Abortive transcript(AT)is a 2-19 nt long non-coding RNA that is produced in the abortive initiation stage.Abortive initiation was found to be closely related to RNA polymerase through in vitro experiments.Therefore,th...Abortive transcript(AT)is a 2-19 nt long non-coding RNA that is produced in the abortive initiation stage.Abortive initiation was found to be closely related to RNA polymerase through in vitro experiments.Therefore,the distribution of AT length and the scale of abortive initiation are correlated to the promoter,discriminator,and transcription initiation sequence,and can be affected by transcription elongation factors.AT plays an important role in the occurrence and development of various diseases.Here we summarize the discovery of AT,the factors responsible for AT formation,the detection methods and biological functions of AT,to provide new clues for finding potential targets in the early diagnosis and treatment of cancers.展开更多
Somatic hybridization is performed to obtain significant cytoplasmic male sterility （CMS） lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to...Somatic hybridization is performed to obtain significant cytoplasmic male sterility （CMS） lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-IA, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-IB, and the abortive process of SaNa-IA were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-IA was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-IA aborted in the pollen mother cell （PMC） stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-IA is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.展开更多
The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food security.H...The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food security.However,the molecular mechanisms underlyingfertility abortion and the restoration of CMs-WA lines remain largely elusive.In this study,we cloned a restorer gene,Rf2o,following a genome-wide association study analysis of the core parent lines of three-line hybrid rice.We found that Rf20 was present in all core parental lines,but different haplotypes and structural variants of its gene resulted in differences in Rf2o expression levels between sterileand restored lines.Rf20 could restorepollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMs lines under high temperatures.In addition,we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11.This interaction enhances Cox11's function as a scavenger of reactive oxygen species,which in turn restores pollenfertility.Collectively,ourstudysuggestsanewactionmodeforpentatricopeptide repeatproteins inthe fertilityrestorationofCMs lines,providing an essential theoretical basisforbreedingrobustrestorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.展开更多
Objective:To compare the clinical efficacy of mifepristone-misoprostol medical management versus surgical curettage for first-trimester missed miscarriage,and to establish evidence-based sonographic cutoff values pred...Objective:To compare the clinical efficacy of mifepristone-misoprostol medical management versus surgical curettage for first-trimester missed miscarriage,and to establish evidence-based sonographic cutoff values predictive of incomplete abortion requiring surgical intervention.Methods:We retrospectively analyzed a cohort of 702 women diagnosed with first-trimester missed miscarriage between January 2020 and May 2023.Demographic characteristics and ultrasound parameters were systematically recorded.Receiver operating characteristic(ROC)curve analysis was performed to establish optimal sonographic cutoff values for predicting incomplete abortion requiring surgical intervention.Results:146 patients received medical treatment(mifepristone and misoprostol)and 556 underwent surgical curettage.At the 1-month follow-up,the medical group showed significantly greater endometrial thickness and longer postoperative bleeding duration than the surgical group(P<0.05).The menstrual volume reduction rate(23.56%)was significantly lower in the medical group than in the surgical group.The incomplete abortion rate was higher in the medical group(17.12%,25/146)than in the surgical group(2.88%,16/556).Among the medical group,14 patients(9.59%)required curettage due to incomplete abortion,while 11 cases resolved spontaneously after prolonged medication.ROC curve analysis identified two cut-off values indicating the need for surgical intervention:endometrial thickness>1.21 cm at 24 h post-medical abortion,and residual mass diameter>0.95 cm at 7 days post-medical abortion.Conclusions:Medical management of first-trimester missed miscarriage using mifepristone-misoprostol demonstrates comparable efficacy to surgical curettage.An endometrial thickness>1.21 cm at 24 h or residual tissue diameter>0.95 cm at 7 days post-medical abortion should prompt consideration of incomplete abortion.展开更多
Background: Some studies have indicated a potential link between a history of induced abortion (IA) and the subsequent risk of gestational diabetes mellitus (GDM), but the relationship is not fully understood, and the...Background: Some studies have indicated a potential link between a history of induced abortion (IA) and the subsequent risk of gestational diabetes mellitus (GDM), but the relationship is not fully understood, and the aim of this study was to further elucidate the association. Methods: The case-control study was conducted at 2 hospitals in central China from April 2018 to October 2020. GDM was diagnosed by an oral glucose tolerance test (OGTT). Information on history of IA was obtained through a face-to-face interview. Results: Among 396 GDM cases and 904 controls, the proportion of participants with history of IA in the case group was 30.6%, which was higher than that in the control group (23.1%), and the difference was statistically significant (p = 0.005). After adjusting for potential confounders, women with a history of IA had an increased subsequent risk of GDM compared with women without (OR, 1.24, 95% CI, 1.10 - 1.40, p = 0.002). The subsequent risk of GDM in pregnant women increased as the number of previous IAs increased (p for trend was equal to 0.004). Stratified analysis showed that women with a history of medical abortion (OR, 1.28, 95% CI, 1.01 - 1.62, p = 0.048) or surgical abortion (OR, 1.20, 95% CI, 1.04 - 1.38, p = 0.024) both had an increased subsequent risk of GDM compared with women without. Conclusion: History of IA, either medical or surgical, was related to an increased risk of GDM in subsequent pregnancy. The greater the number of previous IAs, the greater the subsequent risk of GDM.展开更多
