Aphid-borne Zucchini yellow mosaic virus （ZYMV） is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection techniq...Aphid-borne Zucchini yellow mosaic virus （ZYMV） is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybridoma cell lines （16A11, 5A7 and 3B8） secreting monoclonal antibodies （MAbs） against ZYMV Zhejiang isolate were obtained. The working titers of the ascitic fluids secreted by the three hybridoma cell lines were up to 10^-7 by indirect enzyme-linked immunosorbent assay （ELISA）. All MAbs were isotyped as IgG1, kappa light chain. Western blot analysis indicated that the MAb 3B8 could specifically react with the coat protein of ZYMV while MAbs 5A7 and 16A11 reacted strongly with a protein of approximately 51 kDa from the ZYMV-infected leaf tissues. According to this molecular weight, we consider this reactive protein As likely to be the HC-Pro protein. Using these three MAbs, we have now developed five detection assays, i.e., antigen-coated-plate ELISA （ACP-ELISA）, dot-ELISA, tissue blot-ELISA, double-antibody sandwich ELISA （DAS-ELISA）, and immunocapture-RT-PCR （IC-RT-PCR）, for the sensitive, specific, and easy detection of ZYMV. The sensitivity test revealed that ZYMV could be readily detected respectively by ACP-ELISA, dot-ELISA, DAS-ELISA and IC-RT-PCR in 1：163840, 1：2560, 1：327680 and 1：1 310720 （w/v, g mL-1） diluted crude extracts from the ZYMV-infected plants. We demonstrated in this study that the dot-ELISA could also be used to detect ZYMV in individual viruliferous aphids. A total of 275 cucurbitaceous plant samples collected from the Zhejiang, Jiangsu, Shandong and Hainan provinces, China, were screened for the presence of ZYMV with the described assays. Our results showed that 163 of the 275 samples （59%） were infected with ZYMV. This finding indicates that ZYMV As now widely present in cucurbitaceous crops in China. RT-PCR followed by DNA sequencing and sequence analyses confirmed the accuracy of the five assays. We consider that these detection assays can significantly benefit the control of ZYMV in China.展开更多
In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication o...In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.展开更多
Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So...Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So, it is important to develop specific gene markers to allow rapid diagnosis by PCR. Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for Tomato yellow leaf curl virus (TYLCV) by the resistance gene Ty-1, in zucchini for Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus estirpe watermelon (PRSV-W), and in lettuce for Lettuce mosaic virus (LMV). Fragments of 249 bp were amplified only by resistant plants to TYLCV as the hybrids 2648 and Aguamiel, and not for varieties as Santa Cruz or Carina. It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle;for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant;for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Babá de Verão. Finally, it was observed that primers PK47F/R were able to check the Cabotiá seed hybrids of pumpkin Jabras.展开更多
[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus ...[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.展开更多
The effects of fresh zucchini on nutritional status, and biological indicators for the prevention of cardiovascular disease in rats fed high-fat diets investigated in this study. Thirty Sprague Dawley rats were random...The effects of fresh zucchini on nutritional status, and biological indicators for the prevention of cardiovascular disease in rats fed high-fat diets investigated in this study. Thirty Sprague Dawley rats were randomly divided into two main groups the first, negative control group Co (-) (n = 6), fed basal diet, The second group (n = 24) fed high-fat diet (containing basal diet + 5% tallow + 1% cholesterol + 0.02% bile salt). This group was divided into four subgroups each group 6 rats: group positive control co (+) fed high-fat diet only, group 1 (G (1)) fed high-fat diet plus 10% zucchini, group 2 (G (2) fed high-fat diet plus 15% zucchini and group 3 (G (3)) fed high-fat diet plus 20% zucchini. