In this paper, multiferroics and magnetocapacitive effect of triangular-lattice antiferromagnet Ag Al0.02Cr0.98S2 are investigated by magnetic, ferroelectric, pyroelectric current and dielectric measurement. We find t...In this paper, multiferroics and magnetocapacitive effect of triangular-lattice antiferromagnet Ag Al0.02Cr0.98S2 are investigated by magnetic, ferroelectric, pyroelectric current and dielectric measurement. We find that it is a multiferroic material and the magnetocapacitive effect reaches a factor of up to 90 in an external field of 7 T. The results imply the further possibility of synthesizing the magnetocapacitive materials by modifying the frustrated spin structure in terms of a few B-site doping nonmagnetic ions.展开更多
Chlorella sp.is able to grow and transform inorganic and organic contaminants in wastewater to create biomass.In the present study,Chlorella sp.LH2 isolated from cocoon wastewater was able to thrive in hospital wastew...Chlorella sp.is able to grow and transform inorganic and organic contaminants in wastewater to create biomass.In the present study,Chlorella sp.LH2 isolated from cocoon wastewater was able to thrive in hospital wastewater,then remove nutrients and eliminate E.coli ATCC 8739.The results indicated that optimal cultivation conditions of Chlorella sp.LH2 in hospital wastewater were pH of 8,light:dark cycle of 16:8 at 30℃.The inhibitory effect of chlorination on algae growth was accompanied with the chlorine concentration.BOD_(5):COD ratio of 0.77 indicated biodegradability of hospital wastewater.The untreated and treated wastewatee samples were collected to investigated the nutrient removal efficiency after 10 days.Untreated and treated results were192±8.62 mg/l 23.91±2.19 mg/l for BOD_(5);245±9.15 mg/l and 47.31±5.71 mg/l for COD.The treated value met the required standards for hospital wastewater treatment.The removal efficiency total nitrogen and total phosphorus were 68.64%and 64.44%after 10 days,respectively.Elimination of E.coli ATCC 8739 after 7 days by Chlorella sp.LH2 was 88.92%.The results of this study suggest the nutrients and pathogens removal potential of Chlorella sp.LH2 in hospital wastewater for further practical applications.展开更多
The xenogeneic decellularized corneal matrix(DCM)was expected to be used in lamellar keratoplasty in clinic as the substitute of allogeneic cornea.After decellularization treatment,the remaining risk of xenograft reje...The xenogeneic decellularized corneal matrix(DCM)was expected to be used in lamellar keratoplasty in clinic as the substitute of allogeneic cornea.After decellularization treatment,the remaining risk of xenograft rejection needed to be assessed.The galactose-α1,3-galactose,as the most abundant and closely rejection-related xenogeneic antigen,should be one of the important factors concerned in immunological evaluation.In this study,residual αGal in the DCM was first determined by an enzyme-linked immunosorbent assay method with qualified accuracy and specificity.Then the DCM was implanted subcutaneously into the α1,3-galactosyltransferase gene-knockout(GTKO)mice,accompanied by the implantation in the wild-type C57BL/6 mice as a comparison.The total serum antibody levels,anti-Gal antibody levels,inflammatory cytokines and ratios of splenic lymphocyte subtypes were detected and the histopathological analysis of implants were performed to systematically evaluate the immune responses.The experimental result showed the fresh porcine corneal matrix samples had(9.90±1.54)×10^12 αGal epitope per mg while the content of residual aGal in the DCM was(7.90±2.00)×10^12 epitope per mg.The GTKO mice had similar potential of reaction to immune stimulation to that of wild-type C57BL/6 mice.At 4 weeks after implantation of DCM,in WT mice and GTKO mice there were both innate immunity response to the DCM characterized by macrophage infiltration.But the elevations of anti-Gal IgG level and the percentage of splenic natural killer cells were only detected in GTKO mice.These changes were thought to be pertinent to the residual αGal antigen,which could not be detected in WT mice.No further αGal antibody-mediated cellular immunity and significant changes of serum cytokine contents were found in GTKO mice,which perhaps suggested that the immune reactions to the DCM after 4 weeks of implantation were moderate and had minor effect on the survival of the corneal graft.展开更多
Hepatocellular carcinoma(HCC)is a common malignant tumor with poor prognosis and high mortality.In this study,we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant...Hepatocellular carcinoma(HCC)is a common malignant tumor with poor prognosis and high mortality.In this study,we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant MHCC97H cells expressing porcine a-1,3-galactose epitopes(a Gal)and endorphin extracellular domains(END)with dendritic cells(DCs)from healthy volunteers.END^(+)/Gal^(+)-MHCC97H/DC fusion cells induced cytotoxic T lymphocytes(CTLs)and secretion of interferon-gamma(IFN-γ).CTLs targeted cells expressing a Gal and END and tumor angiogenesis.The fused cell vaccine can effectively inhibit tumor growth and prolong the survival time of human hepatoma mice,indicating the high clinical potential of this new cell based vaccine.展开更多
基金Project supported by the National Basic Research Program of China(Grant No.2010CB833102)the National Natural Science Foundation of China(Grant Nos.10974244+1 种基金11274369and 11104337)
文摘In this paper, multiferroics and magnetocapacitive effect of triangular-lattice antiferromagnet Ag Al0.02Cr0.98S2 are investigated by magnetic, ferroelectric, pyroelectric current and dielectric measurement. We find that it is a multiferroic material and the magnetocapacitive effect reaches a factor of up to 90 in an external field of 7 T. The results imply the further possibility of synthesizing the magnetocapacitive materials by modifying the frustrated spin structure in terms of a few B-site doping nonmagnetic ions.