[Objectives]To explore the effect of selenium(Se)on inhibiting embryo abortion and enhancing seedling cultivation quality of Red sandalwood(Pterocarpus santalinus).[Methods]Based on prior cultivation practices and exp...[Objectives]To explore the effect of selenium(Se)on inhibiting embryo abortion and enhancing seedling cultivation quality of Red sandalwood(Pterocarpus santalinus).[Methods]Based on prior cultivation practices and experimental research,three categories comprising 13 forest soil nutrient management schemes were designed to investigate the synergistic effects of Se,NPK compound fertilizers,and enzyme-microbe fermented organic fertilizers(EFOF)on embryo abortion,winged pod development,and seedling quality of Red sandalwood.[Results]Increasing the Se content in the soil,particularly in the form of selenite/Se(IV),within one month following the harvest of Red sandalwood pods and within two months prior to flower withering,significantly reduced embryo abortion percentage(EAP),and consequently improved seed quality and yield per plant.The effect of Se application was markedly greater than that of the single application of nitrogen(N),phosphorus(P),potassium(K),boron(B)fertilizers,or organic fertilizers.Furthermore,when Se was applied in combination with NPK compound fertilizers and EFOF,these beneficial effects were significantly enhanced.When Se(IV)was applied individually,the EAP decreased by 62.4%,reaching 24.8% at 8 weeks after flower withering(compared to 65.9%in the unmanaged control,UMC).Following winged pod maturation,the percentage of empty winged pods(PEWP)declined by 65.2% to 16.8%(UMC:48.2%),the average individual winged pod weight(IWPW)increased by 69.1%to 0.690 g per fruit(UMC:0.408 g),and the winged pod yield(WPY)rose by 214.8% to 4.03 kg(UMC:1.28 kg).Additionally,the blasted seed percentage(BSP)was reduced by 51.2% to 29.9%(UMC:61.3%),and the 100-seed weight(HSW)increased by 96.0%to 8.37 g(UMC:4.27 g).Following sowing in the nursery,the seedling emergence rate(SER)increased by 6.57-fold,reaching 59.8%(UMC:7.9%).Additionally,the whole plant biomass of 6-month-old seedlings increased by 52.9%,attaining 1.56 g(UMC:1.02 g).The combined application of EFOF+NPK+Se(IV)significantly reduced the EAP,PEWP,and BSP by 56.5%,46.0%,and 56.3%,respectively,compared to the single application of Se(IV).Furthermore,these percentages decreased by 79.7%,78.9%,and 71.8%,respectively,relative to the single application of NPK compound fertilizers,and by 79.0%,74.5%,and 72.1%,respectively,compared to the single application of EFOF.Additionally,the SER increased by 34.6%,141.0%,and 287.0%,respectively,when compared to the single application of Se(IV),NPK compound fertilizers,and EFOF.[Conclusions]Enhancing the nutrient status of forest soils,particularly the concentration of Se(IV),constitutes a critical technical approach to improving the resistance of Red sandalwood to low-temperature stress during its flowering and fruiting stages,thereby preventing embryo abortion.展开更多
Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear....Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.展开更多
Young women’s physical and mental health is seriously impacted by recurrent spontaneous abortion(RSA),a prevalent obstetric complication that is becoming more commonplace worldwide.Therefore,a thorough investigation ...Young women’s physical and mental health is seriously impacted by recurrent spontaneous abortion(RSA),a prevalent obstetric complication that is becoming more commonplace worldwide.Therefore,a thorough investigation into the pathophysiology of RSA and the development of novel therapeutic strategies are imperative.Recent developments suggest that mesenchymal stem cell(MSC)-based therapies may be viable for addressing RSA.Through a variety of mechanisms,the immunological circumstances at the maternal-fetal contact can be altered,including regulating immune cell homeostasis,enhancing immune tolerance,alleviating inflammatory responses,promoting angiogenic processes,and promoting tissue regeneration.MSCs exhibit a remarkable capacity for multidifferentiation that could enhance pregnancy outcomes.This article provides compelling studies supporting the efficacy of MSC-based therapies in improving pregnancy outcomes in women with RSA.展开更多
Abortion is one of the leading causes of maternal death in Cameroon, despite numerous policies and strategies put in place to reduce it by 2030.This study aims to describe the epidemiologic aspects of abortions within...Abortion is one of the leading causes of maternal death in Cameroon, despite numerous policies and strategies put in place to reduce it by 2030.This study aims to describe the epidemiologic aspects of abortions within hospitals in the city of Yaoundé in 2023. Methods: This was a descriptive cross-sectional study with prospective data collection in the maternity wards of the Yaoundé Gynaeco-Obstetric and Paediatric Hospital, and the Yaoundé Central Hospital for 7 months, from the 1st of November 2022 to the 31st of May 2023. The variables studied were demographic and clinical characteristics, means of recourse and post-abortion contraception. The frequency of abortions was calculated as the ratio of the number of abortions to the number of deliveries. The calculation of numbers and frequencies was used to describe the study population, and to highlight the sociodemographic and clinical characteristics of the patients recruited. Quantitative variables were expressed as mean ± standard deviation. Data were collected numerically and classified according to type, then analyzed using Epi-info version 7.2 software. Microsoft Office Excel 2013 was used to produce figures and tables. Results: During the study period, 169 patients were selected out of 1882 patients who had consulted for gynaecological emergencies;but only 164 of them accepted to participate. The frequency of abortions was 9.6% (169/1882). The age group of 25 to 29 years was the most represented, with 29.3% (48/164). They were mainly single 76.2% (125/164), and housewives 33.5% (55/164). The notion of previous abortion was found in 30.5% (50/164) of them. Approximately 19.5% of patients (32/164) reported to have conceived while using contraception, 56.2% of whom (18/32) used male condoms. At the time of admission to the emergency unit, the predominant symptom was bleeding in 70.7% of cases. The abortions were mainly spontaneous, in 75% of cases (123/164). In cases of induced abortions, 24.4% (10/41) of them took place after 14 weeks. Unexpected pregnancy was the main reason for termination in 46.3% of cases (19/41). Regarding induced abortion, 58.54% (24/41) of them resorted to voluntary medical termination. The mortality rate was 1.2%. The acceptability rate of a modern contraceptive method before discharge was 31.7%. Conclusion: Although frequent in our environment, data relating to abortion remain under-evaluated. Abortions occur mainly among young, single women, with a still very high proportion of induced abortions. The acceptability of post-abortion contraception remains poor. Prevention of unwanted pregnancies and risk factors is necessary to reduce the burden of abortions in low-income countries.展开更多