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured after eight wk. of experimental treatment. The pathologic changes of the heart, spleen and kidney were evaluated. SPSS, one way ANOVA was used to analyze the results. The results indicated that the mean values of body weight gain (BWG) and feed efficiency ratio (FIR) in G (2) and G (3) showed significant decrease compared to co (-), co (+) and G 1. The results showed that in groups (G1, G2 and G3) the relative weight of heart had significant increase when compared with control negative group. But liver and spleen weight had no significant difference when compared with control negative and positive group, the levels of TC, TG and LDL-C were significantly increased in co (+) (P < 0.05) Compared to co (-), all groups fed on high fat diet containing different levels of zucchini (10%, 15% and 20%) had significant decrease in TC and LDL-C compared with co (+), also G2 and G3 had improve significant in HDL-C when compared with co. (+). The groups fed on zucchini give results similar to group co. (-). The best treatment was zucchini (15% and 20%) which had lowest values of total lipid cholesterol and LDL-C, and the best values of HDL-C, HDL-C/TC % for all groups fed on high fat diet and zucchini increased non significantly (p ≤ 0.05) the HDL-C/TC % index compared to group co. (+). while, G2 and G3 gave significant decrease in LDL-C/HDL-C ratio compared to group co. (+). Morphologic changes of heart, spleen and kidney revealed that groups G2 and G3 had a similar preventive effect against CHD in this experimental model. In conclusion, results showed that zucchini had similar potential to attenuate CHD-related parameters in a mild oxidative stress induced by high-fat diet in rats.展开更多
Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-p...Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.展开更多
This paper elaborates the symptom characteristics and control methods of virus diseases including squash leaf curl china virus(SLCCNV),watermelon mosaic virus(WMV),cucurbit chlorotic yellows virus(CCYV),cucumber mosai...This paper elaborates the symptom characteristics and control methods of virus diseases including squash leaf curl china virus(SLCCNV),watermelon mosaic virus(WMV),cucurbit chlorotic yellows virus(CCYV),cucumber mosaic virus(CMV)and zucchini yellow mosaic virus(ZYMV),as well as fungal diseases including zucchini powdery mildew,zucchini downy mildew and zucchini scab that harm zucchini production,in order to provide a theoretical basis and technical support for disease prevention and control of zucchini.展开更多
The Zucchini yellow mosaic virus （ZYMV） （Potyvirus） on “zucchini” presents great economic importance for Malagasy farmers. Numerous aphid species （Hemiptera： Aphididae） spread viral particles, which are easil...The Zucchini yellow mosaic virus （ZYMV） （Potyvirus） on “zucchini” presents great economic importance for Malagasy farmers. Numerous aphid species （Hemiptera： Aphididae） spread viral particles, which are easily transmitted mechanically, too. Farmers ignored this disease, therefore, its control became extremely difficult with insecticides. This study aimed to evaluate efficacy of chili pepper extracts. Treatments vary as a function of its initiations and were repeated at weekly intervals until harvest. It was conducted in market gardens around the town of Antananarivo. The study compared four treatments and repeated three times： firstly, plots received a protection as soon as installation of culture （preventive protection）; secondly, plots received treatment with low infestation （about a quarter plants infested） （late protection）; thirdly, plots received treatment with high infestation （about half of plants infested） （latest protection）; lastly, plots without treatment （the control）. As a result, pepper extracts can provide a significant level to control against aphids attack, and permit to delay virus installation on plots, which received treatment as soon as implementation of culture. However, it cannot eradicate and cannot limit extend of virus disease on plots already infested. Economic analysis shows that preventive treatment of pepper extracts to fight virus attack provides an even greater return on its investment than all other terms. Thus, use of pepper extracts can reduce chemical treatment and pollution.展开更多
The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber （Cuc...The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber （Cucurnis sativus L.） and zucchini （Cucurbita pepo L.） plants were grown in closed-soilless culture under unheated-greenhouse conditions at the experimental farm of University of Tuscia, Central Italy to evaluate the effects of four substrates （rockwool, pumice, perlite, and cocofiber） on growth, yield and plant mineral composition. For both cultures, plants grown in cocofiber, perlite and pumice yielded more than those grown in rockwool. The better temperature regime in cocofiber, perlite and pumice was due to the greater thermal inertia compared to rockwool slabs. Thc concentration of N in zucchini and cucumber leaves was significantly higher in cocofiber, perlite and pumice in comparison to the rockwool treatment. The concentration of K was significantly affected by the substrate only for the zucchini squash with the highest value recorded on the organic substrate （cocofiber）, whereas the Ca concentration was significantly influenced by the growing media only for cucumber with the highest value observed on pumice. Finally, the lowest Mg concentration in leaf tissue was observed on plants grown with the rockwool substrate for both zucchini squash and cucumber. We can conclude that cocofiber, perlite and pumice are suitable for zucchini and cucumber production in closed soilless system, whereas the use of rockwool is more suitable for crops grown under heated greenhouse conditions.展开更多
[Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were...[Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were determined.[Results]The yield of Shengfeier,Xiuyu 170 and Xihulu 309 increased by 11.4%,6.9%and 4.6%,respectively compared with S68(control),and zucchini was straight,looked pleasing to the eye,and had strong disease resistance.[Conclusions]The zucchini varieties were selected and the high-quality,high-yield and high-efficiency cultivation techniques were integrated.展开更多
以3种西葫芦(“碧玉”西葫芦BZ、“金皮香蕉”西葫芦JZ和“墨玉”西葫芦MZ)为研究对象,探究其营养成分、生物活性物质及体外抗氧化和降血糖活性差异。结果显示,3种西葫芦水分含量高(>95%),粗脂肪含量(<0.105 g/100 g FW)和总能量(...以3种西葫芦(“碧玉”西葫芦BZ、“金皮香蕉”西葫芦JZ和“墨玉”西葫芦MZ)为研究对象,探究其营养成分、生物活性物质及体外抗氧化和降血糖活性差异。结果显示,3种西葫芦水分含量高(>95%),粗脂肪含量(<0.105 g/100 g FW)和总能量(<70.00 kJ/100 g FW)低,粗蛋白质含量适中(0.833~0.945 g/100 g FW),但含有较丰富的总碳水化合物和维生素C,其中BZ含量最高,达到3.08 g/100 g FW和12.04 mg AA/100 g FW;矿物质元素分析表明,3种西葫芦富含钾、磷和钙元素,且具有较高的钾钠比;氨基酸组成分析表明,3种西葫芦均含有多种氨基酸,且必需氨基酸占总氨基酸的34%以上;脂肪酸分析表明,BZ中含有更丰富的不饱和脂肪酸,占其脂肪酸总量的76.84%;BZ中总酚和总黄酮含量显著高于其他两个品种,并从BZ中共分离鉴定出11种酚类化合物,MZ西葫芦中共7种,JZ西葫芦中共5种,其中,BZ中的槲皮素-3,4'-二甲醚-7-O-芸香糖苷首次在西葫芦中鉴定发现。体外活性研究表明,BZ提取物具有更好的抗氧化和淀粉消化酶抑制活性。综上,西葫芦是一种营养丰富的低脂低能量蔬菜,但不同品种西葫芦的营养成分组成、含量和活性存在显著差异。展开更多
为明确0.5%香菇多糖水剂对西葫芦病毒病的田间预防效果和治疗效果以及对作物的安全性。采用随机区组试验的方式在田间对西葫芦病毒病进行试验。结果表明,各个处理未见药害症状,对植株安全。0.5%香菇多糖水剂在6.0~10 g a.i./hm^(2)用量...为明确0.5%香菇多糖水剂对西葫芦病毒病的田间预防效果和治疗效果以及对作物的安全性。采用随机区组试验的方式在田间对西葫芦病毒病进行试验。结果表明,各个处理未见药害症状,对植株安全。0.5%香菇多糖水剂在6.0~10 g a.i./hm^(2)用量下可有效防治西葫芦病毒病,第1次药后21 d发病前药剂处理相对防效可达52.25%~74.02%,发病初期药剂处理相对防效可达54.69%~61.40%,且病害发生前使用相较于病害发生后使用能延缓病害蔓延。因此,0.5%香菇多糖水剂在田间可以有效防治西葫芦病毒病,建议推荐用量为10 g a.i./hm^(2),且在病毒病常发地区,建议发病前进行药剂预防,可显著提高防治效果。展开更多