基金Part of this research funding is supported by the Vietnamese Ministry of Education&Training,Vietnam with a number named B2021-DLA-01.
文摘Chlorella sp.is able to grow and transform inorganic and organic contaminants in wastewater to create biomass.In the present study,Chlorella sp.LH2 isolated from cocoon wastewater was able to thrive in hospital wastewater,then remove nutrients and eliminate E.coli ATCC 8739.The results indicated that optimal cultivation conditions of Chlorella sp.LH2 in hospital wastewater were pH of 8,light:dark cycle of 16:8 at 30℃.The inhibitory effect of chlorination on algae growth was accompanied with the chlorine concentration.BOD_(5):COD ratio of 0.77 indicated biodegradability of hospital wastewater.The untreated and treated wastewatee samples were collected to investigated the nutrient removal efficiency after 10 days.Untreated and treated results were192±8.62 mg/l 23.91±2.19 mg/l for BOD_(5);245±9.15 mg/l and 47.31±5.71 mg/l for COD.The treated value met the required standards for hospital wastewater treatment.The removal efficiency total nitrogen and total phosphorus were 68.64%and 64.44%after 10 days,respectively.Elimination of E.coli ATCC 8739 after 7 days by Chlorella sp.LH2 was 88.92%.The results of this study suggest the nutrients and pathogens removal potential of Chlorella sp.LH2 in hospital wastewater for further practical applications.
基金supported by the funds from National Key Research and Development Program of Ministry of science and technology of China(2016YFC1103200 and 2016YFC1103203).
文摘The xenogeneic decellularized corneal matrix(DCM)was expected to be used in lamellar keratoplasty in clinic as the substitute of allogeneic cornea.After decellularization treatment,the remaining risk of xenograft rejection needed to be assessed.The galactose-α1,3-galactose,as the most abundant and closely rejection-related xenogeneic antigen,should be one of the important factors concerned in immunological evaluation.In this study,residual αGal in the DCM was first determined by an enzyme-linked immunosorbent assay method with qualified accuracy and specificity.Then the DCM was implanted subcutaneously into the α1,3-galactosyltransferase gene-knockout(GTKO)mice,accompanied by the implantation in the wild-type C57BL/6 mice as a comparison.The total serum antibody levels,anti-Gal antibody levels,inflammatory cytokines and ratios of splenic lymphocyte subtypes were detected and the histopathological analysis of implants were performed to systematically evaluate the immune responses.The experimental result showed the fresh porcine corneal matrix samples had(9.90±1.54)×10^12 αGal epitope per mg while the content of residual aGal in the DCM was(7.90±2.00)×10^12 epitope per mg.The GTKO mice had similar potential of reaction to immune stimulation to that of wild-type C57BL/6 mice.At 4 weeks after implantation of DCM,in WT mice and GTKO mice there were both innate immunity response to the DCM characterized by macrophage infiltration.But the elevations of anti-Gal IgG level and the percentage of splenic natural killer cells were only detected in GTKO mice.These changes were thought to be pertinent to the residual αGal antigen,which could not be detected in WT mice.No further αGal antibody-mediated cellular immunity and significant changes of serum cytokine contents were found in GTKO mice,which perhaps suggested that the immune reactions to the DCM after 4 weeks of implantation were moderate and had minor effect on the survival of the corneal graft.
基金supported,in part,by grants from the State Project for Essential Drug Research and Development(No.2019ZX09301132,China)National Natural Science Foundation of China(Nos.82060562 and 82072340)+2 种基金the Scientific and Technological Innovation Major Base of Guangxi(No.2018-15Z04,China)Guangxi Key Research and Development Project(No.AB20117001,China)Guangxi Natural Science Foundation(No.2018JJA140524,China)。
文摘Hepatocellular carcinoma(HCC)is a common malignant tumor with poor prognosis and high mortality.In this study,we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant MHCC97H cells expressing porcine a-1,3-galactose epitopes(a Gal)and endorphin extracellular domains(END)with dendritic cells(DCs)from healthy volunteers.END^(+)/Gal^(+)-MHCC97H/DC fusion cells induced cytotoxic T lymphocytes(CTLs)and secretion of interferon-gamma(IFN-γ).CTLs targeted cells expressing a Gal and END and tumor angiogenesis.The fused cell vaccine can effectively inhibit tumor growth and prolong the survival time of human hepatoma mice,indicating the high clinical potential of this new cell based vaccine.
