Objective:Xuebijing injection has been recommended as a therapeutic approach for individuals with severe and critical COVID-19.This study aims to explore the correlation of neutrophil to lymphocyte platelet ratio(NLPR...Objective:Xuebijing injection has been recommended as a therapeutic approach for individuals with severe and critical COVID-19.This study aims to explore the correlation of neutrophil to lymphocyte platelet ratio(NLPR)with the severity and prognosis of COVID-19,and the effect of XBJ on the prognosis of patients with COVID-19 in different inflammatory states.Methods:This was a retrospective study conducted at Wuhan Union Hospital in China.COVID-19patients admitted between November 1,2022 and February 1,2023 were included.In predicting prognosis for individuals with COVID-19,new inflammatory indicators were used,and their prognostic value was assessed by using Cox regression models and receiver operating characteristic curves.Furthermore,a calculation was made to determine the cutoff value for NLPR.Relative risk and Cox regression models were used to examine the effects of Xuebijing injection on prognosis in patient cohorts that had been stratified by the NLPR cutoff.Results:This research included 455 participants with COVID-19,with a mean age of 72 years.Several inflammatory indicators were found to be strongly correlated with prognosis,and NLPR shows the greatest predictive power.Patients with NLPR>3.29 exhibited a mortality rate of 17.3%,which was 6.2 times higher than in patients with NLPR≤3.29.Importantly,providing Xuebijing injection to patients with NLPR>3.29 was associated with a lower risk of 60-day all-cause mortality.However,there was no discernible improvement in survival among patients with NLPR≤3.29 who received Xuebijing injection.Conclusion:NLPR is the most reliable inflammatory marker for predicting prognosis among individuals with COVID-19,and can accurately identify individuals who may benefit from Xuebijing injection.展开更多
OBJECTIVE： To evaluate the effects of Xuebijing （XBJ） injection in heat stroke （HS） rats and to inves- tigate the mechanisms underlying these effects. METHODS： Sixty anesthetized rats were random- ized into thre...OBJECTIVE： To evaluate the effects of Xuebijing （XBJ） injection in heat stroke （HS） rats and to inves- tigate the mechanisms underlying these effects. METHODS： Sixty anesthetized rats were random- ized into three groups and intravenously injected twice daily for 3 days with 4 mL XBJ （XBJ group） or phosphate buffered saline （HS and Sham groups） per kg body weight. HS was initiated in the HS and XBJ groups by placing rats in a simulated climate chamber （ambient temperature 40℃：, humidity 60% ）. Rectal temperature, aterial pressure, and heart rate were monitored and recorded. Time to HS onset and survival were determined, and serum concentrations of tumor necrosis factor （TNF）-α, in-terleukin （IL）-1β, IL-6, alanine-aminotransferase （ALT）, and aspartate-aminotransferase （AST） were measured. Hepatic tissue was harvested for patho- logical examination and electron microscopic ex- amination. Kupffer cells （KCs） were separated from liver at HS initiation, and the concentrations of se- creted TNF-α, IL-1β3 and IL-6 were measured. RESULTS： Time to HS onset and survival were signif- icantly longer in the XBJ than in the HS group. Moreover, the concentrations of TNF-α, IL-1β, IL-6, ALT and AST were lower and liver injury was milder in the XBJ than in the HS group. Heat-stress in- duced structural changes in KCs and hepatic cells were more severe in the HS than in the XBJ group and the concentrations of TNF-α, IL-1β and IL-6 se- creted by KCs were lower in the XBJ than in the HS group. CONCLUSION： XBJ can alleviate HS-induced sys- temic inflammatory response syndrome and liver injury in rats, and improve outcomes. These protec- tive effects may be due to the ability of XBJ to inhib- it cytokine secretion by KCs.展开更多
Objective: To observe the effects of Xuebijing Injection (血必净注射液) in patients with severe pneumonia, and to explore the mechanism. Methods: Eighty cases of severe pneumonia are randomly assigned to the Xuebijing...Objective: To observe the effects of Xuebijing Injection (血必净注射液) in patients with severe pneumonia, and to explore the mechanism. Methods: Eighty cases of severe pneumonia are randomly assigned to the Xuebijing treatment (forty cases) and the control group (forty cases), with the same routine therapy provided in both groups. Clinical effective rates, inflammatory factors and organ function were observed in both groups. Results: The effective rate was higher in Xuebijing group than that of the control group (80.0% vs. 67.5%, P<0.05). As compared with the control group, the LDH, α1-AG, α1-AT levels and the peak body temperature decreased markedly with the Xuebijing treatment going, and the secretion of TNF-α, IL-6, IL-8 was suppressed in Xuebijing group; but no significant difference was found in leptin level. Conclusion: Xuebijing Injection may show a protective effect in patients with severe pneumonia. The mechanism is possibly with the decreased secretion of TNF-α, IL-6, and IL-8.展开更多
As a representative drug for the treatment of severe community-acquired pneumonia and sepsis,Xuebijing(XBJ)injection is also one of the recommended drugs for the prevention and treatment of coronavirus disease 2019(CO...As a representative drug for the treatment of severe community-acquired pneumonia and sepsis,Xuebijing(XBJ)injection is also one of the recommended drugs for the prevention and treatment of coronavirus disease 2019(COVID-19),but its treatment mechanism for COVID-19 is still unclear.Therefore,this study aims to explore the potential mechanism of XBJ injection in the treatment of COVID-19 employing network pharmacology and molecular docking methods.The corresponding target genes of 45 main active ingredients in XBJ injection and COVID-19 were obtained by using multiple database retrieval and literature mining.102 overlapping targets of them were screened as the core targets for analysis.Then built the PPI network,TCM-compound-target-disease,and disease-target-pathway networks with the help of Cytoscape 3.6.1 software.After that,utilized DAVID to perform gene ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to predict the action mechanism of overlapping targets.Finally,by applying molecular docking technology,all compounds were docked with COVID-193 CL protease(3 CLpro),spike protein(S protein),and angiotensin-converting enzyme II(ACE2).The results indicated that quercetin,luteolin,apigenin and other compounds in XBJ injection could affect TNF,MAPK1,IL6 and other overlapping targets.Meanwhile,anhydrosafflor yellow B(AHSYB),salvianolic acid B(SAB),and rutin could combine with COVID-19 crucial proteins,and then played the role of anti-inflammatory,antiviral and immune response to treat COVID-19.This study revealed the multiple active components,multiple targets,and multiple pathways of XBJ injection in the treatment of COVID-19,which provided a new perspective for the study of the mechanism of traditional Chinese medicine(TCM)in the treatment of COVID-19.展开更多
Acute lung injury/acute respiratory distress syndrome(ALI/ARDS)is characterized by diffuse alveolar injury primarily caused by an excessive inflammatory response.Regrettably,the lack of effective pharmacotherapy curre...Acute lung injury/acute respiratory distress syndrome(ALI/ARDS)is characterized by diffuse alveolar injury primarily caused by an excessive inflammatory response.Regrettably,the lack of effective pharmacotherapy currently available contributes to the high mortality rate in patients with this condition.Xuebijing(XBJ),a traditional Chinese medicine recognized for its potent anti-inflammatory properties,exhibits promise as a potential therapeutic agent for ALI/ARDS.This study aimed to explore the preventive effects of XBJ on ALI and its underlying mechanism.To this end,we established an LPS-induced ALI model and treated ALI mice with XBJ.Our results demonstrated that pre-treatment with XBJ significantly alleviated lung inflammation and increased the survival rate of ALI mice by 37.5%.Moreover,XBJ substantially suppressed the production of TNF-α,IL-6,and IL-1βin the lung tissue.Subsequently,we performed a network pharmacology analysis and identified identified 109 potential target genes of XBJ that were mainly involved in multiple signaling pathways related to programmed cell death and anti-inflammatory responses.Furthermore,we found that XBJ exerted its inhibitory effect on gasdermin-E-mediated pyroptosis of lung cells by suppressing TNF-αproduction.Therefore,this study not only establishes the preventive efficacy of XBJ in ALI but also reveals its role in protecting alveolar epithelial cells against gasdermin-E-mediated pyroptosis by reducing TNF-αrelease.展开更多
