Objective: To investigate the effect of recombinant staphylokinase (r-Sak) and the Chinese medicine Yihuo Qingyi Decoction (益活凊胰汤 Herbal decoction for severe acute pancreatitis) in the treatment of the severe acu...Objective: To investigate the effect of recombinant staphylokinase (r-Sak) and the Chinese medicine Yihuo Qingyi Decoction (益活凊胰汤 Herbal decoction for severe acute pancreatitis) in the treatment of the severe acute pancreatitis (SAP) in rats, and to observe the synergistic effect of the two. Methods: One hundred and sixty-two adult male SD rats with the body mass of 250–280 g were randomly divided into the following 5 groups: sham operation group (n=18), control group (n=36), Yihuo Qingyi Decoction treatment group (n=36), r-Sak treatment group (n=36), and Yihuo Qingyi Decoction plus r-Sak treatment group (n=36). The SAP ratmodel was prepared by retrograde injection of 5% sodium taurocholate into the cholangiopancreatic duct. Two days before modeling, Yihuo Qingyi Decoction was intragastrically administrated, and r-Sak was intraperitoneally injected. The survival rate within 18 h after modeling was determined. The pancreatic blood flow, the weight of ascites, and the serum amylase and lipase were investigated at 6 h, 12 h, and 18kh after modeling, and the pancreatic tissue was examined under light microscopy to see its pathological change. Results: The 18 h survival count of group A,B,C,D and E rats was 9,2,6,7 and 8 respectively. After r-Sak and Yihuo Qingyi Decoction intervention, the serum amylase and lipase and the weight of ascites were significantly decreased, especially in group E.18 h after modeling, the level of the serum amylase and lipase and the weight of ascites in group E was 1 100±118 U·L-1,1 000±150 U·L-1 and 13.40±1.80 g respectively, obviously lower than that of group B (P<0.05).After SAP was induced, the pancreatic blood flow showed a tendency to decrease, but the decrease extent in the treatment groups was smaller than that in the control group.18h after modeling, the pancreatic blood flow in group B and group E was 30.16±8.96 mL·100 g-1·min-1,and 129.10±42.58 mL·100 g-1·min-1 respectively, there was significant difference (P<0.05). The pathological change of the pancreatic tissue was alleviated in the treatment groups. Conclusion: Both r-Sak and Yihuo Qingyi Decoction play a beneficial role in the treatment of rat SAP and there is a synergistic effect between the two.展开更多
Objective To investigate the efficacy of Qingfei Yihuo Capsules(清肺抑火胶囊,QYCs)in preventing the air pollution associated exacerbation of chronic obstructive pulmonary disease(COPD).Methods This was a prospective,p...Objective To investigate the efficacy of Qingfei Yihuo Capsules(清肺抑火胶囊,QYCs)in preventing the air pollution associated exacerbation of chronic obstructive pulmonary disease(COPD).Methods This was a prospective,parallel,single-blind,randomized,placebo-controlled trial.Sixty patients with stable Group D COPD were randomly allocated to receive either oral QYCs(intervention group)or placebos(control group,30 cases per group)for 15 days in the presumed high-incidence air pollution season and followed-up for 1 year.Both groups were given individualized Western medicine therapy according to the Global Initiative for Chronic Obstructive Lung Disease criteria as usual.Total and separate numbers of acute exacerbation(AE)associated with striking air pollution was the primary outcomes.Secondary outcomes included total numbers of deteriorating respiratory symptoms and separate numbers associated with striking air pollution,as well as scores of COPD Assessment Test(CAT)and modified Medical Research Council Scale(mMRC).Results All the 60 patients completed the study.There was no statistical significance in total numbers of AE between the two groups(P>0.05).Compared with the control group,a significant reduction in air-pollution associated numbers of deteriorated respiratory symptoms was observed in the intervention group(1.9–1.2 vs.3.6–2.4,P<0.01).At the end of follow-up,there was no significant difference in CAT and mMRC scores between the two groups(P>0.05).Only 2 patients in the intervention group reported diarrhea and recovered after drug discontinuance.Conclusion For patients with Group D COPD,oral QYCs in high-incidence season of air pollution can effectively mitigate respiratory symptoms associated with air pollution,although there was no evidence that it had a significant reductive effect on AEs.(Registered at Chinese Clinical Trial Registry,registration No.ChiCTR-IOR-17013827).展开更多
基金supported by grants from "Tenth five-years" 863 Biological High-tech Foundation (No.2001AA215161)