Background:Achromobacter xylosoxidans(A.xylosoxidans)subspecies strains are known to be opportunistic environmental inhabitants.Among the two species,A.xylosoxidans ssp.xylosoxidans is more commonly reported cause of ...Background:Achromobacter xylosoxidans(A.xylosoxidans)subspecies strains are known to be opportunistic environmental inhabitants.Among the two species,A.xylosoxidans ssp.xylosoxidans is more commonly reported cause of nosocomial infections colonizing the hospital environment and medical devices,while A.xylosoxidans ssp.denitrificans(AD)strains are widely distributed in the abiotic environment.The present retrospective observational study was aimed at understanding the occurrence of AD infections in the Bareilly region,and to look into the effective herbal and conventional antimicrobial resistance profile of the strains identified at the laboratory.Methods:The present retrospective study analysed Clinical Microbiology laboratory data of Indian veterinary research Institute.The data for the last 14 years(2011-2024)was retrieved,tabulated and analysed using MS Excel program to determine significance of occurrence,and variation in antimicrobial resistance of the strains isolated from different sources usingχ2 and odds ratio analysis.Results:The study revealed that AD was detectable as a potential pathogen not only from environmental samples but also from 51 clinical cases(either as pure culture or mixed infection),and also from healthy humans and animals.The pathogen was most commonly associated with deaths in animals and birds due to septicaemia and was isolated is single pathogen from blood samples.It was also detected as single pathogen from cases of abortions,metritis,and urinary tract infections.However,from cases of haemorrhagic enteritis,diarrhoea,mastitis,wound infections,pyoderma and abscesses,and middle ear infections AD was isolated in association of one or more potentially pathogenic bacteria.Of the 80 isolates in the study,68 had multiple drug resistance,and 21 produced metallo-β-lactamases responsible for resistance against most of theβ-lactam antibiotics,including cephalosporins and carbapenems.The most effective antibiotic was gentamicin,inhibiting 90.67%of the isolates,followed by tigecycline(85.00%),ciprofloxacin(80.77%),piperacillin tazobactam(80.65%);other antibiotics were effective against less than 80%of the isolates.Among the herbal antimicrobials,cinnamaldehyde,cinnamon oil,carvacrol,and ajowan oil inhibited 98.41%,85.07%,85.00%,and 83.75%of the isolates,respectively.Conclusion:The study concluded that in the Bareilly region in India,multiple-drug-resistance AD may be an emerging pathogen prevalent in environment and apparently healthy animals.More studies are warranted to understand the AD strains at molecular level to understand their zoonotic potential and circulation in the environment.展开更多
Francophone countries in sub-Saharan Africa had been more resistant to induced abortion.Their cultural heritage from France had been influential in strengthening their pronatalist position regarding contraception and ...Francophone countries in sub-Saharan Africa had been more resistant to induced abortion.Their cultural heritage from France had been influential in strengthening their pronatalist position regarding contraception and abortion.Most of them inherited the judicial system and cultural value from France,which have been transmitted to Francophone countries.However,cultural and behavioral changes in reproductive health have begun in Francophone sub-Saharan Africa since the mid-1990s after the paradigm shift on population policy with the International Conference on Population and Development(ICPD)in 1994.Some women have started to use contraception for birth limitation.Induced abortion to prevent unintended pregnancy has been increasingly liberalized in some settings.In this study,the changing contexts for induced abortion in Francophone countries will be reviewed.Recent step undertaken by Benin to legalize abortion in most circumstances shows that Francophone countries may be overcoming their cultural barriers and adapting universal human rights based reproductive behaviors.展开更多
Objective Heat wave exposure significantly impacts human health.Nevertheless,studies on the long-term effects of heat wave exposure during adolescence on adverse pregnancy outcomes(APOs)are rare.This study aimed to in...Objective Heat wave exposure significantly impacts human health.Nevertheless,studies on the long-term effects of heat wave exposure during adolescence on adverse pregnancy outcomes(APOs)are rare.This study aimed to investigate the relationship between the long-term effects of heat wave exposure during adolescence and APOs.Methods We analyzed data from 3,376 female and 3,013 male participants across 31 provinces in China.All adolescents(10–19 years old),early adolescents(10–14),and late adolescents(15–19)were chosen as exposure windows.Heat waves were defined as periods lasting 2‒4 consecutive days with the daily temperature exceeding the 75th,90th,and 92.5th percentiles.We employed multivariate logistic regression models to assess the associations between exposure to heat waves during adolescence and APOs.Results The results revealed significant associations between male exposure to heat wave events during late adolescence and spontaneous abortion(P<0.05),which was more pronounced in South China.In contrast,no statistically significant associations were detected between males’exposure to heat wave events during adolescence and their partners’preterm birth(P>0.05 for all comparisons).The exposure of females to heat waves during adolescence was not significantly associated with subsequent spontaneous abortion or preterm birth(P>0.05 for all comparisons).Conclusions This study demonstrates that spontaneous abortion in females is associated with heat wave exposure in their male partner during adolescence.展开更多
