliferation(KE-4)[2].As there is no cure for ACD,preventive strategies are of great relevance.In addition to avoiding exposure,preventive measures,such as the use of latex gloves,barrier creams,emollients,and moisturizers,often have limited effectiveness[3].
基金supported in part by the National Natural Science Foundation of China(62273036,62403045)the Open Research Fund of the State Key Laboratory of Multimodal Artificial Intelligence Systems(MAIS2025020)Interdisciplinary Research Project for Young Teachers of USTB(Fundamental Research Funds for the Central Universities)(FRF-IDRY-23-036).
文摘Considering that actual systems are often constrained by multiple factors such as state limitation,actuator saturation and actuator failure at the same time,this paper provides an effective solution for non-affine multi-player systems,which can guarantee the required performance while saving communication cost.Initially,an auxiliary system is established to accommodate state limitations,following which the controller design is partitioned into two distinct segments,addressing different types of faults.Specifically,the discontinuous and continuous aspects of the controller are achieved by sliding-mode control(SMC)and adaptive critic design(ACD),respectively.During the implementation of ACD to solve the guaranteed value function incorporating the utility function designed for the asymmetric saturation of the control input,two adaptive schemes including adaptive eventtriggered impulsive control(AETIC)and adaptive self-triggered impulsive control(ASTIC)are introduced successively.It is proved that the system maintains exponential stability rather than asymptotic stability and the state signals keep ultimately uniformly bounded(UUB).Finally,the effectiveness of the proposed control sequence is verified by simulation comparisons.
文摘High-frequency data analysis is a new research field in financial econometrics. This paper reviews main results about empirical market and econometric modeling of high-frequency data, and argues about its prospect and importance in Chinese financial market.
