Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approach...Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approaches,although minimally invasive,are often limited by insufficient tissue penetration,uncontrolled thermal damage,and narrow therapeutic windows.We introduce GHz high-repetition-rate pulsed lasers as a transformative modality for tumor ablation.This approach capitalizes on the thermal accumulation effect of GHz pulse trains,in which the pulse interval is significantly shorter than the thermal relaxation time of biological tissue.Such a regime enables efficient and localized heat deposition in tumor regions.By precisely tuning the repetition frequency,pulse duration,and energy density,we establish a dynamic“ablation-cooling”cycle:rapid energy delivery followed by transient inter-pulse cooling.This thermal modulation ensures sharply confined ablation zones with reduced collateral damage.Our systematic investigation of laser-tissue interaction parameters demonstrates that GHz lasers offer superior spatial selectivity,minimized off-target injury,and enhanced treatment safety,presenting a compelling rationale for clinical translation of this paradigm in precision photothermal oncology.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2022YFB3207204)the National Natural Science Foundation of China(Grant No.52293422)+6 种基金the Basic and Applied Basic Research Foundation of Guangdong Province-Regional Joint Fund-Key Projects(Grant Nos.2022B1515120012 and 2023B0101200003)the Department of Science and Technology of Guangdong Province(Grant No.2023B0101200003)the Science and Technology Innovation Commission of Shenzhen(Grant Nos.JCYJ20240813141317023,KJZD20240903095707010,KCXFZ20230731093259009,JCYJ20220818102618040,GJHZ20220913143207014,JCYJ20241202130558075,and KJZD20230923114002005)the Shenzhen Medical Research Fund(Grant Nos.D2301014 and D2402002)the Chemical Department of Hangzhou Normal University and the Ministry of Education Key Laboratory Open Scientific Projects Fund(Grant No.KFJJ2023007)the Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University,the Research Team Cultivation Program of Shenzhen University(Grant No.2023QNT008)the Graduate Independent Innovation Achievement Cultivation Project of Shenzhen University in 2025(Grant No.315-000066010715).
文摘Achieving precise tumor ablation without damaging surrounding healthy tissue remains a significant challenge in cancer therapy,particularly for deep-seated or irregularly shaped tumors.Traditional laser-based approaches,although minimally invasive,are often limited by insufficient tissue penetration,uncontrolled thermal damage,and narrow therapeutic windows.We introduce GHz high-repetition-rate pulsed lasers as a transformative modality for tumor ablation.This approach capitalizes on the thermal accumulation effect of GHz pulse trains,in which the pulse interval is significantly shorter than the thermal relaxation time of biological tissue.Such a regime enables efficient and localized heat deposition in tumor regions.By precisely tuning the repetition frequency,pulse duration,and energy density,we establish a dynamic“ablation-cooling”cycle:rapid energy delivery followed by transient inter-pulse cooling.This thermal modulation ensures sharply confined ablation zones with reduced collateral damage.Our systematic investigation of laser-tissue interaction parameters demonstrates that GHz lasers offer superior spatial selectivity,minimized off-target injury,and enhanced treatment safety,presenting a compelling rationale for clinical translation of this paradigm in precision photothermal oncology.
