AP2/EREBP(APETALA2/ethylene-responsive element binding proteins)是一个起源古老的转录因子超家族,它含有1个或2个由约60-70个氨基酸残基组成的非常保守的DNA结合域(DNA-bindingdomain),即AP2/ERF结构域。根据AP2/ERF结构域的数目,A...AP2/EREBP(APETALA2/ethylene-responsive element binding proteins)是一个起源古老的转录因子超家族,它含有1个或2个由约60-70个氨基酸残基组成的非常保守的DNA结合域(DNA-bindingdomain),即AP2/ERF结构域。根据AP2/ERF结构域的数目,AP2/EREBP转录因子可以分为2个亚族:EREBP亚族(具有1个AP2/ERF结构域)和AP2亚族(具有2个AP2/ERF结构域)。AP2亚族转录因子有调控花、胚珠和种子发育的功能,而EREBP亚族转录因子(包括DREB类和ERF类)的主要功能是调节植物对激素(乙烯和ABA等)、病原和胁迫(低温、干旱及高盐)等的应答反应。本文讨论了AP2/EREBP转录因子在植物发育和胁迫应答中的研究进展。展开更多
Acer paxii belongs to the evergreen species of Acer,but it exhibits a unique feature of reddish leaves in fall in subtropical regions.Although the association of AP2/ERF transcription factors with color change has bee...Acer paxii belongs to the evergreen species of Acer,but it exhibits a unique feature of reddish leaves in fall in subtropical regions.Although the association of AP2/ERF transcription factors with color change has been well-documented in prior research,molecular investigations focusing on AP2/ERF remain notably lacking in Acer paxii.This research focuses on performing an extensive genome-wide investigation to identify and characterize the AP2/ERF gene family in Acer paxii.As a result,123 ApAP2/ERFs were obtained.Phylogenetic analyses categorized the ApAP2/ERF family members into 15 subfamilies.The evolutionary traits of the ApAP2/ERFs were investigated by analyzing their chromosomal locations,conserved proteinmotifs,and gene duplication events.Moreover,investigating gene promoters revealed their potential involvement in developmental regulation,physiological processes,and stress adaptationmechanisms.Measurements of anthocyanin content revealed a notable increase in red leaves during autumn.Utilizing transcriptome data,transcriptomic profiling revealed that the majority of AP2/ERF genes in Acer paxii displayed significant differential expression between red and green leaves during the color-changing period.Furthermore,through qRT-PCR analysis,it was found that the gene expression levels of ApERF006,ApERF014,ApERF048,ApERF097,and ApERF107 were significantly elevated in red leaves.This indicates their potential participation in leaf pigmentation processes.These findings offer significant insights into the biological significance of ApAP2/ERF transcription factors and lay the groundwork for subsequent investigations into their regulatorymechanisms underlying leaf pigmentation in Acer paxii.展开更多
文摘AP2/EREBP(APETALA2/ethylene-responsive element binding proteins)是一个起源古老的转录因子超家族,它含有1个或2个由约60-70个氨基酸残基组成的非常保守的DNA结合域(DNA-bindingdomain),即AP2/ERF结构域。根据AP2/ERF结构域的数目,AP2/EREBP转录因子可以分为2个亚族:EREBP亚族(具有1个AP2/ERF结构域)和AP2亚族(具有2个AP2/ERF结构域)。AP2亚族转录因子有调控花、胚珠和种子发育的功能,而EREBP亚族转录因子(包括DREB类和ERF类)的主要功能是调节植物对激素(乙烯和ABA等)、病原和胁迫(低温、干旱及高盐)等的应答反应。本文讨论了AP2/EREBP转录因子在植物发育和胁迫应答中的研究进展。
基金supported by the National Natural Science Foundation of China[grant numbers 32271914 and 32301660]the Quality Engineering Project of Anhui Provincial Department of Education[grant number 2023zygzts007].
文摘Acer paxii belongs to the evergreen species of Acer,but it exhibits a unique feature of reddish leaves in fall in subtropical regions.Although the association of AP2/ERF transcription factors with color change has been well-documented in prior research,molecular investigations focusing on AP2/ERF remain notably lacking in Acer paxii.This research focuses on performing an extensive genome-wide investigation to identify and characterize the AP2/ERF gene family in Acer paxii.As a result,123 ApAP2/ERFs were obtained.Phylogenetic analyses categorized the ApAP2/ERF family members into 15 subfamilies.The evolutionary traits of the ApAP2/ERFs were investigated by analyzing their chromosomal locations,conserved proteinmotifs,and gene duplication events.Moreover,investigating gene promoters revealed their potential involvement in developmental regulation,physiological processes,and stress adaptationmechanisms.Measurements of anthocyanin content revealed a notable increase in red leaves during autumn.Utilizing transcriptome data,transcriptomic profiling revealed that the majority of AP2/ERF genes in Acer paxii displayed significant differential expression between red and green leaves during the color-changing period.Furthermore,through qRT-PCR analysis,it was found that the gene expression levels of ApERF006,ApERF014,ApERF048,ApERF097,and ApERF107 were significantly elevated in red leaves.This indicates their potential participation in leaf pigmentation processes.These findings offer significant insights into the biological significance of ApAP2/ERF transcription factors and lay the groundwork for subsequent investigations into their regulatorymechanisms underlying leaf pigmentation in Acer paxii.