[Objectives]This study was conducted to detect and analyze tomato leaf curl New Delhi virus(ToLCNDV).[Methods]Through PCR detection,sequence analysis,and pathogenicity verification,tomato leaf curl New Delhi virus(ToL...[Objectives]This study was conducted to detect and analyze tomato leaf curl New Delhi virus(ToLCNDV).[Methods]Through PCR detection,sequence analysis,and pathogenicity verification,tomato leaf curl New Delhi virus(ToLCNDV)was identified in zucchini exhibiting systemic disease symptoms during a 2024 outbreak in Qingzhou City,Shandong Province,and was designated as ToLCNDV-SD.[Results]Specific primer amplification showed that all eight diseased samples produced bands of 504 bp(DNA-A)and 892 bp(DNA-B).Sequencing analysis revealed that ToLCNDV-SD DNA-A shared 96.10%homology with an Indonesian melon isolate(LC421834.1),while DNA-B showed 88.31%homology with a Malaysian bitter gourd isolate(MW248678.1).Phylogenetic analysis indicated its closest relationship with Southeast Asian cucurbit-infecting isolates.Friction transmission tests confirmed that the virus could spread mechanically,inducing typical symptoms 14 d after inoculation with positive PCR detection.[Conclusions]This study provides important insights for understanding the epidemic mechanisms and control strategies of ToLCNDV in China.展开更多
基金supported by the National Natural Science Foundation of China(31272015)the National Basic Research Program(973)of China(2014CB138400)the Special Fund for Agro-scientific Research in the Public Interest,China(201303021,201303028)
文摘Aphid-borne Zucchini yellow mosaic virus （ZYMV） is one of the most economically important viruses of cucurbitaceous plants. To survey and control this virus, it is necessary to develop an efficient detection technique. Using purified ZYMV virion and the conventional hybridoma technology, three hybridoma cell lines （16A11, 5A7 and 3B8） secreting monoclonal antibodies （MAbs） against ZYMV Zhejiang isolate were obtained. The working titers of the ascitic fluids secreted by the three hybridoma cell lines were up to 10^-7 by indirect enzyme-linked immunosorbent assay （ELISA）. All MAbs were isotyped as IgG1, kappa light chain. Western blot analysis indicated that the MAb 3B8 could specifically react with the coat protein of ZYMV while MAbs 5A7 and 16A11 reacted strongly with a protein of approximately 51 kDa from the ZYMV-infected leaf tissues. According to this molecular weight, we consider this reactive protein As likely to be the HC-Pro protein. Using these three MAbs, we have now developed five detection assays, i.e., antigen-coated-plate ELISA （ACP-ELISA）, dot-ELISA, tissue blot-ELISA, double-antibody sandwich ELISA （DAS-ELISA）, and immunocapture-RT-PCR （IC-RT-PCR）, for the sensitive, specific, and easy detection of ZYMV. The sensitivity test revealed that ZYMV could be readily detected respectively by ACP-ELISA, dot-ELISA, DAS-ELISA and IC-RT-PCR in 1：163840, 1：2560, 1：327680 and 1：1 310720 （w/v, g mL-1） diluted crude extracts from the ZYMV-infected plants. We demonstrated in this study that the dot-ELISA could also be used to detect ZYMV in individual viruliferous aphids. A total of 275 cucurbitaceous plant samples collected from the Zhejiang, Jiangsu, Shandong and Hainan provinces, China, were screened for the presence of ZYMV with the described assays. Our results showed that 163 of the 275 samples （59%） were infected with ZYMV. This finding indicates that ZYMV As now widely present in cucurbitaceous crops in China. RT-PCR followed by DNA sequencing and sequence analyses confirmed the accuracy of the five assays. We consider that these detection assays can significantly benefit the control of ZYMV in China.
