[目的/意义]展现一个学科与其他学科之间知识吸收和知识扩散的完整过程,揭示学科知识发展的来龙去脉。[方法/过程]利用国际权威引文数据库Web of Science,依据Web of Science学科类别划分标准,以Information Science&Library Scien...[目的/意义]展现一个学科与其他学科之间知识吸收和知识扩散的完整过程,揭示学科知识发展的来龙去脉。[方法/过程]利用国际权威引文数据库Web of Science,依据Web of Science学科类别划分标准,以Information Science&Library Science(以下简称ISLS)为例,选择2006-2010年和2011-2015年两个时间段,建立三个数据集(施引文献集、原始文献集、被引文献集),利用双图叠加可视化的方式探析ISLS与其他学科在期刊层面的知识吸收过程;利用知识扩散广度、强度和速度三个指标揭示ISLS与其他学科之间知识扩散现状和发展趋势。[结果/结论]在两个阶段内,ISLS主要吸收期刊类群心理、教育、社会的知识,知识来源学科主要为计算机科学学和管理学,在相应时间段内,知识扩散广度与时间呈对数函数关系,知识扩散速度与时间呈指数函数关系,ISLS向其他学科的知识扩散强度呈幂率分布。展开更多
MoS_(2)nanosheets(NSs)are novel 2D nanomaterials(NMs)with potential uses in many areas,and therefore oral exposure route to MoS_(2)NSs is plausible.Currently,MoS_(2)NSs are considered as biocompatible NMs,but there is...MoS_(2)nanosheets(NSs)are novel 2D nanomaterials(NMs)with potential uses in many areas,and therefore oral exposure route to MoS_(2)NSs is plausible.Currently,MoS_(2)NSs are considered as biocompatible NMs,but there is lacking of systemic investigations to study the interactions of MoS_(2)NSs with intestinal cells.In this study,we exposed the 3D Caco-2 spheroids to MoS_(2)NSs or MoS_(2)powders(denoted as MoS_(2)-bulk),and investigated the potential adverse effects of MoS_(2)-materials based on transcriptomics and lipidomics analysis.As expected,both MoS_(2)NSs and MoS_(2)-bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity,membrane disruption or decrease of thiols.However,the Gene Ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analysis indicated that nutrient absorption and metabolism was decreased.One of the most significantly decreased KEGG pathways is fat digestion and absorption(map04975),and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein A1,key proteins involved in fat digestion and absorption,were down-regulated by MoS_(2)NSs or MoS_(2)-bulk.In addition,BODIPY 493/503 staining suggested that exposure to MoS_(2)NSs and MoS_(2)-bulk decreased lipid levels in the spheroids.However,lipidomics data indicated that MoS_(2)materials only decreased 8 lipid classes,including lysophosphatidylcholine,lysodimethylphosphatidylethanolamine,N-acylethanolamine,ceramide phosphoethanolamines,gangliosides,lysosphingomyelin and sulfatide,whereas most of the lipid classes were indeed increased.In addition,MoS_(2)NSs was more potent to decrease the lipid classes compared with MoS_(2)-bulk.Combined,the results from this study showed that MoS_(2)NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids.展开更多
采用改进的布里奇曼法生长出Cd Si P_2单晶体,运用X射线能谱仪、傅里叶变换红外分光光度计以及红外显微镜等对在不同气氛中退火前后的Cd Si P_2晶体进行了组分元素、红外吸收系数以及红外透过均匀性测试,根据红外显微镜Mapping图像的标...采用改进的布里奇曼法生长出Cd Si P_2单晶体,运用X射线能谱仪、傅里叶变换红外分光光度计以及红外显微镜等对在不同气氛中退火前后的Cd Si P_2晶体进行了组分元素、红外吸收系数以及红外透过均匀性测试,根据红外显微镜Mapping图像的标准差值评判了晶体的红外透过均匀性。研究结果表明,经真空、Cd Si P_2粉末包裹、P/Cd（原子比为2：1）、Cd气氛等退火后,晶体组分元素的化学计量比、红外吸收系数和红外光学均匀性都得到了不同程度的改善,其中在1.29～2.00μm,经Cd Si P_2粉末包裹退火后的晶体吸收系数改善显著,在1.92～1.98μm波段的红外透过均匀性提高了14.06%;而在Cd气氛下退火后晶体的吸收系数在2.00～6.50μm波段降低最为明显,在2.70～2.78μm波段红外透过均匀性提高了17.43%。分析讨论了在上述波段中引起晶体红外吸收和红外透过不均匀性的主要因素,研究出较为有效的Cd Si P_2晶体退火工艺。展开更多
