Objective To explore the medication patterns of Professor Zulong Wang in treating sleep-related painful erection(SRPE)through data mining,aiming to discover new therapeutic strategies for traditional Chinese medicine ...Objective To explore the medication patterns of Professor Zulong Wang in treating sleep-related painful erection(SRPE)through data mining,aiming to discover new therapeutic strategies for traditional Chinese medicine in managing this condition.Methods Medical records of SRPE patients treated by Professor Zulong Wang at the First Affiliated Hospital of Henan University of Chinese Medicine from February 2021 to December 2024 were collected to establish a database.Frequency analysis,association rule analysis,and cluster analysis were employed to examine the frequency of herb use,four properties and five flavors,channel tropisms,and herb combinations.Results A total of 59 prescriptions meeting the criteria were included,involving 81 Chinese herbs,among which 27 herbs were used 10 or more times.The primary herb properties were cold,warm,and neutral,with bitter,sweet,and pungent flavors being most common.The herbs predominantly entered the liver,heart,spleen,and kidney channels.Forty-three commonly used herb combinations were identified,and cluster analysis revealed six core herb groups.Conclusion Professor Zulong Wang primarily treats SRPE with modified Huoluo Xiaoling Dan,focusing on unblocking collaterals and relieving pain,supplemented by soothing the liver and regulating qi,resolving stasis and phlegm,nourishing yin and reducing fire,and tonifying the kidney and activating blood circulation.展开更多
Objective This study aimed to compare the mechanisms and efficacies of five formulas that improve blood circulation and remove blood stasis.Methods(1)A network pharmacology method was used to determine the targets of ...Objective This study aimed to compare the mechanisms and efficacies of five formulas that improve blood circulation and remove blood stasis.Methods(1)A network pharmacology method was used to determine the targets of five formulas that promote circulation and remove blood stasis.Compounds of the five formulas,namely Danshen Yin(丹参饮,DSY),Huoluo Xiaoling Dan(活络效灵丹,HLXLD),Shixiao San(失笑散,SXS),Taohong Siwu Tang(桃红四物汤,THSWT),and Xuefu Zhuyu Tang(血府逐瘀汤,XFZYT),were retrieved from the Traditonal Chinese Medicine System Pharmacology Database(TCMSP),the Shanghai Institute of Organic Chemistry of CAS,and the TCM Integrated Database.Drug target network was constructed by searching the Swiss Target Prediction database and the STITCH database.The target network of stasis was extracted from the PharmGKB database,the Online Mendelian Inheritance in Man(OMIM)database,the Genetic Association Database(GAD),and the Therapeutic Target Database(TTD).Candidate targets were determined using protein-protein interaction(PPI)network extension and topology selection.Thereafter,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis was used to determine the differentiation of the mechanism of the five formulas.(2)Animal experiments were conducted to explore the efficacies of the five formulas in treating blood stasis.Seventy New Zealand rabbits were exposed to high-fat feeding+epinephrine injection to construct a blood stasis syndrome model.The rabbits were evenly divided into control,model,DSY,HLXLD,SXS,THSWT,and XFZYT groups.The latter five groups were orally administered the corresponding formulas[DSY:3.92 g/(kg·d),XFZYT:7.10 g/(kg·d),SXS:1.12 g/(kg·d),HLXLD:5.60 g/(kg·d),THSWT:4.48 g/(kg·d)].Serum lipid and blood rheology were analyzed,and pathology slices were observed.Results(1)A total of 269,358,288,370,and 376 candidate targets of DSY,HLXLD,SXS,THSWT,and XFZYT were obtained among which were 232 shared candidate targets.Fluid shear stress and atherosclerosis were the biological processes common to the five formulas.HLXLD,SXS,DSY,and THSWT regulated lipolysis in adipocytes,and XFZYT,HLXLD,SXS,and THSWT regulated the AGE-RAGE signaling pathway and complement and coagulation cascades.HLXLD,SXS,and XFZYT regulated the HIF-1 signaling pathway,DSY regulated the cGMP-PKG signaling pathway,HLXLD reduced platelet activation,SXS regulated the calcium signaling pathway,and XFZYT regulated the PPAR signaling pathway.(2)In the animal experiments,the values of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL),high density lipoprotein cholesterol(HDL),TC/HDL,and TG/HDL in each group decreased,among which the ones seen in XFZYT,HLXLD,and SXS groups were statistically significant(P<0.05).XFZYT presented the best effect,followed by HLXLD and SXS.XFZYT and HLXLD decreased apolipoprotein B100(apoB100)and increased apolipoprotein A1/apoB100(P<0.05).XFZYT decreased all the values of hematocrit(HCT),plasma viscosity,whole blood viscosity(WBV);HLXLD and SXS affected HCT;and DSY and THSWT regulated WBV(P<0.05).All the five formulas decreased the values of optical density and area of plaque,among which XFZYT and HLXLD showed statistical significance(P<0.05).Conclusion Adjusting fluid shear stress and alleviating the injury of endothelial cells might be the common mechanisms by which the five formulas promote blood circulation and remove blood stasis.Different formulas also have unique targets,which may provide guidance for clinical drug selection.By regulating different indices,the five formulas can regulate blood lipid and hemorheology,improve the state of blood stasis,and decrease the degree of aortic plaque in the blood stasis model rabbits.XFZYT and HLXLD had higher efficacies than DSY,THSWT,and SXS.展开更多
