Objective:This study aimed to observe the effects of xiusanzhen acupuncture(olfactory three-needling therapy)on the behavior,hippocampal histopathology and microglial(MG)activation of rats with vascular dementia(VD),a...Objective:This study aimed to observe the effects of xiusanzhen acupuncture(olfactory three-needling therapy)on the behavior,hippocampal histopathology and microglial(MG)activation of rats with vascular dementia(VD),and to assess the role of the trigeminal nerve.Methods:Sprague-Dawley(SD)rats were randomly assigned to the sham-operation,model,xiusanzhen,and trigeminal neurotomy groups(n=15 per group).Bilateral common carotid artery ligation was performed to prepare the VD models.Models of trigeminal neurotomy were prepared through the excision of the frontal nerve and infraorbital nerve.Xiusanzhen acupuncture was delivered via electric stimulation.The acupoints selected were bilateral“Yingxiang(LI20)”and“Yintang(EX-HN3)”,and the stimulation parameters were as follows:disperse-dense wave,at a frequency of 2/15HZ and intensity of 1 mA.The course of treatment was once daily,with one course lasting five days,followed by an interval of two days,yielding a total of four courses.Behavioral changes were detected using the Morris water maze,changes in histomorphology in the hippocampal CA1 region were determined with hematoxylin and eosin(HE)staining,and MG activation in the hippocampal CA1 region was detected using immunofluorescence.Results:The escape latency:From days 3 to 5,the escape latency was higher in the model group compared to the sham-operation group(P<0.05),but was lower in the xiusanzhen group compared to the model and trigeminal neurotomy groups(P<0.05).The frequency for platform crossing and swimming distance:both were reduced in the model group compared to the sham-operation group(P<0.01);and were elevated in the xiusanzhen group compared to the model group and trigeminal neurotomy group(P<0.01).Hippocampal pathomorphological changes:In the sham-operation group,the morphological structure and nucleoli were well-defined;in the model group and the trigeminal neurotomy group,cell numbers were reduced and karyopyknosis increased;while in the xiusanzhen group,the cell numbers were elevated and karyopyknosis was reduced compared with the model group.MG:the positive rate was higher in the model group compared to the sham-operation group(P<0.05);lower in the xiusanzhen group compared to the model group(P<0.05);and higher in the trigeminal neurotomy group compared to the xiusanzhen group(P<0.05).Conclusion:Xiusanzhen acupuncture attenuates cognitive impairment in VD rats,inhibits MG activation,and reduces hippocampal tissue damage.Its effects depend on the structural integrity of the trigeminal nerve.展开更多
Objective To observe the effects of Xiusanzhen treatment on the ultrastructure of olfactory bulb and the expression of substantia nigra glial fibrillary acidic protein(GFAP)in mice with Parkinson's disease(PD)indu...Objective To observe the effects of Xiusanzhen treatment on the ultrastructure of olfactory bulb and the expression of substantia nigra glial fibrillary acidic protein(GFAP)in mice with Parkinson's disease(PD)induced by lipopolysaccharide(LPS),and to provide methods and evidence for early prevention and treatment of PD.Methods Forty C57BL/6 male mice were randomly divided into a blank group,a model group,an electroacupuncture(EA)group and a medication group,10 mice in each one.The mice in the model group,EA group and medication group were treated with 30-day nasal perfusion of LPS to establish PD model.From the first day of model establishment,the mice in the EA group were treated with electroacupuncture at bilateral“Yingxiang”(LI 20)and“Yintang”(GV 29)for 20 min,once a day;5-day treatment was taken as one session,and 4 sessions were given with an interval of 2 days between sessions.The mice in the medication group were treated with intraperitoneal injection of L-DOPA,10 mg/mL,once a day;5-day treatment was taken as one session,and 4 sessions were given with an interval of 2 days between sessions.After treatment,the behavioristics changes were observed by using footprint analysis and swimming test score;the ultrastructure of olfactory bulb was observed by using transmission electron microscopy;the expression of GFAP in substantia nigra was measured by using western blot method.Results ① After model establishment,the mice in the model group,the EA group and medication group showed significant symptoms of quiver and fear of chill,and the BMI was significantly lower than that in the blank group(all P<0.05).The results of behavioristics test indicated footprint length and swimming time in the model group,the EA group and medication group was significantly lower than those in the blank group(all P<0.01),indicating the successful establishment of PD model.② After onesession treatment,the symptoms of quiver and fear of chill were not observed in the EA group.After 4-session treatment,the BMI in the EA group was significantly higher than that in the model group(P<0.05);the symptoms of quiver and fear of chill were not observed in the medication group,but the BMI was similar with the model group(P>0.05).③ After treatment,the footprint and swimming time in the model group were significantly lower than that in the blank group(both P<0.01);the footprint and swimming time in the EA group and medication group were significantly higher than those in the model group(all P<0.01).④ After treatment,compared with the blank group,the organelles and ultrastructure of olfactory bulb in the model group were significantly improved;the ultrastructure of olfactory bulb in the EA group was improved compared with that in the model group.⑤ After treatment,the expression of substantia nigra GFAP in the model group was significantly higher than that in the blank group(P<0.01);the expression of substantia nigra GFAP in the EA group and medication group was significantly lower than that in the model group(both P<0.05).Conclusion The early treatment of Xiusanzhen can improve behavioral disorders in LPS-induced early PD mice,and the mechanism may be related to the regulation of olfactory disorders and the expression of GFAP in substantia nigra.展开更多