Background: Unsafe abortion (USA) is defined as the termination of a non-desired pregnancy, performed by an unqualified person or in an environment without minimum medical standards, or both. It can impact negatively ...Background: Unsafe abortion (USA) is defined as the termination of a non-desired pregnancy, performed by an unqualified person or in an environment without minimum medical standards, or both. It can impact negatively the fertility in the future by tubo-peritoneal lesions. The current study aimed to highlight the infertility factors associated with USA. Material and Methods: From January 1st 2000 to December 31st 2021, a total of 3876 patients that sought care for infertility in the University Clinic of Kinshasa and the Edith Medical Center were enrolled. The socio-demographic, clinical and paraclinical variables were recorded and analyzed in relation to USA history. Results: The patients’ average age was 33.4 ± 5.7 years. Half of them were housewives;about sixty percent of the patients were nulliparous (59.31%). Seventy percent had a secondary infertility with 43.33% having a history of unsafe abortion. The main diagnosis was genital infections and uterus myoma. The chronic endometritis, bilateral tubal occlusion and Uterine synechiae were more associated with USA group. The spermogram anomalies and other partners’ lesions seemed of infectious origin. Conclusion: The USA remains a more frequent phenomenon in our setting. The chronic endometritis, bilateral tubal occlusion, uterine synechiae and indirectly uterus myoma were the infertility factors related to USA. The genital infection of any origin seems to be the main denominator underlying the female and the male infertility in our setting.展开更多
[Objectives]This study was conducted to investigate the regulatory effects of selenium(Se)on the content and balance of endogenous hormones and the function of antioxidant system during seed development in Red sandalw...[Objectives]This study was conducted to investigate the regulatory effects of selenium(Se)on the content and balance of endogenous hormones and the function of antioxidant system during seed development in Red sandalwood(Pterocarpus santalinus).[Methods]Two basic treatments,seven single-fertilization treatments,and four combined fertilization treatments were designed.Sampling was conducted at 2,5,8,and 18 weeks after flower withering to measure the embryo abortion percentage(EAP),the contents of three endogenous hormones(IAA,GA_(3),ABA),and the activities of four antioxidant enzymes(CAT,APX,SOD,GR).[Results]Se application significantly inhibited embryo abortion in Red sandalwood,with Na_(2)SeO_(3)[Se(IV)]showing superior effects to Na_(2)SeO_(3)(Se(VI))and far exceeding the efficacy of individual applications of KCl,H_(3)BO_(3),CO(NH_(2))_(2),Ca(H_(2)PO_(4))_(2),NPK compound fertilizer,or EFOF.The combined treatment of Se with NPK compound fertilizer and EFOF[EFOF+NPK compound fertilizer+Se(IV)]was the most effective,reducing the abortion percentage by 77.8%compared with UMC at 18 weeks after flower withering.Se application significantly increased the levels of three endogenous hormones and the(IAA+GA_(3))/ABA ratio in Red sandalwood seeds(including the embryonic stage).In the optimal treatment,the(IAA+GA_(3))content was 240.7%,256.4%,353.7%,and 502.9%higher than that of UMC at 2,5,8,and 18 weeks after flower withering,respectively.Se application also concurrently enhanced antioxidant enzyme activities,with all four antioxidant enzymes in seeds of Se-treated plants showing significant increases.Notably,the selenoenzyme GR maintained considerably high activity even at 18 weeks after flower withering.The EAP was highly significantly negatively correlated with IAA content and GR activity,identifying IAA and(IAA+GA_(3))content as key hormonal indicators and GR as the core antioxidant enzyme,together constituting the central regulatory factors.The results indicate that Se suppresses embryo abortion in Red sandalwood through a dual regulatory pathway:by elevating IAA and GA_(3)levels along with the(IAA+GA_(3))/ABA ratio to optimize hormonal signaling networks,and by enhancing the activities of antioxidant enzymes such as GR to alleviate oxidative stress induced by cool-season low temperatures.[Conclusions]This study provides a theoretical basis and technical strategy for precision fertilization and stress resistance management in the cultivation of Red sandalwood.展开更多
Objective:To assess comprehensive abortion care(CAC)services uptake and barriers to it in selected representative units of the three tier health care in the district of Malda in West Bengal,India.Methods:We conducted ...Objective:To assess comprehensive abortion care(CAC)services uptake and barriers to it in selected representative units of the three tier health care in the district of Malda in West Bengal,India.Methods:We conducted community-based,concurrent,parallel mixed methods study.Quantitative assessment of knowledge of primary health care workers on CAC and appropriate infrastructure assessment was done.Qualitative methods were used to identify themes affecting uptake of quality care among stakeholders.Results:A total of 48 staff nurses and 133 auxiliary nurses and midwives were included in the study.About half of nurses(of total 48)who were second tier health care provider and two thirds(of total 133)of first tier health care provider had incorrect knowledge about legality of abortion.Only 40%mothers seeking abortion were accompanied to subcentre by primary health care worker.At the third tier health care site,physicians insisted on necessity of refresher training.Suboptimal uptake of available CAC services was due to lack of enabling environment.Lack of updated training across health care workers was observed.Conclusions:Health care providers at the community level were unaware of facilities available for mothers seeking abortion.Higher level health care providers were unsure of specific knowledge about legal permissibility and sterilization of apparatus for abortion.At the highest level,more training on medical methods of abortion was required.The problems identified are amenable to correction,and some administrative and service-related provision on appropriate measures may lead to immediate success.展开更多
The Chaling wild abortive type （CL-type） cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical ...The Chaling wild abortive type （CL-type） cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical abortive pollens, and showed the characteristics of good outcrossing with a stigma exsertion rate of 48.2% and an outcrossing rate varied from 30% to 50%, good combining ability, and fine grain quality. E-Jing A was sensitive to temperature. Its duration from sowing to heading was 78-90 d when it was sown from mid May to early June at Wuhan. The breeding procedure of E-Jing A and its characteristics, relationship of restoring and maintaining were introduced.展开更多
基金Supported by Key Science and Technology Research and Development Program Project of Guangxi,No.AB22035017.
