A simple, sensitive and accurate method based on high performance liquid chromato- graphy （HPLC） with diode array detector （DAD） was developed and validated for systematic quality evaluation of one type of traditi...A simple, sensitive and accurate method based on high performance liquid chromato- graphy （HPLC） with diode array detector （DAD） was developed and validated for systematic quality evaluation of one type of traditional Chinese medicine preparations named Xinkeshu （XKS） tablet. In this study, the chromatographic fingerprints of XKS tablet were developed first, 23 peaks were selected as the common peaks to evaluate the similarities among different batches of XKS samples, which were manufactured in a long time span of three years. Additionally, simultaneous quantification of six markers in XKS tablet, including Danshensu, Protocatechuic aldehyde, Puerarin, Daidzin, Salvianolic acid B and Daidzein, was performed. The validation results showed that the developed method was specific, accurate, precise and robust. The preliminary explanation on why a close similarity between fingerprints did not exactly mean similar contents of chemical components in samples was given. The contribution of each chromatographic peak to similarity was also evaluated. The developed method offers an efficient, reliable and practical approach for systematic quality evaluation of XKS tablet.展开更多
OBJECTIVE:To investigate the clinical effects of Xinkeshu combined with levosimendan on perioperative heart failure in oldest-old patients with hip fractures.METHODS:Oldest-old patients over 80 years old with perioper...OBJECTIVE:To investigate the clinical effects of Xinkeshu combined with levosimendan on perioperative heart failure in oldest-old patients with hip fractures.METHODS:Oldest-old patients over 80 years old with perioperative heart failure and hip fractures were randomly divided into the control and observation groups,with 50 patients in each group.All patients in both groups were treated with conventional anti-heart failure therapy and levosimendan,whereas patients in the observation group additionally received Xinkeshu tablets.Clinical manifestations;left ventricular ejection fraction(LVEF);left ventricular end-diastolic dimension(LVEDD);left ventricular end-systolic dimension(LVESD);B-type natriuretic peptide(BNP),superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO),and endothelin-1(ET-1)levels;and self-rating anxiety scale(SAS)and self-rating depression scale(SDS)scores were compared between before and after treatment to evaluate the curative effects of Xinkeshu combined with levosimendan.RESULTS:After treatment,the efficacy rate was significantly higher in the observation group than in the control group.LVEF and the levels of SOD and NO were higher in the observation group than in the control group after treatment.However,LVEDD;LVESD;BNP,MDA,and ET-1 levels;and the SAS and SDS scores were lower after treatment in the observation group than in the control group.CONCLUSION:Levosimendan combined with Xinkeshu can improve cardiac function,alleviate oxidative stress,and relieve anxiety and depression in oldest-old patients with perioperative heart failure and hip fracture.展开更多
