[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Meth...[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.展开更多
Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120...Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.展开更多
通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴...通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴性细菌都表现出显著的抗菌作用,乙酸乙酯提取物只对测试的部分细菌显示出抗菌作用,石油醚提取物不具有抗菌活性.甲醇提取物比乙酸乙酯提取物具有更强的抗菌作用和更广的抗菌谱.三种提取物对所有试验的真菌都没有显示出抗菌作用.展开更多
Objective:To study antifungal activity of a new ellagitannin isolated from the plant residues of Euphorbia antisyphilitica(E.antisyphilitica)Zucc in the wax extraction process.Methods:An extract was prepared from dehy...Objective:To study antifungal activity of a new ellagitannin isolated from the plant residues of Euphorbia antisyphilitica(E.antisyphilitica)Zucc in the wax extraction process.Methods:An extract was prepared from dehydrated and pulverized residues and fractionated by liquid chromatography on Amberilte XAD-16,until obtained an ellagitannin-rich ethanolic fraction which was treated by rotaevaporation to recover the ellagitannin as fine powder.An aqueous solution was prepared and treated through ionic exchange liquid chromatography(Q XL)and gel permeation chromatography(G 25).The ellagitannin-rich fraction was thermogravimetrically evaluated(TGA and DTA)to test the thermo-stability of ellagic acid(monomeric unit).Then ellagitannin powder was analyzed by infrared spectrospcopy to determinate the functional groups and.also mass spectroscopy was used to determine the molecular ion.Results:The principal functional groups of ellagitannin were determined,the molecular weight was 860.7 g/mol;and an effective antifungal activity against phytopathogenic fungi was demonstrated.Conclusions:It can be concluded that the new ellagitannin(860.7 g/mol)isolated from E.antisyphilitica Zucc is an effective antifungal agent against Alternaria alternata,Fusarium oxyzporum,Colletotrichum gloeosporoides and Rhizoctnia solani.展开更多
In this paper, the phenomena of the life cycle of Taxus cuspidata Sieb. et Zucc., such as germination, leafing, flowing seed mature,new shoot growth etc. were observed and measured. As a result, the phenological regul...In this paper, the phenomena of the life cycle of Taxus cuspidata Sieb. et Zucc., such as germination, leafing, flowing seed mature,new shoot growth etc. were observed and measured. As a result, the phenological regularity of Taxus cuspidata was found initially. At the same time,according to its five-day mean increment and height growth curve, the growing process of new shoot of Taxus cupidata was divided into three periods:slow period peak period and decline period. The paper supplied scientific data for the introduction of the rare and imininent variety, domestication,cultivating, expoiting, utilizing and protecting of Taxus cuspdata.展开更多
There are more than 250 kinds of Zanthoxylum plants in the world, of which about 40 species grow in China. In last 30 years, nearly 80 kinds of the plants have been studicd intensively and the results show that they a...There are more than 250 kinds of Zanthoxylum plants in the world, of which about 40 species grow in China. In last 30 years, nearly 80 kinds of the plants have been studicd intensively and the results show that they are of different chemical compositions and various physiological actions, such as having anthelmintic action, bacteriostasis,展开更多
[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were ...[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.展开更多
A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume rati...A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent （mixtures of acetone-water）. Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection（HPLC-DAD）. Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 rain on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines.展开更多
Lacebark pine （Pinus bungeana）, an evergreen conifer species native to China, is noted for its graceful profile. It has a height up to 30 m. Bark on young trees smooth, dull grey, scaling off in small patches like a...Lacebark pine （Pinus bungeana）, an evergreen conifer species native to China, is noted for its graceful profile. It has a height up to 30 m. Bark on young trees smooth, dull grey, scaling off in small patches like a plane tree, changing to chalky-white on old trunks, by which the species can be recognized from a long distance.展开更多
[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best ...[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.展开更多
[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify an...[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.展开更多
Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with chloro...Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with chloroform-acetone-formic acid-water (4∶4∶0.5∶0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284 nm and 384 nm, respectively. Results The linear regression equation of the calibration graph was y=7.02179x+4.5143, a linear regression correlative coefficient r=0.9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs.展开更多
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303093)the Key Project for Agricultural Improved Variety Breeding of Shandong Province (Lunongliang[2016]No.6~~
文摘[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.
基金Supported by Science and Technology Planning Project of Guang-dong Province (2007A020300002-6)~~
文摘Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.