Severe pneumonia is one of the most common infectious diseases and the leading cause of sepsis and septic shock.Preventing infection,balancing the patient’s immune status,and anti-coagulation therapy are all importan...Severe pneumonia is one of the most common infectious diseases and the leading cause of sepsis and septic shock.Preventing infection,balancing the patient’s immune status,and anti-coagulation therapy are all important elements in the treatment of severe pneumonia.As multi-target agents,Xuebijing injection(XBJ)has shown unique advantages in targeting complex conditions and saving the lives of patients with severe pneumonia.This review outlines progress in the understanding of XBJ’s anti-inflammatory,endotoxin antagonism,and anticoagulation effects.From the hundreds of publications released over the past few years,the key results from representative clinical studies of XBJ in the treatment of severe pneumonia were selected and summarized.XBJ was observed to effectively suppress the release of pro-inflammatory cytokines,counter the effects of endotoxin,and assert an anticoagulation effect in most clinical trials,which are consistent with experimental studies.Collectively,this evidence suggests that XBJ could play an important and expanding role in clinical medicine,especially for sepsis,septic shock and severe pneumonia.展开更多
OBJECTIVE： Paraquat （PQ） poisoning-induced pul- monary fibrosis causes asphyxiation and death. The therapeutic potential of intravenous Xuebijing ther- apy in PQ poisoning patients and its underlying im- munomodula...OBJECTIVE： Paraquat （PQ） poisoning-induced pul- monary fibrosis causes asphyxiation and death. The therapeutic potential of intravenous Xuebijing ther- apy in PQ poisoning patients and its underlying im- munomodulatory effects on transforming growth factor （TGF）-131 and procollagen type III peptide （PIIIP） were investigated. METHODS： Thirty-six acute PQ poisoning patients were randomly assigned to conventional therapy （Group A） and intravenous Xuebijing administra- tion plus conventional therapy （Group 13）. Twenty volunteers served as controls （Group C）. Blood sam- ples were collected upon admission （day O） and at post-treatment days 5, 10, and 14. TGF-β1 and PIIIP concentrations were determined by ELISA and ana- lyzed for intra- and inter-group differences over time. One-month follow-up was conducted for de- termining the mortality rate. RESULTS： TGF-β1 and PIIIP levels were significantly higher in PQ poisoning patients and increased over time （Groups A and B vs C, P〈0.01）. However, the TGF-β1 and PIIIP levels were consistently significant- ly lower in Group B compared with those of Group A （P〈0.01）. The 1-month mortality rate was also lower in Group B compared with that of Group A （P〈0.05）. PQ poisoning patients showed remark- ably high levels of TGF-β1 and PIIIP, which increased as PQ-induced pulmonary fibrosis progressed. CONCLUSION： Treatment with intravenous Xuebi- jing plus conventional therapy significantly low- ered TGF-β1 and PIIIP levels, which indicates thera- peutic efficacy in the treatment of PQ poisoning pa- tients.展开更多
OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myo...OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myocardial injury were recruited, and were randomly divided into two groups: treatment group and control group. All patients in two groups received conventional cluster treatment, the patients in treatment group additional received Xuebijing injection dissolved in0.9% sodium chloride injection, and the patients in control group received the same amount of 0.9%sodium chloride injection. At the beginning of treatment and 3, 7 and 10-day after treatment, lab-oratory indicators of cardiac troponin Ⅰ(cTnI),N-terminal pro B-type natriuretic peptide(NT-pro BNP) and procalcitonin(PCT) were respectively tested in venous blood. The patient's length of stay in Intensive Care Unit(ICU) and the mortality in 28 days were recorded.RESULTS: At 3, 7 and 10-day after treatment, the improvements of c Tn I, NT-pro BNP and PCT in treatment group were better than those in control group, and the differences were statistically significant(P < 0.05). The mortality of treatment group in28 days was not significantly different from that of control group(P > 0.05). The ICU length of stay of treatment group was shorter than that of control group(P > 0.05).CONCLUSION: Xuebijing injection could improve the levels of c Tn I, NT-pro BNP and PCT in patients with septic myocardial injury.and it had a protective effect on myocardial injury.展开更多
XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based ass...XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based assay for quality evaluation of XueBiJing.Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel.Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition,pharmacokinetics,and pharmacodynamics.A selectivity test(for“similarity of response”)was developed to identify and minimize interference by nontarget constituents.Then,an intercept test was developed to fulfill“linearity through zero”for each analyte(absolute ratio of intercept to C response,<2%).Using the newly developed assays,we analyzed samples from 33 batches of XueBiJing,manufactured over three years,and found small batch-to-batch variability in contents of the marker constituents(4.1%-14.8%),except for senkyunolide I(26.5%).展开更多
BACKGROUND:Xuebijing(XBJ)can alleviate the inflammatory response,improve organ function,and shorten the intensive care unit(ICU)stay in patients with pyogenic liver abscess(PLA)complicated with sepsis,but the molecula...BACKGROUND:Xuebijing(XBJ)can alleviate the inflammatory response,improve organ function,and shorten the intensive care unit(ICU)stay in patients with pyogenic liver abscess(PLA)complicated with sepsis,but the molecular mechanisms have not been elucidated.This study aimed to explore the molecular mechanism of XBJ in treating PLA complicated with sepsis using a network pharmacology approach.METHODS:The active ingredients and targets of XBJ were retrieved from the ETCM database.Potential targets related to PLA and sepsis were retrieved from the GeneCards,PharmGKB,DisGeNet,Online Mendelian Inheritance in Man(OMIM),Therapeutic Targets Database(TTD),and DrugBank databases.The targets of PLA complicated with sepsis were mapped to the targets of XBJ to identify potential treatment targets.Protein-protein interaction networks were analyzed using the STRING database.Potential treatment targets were imported into the Metascape platform for Gene Ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.Molecular docking was performed to validate the interactions between active ingredients and core targets.RESULTS:XBJ was found to have 54 potential treatment targets for PLA complicated with sepsis.Interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor(TNF)were identifi ed as core targets.KEGG enrichment analysis revealed important pathways,including the interleukin-17(IL-17)signaling pathway,the TNF signaling pathway,the nuclear factor-kappa B(NF-κB)signaling pathway,and the Toll-like receptor(TLR)signaling pathway.Molecular docking experiments indicated stable binding between XBJ active ingredients and core targets.CONCLUSION:XBJ may exert therapeutic eff ects on PLA complicated with sepsis by modulating signaling pathways,such as the IL-17,TNF,NF-κB,and TLR pathways,and targeting IL-1β,IL-6,and TNF.展开更多
BACKGROUND Cardiopulmonary bypass(CPB)is an essential procedure for maintaining the blood supply to vital organs in patients undergoing cardiac surgery.However,perioperative cardiac injury related to CPB remains a sev...BACKGROUND Cardiopulmonary bypass(CPB)is an essential procedure for maintaining the blood supply to vital organs in patients undergoing cardiac surgery.However,perioperative cardiac injury related to CPB remains a severe complication in these patients.Cardiac protection is important for patients undergoing CPB.AIM To evaluate the potential cardioprotective efficacy of the Chinese medicine preparation Xuebijing injection(XBJ)in patients undergoing CPB.METHODS Sixty patients undergoing cardiac surgery with CPB were randomly allocated to the XBJ and control groups(saline).XBJ was administered intravenously three times:12 h prior to surgery,at the beginning of the surgery,and 12 h after the second injection.Cardiac function was evaluated by echocardiography 48 h after surgery.Circulating inflammation-and oxidative-stress-related markers were measured.Clinical outcomes related to intensive care unit(ICU)stay were recorded.RESULTS Compared to control treatment,XBJ was associated with improved PaO2/FiO2 and cardiac systolic function,but reduced troponin I and creatine kinase fraction after surgery(all P<0.05).The circulating concentrations of tumor necrosis factor-α,interleukin(IL)-1βand IL-8 in the XBJ group were significantly lower than those in the control group(all P<0.05),whereas the circulating concentration of IL-10 was significantly higher in the XBJ group(P<0.05).In addition,the lengths of ICU stay and hospitalization after surgery tended to be shorter in the XBJ group than in the control group,although the differences were not significant.CONCLUSION Perioperative administration of XBJ was associated with attenuated cardiac injury during CPB,likely via anti-inflammatory and antioxidative mechanisms.展开更多
