Aim： To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues （blood and sperm） of different embryological origin. Methods： The present study included 52 infertile oligozoospermic...Aim： To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues （blood and sperm） of different embryological origin. Methods： The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction （PCR） microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker. Results： Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA. Conclusion： The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques （ART）, Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.展开更多
研究对L. casei YQ336进行高密度培养,通过单因素和响应面试验优化L. casei YQ336培养基和培养条件,绘制48 h内生长曲线。结果显示,L.casei YQ336最佳培养基组成为红糖20 g、酵母浸粉5 g、磷酸氢二钾5 g、绿豆∶黄豆∶甘薯为2∶1∶1的...研究对L. casei YQ336进行高密度培养,通过单因素和响应面试验优化L. casei YQ336培养基和培养条件,绘制48 h内生长曲线。结果显示,L.casei YQ336最佳培养基组成为红糖20 g、酵母浸粉5 g、磷酸氢二钾5 g、绿豆∶黄豆∶甘薯为2∶1∶1的增殖液1 000 mL。高密度培养L. casei YQ336的最优培养条件为接种量7%、培养温度35℃、初始pH值5.5、培养时间20 h。在该发酵条件下,L. casei YQ336发酵后活菌数达1.58×1010CFU/mL。展开更多
文摘Aim： To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues （blood and sperm） of different embryological origin. Methods： The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction （PCR） microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker. Results： Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA. Conclusion： The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques （ART）, Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.
文摘研究对L. casei YQ336进行高密度培养,通过单因素和响应面试验优化L. casei YQ336培养基和培养条件,绘制48 h内生长曲线。结果显示,L.casei YQ336最佳培养基组成为红糖20 g、酵母浸粉5 g、磷酸氢二钾5 g、绿豆∶黄豆∶甘薯为2∶1∶1的增殖液1 000 mL。高密度培养L. casei YQ336的最优培养条件为接种量7%、培养温度35℃、初始pH值5.5、培养时间20 h。在该发酵条件下,L. casei YQ336发酵后活菌数达1.58×1010CFU/mL。
