To identify milk-derived peptides with both antioxidant and calcium absorption activities in combating osteoporosis,we employed a comprehensive screening approach that included virtual enzymatic hydrolysis,molecular d...To identify milk-derived peptides with both antioxidant and calcium absorption activities in combating osteoporosis,we employed a comprehensive screening approach that included virtual enzymatic hydrolysis,molecular docking,and cellular experiments using osteoblasts.Under the optimal conditions for dual-enzyme hydrolysis,the 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical scavenging rate and soluble calcium binding capacity of the milk-derived peptides were 19.69% and 0.6965μg/mL,respectively.Six peptide segments,namely KEDVPSER,HKEMPFPK,YPSYG,EDVPSE,VPQLE,and IPAVF,were identified through UPLC-Q-Exactive Orbitrap MS and molecular docking for further validation.Among the peptides,YPSYG significantly promoted the proliferation of MC3T3-E1 cells both with and without CaCl_(2)(P<0.05),increasing proliferation by 38.27% and 20.67%,respectively,compared to the control group.Additionally,YPSYG significantly improved proliferation after H_(2)O_(2)-induced oxidative damage(P<0.05),with a 38.23%higher rate than the model group.Compared with rats in the osteoporosis model group,YPSYG significantly enhanced serum alkaline phosphatase(ALP)and N-terminal propeptide of type I procollagen in rats(s-PINP)levels and decreased tartrate-resistant acid phosphatase(TRAP)levels(P<0.05).Furthermore,milk-derived peptides and YPSYG significantly increased the bone weight index,maximum load,and bending energy of the femur and tibia in osteoporotic rats(P<0.05).Additionally,these peptides significantly reduced the number of osteoclasts in the metaphysis of the femur and tibia in osteoporotic rats and alleviated microstructural damage.This study confirmed that milk-derived peptides,including YPSYG,effectively promoted bone formation and improved bone microstructure in osteoporotic rats.These findings provided a foundation for developing functional foods for elderly bone health.展开更多
基金financially supported by Chinese Nutrition Society(CNS)Nutrition Science Foundation-Feihe Tizhi,nutrition and health research fund(CNS-Feihe2021-38).
文摘To identify milk-derived peptides with both antioxidant and calcium absorption activities in combating osteoporosis,we employed a comprehensive screening approach that included virtual enzymatic hydrolysis,molecular docking,and cellular experiments using osteoblasts.Under the optimal conditions for dual-enzyme hydrolysis,the 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical scavenging rate and soluble calcium binding capacity of the milk-derived peptides were 19.69% and 0.6965μg/mL,respectively.Six peptide segments,namely KEDVPSER,HKEMPFPK,YPSYG,EDVPSE,VPQLE,and IPAVF,were identified through UPLC-Q-Exactive Orbitrap MS and molecular docking for further validation.Among the peptides,YPSYG significantly promoted the proliferation of MC3T3-E1 cells both with and without CaCl_(2)(P<0.05),increasing proliferation by 38.27% and 20.67%,respectively,compared to the control group.Additionally,YPSYG significantly improved proliferation after H_(2)O_(2)-induced oxidative damage(P<0.05),with a 38.23%higher rate than the model group.Compared with rats in the osteoporosis model group,YPSYG significantly enhanced serum alkaline phosphatase(ALP)and N-terminal propeptide of type I procollagen in rats(s-PINP)levels and decreased tartrate-resistant acid phosphatase(TRAP)levels(P<0.05).Furthermore,milk-derived peptides and YPSYG significantly increased the bone weight index,maximum load,and bending energy of the femur and tibia in osteoporotic rats(P<0.05).Additionally,these peptides significantly reduced the number of osteoclasts in the metaphysis of the femur and tibia in osteoporotic rats and alleviated microstructural damage.This study confirmed that milk-derived peptides,including YPSYG,effectively promoted bone formation and improved bone microstructure in osteoporotic rats.These findings provided a foundation for developing functional foods for elderly bone health.
