Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizi...Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizing network pharmacology,Gene Expression Omnibus(GEO)data,and molecular docking techniques.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),HERB,and the Encyclopedia of Traditional Chinese Medicine(ETCM)databases were used to screen out the active ingredients and related targets of Qingre Zaoshi Jiedu Recipe.The GeneCards database for disease targets at different stages of HR-HPV-related cervicitis–cancer were searched.The String platform to construct a protein-protein interaction network and identify key targets was utilized.Enrichment analysis of intersecting genes was performed using the DAVID database.The GSE149763 dataset from the GEO to identify differential genes involved in the transformation from cervicitis to cervical cancer by comparing cervicitis with cervical intraepithelial neoplasia III and cervical cancer was utilized.R language to generate volcano plots,heat maps,and key target expression trend charts were employed.Molecular docking of key pathway targets and main compounds of Qingre Zaoshi Jiedu Recipe for HR-HPV-related cervicitis–cancer was performed using AutoDock Vina.Results The study identified 185 main active ingredients of Qingre Zaoshi Jiedu Recipe.The protein–protein interaction network indicates that the core targets for interfering with HR-HPV-related inflammation–cancer transformation include TNF,IL-6,IL-1β,CXCL8,IL-1α,IFN-γ,IL-10,CCL2,CCL5,and CXCL10.KEGG pathway analysis indicates that Qingre Zaoshi Jiedu Recipe primarily affects HR-HPV-related inflammation–cancer transformation via the Toll-like receptor signaling pathway.GEO analysis identified the Toll-like receptor pathway as crucial across various stages of cervicitis–cancer lesions,with CXCL10 emerging as a key target.Molecular docking analysis revealed that the primary components of Qingre Zaoshi Jiedu Recipe effectively bind to TLR4.Conclusion Qingre Zaoshi Jiedu Recipe can interfere with HR-HPV-related cervicitis–cancer transformation by acting on TLR4 through the Toll-like receptor pathway.展开更多
[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discuss...[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.展开更多
基金supported by National Chinese Medicine Inheritance and Development Demonstration Pilot Project-Zhongshan Chinese Medicine Hospital Chinese Medicine Research Project(YN2024B005).
文摘Objective To investigate the active components and mechanisms by which Qingre Zaoshi Jiedu Recipe influences the transformation from cervical high-risk human papillomavirus(HR-HPV)-related cervicitis to cancer,utilizing network pharmacology,Gene Expression Omnibus(GEO)data,and molecular docking techniques.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),HERB,and the Encyclopedia of Traditional Chinese Medicine(ETCM)databases were used to screen out the active ingredients and related targets of Qingre Zaoshi Jiedu Recipe.The GeneCards database for disease targets at different stages of HR-HPV-related cervicitis–cancer were searched.The String platform to construct a protein-protein interaction network and identify key targets was utilized.Enrichment analysis of intersecting genes was performed using the DAVID database.The GSE149763 dataset from the GEO to identify differential genes involved in the transformation from cervicitis to cervical cancer by comparing cervicitis with cervical intraepithelial neoplasia III and cervical cancer was utilized.R language to generate volcano plots,heat maps,and key target expression trend charts were employed.Molecular docking of key pathway targets and main compounds of Qingre Zaoshi Jiedu Recipe for HR-HPV-related cervicitis–cancer was performed using AutoDock Vina.Results The study identified 185 main active ingredients of Qingre Zaoshi Jiedu Recipe.The protein–protein interaction network indicates that the core targets for interfering with HR-HPV-related inflammation–cancer transformation include TNF,IL-6,IL-1β,CXCL8,IL-1α,IFN-γ,IL-10,CCL2,CCL5,and CXCL10.KEGG pathway analysis indicates that Qingre Zaoshi Jiedu Recipe primarily affects HR-HPV-related inflammation–cancer transformation via the Toll-like receptor signaling pathway.GEO analysis identified the Toll-like receptor pathway as crucial across various stages of cervicitis–cancer lesions,with CXCL10 emerging as a key target.Molecular docking analysis revealed that the primary components of Qingre Zaoshi Jiedu Recipe effectively bind to TLR4.Conclusion Qingre Zaoshi Jiedu Recipe can interfere with HR-HPV-related cervicitis–cancer transformation by acting on TLR4 through the Toll-like receptor pathway.
基金Support by Major Science and Technology Projects of Guangdong Province (2004A20102002)~~
文摘[Objective]The aim was to study the effective factors on culturing Prunus salicina cv.Zaoshi embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three generations.On the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was discernible.On the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of culturing.On the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 generations.On the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture medium.The survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of Prunus salicina cv.Zaoshi,embryos rescue techniques and cross breading.
