Maize(Zea mays L.)growth and yield are severely limited by drought stress worldwide.Stomata play crucial roles in transpiration and gas exchange and are thus essential for improving plant water-use efficiency(WUE)to h...Maize(Zea mays L.)growth and yield are severely limited by drought stress worldwide.Stomata play crucial roles in transpiration and gas exchange and are thus essential for improving plant water-use efficiency(WUE)to help plants deal with the threat of drought.In this study,we characterized the maize dsd1(decreased stomatal density 1)mutant,which showed defects in stomatal development,including guard mother cell differentiation,subsidiary cell formation and guard cell maturation.DSD1 encodes the basic helix-loop-helix transcription factor INDUCER OF CBF EXPRESSION b(ZmICEb)and is a homolog of ICE1 in Arabidopsis(Arabidopsis thaliana).DSD1/ZmICEb is expressed in stomatal file cells throughout stomatal development and plays a conserved role in stomatal development across maize and Arabidopsis.Mutations in DSD1/ZmICEb dramatically improved drought tolerance and WUE in maize and reduced yield losses under drought conditions.Therefore,DSD1/ZmICEb represents a promising candidate target gene for the genetic improvement of drought tolerance in maize by manipulating stomatal density.展开更多
基金supported by the Natural Science Foundation of China(Grant No.32160490,31860384,32360511,32460510,32170340)National Key Research&Development Program of China Grant(2022YFD1201801)+3 种基金Biological Breeding Project of Gansu Academy of Agricultural Sciences(2024GAAS24)Foundation of the Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations(lzujbky-2022-kb03)Foundation of Science and Technology of Gansu Province(21ZD10NF003-2,22ZD6NA049and24JRRA428)The Fundamental Research Funds for the Central Universities(lzujbky-2023-eyt02).
文摘Maize(Zea mays L.)growth and yield are severely limited by drought stress worldwide.Stomata play crucial roles in transpiration and gas exchange and are thus essential for improving plant water-use efficiency(WUE)to help plants deal with the threat of drought.In this study,we characterized the maize dsd1(decreased stomatal density 1)mutant,which showed defects in stomatal development,including guard mother cell differentiation,subsidiary cell formation and guard cell maturation.DSD1 encodes the basic helix-loop-helix transcription factor INDUCER OF CBF EXPRESSION b(ZmICEb)and is a homolog of ICE1 in Arabidopsis(Arabidopsis thaliana).DSD1/ZmICEb is expressed in stomatal file cells throughout stomatal development and plays a conserved role in stomatal development across maize and Arabidopsis.Mutations in DSD1/ZmICEb dramatically improved drought tolerance and WUE in maize and reduced yield losses under drought conditions.Therefore,DSD1/ZmICEb represents a promising candidate target gene for the genetic improvement of drought tolerance in maize by manipulating stomatal density.