BACKGROUND: The study aimed to investigate the therapeutic benefits of intravenous Xuebijing on acute kidney injury(AKI) in rats with paraquat intoxication.METHODS: Male Sprague-Dawley rats were randomly divided equal...BACKGROUND: The study aimed to investigate the therapeutic benefits of intravenous Xuebijing on acute kidney injury(AKI) in rats with paraquat intoxication.METHODS: Male Sprague-Dawley rats were randomly divided equally into three groups: sham group(n=8), paraquat group(n=8) and Xuebijing-treated group(n=8) using a random number table. The rats were intraperitoneally injected with 50 mg/kg of paraquat. One hour after paraquat administration, the rats were treated intravenously with Xuebijing(8 mL /kg). At 12 hours after paraquat administration, serum was collected to evaluate kidney function, then the rats were sacrificed and kidney samples were immediately harvested. AKI scores were evaluated by renal histopathology and pro-in? ammatory cytokines mR NA levels in kidney were assayed using real-time RT-PCR.RESULTS: Serum urea nitrogen, creatinine and AKI scores were significantly higher in the paraquat group, compared with the sham group(P<0.05, respectively). Moreover, interleukin(IL)-1β, IL-6 and TNF-α m RNA levels were signi? cantly higher in the paraquat group(P<0.01, respectively). However, intravenous Xuebijing signi? cantly decreased serum urea nitrogen, creatinine, AKI scores and IL-1β, IL-6 and TNF-α m RNA levels, compared with the paraquat group(P<0.05, respectively).CONCLUSION: Intravenous Xuebijing attenuates AKI following paraquat poisoning by suppressing in? ammatory response.展开更多
Background:Gastrointestinal(GI)injury is one of the most common side effects of radiotherapy.However,there is no ideal therapy method except for symptomatic treatment in the clinic.Xuebijing(XBJ)is a traditional Chine...Background:Gastrointestinal(GI)injury is one of the most common side effects of radiotherapy.However,there is no ideal therapy method except for symptomatic treatment in the clinic.Xuebijing(XBJ)is a traditional Chinese medicine,used to treat sepsis by injection.In this study,the protective effects of XBJ on radiation-i nduced intestinal injury(RⅢ)and its mechanism were explored.Methods:The effect of XBJ on survival of irradiated C57BL/6 mice was monitored.Histological changes including the number of crypts and the length of villi were evaluated by H&E.The expression of Lgr5^(+)intestinal stem cells(ISCs),Ki67^(+)cells,villin and lysozymes were examined by immunohistochemistry.The expression of cytokines in the intestinal crypt was detected by RT-PCR.DNA damage and apoptosis rates in the small intestine were also evaluated by immunofluorescence.Results:In the present study,XBJ improved the survival rate of the mice after 8.0and 9.0 Gy total body irradiation(TBI).XBJ attenuated structural damage of the small intestine,maintained regenerative ability and promoted proliferation and differentiation of crypt cells,decreased apoptosis rate and reduced DNA damage in the intestine.Elevation of IL-6 and TNF-α was limited,but IL-1,TNF-β and IL-10 levels were increased in XBJ-treated group after irradiation.The expression of Bax and p53 were decreased after XBJ treatment.Conclusions:Taken together,XBJ provides a protective effect on RⅢby inhibiting inflammation and blocking p53-related apoptosis pathway.展开更多
We established a rat model of cardiac arrest by clamping the endotracheal tube of adult rats at expiration. Twenty-four hours after cardiopulmonary resuscitation, nerve cell injury and expression of tumor necrosis fac...We established a rat model of cardiac arrest by clamping the endotracheal tube of adult rats at expiration. Twenty-four hours after cardiopulmonary resuscitation, nerve cell injury and expression of tumor necrosis factor-α, interleukin-1β, and p38 mitogen activated protein kinase content were increased. Rats injected with Xuebijing, a Chinese herb compound preparation, exhibited normal cellular structure and morphology, dense neuronal cytoplasm, and decreased tumor necrosis factor-α, interleukin-1β, and p38 mitogen activated protein kinase expression at 24 hours following cardiopulmonary resuscitation. These data suggest that Xuebijing can attenuate neuronal injury induced by hypoxia and reperfusion during cardiopulmonary resuscitation.展开更多
Objective:The objective of this study was to investigate the clinical efficacy and safety of treating sepsis patients with Xuebijing injection(XBJI).Methods:We conducted a retrospective analysis of 418 patients who ex...Objective:The objective of this study was to investigate the clinical efficacy and safety of treating sepsis patients with Xuebijing injection(XBJI).Methods:We conducted a retrospective analysis of 418 patients who experienced severe infections and were treated with XBJI from June 2018 to June 2021.Propensity score matching was used to match the patient cases.The study population included 209 pairs of cases(418 individuals),and the analysis included data from before and after a 14-day course of treatment with carbapenem alone,or carbapenem with XBJI.Results:There were no significant differences in the 14-day mortality or length of hospital stay(P>0.05)between the two groups.The combined treatment group had more patients with C-reactive protein that returned to normal levels(compared to baseline)than the non-combined treatment group(14.4%vs 8.1%;odds ratio[OR]:0.528;95%confidence interval[CI]:0.282–0.991;P=0.026).Similarly,the combined treatment group had higher procalcitonin attainment rate(55.0%vs 39.7%;OR:0.513;95%CI:0.346–0.759;P=0.001)than the non-combined treatment group.Further,more patients in the combined treatment group achieved normal creatinine levels than in the non-combined treatment group(64.1%vs54.1%;OR:0.659;95%CI:0.445–0.975;P=0.037).Conclusion:The combination of XBJI with carbapenem did not reduce the 14-day mortality rate of patients with severe infection,but it was able to reduce the level of inflammatory factors in patients with sepsis,and had a protective effect on liver and kidney function.Please cite this article as:Gong ZT,Yang HX,Zhu BB,Liu HH,Siri GL.Clinical efficacy of Xuebijing injection for the treatment of sepsis:A retrospective cohort study.J Integr Med.2024;22(6):645–651.展开更多
OBJECTIVE:To evaluate the effectiveness and safety of Xuebijing injection(XBJ)on coronavirus disease 2019(COVID-19)in patients.METHODS:Related studies on multiple biological databases and websites were searched up to ...OBJECTIVE:To evaluate the effectiveness and safety of Xuebijing injection(XBJ)on coronavirus disease 2019(COVID-19)in patients.METHODS:Related studies on multiple biological databases and websites were searched up to December 11,2021 without language and publication time restrictions.Review Manager V.5.3 and Stata 14 software were used for data analysis.RESULTS:Seven studies were finally included.The Meta-analysis showed that compared with the routine treatment alone,XBJ combined with the routine treatment can reduce the 28-day mortality(OR=0.3,95%CI:0.12,0.74),C-reactive protein(MD=-12.8,95%CI:-23.13,-3.46),erythrocyte sedimentation rate(MD=-9.32,95%CI:-14.66,-3.98)and interleukin-6(SMD=-0.6,95%CI:-1.04,-0.17)levels and increase the leukocyte(MD=0.73,95%CI:0.42,1.04)and lymphocyte count(MD=0.18,95%CI:0.07,0.29)in peripheral blood;additionally,it has no obvious side effects(OR=1.11,95%CI:0.65,1.9).There was no evidence that the XBJ combined therapy can improve the nucleic acid conversion rate and computed tomography improvement rate of COVID-19 patients.CONCLUSIONS:Preliminary evidence suggests that XBJ combined with routine treatment seems to be more effective than routine treatment for patients with COVID-19.Limited by the number and quality of included papers,this finding still needs further validation by more studies.展开更多
Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:Th...Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:The TCMSP database was employed to collect and screen the active ingredients of the Chinese herb contained in the XBJ injection.The GeneCards database and STRING database were applied to collect and expand the targets of COVID-19 and compare and screen the related targets of COVID-19 by XBJ injection.Cytoscape was employed to build a network connecting Chinese medicine,compounds,targets,disease,and topology analysis was performed via the Network Analyzer to screen the key ingredients and targets.The software of Schrödinger molecular docking was used to verify the binding activity of the key ingredients of XBJ injection and the key targets of COVID-19.Metascape platform and DAVID database were utilized to conduct Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis on the key targets of COVID-19 treated by XBJ injection.Results:Eight key compounds and 15 key targets were screened and verified by molecular docking;these key compounds included luteolin,quercetin,baicalein,and kaempferol.The key targets included DPP4,AR,ESR1,CALM1,and protein kinase 1.Gene Ontology analysis involved an apoptosis and hypoxia reaction and the changes in blood vessel morphology.Kyoto Encyclopedia of Genes and Genomes analysis involved signaling pathways of hypoxia inducible factor-1,VEGF,and PI3K/AKT/NF-κB.Conclusion:The mechanism of XBJ injection when used to treat COVID-19 should be further investigated as the key compounds in XBJ regulated the expression of key targets such as protein kinase 1,VEGF-A,B-cell lymphoma-2,and TNF,which affected the COVID-19 receptors such as angiotensin-converting enzyme 2 and signaling pathways like hypoxia inducible factor-1,PI3K-Akt,and NF-κB,which alleviated the inflammation,respiratory distress,and hypoxia caused by COVID-19 infection.展开更多
Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensiv...Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensive care unit(ICU)were randomly divided into the control group and the treatment group with 35 cases in each group.Both groups were given routine treatment such as anti-inflammatory drugs,rehydration,expectorant,and nutritional support,while the treatment group was additionally given Xuebijing injection.Serum C-reactive protein(CRP),clinical pulmonary infection score(CPIS),acute physiology,and chronic health scoreⅡ(APACHEⅡ)were recorded before treatment,the 3rd and 7th day after treatment.The duration of antibiotic use,mechanical ventilation,ICU stay,and mortality during 28 days was recorded.Results:There was no significant difference in CRP,CPIS,and APACHEⅡbetween the two groups before treatment(P>0.05).The improvement of CRP,CPIS,and APACHEⅡin the treatment group was better than those in the control group on the 3 and 7 days after treatment,and the differences were statistically significant(P<0.05).The duration of antibiotic use,mechanical ventilation,and ICU stay in the treatment group were less than those in the control group(P<0.05).The 28-day mortality of the treatment group was lower than that of the control group,but the difference was not statistically significant(P>0.05).Conclusions:Xuebijing injection can improve the inflammatory indexes of patients with ventilator-associated pneumonia,and can partly improve the prognosis.展开更多
BACKGROUND:This study aims to explore whether Xuebijing(XBJ) can improve intestinal microcirculation dysfunction in sepsis and its mechanism.METHODS:A rat model of sepsis was established by cecal ligation and puncture...BACKGROUND:This study aims to explore whether Xuebijing(XBJ) can improve intestinal microcirculation dysfunction in sepsis and its mechanism.METHODS:A rat model of sepsis was established by cecal ligation and puncture(CLP).A total of 30 male SD rats were divided into four groups:sham group,CLP group,XBJ + axitinib group,and XBJ group.XBJ was intraperitoneally injected 2 h before CLP.Hemodynamic data(blood pressure and heart rate) were recorded.The intestinal microcirculation data of the rats were analyzed via microcirculation imaging.Enzyme-linked immunosorbent assay(ELISA) kits were used to detect the serum levels of interleukin-6(IL-6),C-reactive protein(CRP),and tumor necrosis factor-α(TNF-α) in the rats.Histological analysis and transmission electron microscopy were used to analyze the injury of small intestinal microvascular endothelial cells and small intestinal mucosa in rats.The expression of vascular endothelial growth factor A(VEGF-A),phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),and phosphorylated Akt(p-Akt) in the small intestine was analyzed via Western blotting.RESULTS:XBJ improved intestinal microcirculation dysfunction in septic rats,alleviated the injury of small intestinal microvascular endothelial cells and small intestinal mucosa,and reduced the systemic inflammatory response.Moreover,XBJ upregulated the expression of VEGF-A,p-PI3K/total PI3K,and p-Akt/total Akt in the rat small intestine.CONCLUSION:XBJ may improve intestinal microcirculation dysfunction in septic rats possibly through the VEGF-A/PI3K/Akt signaling pathway.展开更多
Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Met...Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Methods:Anti-CD19 CAR-T cells were established based on FMC63 antibodies.Different doses of XBJ(1 and 10 mg/mL)were added to the culture system.Untreated anti-CD19 CAR-T cells served as negative controls.After 48-h co-culture,the effects of XBJ on CAR-T cell function were assessed.Carboxyfluorescein diacetate succinimidyl ester staining was used to assess the effect of XBJ on CAR-T cell proliferation.Flow cytometry,luciferase reporter gene assays,and real time cellular analysis were employed to evaluate the effects of XBJ on CAR-T cell cytotoxicity in vitro.RNA-sequencing was performed to analyze the effects of XBJ on CAR-T cell gene expression.Network pharmacology predicted potential XBJ therapeutic targets for CRS,which were verified in a THP-1 macrophage inflammation model.Results:XBJ enhanced both the proliferation and tumor killing capacities of CAR-T cells.Transcriptome analysis showed that XBJ treatment affects multiple genes and pathways in CAR-T cells,with differential gene enrichment in multiple cell proliferation and growth factor pathways.Potential targets for CRS control by XBJ were predicted using network pharmacology,and the inhibitory effect of XBJ on the expression of relevant genes was verified using a macrophage model.Conclusion:The results of this study indicate that XBJ can enhance the killing effect of CAR-T cells on tumor cells and that the mechanism is related to the regulation of T cell proliferation and activation.Moreover,XBJ inhibited excessive inflammation associated with CAR-T therapy.However,the current findings remain to be further validated through in vivo experiments.展开更多
基金supported by the National Natural Science Foundation of China(No.81571373,No.81601217 and No.82001491)Natural Science Foundation of Hubei Province of China(No.2017CFB627)+1 种基金Health Commission of Hubei Province Scientific Research Project(No.WJ2021M247)Scientific Research Fund of Wuhan Union Hospital(No.2019)。
文摘Objective:Xuebijing injection has been recommended as a therapeutic approach for individuals with severe and critical COVID-19.This study aims to explore the correlation of neutrophil to lymphocyte platelet ratio(NLPR)with the severity and prognosis of COVID-19,and the effect of XBJ on the prognosis of patients with COVID-19 in different inflammatory states.Methods:This was a retrospective study conducted at Wuhan Union Hospital in China.COVID-19patients admitted between November 1,2022 and February 1,2023 were included.In predicting prognosis for individuals with COVID-19,new inflammatory indicators were used,and their prognostic value was assessed by using Cox regression models and receiver operating characteristic curves.Furthermore,a calculation was made to determine the cutoff value for NLPR.Relative risk and Cox regression models were used to examine the effects of Xuebijing injection on prognosis in patient cohorts that had been stratified by the NLPR cutoff.Results:This research included 455 participants with COVID-19,with a mean age of 72 years.Several inflammatory indicators were found to be strongly correlated with prognosis,and NLPR shows the greatest predictive power.Patients with NLPR>3.29 exhibited a mortality rate of 17.3%,which was 6.2 times higher than in patients with NLPR≤3.29.Importantly,providing Xuebijing injection to patients with NLPR>3.29 was associated with a lower risk of 60-day all-cause mortality.However,there was no discernible improvement in survival among patients with NLPR≤3.29 who received Xuebijing injection.Conclusion:NLPR is the most reliable inflammatory marker for predicting prognosis among individuals with COVID-19,and can accurately identify individuals who may benefit from Xuebijing injection.