Dominated by an arid and semiarid continental climate,the Beijing-Tianjin Sandstorm Source Region(BTSSR)is a typical ecologically fragile region with frequently occurring droughts.To provide information for regional v...Dominated by an arid and semiarid continental climate,the Beijing-Tianjin Sandstorm Source Region(BTSSR)is a typical ecologically fragile region with frequently occurring droughts.To provide information for regional vegetation protection and drought prevention,we assessed the relations between vegetation cover change(measured by the Normalized Difference Vegetation Index,NDVI)and the Standardized Precipitation Evapotranspiration Index(SPEI)at different time-scales,in different growth stages,in different subregions and for different vegetation types based on the Pearson's correlation coefficient in the BTSSR from 2000 to 2017.Results showed that 88.19%of the vegetated areas experienced increased NDVI in the growing season;48.3%of the vegetated areas experi-enced significantly increased NDVI(P<0.05)and were mainly in the south of the BTSSR.During the growing season,a wetter climate contributed to the increased vegetation cover from 2000 to 2017,and NDVI anomalies were closely related to SPEI.The maximum correlation coefficient in the growing season(Rmax)was significantly positive(P<0.05)in 97.84%of the total vegetated areas.In the vegetated areas with significantly positive Rmax,pixels with short time-scales(1-3 mon)accounted for the largest proportion(33.9%).The sensitivity of vegetation to the impact of drought rose first and then decreased in the growing season,with a peak in July.Compared with two subregions in the south,subregions in the north of the BTSSR were more sensitive to the impacts of drought variations,especially in the Xilingol Plateau and Wuzhumuqin Basin.All four major vegetation types were sensitive to the effects of drought variations,especially grasslands.The time-scales of the most impacting droughts varied with growth stages,regions,and vegetation types.These results can help us understand the relations between vegetation and droughts,which are important for ecological restoration and drought prevention.展开更多
Objective: To analyze the possible mechanism of the Huoluo Xiaoling Pill in the treatment of three diseases, hyperglycemia, hyperlipidemia, and metabolic syndrome, and to provide ideas for learning the mechanism of “...Objective: To analyze the possible mechanism of the Huoluo Xiaoling Pill in the treatment of three diseases, hyperglycemia, hyperlipidemia, and metabolic syndrome, and to provide ideas for learning the mechanism of “Treating different diseases with the same method” in Traditional Chinese Medicine(TCM) theory. Materials and Methods: The Traditional Chinese Medicine System Pharmacology Database and Uni Prot databases were used to screen the main ingredients and targets of the Huoluo Xiaoling Pill. The Gene Cards database was used to screen the targets of the diseases, and Cytoscape 3.7.2 was used to construct a “Drug-Components-Targets-Disease” network to determine the core components. The STRING database was used to construct the protein-protein-interaction network, and gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomics enrichment analyses were carried out on the Metascape database. Auto Dock 1.5.6 was used for molecular docking. Results: A total of 118 active components and 208 targets were screened in the Huoluo Xiaoling Pill. Quercetin, tanshinone IIA, luteolin, and ellagic acid were potential core components of Huoluo Xiaoling Pill treating the three diseases, and interleukin 6, Tumor necrosis factor, and vascular endothelial growth factor were potential key targets. Co-occurring GO biological processes involved responses to the molecules of bacterial origin, and the AGE-RAGE signaling, fluid shear stress, and atherosclerosis pathways were the co-occurring pathways. Molecular docking revealed good docking conditions between screened targets and components. Conclusion: This study predicted the mechanism of the Huoluo Xiaoling Pill in treating the three diseases. At the same time, the co-occurring targets and pathways between the three diseases provided a material basis for the TCM theory, “Treating different diseases with the same method.”展开更多
基金supported by the National Natural Science Foundation of China General Program(82174377)National Traditional Chinese Medicine Advantageous Specialty Construction Project(GZY-ZY[2024]90).