基金Supported by The National Natural Science Foundation of China Young Scientists Fund:82004480。
文摘Objective:This study aimed to observe the effects of xiusanzhen acupuncture(olfactory three-needling therapy)on the behavior,hippocampal histopathology and microglial(MG)activation of rats with vascular dementia(VD),and to assess the role of the trigeminal nerve.Methods:Sprague-Dawley(SD)rats were randomly assigned to the sham-operation,model,xiusanzhen,and trigeminal neurotomy groups(n=15 per group).Bilateral common carotid artery ligation was performed to prepare the VD models.Models of trigeminal neurotomy were prepared through the excision of the frontal nerve and infraorbital nerve.Xiusanzhen acupuncture was delivered via electric stimulation.The acupoints selected were bilateral“Yingxiang(LI20)”and“Yintang(EX-HN3)”,and the stimulation parameters were as follows:disperse-dense wave,at a frequency of 2/15HZ and intensity of 1 mA.The course of treatment was once daily,with one course lasting five days,followed by an interval of two days,yielding a total of four courses.Behavioral changes were detected using the Morris water maze,changes in histomorphology in the hippocampal CA1 region were determined with hematoxylin and eosin(HE)staining,and MG activation in the hippocampal CA1 region was detected using immunofluorescence.Results:The escape latency:From days 3 to 5,the escape latency was higher in the model group compared to the sham-operation group(P<0.05),but was lower in the xiusanzhen group compared to the model and trigeminal neurotomy groups(P<0.05).The frequency for platform crossing and swimming distance:both were reduced in the model group compared to the sham-operation group(P<0.01);and were elevated in the xiusanzhen group compared to the model group and trigeminal neurotomy group(P<0.01).Hippocampal pathomorphological changes:In the sham-operation group,the morphological structure and nucleoli were well-defined;in the model group and the trigeminal neurotomy group,cell numbers were reduced and karyopyknosis increased;while in the xiusanzhen group,the cell numbers were elevated and karyopyknosis was reduced compared with the model group.MG:the positive rate was higher in the model group compared to the sham-operation group(P<0.05);lower in the xiusanzhen group compared to the model group(P<0.05);and higher in the trigeminal neurotomy group compared to the xiusanzhen group(P<0.05).Conclusion:Xiusanzhen acupuncture attenuates cognitive impairment in VD rats,inhibits MG activation,and reduces hippocampal tissue damage.Its effects depend on the structural integrity of the trigeminal nerve.
文摘Objective To observe the effects of Xiusanzhen treatment on the ultrastructure of olfactory bulb and the expression of substantia nigra glial fibrillary acidic protein(GFAP)in mice with Parkinson's disease(PD)induced by lipopolysaccharide(LPS),and to provide methods and evidence for early prevention and treatment of PD.Methods Forty C57BL/6 male mice were randomly divided into a blank group,a model group,an electroacupuncture(EA)group and a medication group,10 mice in each one.The mice in the model group,EA group and medication group were treated with 30-day nasal perfusion of LPS to establish PD model.From the first day of model establishment,the mice in the EA group were treated with electroacupuncture at bilateral“Yingxiang”(LI 20)and“Yintang”(GV 29)for 20 min,once a day;5-day treatment was taken as one session,and 4 sessions were given with an interval of 2 days between sessions.The mice in the medication group were treated with intraperitoneal injection of L-DOPA,10 mg/mL,once a day;5-day treatment was taken as one session,and 4 sessions were given with an interval of 2 days between sessions.After treatment,the behavioristics changes were observed by using footprint analysis and swimming test score;the ultrastructure of olfactory bulb was observed by using transmission electron microscopy;the expression of GFAP in substantia nigra was measured by using western blot method.Results ① After model establishment,the mice in the model group,the EA group and medication group showed significant symptoms of quiver and fear of chill,and the BMI was significantly lower than that in the blank group(all P<0.05).The results of behavioristics test indicated footprint length and swimming time in the model group,the EA group and medication group was significantly lower than those in the blank group(all P<0.01),indicating the successful establishment of PD model.② After onesession treatment,the symptoms of quiver and fear of chill were not observed in the EA group.After 4-session treatment,the BMI in the EA group was significantly higher than that in the model group(P<0.05);the symptoms of quiver and fear of chill were not observed in the medication group,but the BMI was similar with the model group(P>0.05).③ After treatment,the footprint and swimming time in the model group were significantly lower than that in the blank group(both P<0.01);the footprint and swimming time in the EA group and medication group were significantly higher than those in the model group(all P<0.01).④ After treatment,compared with the blank group,the organelles and ultrastructure of olfactory bulb in the model group were significantly improved;the ultrastructure of olfactory bulb in the EA group was improved compared with that in the model group.⑤ After treatment,the expression of substantia nigra GFAP in the model group was significantly higher than that in the blank group(P<0.01);the expression of substantia nigra GFAP in the EA group and medication group was significantly lower than that in the model group(both P<0.05).Conclusion The early treatment of Xiusanzhen can improve behavioral disorders in LPS-induced early PD mice,and the mechanism may be related to the regulation of olfactory disorders and the expression of GFAP in substantia nigra.