基金Supported by the National Natural Science Foundation of China (Grant No. 81101406,81071529)the Project of Medical Research of PLA BWS12J108
文摘OBJECTIVE： To evaluate the effects of Xuebijing （XBJ） injection in heat stroke （HS） rats and to inves- tigate the mechanisms underlying these effects. METHODS： Sixty anesthetized rats were random- ized into three groups and intravenously injected twice daily for 3 days with 4 mL XBJ （XBJ group） or phosphate buffered saline （HS and Sham groups） per kg body weight. HS was initiated in the HS and XBJ groups by placing rats in a simulated climate chamber （ambient temperature 40℃：, humidity 60% ）. Rectal temperature, aterial pressure, and heart rate were monitored and recorded. Time to HS onset and survival were determined, and serum concentrations of tumor necrosis factor （TNF）-α, in-terleukin （IL）-1β, IL-6, alanine-aminotransferase （ALT）, and aspartate-aminotransferase （AST） were measured. Hepatic tissue was harvested for patho- logical examination and electron microscopic ex- amination. Kupffer cells （KCs） were separated from liver at HS initiation, and the concentrations of se- creted TNF-α, IL-1β3 and IL-6 were measured. RESULTS： Time to HS onset and survival were signif- icantly longer in the XBJ than in the HS group. Moreover, the concentrations of TNF-α, IL-1β, IL-6, ALT and AST were lower and liver injury was milder in the XBJ than in the HS group. Heat-stress in- duced structural changes in KCs and hepatic cells were more severe in the HS than in the XBJ group and the concentrations of TNF-α, IL-1β and IL-6 se- creted by KCs were lower in the XBJ than in the HS group. CONCLUSION： XBJ can alleviate HS-induced sys- temic inflammatory response syndrome and liver injury in rats, and improve outcomes. These protec- tive effects may be due to the ability of XBJ to inhib- it cytokine secretion by KCs.
文摘Objective: To observe the effects of Xuebijing Injection (血必净注射液) in patients with severe pneumonia, and to explore the mechanism. Methods: Eighty cases of severe pneumonia are randomly assigned to the Xuebijing treatment (forty cases) and the control group (forty cases), with the same routine therapy provided in both groups. Clinical effective rates, inflammatory factors and organ function were observed in both groups. Results: The effective rate was higher in Xuebijing group than that of the control group (80.0% vs. 67.5%, P<0.05). As compared with the control group, the LDH, α1-AG, α1-AT levels and the peak body temperature decreased markedly with the Xuebijing treatment going, and the secretion of TNF-α, IL-6, IL-8 was suppressed in Xuebijing group; but no significant difference was found in leptin level. Conclusion: Xuebijing Injection may show a protective effect in patients with severe pneumonia. The mechanism is possibly with the decreased secretion of TNF-α, IL-6, and IL-8.
基金supported by the Double-Class University project(No.CPU2018GY19)the National Natural Science Foundation of China(No.81874331)。
文摘As a representative drug for the treatment of severe community-acquired pneumonia and sepsis,Xuebijing(XBJ)injection is also one of the recommended drugs for the prevention and treatment of coronavirus disease 2019(COVID-19),but its treatment mechanism for COVID-19 is still unclear.Therefore,this study aims to explore the potential mechanism of XBJ injection in the treatment of COVID-19 employing network pharmacology and molecular docking methods.The corresponding target genes of 45 main active ingredients in XBJ injection and COVID-19 were obtained by using multiple database retrieval and literature mining.102 overlapping targets of them were screened as the core targets for analysis.Then built the PPI network,TCM-compound-target-disease,and disease-target-pathway networks with the help of Cytoscape 3.6.1 software.After that,utilized DAVID to perform gene ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to predict the action mechanism of overlapping targets.Finally,by applying molecular docking technology,all compounds were docked with COVID-193 CL protease(3 CLpro),spike protein(S protein),and angiotensin-converting enzyme II(ACE2).The results indicated that quercetin,luteolin,apigenin and other compounds in XBJ injection could affect TNF,MAPK1,IL6 and other overlapping targets.Meanwhile,anhydrosafflor yellow B(AHSYB),salvianolic acid B(SAB),and rutin could combine with COVID-19 crucial proteins,and then played the role of anti-inflammatory,antiviral and immune response to treat COVID-19.This study revealed the multiple active components,multiple targets,and multiple pathways of XBJ injection in the treatment of COVID-19,which provided a new perspective for the study of the mechanism of traditional Chinese medicine(TCM)in the treatment of COVID-19.
基金supported by the National Natural Science Foundation of China(Nos.82130001,82200089,8173000,81800077,and 82070045)Shanghai Sailing Program(No.22YF1406100)+3 种基金the National Key R&D Plan(No.2020YFC2003700)the Science and Technology Commission of Shanghai Municipality(Nos.20Z11901000,20DZ2261200,and 20XD1401200)Shanghai Municipal Science and Technology Major Project,Clinical Research Plan of SHDC(No.SHDC2020CR5010-002)Shanghai Municipal Key Clinical Specialty(No.shslczdzk02201).
文摘Acute lung injury/acute respiratory distress syndrome(ALI/ARDS)is characterized by diffuse alveolar injury primarily caused by an excessive inflammatory response.Regrettably,the lack of effective pharmacotherapy currently available contributes to the high mortality rate in patients with this condition.Xuebijing(XBJ),a traditional Chinese medicine recognized for its potent anti-inflammatory properties,exhibits promise as a potential therapeutic agent for ALI/ARDS.This study aimed to explore the preventive effects of XBJ on ALI and its underlying mechanism.To this end,we established an LPS-induced ALI model and treated ALI mice with XBJ.Our results demonstrated that pre-treatment with XBJ significantly alleviated lung inflammation and increased the survival rate of ALI mice by 37.5%.Moreover,XBJ substantially suppressed the production of TNF-α,IL-6,and IL-1βin the lung tissue.Subsequently,we performed a network pharmacology analysis and identified identified 109 potential target genes of XBJ that were mainly involved in multiple signaling pathways related to programmed cell death and anti-inflammatory responses.Furthermore,we found that XBJ exerted its inhibitory effect on gasdermin-E-mediated pyroptosis of lung cells by suppressing TNF-αproduction.Therefore,this study not only establishes the preventive efficacy of XBJ in ALI but also reveals its role in protecting alveolar epithelial cells against gasdermin-E-mediated pyroptosis by reducing TNF-αrelease.