文摘Abortive transcript(AT)is a 2-19 nt long non-coding RNA that is produced in the abortive initiation stage.Abortive initiation was found to be closely related to RNA polymerase through in vitro experiments.Therefore,the distribution of AT length and the scale of abortive initiation are correlated to the promoter,discriminator,and transcription initiation sequence,and can be affected by transcription elongation factors.AT plays an important role in the occurrence and development of various diseases.Here we summarize the discovery of AT,the factors responsible for AT formation,the detection methods and biological functions of AT,to provide new clues for finding potential targets in the early diagnosis and treatment of cancers.
基金the National Natural Science Foundation of China(31330057)the Priority Academic Program Development of Jiangsu Higher Education Institutions+1 种基金the Program of International S&T Cooperation of China(1021)the Jiangsu Province Graduate Innovation Fund(XCLX13_899),China
文摘Somatic hybridization is performed to obtain significant cytoplasmic male sterility （CMS） lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-IA, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-IB, and the abortive process of SaNa-IA were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-IA was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-IA aborted in the pollen mother cell （PMC） stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-IA is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.
基金Biological Breeding-Major Projects(2023ZD04076)National Key Research and Development Project(2022YFD1200800)+1 种基金Hunan Natural Science Foundation(2023JJ30442)Special Funds for the Construction of Innovative Provinces in Hunan Province(2021NK1002).
文摘The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food security.However,the molecular mechanisms underlyingfertility abortion and the restoration of CMs-WA lines remain largely elusive.In this study,we cloned a restorer gene,Rf2o,following a genome-wide association study analysis of the core parent lines of three-line hybrid rice.We found that Rf20 was present in all core parental lines,but different haplotypes and structural variants of its gene resulted in differences in Rf2o expression levels between sterileand restored lines.Rf20 could restorepollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMs lines under high temperatures.In addition,we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11.This interaction enhances Cox11's function as a scavenger of reactive oxygen species,which in turn restores pollenfertility.Collectively,ourstudysuggestsanewactionmodeforpentatricopeptide repeatproteins inthe fertilityrestorationofCMs lines,providing an essential theoretical basisforbreedingrobustrestorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.
基金supported by National Natural Science Foundation of China(Project approval number 82201825).
文摘Objective:To compare the clinical efficacy of mifepristone-misoprostol medical management versus surgical curettage for first-trimester missed miscarriage,and to establish evidence-based sonographic cutoff values predictive of incomplete abortion requiring surgical intervention.Methods:We retrospectively analyzed a cohort of 702 women diagnosed with first-trimester missed miscarriage between January 2020 and May 2023.Demographic characteristics and ultrasound parameters were systematically recorded.Receiver operating characteristic(ROC)curve analysis was performed to establish optimal sonographic cutoff values for predicting incomplete abortion requiring surgical intervention.Results:146 patients received medical treatment(mifepristone and misoprostol)and 556 underwent surgical curettage.At the 1-month follow-up,the medical group showed significantly greater endometrial thickness and longer postoperative bleeding duration than the surgical group(P<0.05).The menstrual volume reduction rate(23.56%)was significantly lower in the medical group than in the surgical group.The incomplete abortion rate was higher in the medical group(17.12%,25/146)than in the surgical group(2.88%,16/556).Among the medical group,14 patients(9.59%)required curettage due to incomplete abortion,while 11 cases resolved spontaneously after prolonged medication.ROC curve analysis identified two cut-off values indicating the need for surgical intervention:endometrial thickness>1.21 cm at 24 h post-medical abortion,and residual mass diameter>0.95 cm at 7 days post-medical abortion.Conclusions:Medical management of first-trimester missed miscarriage using mifepristone-misoprostol demonstrates comparable efficacy to surgical curettage.An endometrial thickness>1.21 cm at 24 h or residual tissue diameter>0.95 cm at 7 days post-medical abortion should prompt consideration of incomplete abortion.
文摘Background: Some studies have indicated a potential link between a history of induced abortion (IA) and the subsequent risk of gestational diabetes mellitus (GDM), but the relationship is not fully understood, and the aim of this study was to further elucidate the association. Methods: The case-control study was conducted at 2 hospitals in central China from April 2018 to October 2020. GDM was diagnosed by an oral glucose tolerance test (OGTT). Information on history of IA was obtained through a face-to-face interview. Results: Among 396 GDM cases and 904 controls, the proportion of participants with history of IA in the case group was 30.6%, which was higher than that in the control group (23.1%), and the difference was statistically significant (p = 0.005). After adjusting for potential confounders, women with a history of IA had an increased subsequent risk of GDM compared with women without (OR, 1.24, 95% CI, 1.10 - 1.40, p = 0.002). The subsequent risk of GDM in pregnant women increased as the number of previous IAs increased (p for trend was equal to 0.004). Stratified analysis showed that women with a history of medical abortion (OR, 1.28, 95% CI, 1.01 - 1.62, p = 0.048) or surgical abortion (OR, 1.20, 95% CI, 1.04 - 1.38, p = 0.024) both had an increased subsequent risk of GDM compared with women without. Conclusion: History of IA, either medical or surgical, was related to an increased risk of GDM in subsequent pregnancy. The greater the number of previous IAs, the greater the subsequent risk of GDM.
基金Supported by National Natural Science Foundation of China(31270674)Science and Technology Plan Project of Zhaoqing City(2019N012)National Undergraduate Science and Technology Innovation Training Program of China(202210580007).
