A pair of new enantiomeric furoquinoline alkaloids,(±)-zanthonitidine A(1),together with nine known ones(2-10)were isolated from the radix of Zanthoxylum nitidum.Their chemical structures were elucidated based on...A pair of new enantiomeric furoquinoline alkaloids,(±)-zanthonitidine A(1),together with nine known ones(2-10)were isolated from the radix of Zanthoxylum nitidum.Their chemical structures were elucidated based on the extensive spectroscopic analysis.The racemic mixture of 1 was separated by chiral column chromatography,and the absolute configurations of (+)-1 and(-)-1 were determined by the comparison of experimental and calculated electronic circular dichroism spectra.Antibacterial activities of compounds 1-9 were evaluated,and compounds(+)-1,(-)-1,3,7 and 8 showed antibacterial activities against Bacillus subtilis,Enterococcus faecalis or Staphylococcus aureus.展开更多
[Objectives]This study was conducted to improve the germination rate of Zanthoxylum nitidum(Roxb.)seeds and shorten the germination period.[Methods]Different germination beds,seed soaking temperatures,seed soaking tim...[Objectives]This study was conducted to improve the germination rate of Zanthoxylum nitidum(Roxb.)seeds and shorten the germination period.[Methods]Different germination beds,seed soaking temperatures,seed soaking time and culture temperatures were set to study the effects on seed germination of Z.nitidum.[Results]The germination rate on the filter paper germination bed was stable and it was easy for the control of humidity.The germination rate of seeds soaked in warm water at 50℃for 24 h was about 53.33%.The germination rate of seeds cultured at 25℃was as high as 91.98%.[Conclusions]This study provides a theoretical basis for seedling breeding and large-scale production of Z.nitidum.展开更多
基金supported by the National Natural Science Foundation of China(31470428,21702231)the National New Drug Innovation Major Project of China(2017ZX09309027)the Program for Jiangsu Province Innovative Research Team,the Fund for Introduction of High-level Talents from China Pharmaceu-tical University and the Fundamental Research Funds for the Central Universities(2632017PY19).
文摘A pair of new enantiomeric furoquinoline alkaloids,(±)-zanthonitidine A(1),together with nine known ones(2-10)were isolated from the radix of Zanthoxylum nitidum.Their chemical structures were elucidated based on the extensive spectroscopic analysis.The racemic mixture of 1 was separated by chiral column chromatography,and the absolute configurations of (+)-1 and(-)-1 were determined by the comparison of experimental and calculated electronic circular dichroism spectra.Antibacterial activities of compounds 1-9 were evaluated,and compounds(+)-1,(-)-1,3,7 and 8 showed antibacterial activities against Bacillus subtilis,Enterococcus faecalis or Staphylococcus aureus.
基金Supported by General Project of Guangxi Science and Technology Department(2020GXNSFAA259043)Guangxi First-class Discipline of Traditional Chinese Medicine(Guangxi Education Scientific Research No.12,2018)+1 种基金Guangxi University of Chinese Medicine Project(05018028F2)Guangxi Zhuang Yao Medicine Key Laboratory(GXZYZZ2020A-04).
文摘[Objectives]This study was conducted to improve the germination rate of Zanthoxylum nitidum(Roxb.)seeds and shorten the germination period.[Methods]Different germination beds,seed soaking temperatures,seed soaking time and culture temperatures were set to study the effects on seed germination of Z.nitidum.[Results]The germination rate on the filter paper germination bed was stable and it was easy for the control of humidity.The germination rate of seeds soaked in warm water at 50℃for 24 h was about 53.33%.The germination rate of seeds cultured at 25℃was as high as 91.98%.[Conclusions]This study provides a theoretical basis for seedling breeding and large-scale production of Z.nitidum.
文摘为探究调控两面针Zanthoxylum nitidum种子休眠和萌发的分子机制,挖掘出关键的差异表达基因,该研究以未做处理(DS)、层积处理(SS)和发芽(GS)的两面针种胚为材料,利用Illumina Novaseq 6000平台进行高通量测序并进行系统的生物信息学分析。测序共获得61.41 Gb clean data,组装获得86386个unigene,平均长度773.49 bp。从3个比较组(SS vs DS、GS vs SS和GS vs DS)共筛选出29290个差异表达基因(DEGs),这些基因被注释在134条KEGG通路上。KEGG富集分析发现,植物激素信号转导通路是富集DEGs最多的一条通路,包含226个DEGs。从所有DEGs中鉴定出894个转录因子,分布在34个转录因子家族,这些转录因子也主要集中在植物激素信号转导和MAPK信号通路。进一步对12个DEGs进行RT-qPCR验证,结果表明转录组数据可靠。两面针种子休眠解除与萌发过程中大量与多糖分解、蛋白质合成、脂质代谢、激素信号转导的差异基因参与表达,这些基因涉及到多个代谢途径,构成复杂的休眠和萌发调控网络。该研究为解析两面针种子休眠和萌发分子机制奠定坚实基础。