文摘通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴性细菌都表现出显著的抗菌作用,乙酸乙酯提取物只对测试的部分细菌显示出抗菌作用,石油醚提取物不具有抗菌活性.甲醇提取物比乙酸乙酯提取物具有更强的抗菌作用和更广的抗菌谱.三种提取物对所有试验的真菌都没有显示出抗菌作用.
基金Supported by CONACYT's program of Food Science and Technology(Grant No.CONACYT-CONAFOR-2008-91633)
文摘Objective:To study antifungal activity of a new ellagitannin isolated from the plant residues of Euphorbia antisyphilitica(E.antisyphilitica)Zucc in the wax extraction process.Methods:An extract was prepared from dehydrated and pulverized residues and fractionated by liquid chromatography on Amberilte XAD-16,until obtained an ellagitannin-rich ethanolic fraction which was treated by rotaevaporation to recover the ellagitannin as fine powder.An aqueous solution was prepared and treated through ionic exchange liquid chromatography(Q XL)and gel permeation chromatography(G 25).The ellagitannin-rich fraction was thermogravimetrically evaluated(TGA and DTA)to test the thermo-stability of ellagic acid(monomeric unit).Then ellagitannin powder was analyzed by infrared spectrospcopy to determinate the functional groups and.also mass spectroscopy was used to determine the molecular ion.Results:The principal functional groups of ellagitannin were determined,the molecular weight was 860.7 g/mol;and an effective antifungal activity against phytopathogenic fungi was demonstrated.Conclusions:It can be concluded that the new ellagitannin(860.7 g/mol)isolated from E.antisyphilitica Zucc is an effective antifungal agent against Alternaria alternata,Fusarium oxyzporum,Colletotrichum gloeosporoides and Rhizoctnia solani.
文摘In this paper, the phenomena of the life cycle of Taxus cuspidata Sieb. et Zucc., such as germination, leafing, flowing seed mature,new shoot growth etc. were observed and measured. As a result, the phenological regularity of Taxus cuspidata was found initially. At the same time,according to its five-day mean increment and height growth curve, the growing process of new shoot of Taxus cupidata was divided into three periods:slow period peak period and decline period. The paper supplied scientific data for the introduction of the rare and imininent variety, domestication,cultivating, expoiting, utilizing and protecting of Taxus cuspdata.
文摘There are more than 250 kinds of Zanthoxylum plants in the world, of which about 40 species grow in China. In last 30 years, nearly 80 kinds of the plants have been studicd intensively and the results show that they are of different chemical compositions and various physiological actions, such as having anthelmintic action, bacteriostasis,
基金Supported by Guangxi Colleges and Universities Scientific Research Funding Project(NO.YB2014192)Guangxi Zhuang Medicine Quality Evaluation and Standard Research Project(NO.MZY2013023)+2 种基金Guangxi Scientific Research and Technological Development Program(GKG14124002-11-1)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutes-Zhuang Medicine Basic and Clinical Innovation Team(GJR [2014]07)College Students'Innovation and Enterpreneurship Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35)
文摘[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.
基金Project (04JJ3081) supported by Hunan Province Natural Science Foundation of China
文摘A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent （mixtures of acetone-water）. Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection（HPLC-DAD）. Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 rain on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines.
文摘Lacebark pine （Pinus bungeana）, an evergreen conifer species native to China, is noted for its graceful profile. It has a height up to 30 m. Bark on young trees smooth, dull grey, scaling off in small patches like a plane tree, changing to chalky-white on old trunks, by which the species can be recognized from a long distance.
基金Supported by 2014 Sichuan Science and Technology Support Plan Program(2014SZ0131)Education Innovation Project of Southwest Minzu University(2015)
文摘[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.
基金Supported by Study on Quality Evaluation and Standards of Guangxi Yao Medicine(MZY2012015)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+2 种基金Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutions:Zhuang Medicine Foundation and Clinical Innovation Team(GJR[2014]07)Student Research Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35).Wei WEI(1981-),male,P.R.China,experimentalist,devoted to research about Tradition Chinese medicine and ethnic drug
文摘[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.
文摘Objective A method of TLC-fluorescence spectrophotometry was established to assay the content of polydatin in polygonum cuspidatum sieb. et zucc. Methods: Polydatin was extracted by methanol and separated with chloroform-acetone-formic acid-water (4∶4∶0.5∶0.2) by thin layer chromatography. The excitation wavelength and emission wavelength were 284 nm and 384 nm, respectively. Results The linear regression equation of the calibration graph was y=7.02179x+4.5143, a linear regression correlative coefficient r=0.9936. Conclusion This method was proved simple, stable and sensitive. It can be used in quality control of herbs.