文摘[Objectives]To explore the effect of selenium(Se)on inhibiting embryo abortion and enhancing seedling cultivation quality of Red sandalwood(Pterocarpus santalinus).[Methods]Based on prior cultivation practices and experimental research,three categories comprising 13 forest soil nutrient management schemes were designed to investigate the synergistic effects of Se,NPK compound fertilizers,and enzyme-microbe fermented organic fertilizers(EFOF)on embryo abortion,winged pod development,and seedling quality of Red sandalwood.[Results]Increasing the Se content in the soil,particularly in the form of selenite/Se(IV),within one month following the harvest of Red sandalwood pods and within two months prior to flower withering,significantly reduced embryo abortion percentage(EAP),and consequently improved seed quality and yield per plant.The effect of Se application was markedly greater than that of the single application of nitrogen(N),phosphorus(P),potassium(K),boron(B)fertilizers,or organic fertilizers.Furthermore,when Se was applied in combination with NPK compound fertilizers and EFOF,these beneficial effects were significantly enhanced.When Se(IV)was applied individually,the EAP decreased by 62.4%,reaching 24.8% at 8 weeks after flower withering(compared to 65.9%in the unmanaged control,UMC).Following winged pod maturation,the percentage of empty winged pods(PEWP)declined by 65.2% to 16.8%(UMC:48.2%),the average individual winged pod weight(IWPW)increased by 69.1%to 0.690 g per fruit(UMC:0.408 g),and the winged pod yield(WPY)rose by 214.8% to 4.03 kg(UMC:1.28 kg).Additionally,the blasted seed percentage(BSP)was reduced by 51.2% to 29.9%(UMC:61.3%),and the 100-seed weight(HSW)increased by 96.0%to 8.37 g(UMC:4.27 g).Following sowing in the nursery,the seedling emergence rate(SER)increased by 6.57-fold,reaching 59.8%(UMC:7.9%).Additionally,the whole plant biomass of 6-month-old seedlings increased by 52.9%,attaining 1.56 g(UMC:1.02 g).The combined application of EFOF+NPK+Se(IV)significantly reduced the EAP,PEWP,and BSP by 56.5%,46.0%,and 56.3%,respectively,compared to the single application of Se(IV).Furthermore,these percentages decreased by 79.7%,78.9%,and 71.8%,respectively,relative to the single application of NPK compound fertilizers,and by 79.0%,74.5%,and 72.1%,respectively,compared to the single application of EFOF.Additionally,the SER increased by 34.6%,141.0%,and 287.0%,respectively,when compared to the single application of Se(IV),NPK compound fertilizers,and EFOF.[Conclusions]Enhancing the nutrient status of forest soils,particularly the concentration of Se(IV),constitutes a critical technical approach to improving the resistance of Red sandalwood to low-temperature stress during its flowering and fruiting stages,thereby preventing embryo abortion.
基金supported by the China Agricultural Research System(CARS-23-G15)the Innovation Team of Henan Academy of Agricultural Sciences,China(2024TD06)+2 种基金the Autonomous Innovation Project of Henan Academy of Agricultural Sciences,China(2024ZC034)the Joint Research on Agricultural Variety Improvement of Henan Province,China(2022010504)the Key Research&Development Project of Henan Province,China(221111110100)。
文摘Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.
基金Supported by the Natural Science Foundation of Liaoning Province,No.2024-MSLH-525.
文摘Young women’s physical and mental health is seriously impacted by recurrent spontaneous abortion(RSA),a prevalent obstetric complication that is becoming more commonplace worldwide.Therefore,a thorough investigation into the pathophysiology of RSA and the development of novel therapeutic strategies are imperative.Recent developments suggest that mesenchymal stem cell(MSC)-based therapies may be viable for addressing RSA.Through a variety of mechanisms,the immunological circumstances at the maternal-fetal contact can be altered,including regulating immune cell homeostasis,enhancing immune tolerance,alleviating inflammatory responses,promoting angiogenic processes,and promoting tissue regeneration.MSCs exhibit a remarkable capacity for multidifferentiation that could enhance pregnancy outcomes.This article provides compelling studies supporting the efficacy of MSC-based therapies in improving pregnancy outcomes in women with RSA.
文摘Abortion is one of the leading causes of maternal death in Cameroon, despite numerous policies and strategies put in place to reduce it by 2030.This study aims to describe the epidemiologic aspects of abortions within hospitals in the city of Yaoundé in 2023. Methods: This was a descriptive cross-sectional study with prospective data collection in the maternity wards of the Yaoundé Gynaeco-Obstetric and Paediatric Hospital, and the Yaoundé Central Hospital for 7 months, from the 1st of November 2022 to the 31st of May 2023. The variables studied were demographic and clinical characteristics, means of recourse and post-abortion contraception. The frequency of abortions was calculated as the ratio of the number of abortions to the number of deliveries. The calculation of numbers and frequencies was used to describe the study population, and to highlight the sociodemographic and clinical characteristics of the patients recruited. Quantitative variables were expressed as mean ± standard deviation. Data were collected numerically and classified according to type, then analyzed using Epi-info version 7.2 software. Microsoft Office Excel 2013 was used to produce figures and tables. Results: During the study period, 169 patients were selected out of 1882 patients who had consulted for gynaecological emergencies;but only 164 of them accepted to participate. The frequency of abortions was 9.6% (169/1882). The age group of 25 to 29 years was the most represented, with 29.3% (48/164). They were mainly single 76.2% (125/164), and housewives 33.5% (55/164). The notion of previous abortion was found in 30.5% (50/164) of them. Approximately 19.5% of patients (32/164) reported to have conceived while using contraception, 56.2% of whom (18/32) used male condoms. At the time of admission to the emergency unit, the predominant symptom was bleeding in 70.7% of cases. The abortions were mainly spontaneous, in 75% of cases (123/164). In cases of induced abortions, 24.4% (10/41) of them took place after 14 weeks. Unexpected pregnancy was the main reason for termination in 46.3% of cases (19/41). Regarding induced abortion, 58.54% (24/41) of them resorted to voluntary medical termination. The mortality rate was 1.2%. The acceptability rate of a modern contraceptive method before discharge was 31.7%. Conclusion: Although frequent in our environment, data relating to abortion remain under-evaluated. Abortions occur mainly among young, single women, with a still very high proportion of induced abortions. The acceptability of post-abortion contraception remains poor. Prevention of unwanted pregnancies and risk factors is necessary to reduce the burden of abortions in low-income countries.