文摘Objective: To investigate the effect of recombinant staphylokinase (r-Sak) and the Chinese medicine Yihuo Qingyi Decoction (益活凊胰汤 Herbal decoction for severe acute pancreatitis) in the treatment of the severe acute pancreatitis (SAP) in rats, and to observe the synergistic effect of the two. Methods: One hundred and sixty-two adult male SD rats with the body mass of 250–280 g were randomly divided into the following 5 groups: sham operation group (n=18), control group (n=36), Yihuo Qingyi Decoction treatment group (n=36), r-Sak treatment group (n=36), and Yihuo Qingyi Decoction plus r-Sak treatment group (n=36). The SAP ratmodel was prepared by retrograde injection of 5% sodium taurocholate into the cholangiopancreatic duct. Two days before modeling, Yihuo Qingyi Decoction was intragastrically administrated, and r-Sak was intraperitoneally injected. The survival rate within 18 h after modeling was determined. The pancreatic blood flow, the weight of ascites, and the serum amylase and lipase were investigated at 6 h, 12 h, and 18kh after modeling, and the pancreatic tissue was examined under light microscopy to see its pathological change. Results: The 18 h survival count of group A,B,C,D and E rats was 9,2,6,7 and 8 respectively. After r-Sak and Yihuo Qingyi Decoction intervention, the serum amylase and lipase and the weight of ascites were significantly decreased, especially in group E.18 h after modeling, the level of the serum amylase and lipase and the weight of ascites in group E was 1 100±118 U·L-1,1 000±150 U·L-1 and 13.40±1.80 g respectively, obviously lower than that of group B (P<0.05).After SAP was induced, the pancreatic blood flow showed a tendency to decrease, but the decrease extent in the treatment groups was smaller than that in the control group.18h after modeling, the pancreatic blood flow in group B and group E was 30.16±8.96 mL·100 g-1·min-1,and 129.10±42.58 mL·100 g-1·min-1 respectively, there was significant difference (P<0.05). The pathological change of the pancreatic tissue was alleviated in the treatment groups. Conclusion: Both r-Sak and Yihuo Qingyi Decoction play a beneficial role in the treatment of rat SAP and there is a synergistic effect between the two.
基金Supported by the National Key Research and Development Program of China(No.2018YFC1313600)Medical Guide Project of Shan ghai Science and Tech no logy Commissi on(No.14401970700)+1 种基金Traditional Chinese Medicine Research Grant from Committee on Health and Family Planning in Shanghai(No.2014LP004A)and the Industry-University-Research-Medicine Modern Chinese Medicine Project of Shanghai Science and Technology Commission(No.15DZ1900202)。
文摘Objective To investigate the efficacy of Qingfei Yihuo Capsules(清肺抑火胶囊,QYCs)in preventing the air pollution associated exacerbation of chronic obstructive pulmonary disease(COPD).Methods This was a prospective,parallel,single-blind,randomized,placebo-controlled trial.Sixty patients with stable Group D COPD were randomly allocated to receive either oral QYCs(intervention group)or placebos(control group,30 cases per group)for 15 days in the presumed high-incidence air pollution season and followed-up for 1 year.Both groups were given individualized Western medicine therapy according to the Global Initiative for Chronic Obstructive Lung Disease criteria as usual.Total and separate numbers of acute exacerbation(AE)associated with striking air pollution was the primary outcomes.Secondary outcomes included total numbers of deteriorating respiratory symptoms and separate numbers associated with striking air pollution,as well as scores of COPD Assessment Test(CAT)and modified Medical Research Council Scale(mMRC).Results All the 60 patients completed the study.There was no statistical significance in total numbers of AE between the two groups(P>0.05).Compared with the control group,a significant reduction in air-pollution associated numbers of deteriorated respiratory symptoms was observed in the intervention group(1.9–1.2 vs.3.6–2.4,P<0.01).At the end of follow-up,there was no significant difference in CAT and mMRC scores between the two groups(P>0.05).Only 2 patients in the intervention group reported diarrhea and recovered after drug discontinuance.Conclusion For patients with Group D COPD,oral QYCs in high-incidence season of air pollution can effectively mitigate respiratory symptoms associated with air pollution,although there was no evidence that it had a significant reductive effect on AEs.(Registered at Chinese Clinical Trial Registry,registration No.ChiCTR-IOR-17013827).
