AIM: To determine changes in the antibiotic resistance of Helicobacter pylori(H. pylori) in southern Taiwan after failure of first-line standard triple therapy.METHODS: We analyzed 137 H. pylori-infected isolates from...AIM: To determine changes in the antibiotic resistance of Helicobacter pylori(H. pylori) in southern Taiwan after failure of first-line standard triple therapy.METHODS: We analyzed 137 H. pylori-infected isolates from patients who experienced eradication failure after standard first-line triple therapy from January2010 to December 2014. The H. pylori strains were tested for susceptibility to amoxicillin, clarithromycin,levofloxacin, metronidazole and tetracycline using the E-test method. The minimal inhibitory concentration(MIC) was determined by the agar dilution test.MIC values of ≥ 0.5, ≥ 1, ≥ 1, ≥ 4 and ≥ 8 mg/L were considered to be the resistance breakpoints for amoxicillin, clarithromycin, levofloxacin, tetracycline and metronidazole, respectively.RESULTS: A high resistance rate was found for clarithromycin(65%-75%) and metronidazole(30%-40%)among patients who failed first-line standard therapy.The resistance levels to amoxicillin and tetracycline remained very low; however, levofloxacin resistance was as high as 37.5% in 2010 but did not increase any further during the past 5 years. The rates of resistance to these antibiotics did not show a statistically significant upward or downward trend.CONCLUSION: Antibiotic resistance of H. pylori remains a problem for the effective eradication of this pathogen and its associated diseases in Taiwan. High clarithromycin resistance indicated that this antibiotic should not be prescribed as a second-line H. pylori eradication therapy. Moreover, levofloxacin-based second-line therapy should be used cautiously, and the local resistance rates should be carefully monitored.展开更多
2008 may be the most startling year in the past decade for chemical players.Sharp fluctuations showed by the crude oil price would undoubtedly have sur-passed the predictions for many analysts,chemical prices showed a...2008 may be the most startling year in the past decade for chemical players.Sharp fluctuations showed by the crude oil price would undoubtedly have sur-passed the predictions for many analysts,chemical prices showed a more aston-ished free falling.Managers and traders have doubted that the ceiling prices everreached in the summer would have been a dream instead of a reality.Both theabrupt price failing and 50% or lower operation rate have indicated 2009 will展开更多
Yeast YIp\|type expression recombinant plasmid(pCMA2\|1) was constructed. The expression of α\|acetolactate decarboxylase gene from \%Bacillus subtilis\% was controled by \%CUP1\% promoter and its own terminator. The...Yeast YIp\|type expression recombinant plasmid(pCMA2\|1) was constructed. The expression of α\|acetolactate decarboxylase gene from \%Bacillus subtilis\% was controled by \%CUP1\% promoter and its own terminator. The recombinant plasmid pCMA2\|1 was introduced into the brewer’s yeast PJ3\|5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3\|5 didn’t produce α\|acetolactate decarboxylase(ALDC), where as the activity of α\|ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by α\|ALDC in transformant.展开更多
文摘AIM: To determine changes in the antibiotic resistance of Helicobacter pylori(H. pylori) in southern Taiwan after failure of first-line standard triple therapy.METHODS: We analyzed 137 H. pylori-infected isolates from patients who experienced eradication failure after standard first-line triple therapy from January2010 to December 2014. The H. pylori strains were tested for susceptibility to amoxicillin, clarithromycin,levofloxacin, metronidazole and tetracycline using the E-test method. The minimal inhibitory concentration(MIC) was determined by the agar dilution test.MIC values of ≥ 0.5, ≥ 1, ≥ 1, ≥ 4 and ≥ 8 mg/L were considered to be the resistance breakpoints for amoxicillin, clarithromycin, levofloxacin, tetracycline and metronidazole, respectively.RESULTS: A high resistance rate was found for clarithromycin(65%-75%) and metronidazole(30%-40%)among patients who failed first-line standard therapy.The resistance levels to amoxicillin and tetracycline remained very low; however, levofloxacin resistance was as high as 37.5% in 2010 but did not increase any further during the past 5 years. The rates of resistance to these antibiotics did not show a statistically significant upward or downward trend.CONCLUSION: Antibiotic resistance of H. pylori remains a problem for the effective eradication of this pathogen and its associated diseases in Taiwan. High clarithromycin resistance indicated that this antibiotic should not be prescribed as a second-line H. pylori eradication therapy. Moreover, levofloxacin-based second-line therapy should be used cautiously, and the local resistance rates should be carefully monitored.
文摘2008 may be the most startling year in the past decade for chemical players.Sharp fluctuations showed by the crude oil price would undoubtedly have sur-passed the predictions for many analysts,chemical prices showed a more aston-ished free falling.Managers and traders have doubted that the ceiling prices everreached in the summer would have been a dream instead of a reality.Both theabrupt price failing and 50% or lower operation rate have indicated 2009 will
文摘Yeast YIp\|type expression recombinant plasmid(pCMA2\|1) was constructed. The expression of α\|acetolactate decarboxylase gene from \%Bacillus subtilis\% was controled by \%CUP1\% promoter and its own terminator. The recombinant plasmid pCMA2\|1 was introduced into the brewer’s yeast PJ3\|5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3\|5 didn’t produce α\|acetolactate decarboxylase(ALDC), where as the activity of α\|ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by α\|ALDC in transformant.
文摘目的建立测定富硒酵母中的硒代蛋氨酸含量的高效液相色谱-电化学检测器检测法。方法色谱柱采用Agilent Eclipse plus C18柱(150 mm×3.0 mm,3.5μm),流动相为100 mmol/L柠檬酸缓冲盐(p H=2.75,含300 mg/L辛烷基磺酸钠)-甲醇(85∶15),检测电压为500 m V(ECD1)和700 m V(ECD2)。结果硒代蛋氨酸质量浓度在1~300μmol/L范围内与峰面积线性关系良好(r^2=0.999 6),检出限为0.30μmol/L,加样回收率为101.30%,RSD为2.30%(n=6)。结论该方法操作简便,灵敏度高,重复性好,可用于富硒酵母中硒代蛋氨酸的检测。