文摘[目的/意义]展现一个学科与其他学科之间知识吸收和知识扩散的完整过程,揭示学科知识发展的来龙去脉。[方法/过程]利用国际权威引文数据库Web of Science,依据Web of Science学科类别划分标准,以Information Science&Library Science(以下简称ISLS)为例,选择2006-2010年和2011-2015年两个时间段,建立三个数据集(施引文献集、原始文献集、被引文献集),利用双图叠加可视化的方式探析ISLS与其他学科在期刊层面的知识吸收过程;利用知识扩散广度、强度和速度三个指标揭示ISLS与其他学科之间知识扩散现状和发展趋势。[结果/结论]在两个阶段内,ISLS主要吸收期刊类群心理、教育、社会的知识,知识来源学科主要为计算机科学学和管理学,在相应时间段内,知识扩散广度与时间呈对数函数关系,知识扩散速度与时间呈指数函数关系,ISLS向其他学科的知识扩散强度呈幂率分布。
基金financially supported by National Natural Science Foundation of China(No.51803055)Natural Science Foundation of Hunan Province(No.2019JJ50372)Hunan Provincial Key Research and Development Program(No.2018GK2062)。
文摘MoS_(2)nanosheets(NSs)are novel 2D nanomaterials(NMs)with potential uses in many areas,and therefore oral exposure route to MoS_(2)NSs is plausible.Currently,MoS_(2)NSs are considered as biocompatible NMs,but there is lacking of systemic investigations to study the interactions of MoS_(2)NSs with intestinal cells.In this study,we exposed the 3D Caco-2 spheroids to MoS_(2)NSs or MoS_(2)powders(denoted as MoS_(2)-bulk),and investigated the potential adverse effects of MoS_(2)-materials based on transcriptomics and lipidomics analysis.As expected,both MoS_(2)NSs and MoS_(2)-bulk were dose-dependently internalized into 3D Caco-2 spheroids but did not induce cytotoxicity,membrane disruption or decrease of thiols.However,the Gene Ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analysis indicated that nutrient absorption and metabolism was decreased.One of the most significantly decreased KEGG pathways is fat digestion and absorption(map04975),and Western blotting analysis further showed that fatty acid binding protein 1 and apolipoprotein A1,key proteins involved in fat digestion and absorption,were down-regulated by MoS_(2)NSs or MoS_(2)-bulk.In addition,BODIPY 493/503 staining suggested that exposure to MoS_(2)NSs and MoS_(2)-bulk decreased lipid levels in the spheroids.However,lipidomics data indicated that MoS_(2)materials only decreased 8 lipid classes,including lysophosphatidylcholine,lysodimethylphosphatidylethanolamine,N-acylethanolamine,ceramide phosphoethanolamines,gangliosides,lysosphingomyelin and sulfatide,whereas most of the lipid classes were indeed increased.In addition,MoS_(2)NSs was more potent to decrease the lipid classes compared with MoS_(2)-bulk.Combined,the results from this study showed that MoS_(2)NSs and bulk materials were non-cytotoxic but altered lipid profiles in 3D Caco-2 spheroids.
文摘采用改进的布里奇曼法生长出Cd Si P_2单晶体,运用X射线能谱仪、傅里叶变换红外分光光度计以及红外显微镜等对在不同气氛中退火前后的Cd Si P_2晶体进行了组分元素、红外吸收系数以及红外透过均匀性测试,根据红外显微镜Mapping图像的标准差值评判了晶体的红外透过均匀性。研究结果表明,经真空、Cd Si P_2粉末包裹、P/Cd（原子比为2：1）、Cd气氛等退火后,晶体组分元素的化学计量比、红外吸收系数和红外光学均匀性都得到了不同程度的改善,其中在1.29～2.00μm,经Cd Si P_2粉末包裹退火后的晶体吸收系数改善显著,在1.92～1.98μm波段的红外透过均匀性提高了14.06%;而在Cd气氛下退火后晶体的吸收系数在2.00～6.50μm波段降低最为明显,在2.70～2.78μm波段红外透过均匀性提高了17.43%。分析讨论了在上述波段中引起晶体红外吸收和红外透过不均匀性的主要因素,研究出较为有效的Cd Si P_2晶体退火工艺。