A reversed-phase high performance liquid chromatographic method for simultaneous determination of four bioactive constituents was established for the quality control of traditional Chinese medicinal tablet Xinkeshu. D...A reversed-phase high performance liquid chromatographic method for simultaneous determination of four bioactive constituents was established for the quality control of traditional Chinese medicinal tablet Xinkeshu. Danshensu, protocatechualdehyde, puerarin and daidzein were successfully separated on a Diamonsil C18 column (150×4.6 mm i.d., 5 祄) with a guard column (12.5×2.1 mm i.d., 5 祄) packed with the same material at 25 ℃. The mobile phase was a mixture of acetonitrile and 0.02 mol/L potassium dihydrogen phosphate employing gradi-ent elution at a flow rate of 0.8 mL/min. Detection was accomplished with a diode-array detector and detection wavelength was set at 280 nm before 22 min, then it was varied to 250 nm. The four constituents were identified by comparing their retention time and UV spectra in the range of 190—400 nm with those of authentic standards. The linear calibration ranges were 15.4—123.2, 3.11—25.22, 21.6—172.8, and 0.26—3.47 礸/mL for danshensu, pro-tocatechualdehyde, puerarin and daidzein, respectively. The detection limits were 0.03, 0.04, 0.10, 0.03 礸/mL for danshensu, protocatechualdehyde, puerarin, and daidzein, respectively. The contents of these four bioactive con-stituents in Xinkeshu tablet were successfully determined by the proposed method.展开更多
目的:建立一种操作简便、可行的 HPLC 法同时测定心可舒制剂中葛根素和丹酚酸 B 的含量。方法:色谱柱:SHIMAD-ZU Shim-pack VP-ODS(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸,梯度洗脱:0～15 min 时乙腈-0.1%磷酸比例为13:87,15...目的:建立一种操作简便、可行的 HPLC 法同时测定心可舒制剂中葛根素和丹酚酸 B 的含量。方法:色谱柱:SHIMAD-ZU Shim-pack VP-ODS(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸,梯度洗脱:0～15 min 时乙腈-0.1%磷酸比例为13:87,15～45 min 时比例为24:76;流速:1 mL·min^(-1);检测波长:305 nm。结果:葛根素进样量在0.0252～0.5040 μg范围内与峰面积线性关系良好(r=0.9999);丹酚酸 B 进样量在0.0154～0.308μg范围内与峰面积线性关系良好(r=0.9999)。心可舒胶囊中葛根素和丹酚酸 B 平均回收率分别为99.34%和99.57%,心可舒片中葛根素和丹酚酸 B 平均回收率分别为99.08%和100.0%。结论:采用 HPLC 法测定心可舒制剂中葛根素和丹酚酸 B 的含量,样品处理方法简便,测定结果准确,方法专属性强,精密度高,重复性好。展开更多
目的采用"Cocktail"探针药物法考察丹参注射液和心可舒片对家兔CYP酶5种亚型CYP1A2、CYP2C9、CYP2C19、CYP2D6和CYP3A4代谢的影响。方法将16只雄性家兔同时空腹给予混合探针药咖啡因、甲苯磺丁脲、奥美拉唑、右美沙芬和咪达唑...目的采用"Cocktail"探针药物法考察丹参注射液和心可舒片对家兔CYP酶5种亚型CYP1A2、CYP2C9、CYP2C19、CYP2D6和CYP3A4代谢的影响。方法将16只雄性家兔同时空腹给予混合探针药咖啡因、甲苯磺丁脲、奥美拉唑、右美沙芬和咪达唑仑,采用LC-MS/MS检测特定时间点的探针药物代谢率(代谢产物浓度与探针原型药物浓度的比值)。家兔随机分为丹参注射液组和心可舒片组,设计为自身对照实验,分3个周期,按顺序分别给药丹参低剂量组(0.5 m L·kg-1)、中剂量组(1 m L·kg-1)、高剂量组(2 m L·kg-1)和心可舒低剂量组(90 mg·kg-1)、中剂量组(180 mg·kg-1)和高剂量组(360 mg·kg-1)。每个周期经7 d洗净期后,连续给药7 d,第15天再给予探针药物后测代谢率。采用ANOVA方差分析比较各剂量组家兔和用药前探针药物的代谢率的变化。结果与用药前相比,各剂量组的混合探针药物代谢率均有下降趋势。结论丹参注射液、心可舒片使用7 d后可能抑制家兔体内CYP450酶代谢,与体外实验结果一致。这些药物与经过CYP450酶代谢的药物联合应用时,可能会引起药物相互作用,临床联合使用应提高警惕。展开更多
基金supported by the Major Projects of Independent Innovation Achievements of Shandong Province(No. 2010ZDZX1A0406)partly by the Scientific and Technological Projects of Shandong Province (No. 2009GG10002081)+1 种基金Independent Innovation Foundation of Shandong University(No. 2010TS054)Shandong Province Natural Science Foundation, China (No. ZR2011HM080)
文摘A simple, sensitive and accurate method based on high performance liquid chromato- graphy （HPLC） with diode array detector （DAD） was developed and validated for systematic quality evaluation of one type of traditional Chinese medicine preparations named Xinkeshu （XKS） tablet. In this study, the chromatographic fingerprints of XKS tablet were developed first, 23 peaks were selected as the common peaks to evaluate the similarities among different batches of XKS samples, which were manufactured in a long time span of three years. Additionally, simultaneous quantification of six markers in XKS tablet, including Danshensu, Protocatechuic aldehyde, Puerarin, Daidzin, Salvianolic acid B and Daidzein, was performed. The validation results showed that the developed method was specific, accurate, precise and robust. The preliminary explanation on why a close similarity between fingerprints did not exactly mean similar contents of chemical components in samples was given. The contribution of each chromatographic peak to similarity was also evaluated. The developed method offers an efficient, reliable and practical approach for systematic quality evaluation of XKS tablet.