文摘In Japan, zucchini culture has yet to get underway, and the current costs of zucchini can be attributed to damage from soil-borne disease and the unstable yields due to seasonal change of female flowers. Eradication of these problems will lead to stable supply and a consequent price reduction of zucchini fruits. We previously clarified the efficacy of potted culture as a new culture method for zucchini, but potted culture can be burdensome as the weak water and nutrient retention capacity of the medium warrants its regular replacement. To solve this problem, in this study, we investigated the blend ratio for mixing rice husk charcoal with peat and the amount of fertilizer required for potted culture of zucchini. Results revealed no significant differences in the length of the largest leaf, total number of flowers, number of female flowers, and the ratio of female flowers to total flowers with different blend ratios of rice husk charcoal to peat. However, the number of harvested fruits increased with higher ratios of rice husk charcoal to peat and was highest at 80:20. The length of the largest leaf increased with increased amounts of fertilizer, with the best response was at 200 g. No significant differences were noted between the ratio of female flowers to total flowers in any treatments. In addition, the number of harvested fruits was highest with 160 g and 200 g of fertilizer. Taken together, the optimal blend ratio of rice husk charcoal to peat is 80:20, and the optimal amount of fertilizer with this ratio is 160 g.
文摘Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So, it is important to develop specific gene markers to allow rapid diagnosis by PCR. Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for Tomato yellow leaf curl virus (TYLCV) by the resistance gene Ty-1, in zucchini for Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus estirpe watermelon (PRSV-W), and in lettuce for Lettuce mosaic virus (LMV). Fragments of 249 bp were amplified only by resistant plants to TYLCV as the hybrids 2648 and Aguamiel, and not for varieties as Santa Cruz or Carina. It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle;for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant;for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Babá de Verão. Finally, it was observed that primers PK47F/R were able to check the Cabotiá seed hybrids of pumpkin Jabras.
基金Supported by Shandong Province Key R&D Program(2021LZGC015)Taishan Industry Leading Talent Project(LJNY201812)。
文摘[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong Province.The disease symptom was different from previous reports.This study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong Province.Primers were further designed for amplifying the full-length SLCCNV.The full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus disease.Since the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.
文摘The effects of fresh zucchini on nutritional status, and biological indicators for the prevention of cardiovascular disease in rats fed high-fat diets investigated in this study. Thirty Sprague Dawley rats were randomly divided into two main groups the first, negative control group Co (-) (n = 6), fed basal diet, The second group (n = 24) fed high-fat diet (containing basal diet + 5% tallow + 1% cholesterol + 0.02% bile salt). This group was divided into four subgroups each group 6 rats: group positive control co (+) fed high-fat diet only, group 1 (G (1)) fed high-fat diet plus 10% zucchini, group 2 (G (2) fed high-fat diet plus 15% zucchini and group 3 (G (3)) fed high-fat diet plus 20% zucchini. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured after eight wk. of experimental treatment. The pathologic changes of the heart, spleen and kidney were evaluated. SPSS, one way ANOVA was used to analyze the results. The results indicated that the mean values of body weight gain (BWG) and feed efficiency ratio (FIR) in G (2) and G (3) showed significant decrease compared to co (-), co (+) and G 1. The results showed that in groups (G1, G2 and G3) the relative weight of heart had significant increase when compared with control negative group. But liver and spleen weight had no significant difference when compared with control negative and positive group, the levels of TC, TG and LDL-C were significantly increased in co (+) (P < 0.05) Compared to co (-), all groups fed on high fat diet containing different levels of zucchini (10%, 15% and 20%) had significant decrease in TC and LDL-C compared with co (+), also G2 and G3 had improve significant in HDL-C when compared with co. (+). The groups fed on zucchini give results similar to group co. (-). The best treatment was zucchini (15% and 20%) which had lowest values of total lipid cholesterol and LDL-C, and the best values of HDL-C, HDL-C/TC % for all groups fed on high fat diet and zucchini increased non significantly (p ≤ 0.05) the HDL-C/TC % index compared to group co. (+). while, G2 and G3 gave significant decrease in LDL-C/HDL-C ratio compared to group co. (+). Morphologic changes of heart, spleen and kidney revealed that groups G2 and G3 had a similar preventive effect against CHD in this experimental model. In conclusion, results showed that zucchini had similar potential to attenuate CHD-related parameters in a mild oxidative stress induced by high-fat diet in rats.
