Dr. Yeh-chong Chan （Y.C. Chan） is one of the earliest acupuncturists in the United States （US）. He served for seven years in the first acupuncture center in the US, which was established in Washington, D.C. in 197...Dr. Yeh-chong Chan （Y.C. Chan） is one of the earliest acupuncturists in the United States （US）. He served for seven years in the first acupuncture center in the US, which was established in Washington, D.C. in 1972. In 1979, he moved the clinic to Rockville, Maryland and continued to practice acupuncture there for over 30 years. He is a well-known licensed acupuncturist （LAc）, one of the developers of the acupuncture profession, and a scholar of acupuncture and traditional Chinese medicine （TCM）. He has treated two US Governors （In the US, the title Governor refers to the chief executive of each state or insular territory and the political and ceremonial head of the state.） and many sports stars. He is the author of books entitled Acupuncture Practice in the United States,展开更多
[Objectives]This study aimed to establish a quality evaluation method for Herba Cistanche based on the HPLC characteristic chromatograms of the phenylethanoid glycosides and iridoid glycosides,and compare the HPLC cha...[Objectives]This study aimed to establish a quality evaluation method for Herba Cistanche based on the HPLC characteristic chromatograms of the phenylethanoid glycosides and iridoid glycosides,and compare the HPLC characteristic chromatograms of different origins of Herba Cistanche.[Methods]The chromatographic conditions used were as follows:column,Agilent ZORBAX Eclipse Plus C18 column(4.6 mm×250 mm,5μm);gradient elution,acetonitrile-0.1%phosphoric acid aqueous solution;flow rate,1.0 mL/min;detection wavelength,240 nm;column temperature,30℃.[Results]Cistanche tubulosa(Schenk)Wight showed 9 characteristic peaks,Cistanche deserticola Y.C.Ma showed 10 characteristic peaks,Cistanche salsa(C.A.Mey.)G.Beck showed 7 characteristic peaks,and Cistanche sinensis G.Beck showed 4 characteristic peaks.Peak 6 was echinacoside,peak 7 was verbascoside,peak 8 was tubuloside A,peak 9 was isoacteoside and peak 10 was cistanoside A.The characteristic chromatograms of the four different origins of Herba Cistanche were significantly different.[Conclusions]This characteristic chromatogram method has good reproducibility and can be used to distinguish 4 different origins of Herba Cistanche,C.deserticola Y.C.Ma,C.tubulosa(Schenk)Wight,C.sinensis G.Beck and C.salsa(C.A.Mey.)G.Beck.展开更多
Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性...Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性组织。Williams 是伦敦一家布店店员,后来成为店主。起初该组织是一个俱乐部,拥有12名青年成员,他们提出的口号是:“提高布店和其他行业青年的灵性修养”。这个俱乐部迅速推广到英国各地。1850年传到澳大利亚,1851年传到北美洲,其后在北美洲各地得到空前发展。展开更多
Objective: Gentamicin(GM) is a commonly used aminoglycoside antibiotic, however, renal toxicity has limited its usage. The present study was designed to evaluate the ameliorative effect of Cistanche deserticola on GM-...Objective: Gentamicin(GM) is a commonly used aminoglycoside antibiotic, however, renal toxicity has limited its usage. The present study was designed to evaluate the ameliorative effect of Cistanche deserticola on GM-induced nephrotoxicity in rats.Methods: The nephrotoxicity in rats was induced by intraperitoneal administration of GM(100 mg/kg)for 10 consecutive days. Glomerular filtration rate, blood urea nitrogen, creatinine and kidney histopathology were detected to assess the GM-induced nephrotoxicity. The oxidative stress(catalase,superoxide dismutase, glutathione and malondialdehyde) was assessed. The inflammatory response(tumor necrosis factor-a, interleukin-6, myeloperoxidase and nuclear factor-kappa B) and apoptotic marker(Bax and Bcl-2) were also evaluated.Results: The results showed that water and 75% ethanol extracts of C. deserticola(named CDW and CDE,respectively)(100, 200 and 400 mg/kg) in combination with GM could recover the reduction of glomerular filtration rate and enhance the renal endogenous antioxidant capability induced by GM. The increase in the expression of renal inflammatory cytokines(tumor necrosis factor-a and interleukin-6), nuclear protein of nuclear factor-kappa B(p65) and the activity of myeloperoxidase induced by GM was significantly decreased upon CDW or CDE treatment. In addition, CDW or CDE treatment could decrease the Bax protein expression and increase the Bcl-2 protein expression in GM-induced nephrotoxicity in rats significantly.Conclusion: The study demonstrated that C. deserticola treatment could attenuate kidney dysfunction and structural damage in rats induced by GM through the reduction of inflammation, oxidative stress and apoptosis.展开更多
