To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) wa...To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) was amplified from the recombinant plasmid pTG402 by using PCR technique and was subcloned into pUC118N and pUC119N. The single stranded recombinant phage DNA from the transformed E. coli MV1184 cells was used for sequencing. The sequence of xylE gene was proved to be the same as reported. The gene was then subcloned into the high expression plasmid pJLA503, its expression amount being about 34. 2% of the total bacterial proteins. Conclusion: xylE gene is highly expressed in host E. coli TG1.展开更多
The chemical industry is not only a pillar in-dustry of China’s national economy but also an important supplier of energy and raw materials.It is also an industry with high en-ergy consumption and hard-to-overcome po...The chemical industry is not only a pillar in-dustry of China’s national economy but also an important supplier of energy and raw materials.It is also an industry with high en-ergy consumption and hard-to-overcome pollution.At present there is no practical technology in China to treat many pollutants discharged by the dyestuffs,pesticides,fine chemicals and other chemical sectors.In the production of pesticide active展开更多
文摘To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) was amplified from the recombinant plasmid pTG402 by using PCR technique and was subcloned into pUC118N and pUC119N. The single stranded recombinant phage DNA from the transformed E. coli MV1184 cells was used for sequencing. The sequence of xylE gene was proved to be the same as reported. The gene was then subcloned into the high expression plasmid pJLA503, its expression amount being about 34. 2% of the total bacterial proteins. Conclusion: xylE gene is highly expressed in host E. coli TG1.
文摘The chemical industry is not only a pillar in-dustry of China’s national economy but also an important supplier of energy and raw materials.It is also an industry with high en-ergy consumption and hard-to-overcome pollution.At present there is no practical technology in China to treat many pollutants discharged by the dyestuffs,pesticides,fine chemicals and other chemical sectors.In the production of pesticide active
