Mature wild-type yellow catfish( Pelteobagrus fulvidraco Richardson) individuals with excellent traits have been screened from the Yangtze River as broodstock to establish the germplasm bank of pure-line yellow catfis...Mature wild-type yellow catfish( Pelteobagrus fulvidraco Richardson) individuals with excellent traits have been screened from the Yangtze River as broodstock to establish the germplasm bank of pure-line yellow catfish by artificial gynogenesis technique and hormonal sex reversal method. Based on pure lines of yellow catfish,super-males and physiological females of yellow catfish were selected by GMT technique,hormonal sex reversal method and test-crossing to establish the germplasm bank of YY physiological females( YY♀). The propagation system of male yellow catfish( XY♂) was established based on the combination of super-males( YY♂) and pure-line female( XX♀) for large-scale production of pure-line yellow catfish males,which effectively overcome the disadvantages in the production of common fingerlings and male fingerlings using low-grade fish with slow growth and small size at the bottom of the fish grader as broodfish,such as progeny germplasm degeneration,loss of growth vigor and reduction of breeding benefits. The technical route of breeding pure-line YY physiological females( YY♀) and YY super-males( YY♂) laid the foundation for large-scale production of environmentally friendly yellow catfish males with pure germplasm and strong stress resistance,and provided an efficient,stable,healthy,environmentally friendly,energy-saving and income-increasing approach for sustainable development of yellow catfish breeding industry.展开更多
为了提高中度嗜酸放线菌YY21产纤溶酶的产量,本文通过单因素实验对其液体发酵培养基和培养条件进行了研究。结果表明:发酵培养基组成为小米粉1.7%,葡萄糖2%,碳酸钙0.2%,氯化钠0.5%,蛋白胨0.6%;发酵条件为250 m L三角瓶装培养基50 m L,...为了提高中度嗜酸放线菌YY21产纤溶酶的产量,本文通过单因素实验对其液体发酵培养基和培养条件进行了研究。结果表明:发酵培养基组成为小米粉1.7%,葡萄糖2%,碳酸钙0.2%,氯化钠0.5%,蛋白胨0.6%;发酵条件为250 m L三角瓶装培养基50 m L,接种量5%,在22℃、转速160 r/min的条件下振荡培养4 d。在最适条件下,放线菌YY21液体发酵产物在血纤维蛋白平板上形成溶圈面积可达到164.38 mm^2,较初始产酶活力(溶圈面积87.58 mm^2)提高了88%,发酵时间缩短了3 d。通过对液体发酵培养基和培养条件的研究有效地提高了中度嗜酸放线菌YY21产纤溶酶的产量。展开更多
基金Supported by Implementation Plan of Subsidy Project for the Reform and Construction of Grassroots Agricultural Technology Extension System
文摘Mature wild-type yellow catfish( Pelteobagrus fulvidraco Richardson) individuals with excellent traits have been screened from the Yangtze River as broodstock to establish the germplasm bank of pure-line yellow catfish by artificial gynogenesis technique and hormonal sex reversal method. Based on pure lines of yellow catfish,super-males and physiological females of yellow catfish were selected by GMT technique,hormonal sex reversal method and test-crossing to establish the germplasm bank of YY physiological females( YY♀). The propagation system of male yellow catfish( XY♂) was established based on the combination of super-males( YY♂) and pure-line female( XX♀) for large-scale production of pure-line yellow catfish males,which effectively overcome the disadvantages in the production of common fingerlings and male fingerlings using low-grade fish with slow growth and small size at the bottom of the fish grader as broodfish,such as progeny germplasm degeneration,loss of growth vigor and reduction of breeding benefits. The technical route of breeding pure-line YY physiological females( YY♀) and YY super-males( YY♂) laid the foundation for large-scale production of environmentally friendly yellow catfish males with pure germplasm and strong stress resistance,and provided an efficient,stable,healthy,environmentally friendly,energy-saving and income-increasing approach for sustainable development of yellow catfish breeding industry.
文摘为了提高中度嗜酸放线菌YY21产纤溶酶的产量,本文通过单因素实验对其液体发酵培养基和培养条件进行了研究。结果表明:发酵培养基组成为小米粉1.7%,葡萄糖2%,碳酸钙0.2%,氯化钠0.5%,蛋白胨0.6%;发酵条件为250 m L三角瓶装培养基50 m L,接种量5%,在22℃、转速160 r/min的条件下振荡培养4 d。在最适条件下,放线菌YY21液体发酵产物在血纤维蛋白平板上形成溶圈面积可达到164.38 mm^2,较初始产酶活力(溶圈面积87.58 mm^2)提高了88%,发酵时间缩短了3 d。通过对液体发酵培养基和培养条件的研究有效地提高了中度嗜酸放线菌YY21产纤溶酶的产量。
