[Objectives]To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule(XXC).[Methods]The chromatographic conditions for the analysis of XXC solution were as follows:XSelect HSS T3 column;acetonitrile-0.1%pho...[Objectives]To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule(XXC).[Methods]The chromatographic conditions for the analysis of XXC solution were as follows:XSelect HSS T3 column;acetonitrile-0.1%phosphoric acid water was used as mo-bile phase,gradient elution;flow rate:1.0 mL/min;column temperature 30℃;The injection volume is 10μL.The quality of XXC samples produced by different manufacturers was evaluated by similarity evaluation and cluster analysis.[Results]In theHPLC-dad fingerprints of 15 batches of XXC,23 common peaks were identified and 9 peaks were identified,and the similarity was greater than 0.95.According to the re-sults of cluster analysis,15 batches of XXC samples could be divided into two categories,S2,S5,S6,S7 and S8 batches belonged to category Ⅰ,and the rest batches belonged to category Ⅱ.[Conclusions]In this study,a representative and universal identification method of Xxc HPLC-DAD fingerprint was established.The method has high precision,stability and repeatability,is simple and reliable,and provides a pow-erful reference for further improving the quality evaluation system of XXC.展开更多
Background Retinal vein occlusion （RVO） is one of the most common causes of visual loss. Many approaches have been tried to treat central retinal vein occlusion （CRVO）, and branch retinal vein occlusion （BRVO） w...Background Retinal vein occlusion （RVO） is one of the most common causes of visual loss. Many approaches have been tried to treat central retinal vein occlusion （CRVO）, and branch retinal vein occlusion （BRVO） with various results. However, there is no defined protocol and limited evidence to support the interventions currently used. The aim of this study was to assess the efficacy of the traditional Chinese medicine Fufang XueShuan Tong （FXST） in treating experimentally created RVO. Methods RVO model was first induced in forty-four pigmented rabbits through photocoagulation following injection of rose Bengal. The rabbits were divided into four groups based on the dose of FXST administered （212 mg/kg, 424 mg/kg, 848 mg/kg and control group）. The rabbits were observed for four weeks after the procedure, using color fundus photography, fundus fluorescein angiography and electroretinogram examination. Vascular endothelial growth factor （VEGF）, interleukin-6 and nitric oxide （NO） levels in the vitreous and histopathologic evaluation were monitored. Results The obstructed vessels in the treatment groups reopened or anastomosed faster than those in the control group （P〈0.05）. The amplitude of maximum b wave and the oscillatory potential were significantly higher in the treatment groups than in the control group （P 〈0.01）. At both two weeks and four weeks, VEGF and IL-6 levels in the vitreous were significantly decreased in the treatment groups （P 〈0.01）, while NO levels were significantly elevated （P 〈0.01）. At the same time, histopathologic evaluation showed different retinal neuroepithelium structures in the different groups. Immunoreactivity of VEGF was greater in the control group than in the treatment groups. Conclusion FXST was helpful in reconstructing retinal vessels in the RVO model, protecting retinal structures and improvinq visual function, and could inhibit the neovascular factor.展开更多
文摘[Objectives]To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule(XXC).[Methods]The chromatographic conditions for the analysis of XXC solution were as follows:XSelect HSS T3 column;acetonitrile-0.1%phosphoric acid water was used as mo-bile phase,gradient elution;flow rate:1.0 mL/min;column temperature 30℃;The injection volume is 10μL.The quality of XXC samples produced by different manufacturers was evaluated by similarity evaluation and cluster analysis.[Results]In theHPLC-dad fingerprints of 15 batches of XXC,23 common peaks were identified and 9 peaks were identified,and the similarity was greater than 0.95.According to the re-sults of cluster analysis,15 batches of XXC samples could be divided into two categories,S2,S5,S6,S7 and S8 batches belonged to category Ⅰ,and the rest batches belonged to category Ⅱ.[Conclusions]In this study,a representative and universal identification method of Xxc HPLC-DAD fingerprint was established.The method has high precision,stability and repeatability,is simple and reliable,and provides a pow-erful reference for further improving the quality evaluation system of XXC.
文摘Background Retinal vein occlusion （RVO） is one of the most common causes of visual loss. Many approaches have been tried to treat central retinal vein occlusion （CRVO）, and branch retinal vein occlusion （BRVO） with various results. However, there is no defined protocol and limited evidence to support the interventions currently used. The aim of this study was to assess the efficacy of the traditional Chinese medicine Fufang XueShuan Tong （FXST） in treating experimentally created RVO. Methods RVO model was first induced in forty-four pigmented rabbits through photocoagulation following injection of rose Bengal. The rabbits were divided into four groups based on the dose of FXST administered （212 mg/kg, 424 mg/kg, 848 mg/kg and control group）. The rabbits were observed for four weeks after the procedure, using color fundus photography, fundus fluorescein angiography and electroretinogram examination. Vascular endothelial growth factor （VEGF）, interleukin-6 and nitric oxide （NO） levels in the vitreous and histopathologic evaluation were monitored. Results The obstructed vessels in the treatment groups reopened or anastomosed faster than those in the control group （P〈0.05）. The amplitude of maximum b wave and the oscillatory potential were significantly higher in the treatment groups than in the control group （P 〈0.01）. At both two weeks and four weeks, VEGF and IL-6 levels in the vitreous were significantly decreased in the treatment groups （P 〈0.01）, while NO levels were significantly elevated （P 〈0.01）. At the same time, histopathologic evaluation showed different retinal neuroepithelium structures in the different groups. Immunoreactivity of VEGF was greater in the control group than in the treatment groups. Conclusion FXST was helpful in reconstructing retinal vessels in the RVO model, protecting retinal structures and improvinq visual function, and could inhibit the neovascular factor.
