目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,...目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,并分为溃疡对照(CU)组、糖尿病溃疡(DU)组、四妙勇安汤组(DU-四妙组)、愈疡灵组(DU-愈疡灵组)、四妙勇安汤+愈疡灵组(DU-四妙+愈疡灵组),每组各15只。观察造模后第3、7、14 d各组创面愈合情况和织形态结构的变化的变化,采用ELISA法、RT-PCR法检测创面组织β-catenin、糖原合成激酶-3β(GSK-3β)蛋白及m RNA的表达水平。结果:DU组大鼠与CU组大鼠相比,第3、7、14 d的创面愈合率明显降低[(5.21±0.94)%vs(20.87±1.39)%,(31.70±1.21)%vs (49.99±1.58)%,(62.20±1.37)%vs (91.60±1.58)%, P <0.05];创面组织中β-catenin蛋白表达[(5.14±0.56) vs (9.04±0.65),(5.83±0.43) vs (10.08±0.35),(6.55±0.58) vs (12.77±0.30), P <0.05]及mRNA表达[(4.40±0.35) vs (6.92±0.16),(5.20±0.49) vs (7.12±0.48),(6.06±0.47) vs (9.24±0.55), P <0.05]均较低,而GSK-3β蛋白表达[(15.52±1.00) vs (7.04±0.53),(14.58±0.45) vs (6.05±0.23),(13.37±0.62) vs (4.49±0.37), P <0.05]及mRNA表达[(7.07±0.29) vs (3.55±0.32),(6.64±0.47) vs (3.00±0.28),(5.68±0.46) vs (1.98±0.19), P <0.05]则较高。与DU组大鼠相比,各治疗组大鼠各时间点的创面愈合率、创面组织中β-catenin蛋白及mRNA的表达水平均升高,而GSK-3β蛋白及mRNA的表达水平则降低,且治疗组中,以DU-四妙+愈疡灵组改善最明显。结论:具有凉血散瘀作用的四妙勇安汤及愈疡灵软膏均可通过干预Wnt/β-catenint通路的表达促进DU大鼠创面愈合,两者同用的效果优于单用其中一种。展开更多
High effect flocculant compound bacteria were screened out with the product bacteria of flocculant in soil. Their system appraising study shows that two were actionomyces and the other was microzyme among the three ba...High effect flocculant compound bacteria were screened out with the product bacteria of flocculant in soil. Their system appraising study shows that two were actionomyces and the other was microzyme among the three bacteria. According to nSrDNA, the three bacteria were marked at a molecule level and the system growth tree was established, ensuring the position of compound bacteria at the molecule level. The purificant which was made of the compound bacteria has spreading value because of its excellent clearing effect for organic and inorganic polluted water.展开更多
文摘目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,并分为溃疡对照(CU)组、糖尿病溃疡(DU)组、四妙勇安汤组(DU-四妙组)、愈疡灵组(DU-愈疡灵组)、四妙勇安汤+愈疡灵组(DU-四妙+愈疡灵组),每组各15只。观察造模后第3、7、14 d各组创面愈合情况和织形态结构的变化的变化,采用ELISA法、RT-PCR法检测创面组织β-catenin、糖原合成激酶-3β(GSK-3β)蛋白及m RNA的表达水平。结果:DU组大鼠与CU组大鼠相比,第3、7、14 d的创面愈合率明显降低[(5.21±0.94)%vs(20.87±1.39)%,(31.70±1.21)%vs (49.99±1.58)%,(62.20±1.37)%vs (91.60±1.58)%, P <0.05];创面组织中β-catenin蛋白表达[(5.14±0.56) vs (9.04±0.65),(5.83±0.43) vs (10.08±0.35),(6.55±0.58) vs (12.77±0.30), P <0.05]及mRNA表达[(4.40±0.35) vs (6.92±0.16),(5.20±0.49) vs (7.12±0.48),(6.06±0.47) vs (9.24±0.55), P <0.05]均较低,而GSK-3β蛋白表达[(15.52±1.00) vs (7.04±0.53),(14.58±0.45) vs (6.05±0.23),(13.37±0.62) vs (4.49±0.37), P <0.05]及mRNA表达[(7.07±0.29) vs (3.55±0.32),(6.64±0.47) vs (3.00±0.28),(5.68±0.46) vs (1.98±0.19), P <0.05]则较高。与DU组大鼠相比,各治疗组大鼠各时间点的创面愈合率、创面组织中β-catenin蛋白及mRNA的表达水平均升高,而GSK-3β蛋白及mRNA的表达水平则降低,且治疗组中,以DU-四妙+愈疡灵组改善最明显。结论:具有凉血散瘀作用的四妙勇安汤及愈疡灵软膏均可通过干预Wnt/β-catenint通路的表达促进DU大鼠创面愈合,两者同用的效果优于单用其中一种。
基金the "973" Project of China ( No 2004CB418505) Innovation Foundation of Jilin University ( No419070200045)
文摘High effect flocculant compound bacteria were screened out with the product bacteria of flocculant in soil. Their system appraising study shows that two were actionomyces and the other was microzyme among the three bacteria. According to nSrDNA, the three bacteria were marked at a molecule level and the system growth tree was established, ensuring the position of compound bacteria at the molecule level. The purificant which was made of the compound bacteria has spreading value because of its excellent clearing effect for organic and inorganic polluted water.