文摘Objective To explore the medication patterns of Professor Zulong Wang in treating sleep-related painful erection(SRPE)through data mining,aiming to discover new therapeutic strategies for traditional Chinese medicine in managing this condition.Methods Medical records of SRPE patients treated by Professor Zulong Wang at the First Affiliated Hospital of Henan University of Chinese Medicine from February 2021 to December 2024 were collected to establish a database.Frequency analysis,association rule analysis,and cluster analysis were employed to examine the frequency of herb use,four properties and five flavors,channel tropisms,and herb combinations.Results A total of 59 prescriptions meeting the criteria were included,involving 81 Chinese herbs,among which 27 herbs were used 10 or more times.The primary herb properties were cold,warm,and neutral,with bitter,sweet,and pungent flavors being most common.The herbs predominantly entered the liver,heart,spleen,and kidney channels.Forty-three commonly used herb combinations were identified,and cluster analysis revealed six core herb groups.Conclusion Professor Zulong Wang primarily treats SRPE with modified Huoluo Xiaoling Dan,focusing on unblocking collaterals and relieving pain,supplemented by soothing the liver and regulating qi,resolving stasis and phlegm,nourishing yin and reducing fire,and tonifying the kidney and activating blood circulation.
基金We thank for the funding support from the Special Project of Central Government Guiding Local Science and Technology Development(No.2019XF5062)the 211 Project of Chinese Medicine Diagnostics(No.80019992)the Open Fund of National Key Discipline of Chinese Medicine Diagnostics of Hunan University of Chinese Medicine(No.ZZKF201501 and No.2015ZYZD01).
文摘Objective This study aimed to compare the mechanisms and efficacies of five formulas that improve blood circulation and remove blood stasis.Methods(1)A network pharmacology method was used to determine the targets of five formulas that promote circulation and remove blood stasis.Compounds of the five formulas,namely Danshen Yin(丹参饮,DSY),Huoluo Xiaoling Dan(活络效灵丹,HLXLD),Shixiao San(失笑散,SXS),Taohong Siwu Tang(桃红四物汤,THSWT),and Xuefu Zhuyu Tang(血府逐瘀汤,XFZYT),were retrieved from the Traditonal Chinese Medicine System Pharmacology Database(TCMSP),the Shanghai Institute of Organic Chemistry of CAS,and the TCM Integrated Database.Drug target network was constructed by searching the Swiss Target Prediction database and the STITCH database.The target network of stasis was extracted from the PharmGKB database,the Online Mendelian Inheritance in Man(OMIM)database,the Genetic Association Database(GAD),and the Therapeutic Target Database(TTD).Candidate targets were determined using protein-protein interaction(PPI)network extension and topology selection.Thereafter,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis was used to determine the differentiation of the mechanism of the five formulas.(2)Animal experiments were conducted to explore the efficacies of the five formulas in treating blood stasis.Seventy New Zealand rabbits were exposed to high-fat feeding+epinephrine injection to construct a blood stasis syndrome model.The rabbits were evenly divided into control,model,DSY,HLXLD,SXS,THSWT,and XFZYT groups.The latter five groups were orally administered the corresponding formulas[DSY:3.92 g/(kg·d),XFZYT:7.10 g/(kg·d),SXS:1.12 g/(kg·d),HLXLD:5.60 g/(kg·d),THSWT:4.48 g/(kg·d)].Serum lipid and blood rheology were analyzed,and pathology slices were observed.Results(1)A total of 269,358,288,370,and 376 candidate targets of DSY,HLXLD,SXS,THSWT,and XFZYT were obtained among which were 232 shared candidate targets.Fluid shear stress and atherosclerosis were the biological processes common to the five formulas.HLXLD,SXS,DSY,and THSWT regulated lipolysis in adipocytes,and XFZYT,HLXLD,SXS,and THSWT regulated the AGE-RAGE signaling pathway and complement and coagulation cascades.HLXLD,SXS,and XFZYT regulated the HIF-1 signaling pathway,DSY regulated the cGMP-PKG signaling pathway,HLXLD reduced platelet activation,SXS regulated the calcium signaling pathway,and XFZYT regulated the PPAR signaling pathway.