文摘Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.
基金Supported by Key Research and Development Program of Shandong Province(2021LZGC015)Taishan Industry Leading Talents Program(LJNY201812)。
文摘This paper elaborates the symptom characteristics and control methods of virus diseases including squash leaf curl china virus(SLCCNV),watermelon mosaic virus(WMV),cucurbit chlorotic yellows virus(CCYV),cucumber mosaic virus(CMV)and zucchini yellow mosaic virus(ZYMV),as well as fungal diseases including zucchini powdery mildew,zucchini downy mildew and zucchini scab that harm zucchini production,in order to provide a theoretical basis and technical support for disease prevention and control of zucchini.
文摘The Zucchini yellow mosaic virus （ZYMV） （Potyvirus） on “zucchini” presents great economic importance for Malagasy farmers. Numerous aphid species （Hemiptera： Aphididae） spread viral particles, which are easily transmitted mechanically, too. Farmers ignored this disease, therefore, its control became extremely difficult with insecticides. This study aimed to evaluate efficacy of chili pepper extracts. Treatments vary as a function of its initiations and were repeated at weekly intervals until harvest. It was conducted in market gardens around the town of Antananarivo. The study compared four treatments and repeated three times： firstly, plots received a protection as soon as installation of culture （preventive protection）; secondly, plots received treatment with low infestation （about a quarter plants infested） （late protection）; thirdly, plots received treatment with high infestation （about half of plants infested） （latest protection）; lastly, plots without treatment （the control）. As a result, pepper extracts can provide a significant level to control against aphids attack, and permit to delay virus installation on plots, which received treatment as soon as implementation of culture. However, it cannot eradicate and cannot limit extend of virus disease on plots already infested. Economic analysis shows that preventive treatment of pepper extracts to fight virus attack provides an even greater return on its investment than all other terms. Thus, use of pepper extracts can reduce chemical treatment and pollution.
文摘The expansion of hydroponics in many countries of the world in the last few decades may be ascribed to the ability of soilless growing systems to avoid various problems arising from the use of the soil. Cucumber （Cucurnis sativus L.） and zucchini （Cucurbita pepo L.） plants were grown in closed-soilless culture under unheated-greenhouse conditions at the experimental farm of University of Tuscia, Central Italy to evaluate the effects of four substrates （rockwool, pumice, perlite, and cocofiber） on growth, yield and plant mineral composition. For both cultures, plants grown in cocofiber, perlite and pumice yielded more than those grown in rockwool. The better temperature regime in cocofiber, perlite and pumice was due to the greater thermal inertia compared to rockwool slabs. Thc concentration of N in zucchini and cucumber leaves was significantly higher in cocofiber, perlite and pumice in comparison to the rockwool treatment. The concentration of K was significantly affected by the substrate only for the zucchini squash with the highest value recorded on the organic substrate （cocofiber）, whereas the Ca concentration was significantly influenced by the growing media only for cucumber with the highest value observed on pumice. Finally, the lowest Mg concentration in leaf tissue was observed on plants grown with the rockwool substrate for both zucchini squash and cucumber. We can conclude that cocofiber, perlite and pumice are suitable for zucchini and cucumber production in closed soilless system, whereas the use of rockwool is more suitable for crops grown under heated greenhouse conditions.
