[Objective] This study aimed to investigate the biological characteristics of a Bacillus thuringiensis strain YNI-1, which has high virulence to Lepidoptera spp. [Method] The crystal protein of YNI-1 was analyzed by S...[Objective] This study aimed to investigate the biological characteristics of a Bacillus thuringiensis strain YNI-1, which has high virulence to Lepidoptera spp. [Method] The crystal protein of YNI-1 was analyzed by SDS-PAGE, and its indoor and field efficacy for Lepidoptera spp. was investigated. [Result] The parasporal crystal of YNI-1 has a diamond-like structure. The molecular weight of the original toxin protein is 136 kDa. After trypsin treatment, the original toxin protein was hy- drolyzed into active toxin protein with molecular weight of 63 kDa. For Plutella xy- Iostella and Pieris rapae, the indoor efficacy of B. thuringiensis was better than that of commercial B. thuringiensis （WP）. In view of field efficacy, rate of YNI-1 strain was higher than that of commercial B [Conelusion] YNI-1 strain has excellent development potential. the insects reduced thuringiensis （WP）.展开更多
基金Supported by Natural Science Foundation of Yanbian University(2011-34)~~
文摘[Objective] This study aimed to investigate the biological characteristics of a Bacillus thuringiensis strain YNI-1, which has high virulence to Lepidoptera spp. [Method] The crystal protein of YNI-1 was analyzed by SDS-PAGE, and its indoor and field efficacy for Lepidoptera spp. was investigated. [Result] The parasporal crystal of YNI-1 has a diamond-like structure. The molecular weight of the original toxin protein is 136 kDa. After trypsin treatment, the original toxin protein was hy- drolyzed into active toxin protein with molecular weight of 63 kDa. For Plutella xy- Iostella and Pieris rapae, the indoor efficacy of B. thuringiensis was better than that of commercial B. thuringiensis （WP）. In view of field efficacy, rate of YNI-1 strain was higher than that of commercial B [Conelusion] YNI-1 strain has excellent development potential. the insects reduced thuringiensis （WP）.