基金This work was supported by the second batch of"Ten thousand plan"-National high-level talents special support plan(No.W02020052)National Natural Science Foundation of China(No.82072247,82204937)+1 种基金Natural Science Foundation of Beijing(No.L222150)the China Scholarship Council(No.202206557005)。
文摘Severe pneumonia is one of the most common infectious diseases and the leading cause of sepsis and septic shock.Preventing infection,balancing the patient’s immune status,and anti-coagulation therapy are all important elements in the treatment of severe pneumonia.As multi-target agents,Xuebijing injection(XBJ)has shown unique advantages in targeting complex conditions and saving the lives of patients with severe pneumonia.This review outlines progress in the understanding of XBJ’s anti-inflammatory,endotoxin antagonism,and anticoagulation effects.From the hundreds of publications released over the past few years,the key results from representative clinical studies of XBJ in the treatment of severe pneumonia were selected and summarized.XBJ was observed to effectively suppress the release of pro-inflammatory cytokines,counter the effects of endotoxin,and assert an anticoagulation effect in most clinical trials,which are consistent with experimental studies.Collectively,this evidence suggests that XBJ could play an important and expanding role in clinical medicine,especially for sepsis,septic shock and severe pneumonia.
文摘OBJECTIVE： Paraquat （PQ） poisoning-induced pul- monary fibrosis causes asphyxiation and death. The therapeutic potential of intravenous Xuebijing ther- apy in PQ poisoning patients and its underlying im- munomodulatory effects on transforming growth factor （TGF）-131 and procollagen type III peptide （PIIIP） were investigated. METHODS： Thirty-six acute PQ poisoning patients were randomly assigned to conventional therapy （Group A） and intravenous Xuebijing administra- tion plus conventional therapy （Group 13）. Twenty volunteers served as controls （Group C）. Blood sam- ples were collected upon admission （day O） and at post-treatment days 5, 10, and 14. TGF-β1 and PIIIP concentrations were determined by ELISA and ana- lyzed for intra- and inter-group differences over time. One-month follow-up was conducted for de- termining the mortality rate. RESULTS： TGF-β1 and PIIIP levels were significantly higher in PQ poisoning patients and increased over time （Groups A and B vs C, P〈0.01）. However, the TGF-β1 and PIIIP levels were consistently significant- ly lower in Group B compared with those of Group A （P〈0.01）. The 1-month mortality rate was also lower in Group B compared with that of Group A （P〈0.05）. PQ poisoning patients showed remark- ably high levels of TGF-β1 and PIIIP, which increased as PQ-induced pulmonary fibrosis progressed. CONCLUSION： Treatment with intravenous Xuebi- jing plus conventional therapy significantly low- ered TGF-β1 and PIIIP levels, which indicates thera- peutic efficacy in the treatment of PQ poisoning pa- tients.
基金Supported by Key Medical Research Subject of Hebei Province(the Protective Effect and Mechanism of Xuebijing Injection on Cardiac Function in Patients With Sepsis,No.ZD20140379)
文摘OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myocardial injury were recruited, and were randomly divided into two groups: treatment group and control group. All patients in two groups received conventional cluster treatment, the patients in treatment group additional received Xuebijing injection dissolved in0.9% sodium chloride injection, and the patients in control group received the same amount of 0.9%sodium chloride injection. At the beginning of treatment and 3, 7 and 10-day after treatment, lab-oratory indicators of cardiac troponin Ⅰ(cTnI),N-terminal pro B-type natriuretic peptide(NT-pro BNP) and procalcitonin(PCT) were respectively tested in venous blood. The patient's length of stay in Intensive Care Unit(ICU) and the mortality in 28 days were recorded.RESULTS: At 3, 7 and 10-day after treatment, the improvements of c Tn I, NT-pro BNP and PCT in treatment group were better than those in control group, and the differences were statistically significant(P < 0.05). The mortality of treatment group in28 days was not significantly different from that of control group(P > 0.05). The ICU length of stay of treatment group was shorter than that of control group(P > 0.05).CONCLUSION: Xuebijing injection could improve the levels of c Tn I, NT-pro BNP and PCT in patients with septic myocardial injury.and it had a protective effect on myocardial injury.
基金funded by grants from the National Natural Science Foundation of China(Grant Nos.:82074176 and 81503345)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(Grant No.:ZYYCXTD-C-202009)the National Key R&D Program of China(Grant No.:2018YFC1704500).
文摘XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based assay for quality evaluation of XueBiJing.Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel.Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition,pharmacokinetics,and pharmacodynamics.A selectivity test(for“similarity of response”)was developed to identify and minimize interference by nontarget constituents.Then,an intercept test was developed to fulfill“linearity through zero”for each analyte(absolute ratio of intercept to C response,<2%).Using the newly developed assays,we analyzed samples from 33 batches of XueBiJing,manufactured over three years,and found small batch-to-batch variability in contents of the marker constituents(4.1%-14.8%),except for senkyunolide I(26.5%).
基金supported by Hunan Province Key Research and Development Program(2020SKC2004).
文摘BACKGROUND:Xuebijing(XBJ)can alleviate the inflammatory response,improve organ function,and shorten the intensive care unit(ICU)stay in patients with pyogenic liver abscess(PLA)complicated with sepsis,but the molecular mechanisms have not been elucidated.This study aimed to explore the molecular mechanism of XBJ in treating PLA complicated with sepsis using a network pharmacology approach.METHODS:The active ingredients and targets of XBJ were retrieved from the ETCM database.Potential targets related to PLA and sepsis were retrieved from the GeneCards,PharmGKB,DisGeNet,Online Mendelian Inheritance in Man(OMIM),Therapeutic Targets Database(TTD),and DrugBank databases.The targets of PLA complicated with sepsis were mapped to the targets of XBJ to identify potential treatment targets.Protein-protein interaction networks were analyzed using the STRING database.Potential treatment targets were imported into the Metascape platform for Gene Ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.Molecular docking was performed to validate the interactions between active ingredients and core targets.RESULTS:XBJ was found to have 54 potential treatment targets for PLA complicated with sepsis.Interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor(TNF)were identifi ed as core targets.KEGG enrichment analysis revealed important pathways,including the interleukin-17(IL-17)signaling pathway,the TNF signaling pathway,the nuclear factor-kappa B(NF-κB)signaling pathway,and the Toll-like receptor(TLR)signaling pathway.Molecular docking experiments indicated stable binding between XBJ active ingredients and core targets.CONCLUSION:XBJ may exert therapeutic eff ects on PLA complicated with sepsis by modulating signaling pathways,such as the IL-17,TNF,NF-κB,and TLR pathways,and targeting IL-1β,IL-6,and TNF.