文摘[Objectives]To select zucchini varieties suitable for cultivation in Zibo City and test its high-yield cultivation techniques.[Methods]Six zucchini varieties were introduced,and their commercial quality and yield were determined.[Results]The yield of Shengfeier,Xiuyu 170 and Xihulu 309 increased by 11.4%,6.9%and 4.6%,respectively compared with S68(control),and zucchini was straight,looked pleasing to the eye,and had strong disease resistance.[Conclusions]The zucchini varieties were selected and the high-quality,high-yield and high-efficiency cultivation techniques were integrated.
文摘以3种西葫芦(“碧玉”西葫芦BZ、“金皮香蕉”西葫芦JZ和“墨玉”西葫芦MZ)为研究对象,探究其营养成分、生物活性物质及体外抗氧化和降血糖活性差异。结果显示,3种西葫芦水分含量高(>95%),粗脂肪含量(<0.105 g/100 g FW)和总能量(<70.00 kJ/100 g FW)低,粗蛋白质含量适中(0.833~0.945 g/100 g FW),但含有较丰富的总碳水化合物和维生素C,其中BZ含量最高,达到3.08 g/100 g FW和12.04 mg AA/100 g FW;矿物质元素分析表明,3种西葫芦富含钾、磷和钙元素,且具有较高的钾钠比;氨基酸组成分析表明,3种西葫芦均含有多种氨基酸,且必需氨基酸占总氨基酸的34%以上;脂肪酸分析表明,BZ中含有更丰富的不饱和脂肪酸,占其脂肪酸总量的76.84%;BZ中总酚和总黄酮含量显著高于其他两个品种,并从BZ中共分离鉴定出11种酚类化合物,MZ西葫芦中共7种,JZ西葫芦中共5种,其中,BZ中的槲皮素-3,4'-二甲醚-7-O-芸香糖苷首次在西葫芦中鉴定发现。体外活性研究表明,BZ提取物具有更好的抗氧化和淀粉消化酶抑制活性。综上,西葫芦是一种营养丰富的低脂低能量蔬菜,但不同品种西葫芦的营养成分组成、含量和活性存在显著差异。
文摘为明确0.5%香菇多糖水剂对西葫芦病毒病的田间预防效果和治疗效果以及对作物的安全性。采用随机区组试验的方式在田间对西葫芦病毒病进行试验。结果表明,各个处理未见药害症状,对植株安全。0.5%香菇多糖水剂在6.0~10 g a.i./hm^(2)用量下可有效防治西葫芦病毒病,第1次药后21 d发病前药剂处理相对防效可达52.25%~74.02%,发病初期药剂处理相对防效可达54.69%~61.40%,且病害发生前使用相较于病害发生后使用能延缓病害蔓延。因此,0.5%香菇多糖水剂在田间可以有效防治西葫芦病毒病,建议推荐用量为10 g a.i./hm^(2),且在病毒病常发地区,建议发病前进行药剂预防,可显著提高防治效果。
基金Supported by Taishan Industry Leading Talent Program in Shandong Province(tscx202306156)Weifang Science and Technology Development Program(2024GX073).
文摘[Objectives]This study was conducted to detect and analyze tomato leaf curl New Delhi virus(ToLCNDV).[Methods]Through PCR detection,sequence analysis,and pathogenicity verification,tomato leaf curl New Delhi virus(ToLCNDV)was identified in zucchini exhibiting systemic disease symptoms during a 2024 outbreak in Qingzhou City,Shandong Province,and was designated as ToLCNDV-SD.[Results]Specific primer amplification showed that all eight diseased samples produced bands of 504 bp(DNA-A)and 892 bp(DNA-B).Sequencing analysis revealed that ToLCNDV-SD DNA-A shared 96.10%homology with an Indonesian melon isolate(LC421834.1),while DNA-B showed 88.31%homology with a Malaysian bitter gourd isolate(MW248678.1).Phylogenetic analysis indicated its closest relationship with Southeast Asian cucurbit-infecting isolates.Friction transmission tests confirmed that the virus could spread mechanically,inducing typical symptoms 14 d after inoculation with positive PCR detection.[Conclusions]This study provides important insights for understanding the epidemic mechanisms and control strategies of ToLCNDV in China.
