The application of gene delivery materials has been mainly focused on mammalian cells while rarely extended to plant engineering. Cationic polymers and lipids have been widely utilized to efficiently deliver DNA and s...The application of gene delivery materials has been mainly focused on mammalian cells while rarely extended to plant engineering. Cationic polymers and lipids have been widely utilized to efficiently deliver DNA and siRNA into mammalian cells. However, their applica- tion in plant cells is limited due to the different membrane structures and the presence of plant cell walls. In this study, we developed the cationic, a-helical polypeptide that can effectively deliver DNA into both isolated Arabidopsis thaliana protoplasts and intact leaves. The PPABLG was able to condense DNA to form nanocomplexes, and they exhibited significantly improved transfection efficiencies compared with commercial transfection reagent Lipofec- tamine 2000 and classical cell penetrating peptides such as poly（L-lysine）, HIV-TAT, arginine9, and poly（L-arginine）. This study therefore widens the utilities of helical polypeptide as a unique category of gene delivery materials, and may find their promising applications toward plant gene delivery.展开更多
We mainly investigate the variable-coefficient 3-coupled nonlinear Schrodinger(NLS)system,which describes soli- ton dynamics in the three-spineα-helical protein with inhomogeneous effect.The variable-coefficient NLS ...We mainly investigate the variable-coefficient 3-coupled nonlinear Schrodinger(NLS)system,which describes soli- ton dynamics in the three-spineα-helical protein with inhomogeneous effect.The variable-coefficient NLS equation is transformed into the constant coefficient NLS equation by similarity transformation firstly.The Hirota method is used to solve the constant coefficient NLS equation,and then we get the one-and two-breather solutions of the variable-coefficient NLS equation.The results show that,in the background of plane waves and periodic waves,the breather can be transformed into some forms of combined soliton solutions.The influence of different parameters on the soliton solution and the collision between two solitons are discussed by some graphs in detail.Our results are helpful to study the soliton dynamics inα-helical protein.展开更多
Using targeted toxins is a promising approach for the therapy of cancer and autoimmune diseases, as well as other disorders/The high mobility group chromosomal protein N2 （HMGN2） is one of the most abundant and well...Using targeted toxins is a promising approach for the therapy of cancer and autoimmune diseases, as well as other disorders/The high mobility group chromosomal protein N2 （HMGN2） is one of the most abundant and well-characterized classes of nonhistone nuclear proteins, which seems to function as architectural elements in chromatin.2 Recently our group isolated an antimicrobial polypeptide from human LAK cells and cervical mucus,展开更多
文摘The application of gene delivery materials has been mainly focused on mammalian cells while rarely extended to plant engineering. Cationic polymers and lipids have been widely utilized to efficiently deliver DNA and siRNA into mammalian cells. However, their applica- tion in plant cells is limited due to the different membrane structures and the presence of plant cell walls. In this study, we developed the cationic, a-helical polypeptide that can effectively deliver DNA into both isolated Arabidopsis thaliana protoplasts and intact leaves. The PPABLG was able to condense DNA to form nanocomplexes, and they exhibited significantly improved transfection efficiencies compared with commercial transfection reagent Lipofec- tamine 2000 and classical cell penetrating peptides such as poly（L-lysine）, HIV-TAT, arginine9, and poly（L-arginine）. This study therefore widens the utilities of helical polypeptide as a unique category of gene delivery materials, and may find their promising applications toward plant gene delivery.
基金supported by the Scientific and Technological Innovation Programs of Higher Education Institution in Shanxi,China(Grant Nos.2020L0525 and 2019L0782)the National Natural Science Foundation of China(Grant Nos.11805141 and 12075210)+2 种基金Applied Basic Research Program of Shanxi Province,China(Grant No.201901D211424)“1331 Project”Key Innovative Research Team of Taiyuan Normal University(Grant No.I0190364)Key Research and Development program of Shanxi Province,China(Grant No.201903D421042).
文摘We mainly investigate the variable-coefficient 3-coupled nonlinear Schrodinger(NLS)system,which describes soli- ton dynamics in the three-spineα-helical protein with inhomogeneous effect.The variable-coefficient NLS equation is transformed into the constant coefficient NLS equation by similarity transformation firstly.The Hirota method is used to solve the constant coefficient NLS equation,and then we get the one-and two-breather solutions of the variable-coefficient NLS equation.The results show that,in the background of plane waves and periodic waves,the breather can be transformed into some forms of combined soliton solutions.The influence of different parameters on the soliton solution and the collision between two solitons are discussed by some graphs in detail.Our results are helpful to study the soliton dynamics inα-helical protein.
基金This study was supported by grants from the China Methcal Board of New York Inc. （No. 98-681）, and the National Natural Science Foundation of China （No. 30470763 and No. 30671963）.
文摘Using targeted toxins is a promising approach for the therapy of cancer and autoimmune diseases, as well as other disorders/The high mobility group chromosomal protein N2 （HMGN2） is one of the most abundant and well-characterized classes of nonhistone nuclear proteins, which seems to function as architectural elements in chromatin.2 Recently our group isolated an antimicrobial polypeptide from human LAK cells and cervical mucus,