文摘BACKGROUND Cardiopulmonary bypass(CPB)is an essential procedure for maintaining the blood supply to vital organs in patients undergoing cardiac surgery.However,perioperative cardiac injury related to CPB remains a severe complication in these patients.Cardiac protection is important for patients undergoing CPB.AIM To evaluate the potential cardioprotective efficacy of the Chinese medicine preparation Xuebijing injection(XBJ)in patients undergoing CPB.METHODS Sixty patients undergoing cardiac surgery with CPB were randomly allocated to the XBJ and control groups(saline).XBJ was administered intravenously three times:12 h prior to surgery,at the beginning of the surgery,and 12 h after the second injection.Cardiac function was evaluated by echocardiography 48 h after surgery.Circulating inflammation-and oxidative-stress-related markers were measured.Clinical outcomes related to intensive care unit(ICU)stay were recorded.RESULTS Compared to control treatment,XBJ was associated with improved PaO2/FiO2 and cardiac systolic function,but reduced troponin I and creatine kinase fraction after surgery(all P<0.05).The circulating concentrations of tumor necrosis factor-α,interleukin(IL)-1βand IL-8 in the XBJ group were significantly lower than those in the control group(all P<0.05),whereas the circulating concentration of IL-10 was significantly higher in the XBJ group(P<0.05).In addition,the lengths of ICU stay and hospitalization after surgery tended to be shorter in the XBJ group than in the control group,although the differences were not significant.CONCLUSION Perioperative administration of XBJ was associated with attenuated cardiac injury during CPB,likely via anti-inflammatory and antioxidative mechanisms.
文摘BACKGROUND: The study aimed to investigate the therapeutic benefits of intravenous Xuebijing on acute kidney injury(AKI) in rats with paraquat intoxication.METHODS: Male Sprague-Dawley rats were randomly divided equally into three groups: sham group(n=8), paraquat group(n=8) and Xuebijing-treated group(n=8) using a random number table. The rats were intraperitoneally injected with 50 mg/kg of paraquat. One hour after paraquat administration, the rats were treated intravenously with Xuebijing(8 mL /kg). At 12 hours after paraquat administration, serum was collected to evaluate kidney function, then the rats were sacrificed and kidney samples were immediately harvested. AKI scores were evaluated by renal histopathology and pro-in? ammatory cytokines mR NA levels in kidney were assayed using real-time RT-PCR.RESULTS: Serum urea nitrogen, creatinine and AKI scores were significantly higher in the paraquat group, compared with the sham group(P<0.05, respectively). Moreover, interleukin(IL)-1β, IL-6 and TNF-α m RNA levels were signi? cantly higher in the paraquat group(P<0.01, respectively). However, intravenous Xuebijing signi? cantly decreased serum urea nitrogen, creatinine, AKI scores and IL-1β, IL-6 and TNF-α m RNA levels, compared with the paraquat group(P<0.05, respectively).CONCLUSION: Intravenous Xuebijing attenuates AKI following paraquat poisoning by suppressing in? ammatory response.
基金CAMS Medicine and Health Technology Innovation ProjectGrant/Award Number:2021-I2M-1-060 and 2021-RC310-010+1 种基金National Natural Science Foundation of ChinaGrant/Award Number:81972975。
文摘Background:Gastrointestinal(GI)injury is one of the most common side effects of radiotherapy.However,there is no ideal therapy method except for symptomatic treatment in the clinic.Xuebijing(XBJ)is a traditional Chinese medicine,used to treat sepsis by injection.In this study,the protective effects of XBJ on radiation-i nduced intestinal injury(RⅢ)and its mechanism were explored.Methods:The effect of XBJ on survival of irradiated C57BL/6 mice was monitored.Histological changes including the number of crypts and the length of villi were evaluated by H&E.The expression of Lgr5^(+)intestinal stem cells(ISCs),Ki67^(+)cells,villin and lysozymes were examined by immunohistochemistry.The expression of cytokines in the intestinal crypt was detected by RT-PCR.DNA damage and apoptosis rates in the small intestine were also evaluated by immunofluorescence.Results:In the present study,XBJ improved the survival rate of the mice after 8.0and 9.0 Gy total body irradiation(TBI).XBJ attenuated structural damage of the small intestine,maintained regenerative ability and promoted proliferation and differentiation of crypt cells,decreased apoptosis rate and reduced DNA damage in the intestine.Elevation of IL-6 and TNF-α was limited,but IL-1,TNF-β and IL-10 levels were increased in XBJ-treated group after irradiation.The expression of Bax and p53 were decreased after XBJ treatment.Conclusions:Taken together,XBJ provides a protective effect on RⅢby inhibiting inflammation and blocking p53-related apoptosis pathway.
基金a grant from the Science and Technology Department of Jilin Province,No. 200705172
文摘We established a rat model of cardiac arrest by clamping the endotracheal tube of adult rats at expiration. Twenty-four hours after cardiopulmonary resuscitation, nerve cell injury and expression of tumor necrosis factor-α, interleukin-1β, and p38 mitogen activated protein kinase content were increased. Rats injected with Xuebijing, a Chinese herb compound preparation, exhibited normal cellular structure and morphology, dense neuronal cytoplasm, and decreased tumor necrosis factor-α, interleukin-1β, and p38 mitogen activated protein kinase expression at 24 hours following cardiopulmonary resuscitation. These data suggest that Xuebijing can attenuate neuronal injury induced by hypoxia and reperfusion during cardiopulmonary resuscitation.
基金financially supported by Science and Technology Program of the Joint Fund of Scientific Research for the Public Hospitals of Inner Mongolia Academy of Medical Sciences(No.2023GLLH0047)Inner Mongolia Medical University Science and Technology million project joint fund(No.YKD2023LH076)。
文摘Objective:The objective of this study was to investigate the clinical efficacy and safety of treating sepsis patients with Xuebijing injection(XBJI).Methods:We conducted a retrospective analysis of 418 patients who experienced severe infections and were treated with XBJI from June 2018 to June 2021.Propensity score matching was used to match the patient cases.The study population included 209 pairs of cases(418 individuals),and the analysis included data from before and after a 14-day course of treatment with carbapenem alone,or carbapenem with XBJI.Results:There were no significant differences in the 14-day mortality or length of hospital stay(P>0.05)between the two groups.The combined treatment group had more patients with C-reactive protein that returned to normal levels(compared to baseline)than the non-combined treatment group(14.4%vs 8.1%;odds ratio[OR]:0.528;95%confidence interval[CI]:0.282–0.991;P=0.026).Similarly,the combined treatment group had higher procalcitonin attainment rate(55.0%vs 39.7%;OR:0.513;95%CI:0.346–0.759;P=0.001)than the non-combined treatment group.Further,more patients in the combined treatment group achieved normal creatinine levels than in the non-combined treatment group(64.1%vs54.1%;OR:0.659;95%CI:0.445–0.975;P=0.037).Conclusion:The combination of XBJI with carbapenem did not reduce the 14-day mortality rate of patients with severe infection,but it was able to reduce the level of inflammatory factors in patients with sepsis,and had a protective effect on liver and kidney function.Please cite this article as:Gong ZT,Yang HX,Zhu BB,Liu HH,Siri GL.Clinical efficacy of Xuebijing injection for the treatment of sepsis:A retrospective cohort study.J Integr Med.2024;22(6):645–651.
