Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the productio...Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the production of chiralγ-decalactone.In this study,a green biphasic fermentation system containing 1-butyl-3-methylimidazo-lium tetrafluoroborate([C_(4)MIM]PF6)was developed to increase the synthesis of(R)-γ-decalactone from rici-noleic acid catalyzed by Clavispora lusitaniae YJ26.Under optimal conditions,the titer ofγ-decalactone obtained using the biphasic system reached 2.04 g/L,which is 2.4 times that of the traditional aqueous fermentation system,and showed high enantioselectivity(e.e.>99.0%).Fluorescence microscopy and flow cytometry results demonstrated that the addition of[C_(4)MIM]PF6 significantly alleviated the damage of substrate and product to cell membrane and improved cell viability,thus increasing the titer ofγ-decalactone.Furthermore,comparative transcriptomic analysis results suggested that the improved mechanism may be attributed to the up-regulation of genes associated with cell membrane component synthesis or cell tolerance enhancement.Overall,the experi-mental results provide valuable insights into the efficient microbial production of chiral decalactone in an ionic liquids-containing biphasic fermentation system.展开更多
基金supported by the Key Realm R&D Program of Guang-Dong Province(2022B0202050003)Guangdong Basic and Applied Basic Research Foundation(2022A1515110710)+1 种基金Science and Technology Projects in Guangzhou(2023A04J0745)Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology(2021B1212040013).
文摘Microbial production of chiralγ-decalactone has received considerable attention due to its high market value.However,the inhibition of substrates and products on microorganisms has significantly limited the production of chiralγ-decalactone.In this study,a green biphasic fermentation system containing 1-butyl-3-methylimidazo-lium tetrafluoroborate([C_(4)MIM]PF6)was developed to increase the synthesis of(R)-γ-decalactone from rici-noleic acid catalyzed by Clavispora lusitaniae YJ26.Under optimal conditions,the titer ofγ-decalactone obtained using the biphasic system reached 2.04 g/L,which is 2.4 times that of the traditional aqueous fermentation system,and showed high enantioselectivity(e.e.>99.0%).Fluorescence microscopy and flow cytometry results demonstrated that the addition of[C_(4)MIM]PF6 significantly alleviated the damage of substrate and product to cell membrane and improved cell viability,thus increasing the titer ofγ-decalactone.Furthermore,comparative transcriptomic analysis results suggested that the improved mechanism may be attributed to the up-regulation of genes associated with cell membrane component synthesis or cell tolerance enhancement.Overall,the experi-mental results provide valuable insights into the efficient microbial production of chiral decalactone in an ionic liquids-containing biphasic fermentation system.