Objective: Cistanche deserticola is a famous and endangered medicinal plant that is parasitic upon Haloxylon ammodendron with rather low parasitic rates. It is important to find high affinity germplasms for increasing...Objective: Cistanche deserticola is a famous and endangered medicinal plant that is parasitic upon Haloxylon ammodendron with rather low parasitic rates. It is important to find high affinity germplasms for increasing the survival of C. deserticola. However, little is known in genetic variation and high affinity populations of H. ammodendron in China.Methods: In this study, 98 accessions of H. ammodendron seeds were collected from five regions covering almost the entire natural distribution of H. ammodendron in China. Their genetic variations were analyzed using AFLP and ITS by the maximum parsimony method, and a dendrogram was constructed using the unweighted pair-group method with arithmetic average(UPGMA). The parasitic rates of C. deserticola on different accessions of H. ammodendron were calculated in the field experiment.Results: Both AFLP and ITS methods consistently revealed that there was a high level of genetic diversity in the natural populations of H. ammodendron. Hierarchical population structure analysis uncovered a clear pattern that all populations were grouped into three main clusters, and eight populations from eastern region were genetically clustered together. These regions were significantly differentiated(P < 0.05), 13.10% of variation occurred among populations, and 86.90% within populations was revealed by analysis of molecular variance(AMOVA). The populations of Inner Mongolia had the highest parasitic rates followed by Ganjiahu Reserve and Yongning Plantation for the top three, which were not completely related to the genetic variation.Conclusion: Genetic characteristics of H. ammodendron in China were clarified and the order of affinity of different populations was given, which were primers for discovering high affinity germplasms.展开更多
文摘Dr. Yeh-chong Chan （Y.C. Chan） is one of the earliest acupuncturists in the United States （US）. He served for seven years in the first acupuncture center in the US, which was established in Washington, D.C. in 1972. In 1979, he moved the clinic to Rockville, Maryland and continued to practice acupuncture there for over 30 years. He is a well-known licensed acupuncturist （LAc）, one of the developers of the acupuncture profession, and a scholar of acupuncture and traditional Chinese medicine （TCM）. He has treated two US Governors （In the US, the title Governor refers to the chief executive of each state or insular territory and the political and ceremonial head of the state.） and many sports stars. He is the author of books entitled Acupuncture Practice in the United States,
基金Nanning Excellent Young Scientist Program(RC20180206).
文摘[Objectives]This study aimed to establish a quality evaluation method for Herba Cistanche based on the HPLC characteristic chromatograms of the phenylethanoid glycosides and iridoid glycosides,and compare the HPLC characteristic chromatograms of different origins of Herba Cistanche.[Methods]The chromatographic conditions used were as follows:column,Agilent ZORBAX Eclipse Plus C18 column(4.6 mm×250 mm,5μm);gradient elution,acetonitrile-0.1%phosphoric acid aqueous solution;flow rate,1.0 mL/min;detection wavelength,240 nm;column temperature,30℃.[Results]Cistanche tubulosa(Schenk)Wight showed 9 characteristic peaks,Cistanche deserticola Y.C.Ma showed 10 characteristic peaks,Cistanche salsa(C.A.Mey.)G.Beck showed 7 characteristic peaks,and Cistanche sinensis G.Beck showed 4 characteristic peaks.Peak 6 was echinacoside,peak 7 was verbascoside,peak 8 was tubuloside A,peak 9 was isoacteoside and peak 10 was cistanoside A.The characteristic chromatograms of the four different origins of Herba Cistanche were significantly different.[Conclusions]This characteristic chromatogram method has good reproducibility and can be used to distinguish 4 different origins of Herba Cistanche,C.deserticola Y.C.Ma,C.tubulosa(Schenk)Wight,C.sinensis G.Beck and C.salsa(C.A.Mey.)G.Beck.
