Abrin toxin(AT)and ricin toxin(RT)are highly toxic,easily accessible,and pose significant public health risks due to their potential for food and water contamination.In this study,we developed highly specific antibody...Abrin toxin(AT)and ricin toxin(RT)are highly toxic,easily accessible,and pose significant public health risks due to their potential for food and water contamination.In this study,we developed highly specific antibody pairs against AT(9D10-1B8)and RT(9G11-2B7),achieving impressive limits of detection(LODs)of 0.97 ng/mL and 0.45 ng/mL,respectively.Leveraging these antibodies,we constructed a dual-channel gold nanoparticle-based immunochromatographic assay(DC-AuNP-ICA)for the rapid,simultaneous detection of AT and RT in food and water samples.The assay demonstrated visual limits of detection(vLODs)of 5 ng/mL for AT and 2 ng/mL for RT,with calculated limits of detection(cLODs)of 3.66 ng/mL and 1.59 ng/mL,respectively.Recovery tests in spiked food and lake water samples yielded results of 93.78-105.70%,with coefficients of variation between 4.05%and 8.62%,confirming high accuracy and reproducibility.The DC-AuNP-ICA provides a reliable,on-site detection tool,offering a practical solution to mitigate the risks posed by AT and RT to public safety.展开更多
基金supported by the Natural Science Foundation of Jiangsu Province,China(BK20230043,BK2024304,BK20221088,BK20221058,CX(22)1013,KJ2025008,M20221006)the Major Science and Technology Projects,China(110202201020,LS-04)+1 种基金National Key R&D Program,China(2022YFF1101002)National Natural Science Foundation of China(22476067,22404066,22361132536,22306074,22236002,22207045).
文摘Abrin toxin(AT)and ricin toxin(RT)are highly toxic,easily accessible,and pose significant public health risks due to their potential for food and water contamination.In this study,we developed highly specific antibody pairs against AT(9D10-1B8)and RT(9G11-2B7),achieving impressive limits of detection(LODs)of 0.97 ng/mL and 0.45 ng/mL,respectively.Leveraging these antibodies,we constructed a dual-channel gold nanoparticle-based immunochromatographic assay(DC-AuNP-ICA)for the rapid,simultaneous detection of AT and RT in food and water samples.The assay demonstrated visual limits of detection(vLODs)of 5 ng/mL for AT and 2 ng/mL for RT,with calculated limits of detection(cLODs)of 3.66 ng/mL and 1.59 ng/mL,respectively.Recovery tests in spiked food and lake water samples yielded results of 93.78-105.70%,with coefficients of variation between 4.05%and 8.62%,confirming high accuracy and reproducibility.The DC-AuNP-ICA provides a reliable,on-site detection tool,offering a practical solution to mitigate the risks posed by AT and RT to public safety.
