通过不同质量分数的磷酸三钠溶液震荡浸泡烟草种子30 m in,对种子的出苗和大田烟株TMV的发病情况进行调查统计.结果表明:1)30%和40%的磷酸三钠溶液对增强种子的活力有明显效果,播种后14 d出苗率分别达到63.5%和69.2%,分别比清水对照提高...通过不同质量分数的磷酸三钠溶液震荡浸泡烟草种子30 m in,对种子的出苗和大田烟株TMV的发病情况进行调查统计.结果表明:1)30%和40%的磷酸三钠溶液对增强种子的活力有明显效果,播种后14 d出苗率分别达到63.5%和69.2%,分别比清水对照提高了13.9%和19.6%;2)30%和40%的磷酸三钠溶液处理种子30 m in,可使大田烟株对TMV有显著的抑制效果,与其它处理的差异显著水平达到0.01;3)大田期烟株TMV的病情变化趋势,几乎不受磷酸三钠溶液处理的影响,而主要与降雨等气候因素关系密切.展开更多
Glioblastoma multiforme(GBM)is the deadliest form of brain tumor,and effective treatments are lacking.Thus,a new generation of effective treatments is urgently needed.B-cell lymphoma 6(BCL6)is a transcription factor t...Glioblastoma multiforme(GBM)is the deadliest form of brain tumor,and effective treatments are lacking.Thus,a new generation of effective treatments is urgently needed.B-cell lymphoma 6(BCL6)is a transcription factor that functions to suppress the transcription of DNA damage response genes,halting cell death in response to DNA damage.Here,we identified BCL6 as a lynchpin in GBM,the expression of which was greater in GBM cells than in normal cells and associated with poor survival in GBM patients.The silencing of BCL6 additionally affected GBM cell proliferation and triggered cellular damage.Furthermore,we reported the identification of YK01,a novel small-molecule inhibitor of BCL6.YK01 exhibited excellent anti-GBM bioactivity and caused apoptosis;importantly,YK01 significantly inhibited the growth of GBM cells both in vitro and in vivo.Moreover,the combination of YK01 and temozolomide treatment significantly suppressed the growth and metastasis of tumors in vivo and prolonged the survival of mice with tumors.In summary,our findings reveal that BCL6 appears to play a crucial role in GBM and may be a therapeutic target for treating this incurable condition.展开更多
文摘通过不同质量分数的磷酸三钠溶液震荡浸泡烟草种子30 m in,对种子的出苗和大田烟株TMV的发病情况进行调查统计.结果表明:1)30%和40%的磷酸三钠溶液对增强种子的活力有明显效果,播种后14 d出苗率分别达到63.5%和69.2%,分别比清水对照提高了13.9%和19.6%;2)30%和40%的磷酸三钠溶液处理种子30 m in,可使大田烟株对TMV有显著的抑制效果,与其它处理的差异显著水平达到0.01;3)大田期烟株TMV的病情变化趋势,几乎不受磷酸三钠溶液处理的影响,而主要与降雨等气候因素关系密切.
基金supported by the grants from the National Natural Science Foundation of China(No.82073310,82373146,82202897)The Jointed PI Program from Shanghai Changning Maternity and Infant Health Hospital(China)(No.PI202430)+3 种基金Shanghai Rising-Star Program(No.23QB1405600)The Science and Technology Commission of Shanghai Municipality,China(No.22QB1405600)Biomedical Projects of Yunnan Key Science and Technology Program(202502AA310002)ECNU(East China Normal University)Construction Fund of Innovation and Entrepreneurship Laboratory(Shanghai,China)(No.44400-20201-532300/021).
文摘Glioblastoma multiforme(GBM)is the deadliest form of brain tumor,and effective treatments are lacking.Thus,a new generation of effective treatments is urgently needed.B-cell lymphoma 6(BCL6)is a transcription factor that functions to suppress the transcription of DNA damage response genes,halting cell death in response to DNA damage.Here,we identified BCL6 as a lynchpin in GBM,the expression of which was greater in GBM cells than in normal cells and associated with poor survival in GBM patients.The silencing of BCL6 additionally affected GBM cell proliferation and triggered cellular damage.Furthermore,we reported the identification of YK01,a novel small-molecule inhibitor of BCL6.YK01 exhibited excellent anti-GBM bioactivity and caused apoptosis;importantly,YK01 significantly inhibited the growth of GBM cells both in vitro and in vivo.Moreover,the combination of YK01 and temozolomide treatment significantly suppressed the growth and metastasis of tumors in vivo and prolonged the survival of mice with tumors.In summary,our findings reveal that BCL6 appears to play a crucial role in GBM and may be a therapeutic target for treating this incurable condition.