基金National Natural Science Foundation of China:Effects of"Qingmang Yihao recipe"on retinal Ganglion Cells after Experimental Optic Nerve Injury(No.81874491)Exploring the Protective Mechanism of AstragalosideⅣon Retinal Ganglion Cells after Trauma based on Autophagy Mediated by AMPK-mTORULK1/2 signaling pathway(No.81973909)。
文摘OBJECTIVE:To evaluate the effectiveness and safety of Xuebijing injection(XBJ)on coronavirus disease 2019(COVID-19)in patients.METHODS:Related studies on multiple biological databases and websites were searched up to December 11,2021 without language and publication time restrictions.Review Manager V.5.3 and Stata 14 software were used for data analysis.RESULTS:Seven studies were finally included.The Meta-analysis showed that compared with the routine treatment alone,XBJ combined with the routine treatment can reduce the 28-day mortality(OR=0.3,95%CI:0.12,0.74),C-reactive protein(MD=-12.8,95%CI:-23.13,-3.46),erythrocyte sedimentation rate(MD=-9.32,95%CI:-14.66,-3.98)and interleukin-6(SMD=-0.6,95%CI:-1.04,-0.17)levels and increase the leukocyte(MD=0.73,95%CI:0.42,1.04)and lymphocyte count(MD=0.18,95%CI:0.07,0.29)in peripheral blood;additionally,it has no obvious side effects(OR=1.11,95%CI:0.65,1.9).There was no evidence that the XBJ combined therapy can improve the nucleic acid conversion rate and computed tomography improvement rate of COVID-19 patients.CONCLUSIONS:Preliminary evidence suggests that XBJ combined with routine treatment seems to be more effective than routine treatment for patients with COVID-19.Limited by the number and quality of included papers,this finding still needs further validation by more studies.
基金This study was supported by the Foundation of Health Commission of Hebei Province(20190123)the Natural Science Foundation of Hebei Province of China(H2018201179).
文摘Background:To evaluate the mechanism of Chinese patent drug Xuebijing(XBJ)injection in the treatment of a new coronavirus disease 2019(COVID-19)based on network pharmacology and molecular docking technology.Methods:The TCMSP database was employed to collect and screen the active ingredients of the Chinese herb contained in the XBJ injection.The GeneCards database and STRING database were applied to collect and expand the targets of COVID-19 and compare and screen the related targets of COVID-19 by XBJ injection.Cytoscape was employed to build a network connecting Chinese medicine,compounds,targets,disease,and topology analysis was performed via the Network Analyzer to screen the key ingredients and targets.The software of Schrödinger molecular docking was used to verify the binding activity of the key ingredients of XBJ injection and the key targets of COVID-19.Metascape platform and DAVID database were utilized to conduct Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis on the key targets of COVID-19 treated by XBJ injection.Results:Eight key compounds and 15 key targets were screened and verified by molecular docking;these key compounds included luteolin,quercetin,baicalein,and kaempferol.The key targets included DPP4,AR,ESR1,CALM1,and protein kinase 1.Gene Ontology analysis involved an apoptosis and hypoxia reaction and the changes in blood vessel morphology.Kyoto Encyclopedia of Genes and Genomes analysis involved signaling pathways of hypoxia inducible factor-1,VEGF,and PI3K/AKT/NF-κB.Conclusion:The mechanism of XBJ injection when used to treat COVID-19 should be further investigated as the key compounds in XBJ regulated the expression of key targets such as protein kinase 1,VEGF-A,B-cell lymphoma-2,and TNF,which affected the COVID-19 receptors such as angiotensin-converting enzyme 2 and signaling pathways like hypoxia inducible factor-1,PI3K-Akt,and NF-κB,which alleviated the inflammation,respiratory distress,and hypoxia caused by COVID-19 infection.
基金This work was supported by the Talent Funding Project of Tangshan City in Hebei Province(No.a201902005).
文摘Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensive care unit(ICU)were randomly divided into the control group and the treatment group with 35 cases in each group.Both groups were given routine treatment such as anti-inflammatory drugs,rehydration,expectorant,and nutritional support,while the treatment group was additionally given Xuebijing injection.Serum C-reactive protein(CRP),clinical pulmonary infection score(CPIS),acute physiology,and chronic health scoreⅡ(APACHEⅡ)were recorded before treatment,the 3rd and 7th day after treatment.The duration of antibiotic use,mechanical ventilation,ICU stay,and mortality during 28 days was recorded.Results:There was no significant difference in CRP,CPIS,and APACHEⅡbetween the two groups before treatment(P>0.05).The improvement of CRP,CPIS,and APACHEⅡin the treatment group was better than those in the control group on the 3 and 7 days after treatment,and the differences were statistically significant(P<0.05).The duration of antibiotic use,mechanical ventilation,and ICU stay in the treatment group were less than those in the control group(P<0.05).The 28-day mortality of the treatment group was lower than that of the control group,but the difference was not statistically significant(P>0.05).Conclusions:Xuebijing injection can improve the inflammatory indexes of patients with ventilator-associated pneumonia,and can partly improve the prognosis.
基金supported by a grant from National Natural Science Foundation of China (82272196)。
文摘BACKGROUND:This study aims to explore whether Xuebijing(XBJ) can improve intestinal microcirculation dysfunction in sepsis and its mechanism.METHODS:A rat model of sepsis was established by cecal ligation and puncture(CLP).A total of 30 male SD rats were divided into four groups:sham group,CLP group,XBJ + axitinib group,and XBJ group.XBJ was intraperitoneally injected 2 h before CLP.Hemodynamic data(blood pressure and heart rate) were recorded.The intestinal microcirculation data of the rats were analyzed via microcirculation imaging.Enzyme-linked immunosorbent assay(ELISA) kits were used to detect the serum levels of interleukin-6(IL-6),C-reactive protein(CRP),and tumor necrosis factor-α(TNF-α) in the rats.Histological analysis and transmission electron microscopy were used to analyze the injury of small intestinal microvascular endothelial cells and small intestinal mucosa in rats.The expression of vascular endothelial growth factor A(VEGF-A),phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),and phosphorylated Akt(p-Akt) in the small intestine was analyzed via Western blotting.RESULTS:XBJ improved intestinal microcirculation dysfunction in septic rats,alleviated the injury of small intestinal microvascular endothelial cells and small intestinal mucosa,and reduced the systemic inflammatory response.Moreover,XBJ upregulated the expression of VEGF-A,p-PI3K/total PI3K,and p-Akt/total Akt in the rat small intestine.CONCLUSION:XBJ may improve intestinal microcirculation dysfunction in septic rats possibly through the VEGF-A/PI3K/Akt signaling pathway.
基金supported by the Beijing Double Class One Human Resource Office-High-Level-Jianxun Wang's Research Team Funding Project of China(1000041510155)the Research Startup Fund Program at the Beijing University of Chinese Medicine(90011451310032).
文摘Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Methods:Anti-CD19 CAR-T cells were established based on FMC63 antibodies.Different doses of XBJ(1 and 10 mg/mL)were added to the culture system.Untreated anti-CD19 CAR-T cells served as negative controls.After 48-h co-culture,the effects of XBJ on CAR-T cell function were assessed.Carboxyfluorescein diacetate succinimidyl ester staining was used to assess the effect of XBJ on CAR-T cell proliferation.Flow cytometry,luciferase reporter gene assays,and real time cellular analysis were employed to evaluate the effects of XBJ on CAR-T cell cytotoxicity in vitro.RNA-sequencing was performed to analyze the effects of XBJ on CAR-T cell gene expression.Network pharmacology predicted potential XBJ therapeutic targets for CRS,which were verified in a THP-1 macrophage inflammation model.Results:XBJ enhanced both the proliferation and tumor killing capacities of CAR-T cells.Transcriptome analysis showed that XBJ treatment affects multiple genes and pathways in CAR-T cells,with differential gene enrichment in multiple cell proliferation and growth factor pathways.Potential targets for CRS control by XBJ were predicted using network pharmacology,and the inhibitory effect of XBJ on the expression of relevant genes was verified using a macrophage model.Conclusion:The results of this study indicate that XBJ can enhance the killing effect of CAR-T cells on tumor cells and that the mechanism is related to the regulation of T cell proliferation and activation.Moreover,XBJ inhibited excessive inflammation associated with CAR-T therapy.However,the current findings remain to be further validated through in vivo experiments.
