目的:结合特征图谱、多指标含量测定、化学计量学技术以及一测多评法(quantitative analysis of multi-components by single-marker,QAMS)实现药材、中间体和制剂的多成分同步测定,用于评价益脉康制剂的整体质量。方法:采用Waters XBri...目的:结合特征图谱、多指标含量测定、化学计量学技术以及一测多评法(quantitative analysis of multi-components by single-marker,QAMS)实现药材、中间体和制剂的多成分同步测定,用于评价益脉康制剂的整体质量。方法:采用Waters XBridge BEH C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈和0.2%磷酸水溶液,梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,检测波长327 nm。建立HPLC特征图谱,进行相似度分析。采用一测多评法定量分析19批样品中5种成分的含量,研究质量传递过程,并采用雷达图分析、聚类分析、主成分分析和正交偏最小二乘-判别法对含量数据进行分析。结果:建立了19批益脉康制剂HPLC特征图谱,确认了5种成分,分别是新绿原酸、绿原酸、隐绿原酸、野黄芩苷和灯盏花甲素,其含量在线性范围内呈良好的线性关系(r≥0.9990),精密度、重复性、稳定性、加样回收率试验均良好。药材-中间体-制剂各成分的一测多评计算结果与外标法无显著差异;化学计量学分析确定了2个差异标志物,分别是野黄芩苷和灯盏花甲素。结论:所建分析方法稳定、可行,特征图谱、HPLC-QAMS多指标成分含量测定结合化学计量学的方法可实现益脉康制剂的整体质量评价,为其质量控制提供参考。展开更多
Objective To explore the mechanism by which QingguangannIFormula(QGAI)inhibits the apoptosis of retinal ganglion cells(RCCs)in glaucoma through the regulation of B-cell lymphoma 2(Bcl-2)/Bcl-2-associated X protein(Bax...Objective To explore the mechanism by which QingguangannIFormula(QGAI)inhibits the apoptosis of retinal ganglion cells(RCCs)in glaucoma through the regulation of B-cell lymphoma 2(Bcl-2)/Bcl-2-associated X protein(Bax)/Cysteine-aspartic protease 3(Caspase-3)by Brain-specific homeobox/POU domain protein 3B(Brn-3b).Methods After one week of acclimatization sixty healthy male SPF SD rats were randomly divided into control group,model group,lowdose,medium-dose,and high-dose QGA II group,and Yimaikang group,10 in each group.Except for the control group,elevated intraocular pressure was induced in the other groups by cauterizing the episcleral veins.The control and model groups were treated with saline,while the Yimaikang(9.67 g·kg^(-1).d^(-1))and QGA II(3.375,6.75,13.5 g·kg^(-1).d^(-1))groups received their respective treatments via gavage for 4 weeks.Intraocular pressure was measured preoperatively and at 30 minutes,1,7,14,and 28 days postoperatively.After euthanizing the rats,retinal tissues were isolated for histological examination and analysis of RGC apoptosis and the expression of Brn-3b,Caspase-3,Bcl-2,and Bax proteins and mRNA.Results Intraocular pressure was significantly elevated in all model groups compared to baseline(P<0.05)and remained high at 4 weeks.Compared to the model group,the QGA II and Yimaikang groups exhibited significantly reduced RGC apoptosis(P<0.01).Among them,the high-dose QGA II group showed the lowest RGC apoptosis rates(P<0.05).Compared to the model group,expressions of Caspase-3 and Bax proteins and mRNA increased in the Yimaikang and high-dose QGA II groups(P<0.01),while expressions of Brn-3b and Bcl-2 proteins and mRNA significantly decreased(P<0.01).Conclusion QGA I inhibits RGC apoptosis by regulating Brn-3b,Bax/Bcl-2/Caspase-3,withefficacyycomparableto Yimaikang.展开更多
文摘目的:结合特征图谱、多指标含量测定、化学计量学技术以及一测多评法(quantitative analysis of multi-components by single-marker,QAMS)实现药材、中间体和制剂的多成分同步测定,用于评价益脉康制剂的整体质量。方法:采用Waters XBridge BEH C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈和0.2%磷酸水溶液,梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,检测波长327 nm。建立HPLC特征图谱,进行相似度分析。采用一测多评法定量分析19批样品中5种成分的含量,研究质量传递过程,并采用雷达图分析、聚类分析、主成分分析和正交偏最小二乘-判别法对含量数据进行分析。结果:建立了19批益脉康制剂HPLC特征图谱,确认了5种成分,分别是新绿原酸、绿原酸、隐绿原酸、野黄芩苷和灯盏花甲素,其含量在线性范围内呈良好的线性关系(r≥0.9990),精密度、重复性、稳定性、加样回收率试验均良好。药材-中间体-制剂各成分的一测多评计算结果与外标法无显著差异;化学计量学分析确定了2个差异标志物,分别是野黄芩苷和灯盏花甲素。结论:所建分析方法稳定、可行,特征图谱、HPLC-QAMS多指标成分含量测定结合化学计量学的方法可实现益脉康制剂的整体质量评价,为其质量控制提供参考。
文摘Objective To explore the mechanism by which QingguangannIFormula(QGAI)inhibits the apoptosis of retinal ganglion cells(RCCs)in glaucoma through the regulation of B-cell lymphoma 2(Bcl-2)/Bcl-2-associated X protein(Bax)/Cysteine-aspartic protease 3(Caspase-3)by Brain-specific homeobox/POU domain protein 3B(Brn-3b).Methods After one week of acclimatization sixty healthy male SPF SD rats were randomly divided into control group,model group,lowdose,medium-dose,and high-dose QGA II group,and Yimaikang group,10 in each group.Except for the control group,elevated intraocular pressure was induced in the other groups by cauterizing the episcleral veins.The control and model groups were treated with saline,while the Yimaikang(9.67 g·kg^(-1).d^(-1))and QGA II(3.375,6.75,13.5 g·kg^(-1).d^(-1))groups received their respective treatments via gavage for 4 weeks.Intraocular pressure was measured preoperatively and at 30 minutes,1,7,14,and 28 days postoperatively.After euthanizing the rats,retinal tissues were isolated for histological examination and analysis of RGC apoptosis and the expression of Brn-3b,Caspase-3,Bcl-2,and Bax proteins and mRNA.Results Intraocular pressure was significantly elevated in all model groups compared to baseline(P<0.05)and remained high at 4 weeks.Compared to the model group,the QGA II and Yimaikang groups exhibited significantly reduced RGC apoptosis(P<0.01).Among them,the high-dose QGA II group showed the lowest RGC apoptosis rates(P<0.05).Compared to the model group,expressions of Caspase-3 and Bax proteins and mRNA increased in the Yimaikang and high-dose QGA II groups(P<0.01),while expressions of Brn-3b and Bcl-2 proteins and mRNA significantly decreased(P<0.01).Conclusion QGA I inhibits RGC apoptosis by regulating Brn-3b,Bax/Bcl-2/Caspase-3,withefficacyycomparableto Yimaikang.