文摘OBJECTIVE:To investigate the clinical effects of Xinkeshu combined with levosimendan on perioperative heart failure in oldest-old patients with hip fractures.METHODS:Oldest-old patients over 80 years old with perioperative heart failure and hip fractures were randomly divided into the control and observation groups,with 50 patients in each group.All patients in both groups were treated with conventional anti-heart failure therapy and levosimendan,whereas patients in the observation group additionally received Xinkeshu tablets.Clinical manifestations;left ventricular ejection fraction(LVEF);left ventricular end-diastolic dimension(LVEDD);left ventricular end-systolic dimension(LVESD);B-type natriuretic peptide(BNP),superoxide dismutase(SOD),malondialdehyde(MDA),nitric oxide(NO),and endothelin-1(ET-1)levels;and self-rating anxiety scale(SAS)and self-rating depression scale(SDS)scores were compared between before and after treatment to evaluate the curative effects of Xinkeshu combined with levosimendan.RESULTS:After treatment,the efficacy rate was significantly higher in the observation group than in the control group.LVEF and the levels of SOD and NO were higher in the observation group than in the control group after treatment.However,LVEDD;LVESD;BNP,MDA,and ET-1 levels;and the SAS and SDS scores were lower after treatment in the observation group than in the control group.CONCLUSION:Levosimendan combined with Xinkeshu can improve cardiac function,alleviate oxidative stress,and relieve anxiety and depression in oldest-old patients with perioperative heart failure and hip fracture.
基金Project supported by 985 Foundation of Chinese Traditional Medicine Modernization of Peking University.
文摘A reversed-phase high performance liquid chromatographic method for simultaneous determination of four bioactive constituents was established for the quality control of traditional Chinese medicinal tablet Xinkeshu. Danshensu, protocatechualdehyde, puerarin and daidzein were successfully separated on a Diamonsil C18 column (150×4.6 mm i.d., 5 祄) with a guard column (12.5×2.1 mm i.d., 5 祄) packed with the same material at 25 ℃. The mobile phase was a mixture of acetonitrile and 0.02 mol/L potassium dihydrogen phosphate employing gradi-ent elution at a flow rate of 0.8 mL/min. Detection was accomplished with a diode-array detector and detection wavelength was set at 280 nm before 22 min, then it was varied to 250 nm. The four constituents were identified by comparing their retention time and UV spectra in the range of 190—400 nm with those of authentic standards. The linear calibration ranges were 15.4—123.2, 3.11—25.22, 21.6—172.8, and 0.26—3.47 礸/mL for danshensu, pro-tocatechualdehyde, puerarin and daidzein, respectively. The detection limits were 0.03, 0.04, 0.10, 0.03 礸/mL for danshensu, protocatechualdehyde, puerarin, and daidzein, respectively. The contents of these four bioactive con-stituents in Xinkeshu tablet were successfully determined by the proposed method.
文摘目的:建立一种操作简便、可行的 HPLC 法同时测定心可舒制剂中葛根素和丹酚酸 B 的含量。方法:色谱柱:SHIMAD-ZU Shim-pack VP-ODS(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸,梯度洗脱:0～15 min 时乙腈-0.1%磷酸比例为13:87,15～45 min 时比例为24:76;流速:1 mL·min^(-1);检测波长:305 nm。结果:葛根素进样量在0.0252～0.5040 μg范围内与峰面积线性关系良好(r=0.9999);丹酚酸 B 进样量在0.0154～0.308μg范围内与峰面积线性关系良好(r=0.9999)。心可舒胶囊中葛根素和丹酚酸 B 平均回收率分别为99.34%和99.57%,心可舒片中葛根素和丹酚酸 B 平均回收率分别为99.08%和100.0%。结论:采用 HPLC 法测定心可舒制剂中葛根素和丹酚酸 B 的含量,样品处理方法简便,测定结果准确,方法专属性强,精密度高,重复性好。
文摘目的采用"Cocktail"探针药物法考察丹参注射液和心可舒片对家兔CYP酶5种亚型CYP1A2、CYP2C9、CYP2C19、CYP2D6和CYP3A4代谢的影响。方法将16只雄性家兔同时空腹给予混合探针药咖啡因、甲苯磺丁脲、奥美拉唑、右美沙芬和咪达唑仑,采用LC-MS/MS检测特定时间点的探针药物代谢率(代谢产物浓度与探针原型药物浓度的比值)。家兔随机分为丹参注射液组和心可舒片组,设计为自身对照实验,分3个周期,按顺序分别给药丹参低剂量组(0.5 m L·kg-1)、中剂量组(1 m L·kg-1)、高剂量组(2 m L·kg-1)和心可舒低剂量组(90 mg·kg-1)、中剂量组(180 mg·kg-1)和高剂量组(360 mg·kg-1)。每个周期经7 d洗净期后,连续给药7 d,第15天再给予探针药物后测代谢率。采用ANOVA方差分析比较各剂量组家兔和用药前探针药物的代谢率的变化。结果与用药前相比,各剂量组的混合探针药物代谢率均有下降趋势。结论丹参注射液、心可舒片使用7 d后可能抑制家兔体内CYP450酶代谢,与体外实验结果一致。这些药物与经过CYP450酶代谢的药物联合应用时,可能会引起药物相互作用,临床联合使用应提高警惕。