基金supported by grants from CAAST-ACLH(NAHEP/CAAST/2018-19)of the ICAR-World Bank-funded National Agricultural Higher Education Project(NAHEP).
文摘Background:Achromobacter xylosoxidans(A.xylosoxidans)subspecies strains are known to be opportunistic environmental inhabitants.Among the two species,A.xylosoxidans ssp.xylosoxidans is more commonly reported cause of nosocomial infections colonizing the hospital environment and medical devices,while A.xylosoxidans ssp.denitrificans(AD)strains are widely distributed in the abiotic environment.The present retrospective observational study was aimed at understanding the occurrence of AD infections in the Bareilly region,and to look into the effective herbal and conventional antimicrobial resistance profile of the strains identified at the laboratory.Methods:The present retrospective study analysed Clinical Microbiology laboratory data of Indian veterinary research Institute.The data for the last 14 years(2011-2024)was retrieved,tabulated and analysed using MS Excel program to determine significance of occurrence,and variation in antimicrobial resistance of the strains isolated from different sources usingχ2 and odds ratio analysis.Results:The study revealed that AD was detectable as a potential pathogen not only from environmental samples but also from 51 clinical cases(either as pure culture or mixed infection),and also from healthy humans and animals.The pathogen was most commonly associated with deaths in animals and birds due to septicaemia and was isolated is single pathogen from blood samples.It was also detected as single pathogen from cases of abortions,metritis,and urinary tract infections.However,from cases of haemorrhagic enteritis,diarrhoea,mastitis,wound infections,pyoderma and abscesses,and middle ear infections AD was isolated in association of one or more potentially pathogenic bacteria.Of the 80 isolates in the study,68 had multiple drug resistance,and 21 produced metallo-β-lactamases responsible for resistance against most of theβ-lactam antibiotics,including cephalosporins and carbapenems.The most effective antibiotic was gentamicin,inhibiting 90.67%of the isolates,followed by tigecycline(85.00%),ciprofloxacin(80.77%),piperacillin tazobactam(80.65%);other antibiotics were effective against less than 80%of the isolates.Among the herbal antimicrobials,cinnamaldehyde,cinnamon oil,carvacrol,and ajowan oil inhibited 98.41%,85.07%,85.00%,and 83.75%of the isolates,respectively.Conclusion:The study concluded that in the Bareilly region in India,multiple-drug-resistance AD may be an emerging pathogen prevalent in environment and apparently healthy animals.More studies are warranted to understand the AD strains at molecular level to understand their zoonotic potential and circulation in the environment.
文摘Francophone countries in sub-Saharan Africa had been more resistant to induced abortion.Their cultural heritage from France had been influential in strengthening their pronatalist position regarding contraception and abortion.Most of them inherited the judicial system and cultural value from France,which have been transmitted to Francophone countries.However,cultural and behavioral changes in reproductive health have begun in Francophone sub-Saharan Africa since the mid-1990s after the paradigm shift on population policy with the International Conference on Population and Development(ICPD)in 1994.Some women have started to use contraception for birth limitation.Induced abortion to prevent unintended pregnancy has been increasingly liberalized in some settings.In this study,the changing contexts for induced abortion in Francophone countries will be reviewed.Recent step undertaken by Benin to legalize abortion in most circumstances shows that Francophone countries may be overcoming their cultural barriers and adapting universal human rights based reproductive behaviors.
基金funded by the Key Project of the University Outstanding Youth Backbone Talented Person Foreign Visits Study Does Research of Anhui Province(No.gxgwfx2020025).
文摘Objective Heat wave exposure significantly impacts human health.Nevertheless,studies on the long-term effects of heat wave exposure during adolescence on adverse pregnancy outcomes(APOs)are rare.This study aimed to investigate the relationship between the long-term effects of heat wave exposure during adolescence and APOs.Methods We analyzed data from 3,376 female and 3,013 male participants across 31 provinces in China.All adolescents(10–19 years old),early adolescents(10–14),and late adolescents(15–19)were chosen as exposure windows.Heat waves were defined as periods lasting 2‒4 consecutive days with the daily temperature exceeding the 75th,90th,and 92.5th percentiles.We employed multivariate logistic regression models to assess the associations between exposure to heat waves during adolescence and APOs.Results The results revealed significant associations between male exposure to heat wave events during late adolescence and spontaneous abortion(P<0.05),which was more pronounced in South China.In contrast,no statistically significant associations were detected between males’exposure to heat wave events during adolescence and their partners’preterm birth(P>0.05 for all comparisons).The exposure of females to heat waves during adolescence was not significantly associated with subsequent spontaneous abortion or preterm birth(P>0.05 for all comparisons).Conclusions This study demonstrates that spontaneous abortion in females is associated with heat wave exposure in their male partner during adolescence.
