Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflam...Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflammatory,antioxidant,and antibacterial conditions.Methods:In this experimental study,extraction,preliminary phytochemical,gas chromatography-mass spectrometry(GC-MS)analysis,and safety profile of the alkaloid-rich fraction of Millettia aboensis(ARFMA)were carried out using standard biochemical methods.The female Wistar rats were given graded dose 100 and 200 mg/kg body weight(bw)of ARFMA leaves which serves as group 3 and 4 respectively.Group 2 rats received 100 mg/kg bw of misoprostol,while group 1 received 5 mL/kg bw as control.Results:The preliminary phytochemical result reveals significant amount of secondary metabolite such as alkaloids,flavonoids and terpenoids.In the GC-MS analysis,total of 13 compounds were detected and identified mainly as aromatic compounds.The ARFMA leaves showed antifertility at 50 and 25%.Meanwhile,200 mg/kg body weight of the ARFMA leaves resulted in 75%abortifacient action.The failure of embryo development is indicated by the ARFMA-treatment,which led to an increase in the percentage of resorption index.When compared to the control(P=0.05),serum progesterone levels significantly(P=0.04,P=0.02 and P=0.01)decreased in anti-implantation as a result of ARFMA-treatment for groups 2,3 and 4 respectively.In comparison to the control,the administration of ARFMA caused as an increase in serum estradiol.The histological architecture of the ovary and uterus disclosed that anti-implantation and abortifacient are consistent with the normal ovarian and uteri histology in the metestrus/diestrus stages of the oestrus cycle in ARFMA-treated groups,except changes which was observed in the uterus of abortifacient histological architecture.Conclusion:The ARFMA exhibits anti-implantation and abortifacient effects on the rats studied,causing a considerable decrease in the number of implants as well as resorption of developed foetuses.展开更多
文摘Background:There is a paucity of information on antifertility activity of Millettia aboensis,though it has been claimed to have a variety of medical qualities,including the treatment of ulcers,constipation,anti-inflammatory,antioxidant,and antibacterial conditions.Methods:In this experimental study,extraction,preliminary phytochemical,gas chromatography-mass spectrometry(GC-MS)analysis,and safety profile of the alkaloid-rich fraction of Millettia aboensis(ARFMA)were carried out using standard biochemical methods.The female Wistar rats were given graded dose 100 and 200 mg/kg body weight(bw)of ARFMA leaves which serves as group 3 and 4 respectively.Group 2 rats received 100 mg/kg bw of misoprostol,while group 1 received 5 mL/kg bw as control.Results:The preliminary phytochemical result reveals significant amount of secondary metabolite such as alkaloids,flavonoids and terpenoids.In the GC-MS analysis,total of 13 compounds were detected and identified mainly as aromatic compounds.The ARFMA leaves showed antifertility at 50 and 25%.Meanwhile,200 mg/kg body weight of the ARFMA leaves resulted in 75%abortifacient action.The failure of embryo development is indicated by the ARFMA-treatment,which led to an increase in the percentage of resorption index.When compared to the control(P=0.05),serum progesterone levels significantly(P=0.04,P=0.02 and P=0.01)decreased in anti-implantation as a result of ARFMA-treatment for groups 2,3 and 4 respectively.In comparison to the control,the administration of ARFMA caused as an increase in serum estradiol.The histological architecture of the ovary and uterus disclosed that anti-implantation and abortifacient are consistent with the normal ovarian and uteri histology in the metestrus/diestrus stages of the oestrus cycle in ARFMA-treated groups,except changes which was observed in the uterus of abortifacient histological architecture.Conclusion:The ARFMA exhibits anti-implantation and abortifacient effects on the rats studied,causing a considerable decrease in the number of implants as well as resorption of developed foetuses.