文摘Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性组织。Williams 是伦敦一家布店店员,后来成为店主。起初该组织是一个俱乐部,拥有12名青年成员,他们提出的口号是:“提高布店和其他行业青年的灵性修养”。这个俱乐部迅速推广到英国各地。1850年传到澳大利亚,1851年传到北美洲,其后在北美洲各地得到空前发展。
基金supported by the National Key Research and Development Program of China (2018YFC1706300, 2018YFC17063003)the Key Research and Development Project of Gan Su Province (20YF2FA019)+2 种基金the People’s Livelihood Special Project from Technical Innovation Guide Plan of Gansu Province (20CX4FK014)the Key Talent Project of Organization Department of Gansu Provincial Party Committee ([2022]Gan-71)the Talent Innovation and Entrepreneurship Project of Lanzhou (2017-RC115, 2020-RC-41)。
文摘Objective: Gentamicin(GM) is a commonly used aminoglycoside antibiotic, however, renal toxicity has limited its usage. The present study was designed to evaluate the ameliorative effect of Cistanche deserticola on GM-induced nephrotoxicity in rats.Methods: The nephrotoxicity in rats was induced by intraperitoneal administration of GM(100 mg/kg)for 10 consecutive days. Glomerular filtration rate, blood urea nitrogen, creatinine and kidney histopathology were detected to assess the GM-induced nephrotoxicity. The oxidative stress(catalase,superoxide dismutase, glutathione and malondialdehyde) was assessed. The inflammatory response(tumor necrosis factor-a, interleukin-6, myeloperoxidase and nuclear factor-kappa B) and apoptotic marker(Bax and Bcl-2) were also evaluated.Results: The results showed that water and 75% ethanol extracts of C. deserticola(named CDW and CDE,respectively)(100, 200 and 400 mg/kg) in combination with GM could recover the reduction of glomerular filtration rate and enhance the renal endogenous antioxidant capability induced by GM. The increase in the expression of renal inflammatory cytokines(tumor necrosis factor-a and interleukin-6), nuclear protein of nuclear factor-kappa B(p65) and the activity of myeloperoxidase induced by GM was significantly decreased upon CDW or CDE treatment. In addition, CDW or CDE treatment could decrease the Bax protein expression and increase the Bcl-2 protein expression in GM-induced nephrotoxicity in rats significantly.Conclusion: The study demonstrated that C. deserticola treatment could attenuate kidney dysfunction and structural damage in rats induced by GM through the reduction of inflammation, oxidative stress and apoptosis.
基金National Natural Science Foundation of China (81773851 & U1403224)Fundamental Research Fundfor the Central Scientific Research Institutes for Public Welfare (YZ-12-09)CAMS Innovation Fundfor Medical Sciences (2016-I2M-3-017) for the financial provides
文摘Objective: Cistanche deserticola is a famous and endangered medicinal plant that is parasitic upon Haloxylon ammodendron with rather low parasitic rates. It is important to find high affinity germplasms for increasing the survival of C. deserticola. However, little is known in genetic variation and high affinity populations of H. ammodendron in China.Methods: In this study, 98 accessions of H. ammodendron seeds were collected from five regions covering almost the entire natural distribution of H. ammodendron in China. Their genetic variations were analyzed using AFLP and ITS by the maximum parsimony method, and a dendrogram was constructed using the unweighted pair-group method with arithmetic average(UPGMA). The parasitic rates of C. deserticola on different accessions of H. ammodendron were calculated in the field experiment.Results: Both AFLP and ITS methods consistently revealed that there was a high level of genetic diversity in the natural populations of H. ammodendron. Hierarchical population structure analysis uncovered a clear pattern that all populations were grouped into three main clusters, and eight populations from eastern region were genetically clustered together. These regions were significantly differentiated(P < 0.05), 13.10% of variation occurred among populations, and 86.90% within populations was revealed by analysis of molecular variance(AMOVA). The populations of Inner Mongolia had the highest parasitic rates followed by Ganjiahu Reserve and Yongning Plantation for the top three, which were not completely related to the genetic variation.Conclusion: Genetic characteristics of H. ammodendron in China were clarified and the order of affinity of different populations was given, which were primers for discovering high affinity germplasms.