文摘Background: Unsafe abortion (USA) is defined as the termination of a non-desired pregnancy, performed by an unqualified person or in an environment without minimum medical standards, or both. It can impact negatively the fertility in the future by tubo-peritoneal lesions. The current study aimed to highlight the infertility factors associated with USA. Material and Methods: From January 1st 2000 to December 31st 2021, a total of 3876 patients that sought care for infertility in the University Clinic of Kinshasa and the Edith Medical Center were enrolled. The socio-demographic, clinical and paraclinical variables were recorded and analyzed in relation to USA history. Results: The patients’ average age was 33.4 ± 5.7 years. Half of them were housewives;about sixty percent of the patients were nulliparous (59.31%). Seventy percent had a secondary infertility with 43.33% having a history of unsafe abortion. The main diagnosis was genital infections and uterus myoma. The chronic endometritis, bilateral tubal occlusion and Uterine synechiae were more associated with USA group. The spermogram anomalies and other partners’ lesions seemed of infectious origin. Conclusion: The USA remains a more frequent phenomenon in our setting. The chronic endometritis, bilateral tubal occlusion, uterine synechiae and indirectly uterus myoma were the infertility factors related to USA. The genital infection of any origin seems to be the main denominator underlying the female and the male infertility in our setting.
基金Supported by National Natural Science Foundation of China(31270674)Science and Technology Planning Project of Zhaoqing City,Guangdong Province(2019N012)National Undergraduate Innovation and Entrepreneurship Training Program(202210580007).
文摘[Objectives]This study was conducted to investigate the regulatory effects of selenium(Se)on the content and balance of endogenous hormones and the function of antioxidant system during seed development in Red sandalwood(Pterocarpus santalinus).[Methods]Two basic treatments,seven single-fertilization treatments,and four combined fertilization treatments were designed.Sampling was conducted at 2,5,8,and 18 weeks after flower withering to measure the embryo abortion percentage(EAP),the contents of three endogenous hormones(IAA,GA_(3),ABA),and the activities of four antioxidant enzymes(CAT,APX,SOD,GR).[Results]Se application significantly inhibited embryo abortion in Red sandalwood,with Na_(2)SeO_(3)[Se(IV)]showing superior effects to Na_(2)SeO_(3)(Se(VI))and far exceeding the efficacy of individual applications of KCl,H_(3)BO_(3),CO(NH_(2))_(2),Ca(H_(2)PO_(4))_(2),NPK compound fertilizer,or EFOF.The combined treatment of Se with NPK compound fertilizer and EFOF[EFOF+NPK compound fertilizer+Se(IV)]was the most effective,reducing the abortion percentage by 77.8%compared with UMC at 18 weeks after flower withering.Se application significantly increased the levels of three endogenous hormones and the(IAA+GA_(3))/ABA ratio in Red sandalwood seeds(including the embryonic stage).In the optimal treatment,the(IAA+GA_(3))content was 240.7%,256.4%,353.7%,and 502.9%higher than that of UMC at 2,5,8,and 18 weeks after flower withering,respectively.Se application also concurrently enhanced antioxidant enzyme activities,with all four antioxidant enzymes in seeds of Se-treated plants showing significant increases.Notably,the selenoenzyme GR maintained considerably high activity even at 18 weeks after flower withering.The EAP was highly significantly negatively correlated with IAA content and GR activity,identifying IAA and(IAA+GA_(3))content as key hormonal indicators and GR as the core antioxidant enzyme,together constituting the central regulatory factors.The results indicate that Se suppresses embryo abortion in Red sandalwood through a dual regulatory pathway:by elevating IAA and GA_(3)levels along with the(IAA+GA_(3))/ABA ratio to optimize hormonal signaling networks,and by enhancing the activities of antioxidant enzymes such as GR to alleviate oxidative stress induced by cool-season low temperatures.[Conclusions]This study provides a theoretical basis and technical strategy for precision fertilization and stress resistance management in the cultivation of Red sandalwood.
基金funded by National Health Mission,Government of West Bengal[HFW-35099/37/2018-SFWB SEC(DHS)(HFW)/4441,dated 23 August 2018].
文摘Objective:To assess comprehensive abortion care(CAC)services uptake and barriers to it in selected representative units of the three tier health care in the district of Malda in West Bengal,India.Methods:We conducted community-based,concurrent,parallel mixed methods study.Quantitative assessment of knowledge of primary health care workers on CAC and appropriate infrastructure assessment was done.Qualitative methods were used to identify themes affecting uptake of quality care among stakeholders.Results:A total of 48 staff nurses and 133 auxiliary nurses and midwives were included in the study.About half of nurses(of total 48)who were second tier health care provider and two thirds(of total 133)of first tier health care provider had incorrect knowledge about legality of abortion.Only 40%mothers seeking abortion were accompanied to subcentre by primary health care worker.At the third tier health care site,physicians insisted on necessity of refresher training.Suboptimal uptake of available CAC services was due to lack of enabling environment.Lack of updated training across health care workers was observed.Conclusions:Health care providers at the community level were unaware of facilities available for mothers seeking abortion.Higher level health care providers were unsure of specific knowledge about legal permissibility and sterilization of apparatus for abortion.At the highest level,more training on medical methods of abortion was required.The problems identified are amenable to correction,and some administrative and service-related provision on appropriate measures may lead to immediate success.
基金Supported by the Great Special Science and Technology Project of Hubei Province during the Eleventh Five-Year Plan(2006AA201A03)~~
文摘The Chaling wild abortive type （CL-type） cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical abortive pollens, and showed the characteristics of good outcrossing with a stigma exsertion rate of 48.2% and an outcrossing rate varied from 30% to 50%, good combining ability, and fine grain quality. E-Jing A was sensitive to temperature. Its duration from sowing to heading was 78-90 d when it was sown from mid May to early June at Wuhan. The breeding procedure of E-Jing A and its characteristics, relationship of restoring and maintaining were introduced.