(2)In the animal experiments,the values of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL),high density lipoprotein cholesterol(HDL),TC/HDL,and TG/HDL in each group decreased,among which the ones seen in XFZYT,HLXLD,and SXS groups were statistically significant(P<0.05).XFZYT presented the best effect,followed by HLXLD and SXS.XFZYT and HLXLD decreased apolipoprotein B100(apoB100)and increased apolipoprotein A1/apoB100(P<0.05).XFZYT decreased all the values of hematocrit(HCT),plasma viscosity,whole blood viscosity(WBV);HLXLD and SXS affected HCT;and DSY and THSWT regulated WBV(P<0.05).All the five formulas decreased the values of optical density and area of plaque,among which XFZYT and HLXLD showed statistical significance(P<0.05).Conclusion Adjusting fluid shear stress and alleviating the injury of endothelial cells might be the common mechanisms by which the five formulas promote blood circulation and remove blood stasis.Different formulas also have unique targets,which may provide guidance for clinical drug selection.By regulating different indices,the five formulas can regulate blood lipid and hemorheology,improve the state of blood stasis,and decrease the degree of aortic plaque in the blood stasis model rabbits.XFZYT and HLXLD had higher efficacies than DSY,THSWT,and SXS.
基金Under the auspices of National Natural Science Foundation of China(No.41807177,41701017)the Pioneer‘Hundred Talents Program’of Chinese Academy of Sciences。
文摘Dominated by an arid and semiarid continental climate,the Beijing-Tianjin Sandstorm Source Region(BTSSR)is a typical ecologically fragile region with frequently occurring droughts.To provide information for regional vegetation protection and drought prevention,we assessed the relations between vegetation cover change(measured by the Normalized Difference Vegetation Index,NDVI)and the Standardized Precipitation Evapotranspiration Index(SPEI)at different time-scales,in different growth stages,in different subregions and for different vegetation types based on the Pearson's correlation coefficient in the BTSSR from 2000 to 2017.Results showed that 88.19%of the vegetated areas experienced increased NDVI in the growing season;48.3%of the vegetated areas experi-enced significantly increased NDVI(P<0.05)and were mainly in the south of the BTSSR.During the growing season,a wetter climate contributed to the increased vegetation cover from 2000 to 2017,and NDVI anomalies were closely related to SPEI.The maximum correlation coefficient in the growing season(Rmax)was significantly positive(P<0.05)in 97.84%of the total vegetated areas.In the vegetated areas with significantly positive Rmax,pixels with short time-scales(1-3 mon)accounted for the largest proportion(33.9%).The sensitivity of vegetation to the impact of drought rose first and then decreased in the growing season,with a peak in July.Compared with two subregions in the south,subregions in the north of the BTSSR were more sensitive to the impacts of drought variations,especially in the Xilingol Plateau and Wuzhumuqin Basin.All four major vegetation types were sensitive to the effects of drought variations,especially grasslands.The time-scales of the most impacting droughts varied with growth stages,regions,and vegetation types.These results can help us understand the relations between vegetation and droughts,which are important for ecological restoration and drought prevention.
文摘Objective: To analyze the possible mechanism of the Huoluo Xiaoling Pill in the treatment of three diseases, hyperglycemia, hyperlipidemia, and metabolic syndrome, and to provide ideas for learning the mechanism of “Treating different diseases with the same method” in Traditional Chinese Medicine(TCM) theory. Materials and Methods: The Traditional Chinese Medicine System Pharmacology Database and Uni Prot databases were used to screen the main ingredients and targets of the Huoluo Xiaoling Pill. The Gene Cards database was used to screen the targets of the diseases, and Cytoscape 3.7.2 was used to construct a “Drug-Components-Targets-Disease” network to determine the core components. The STRING database was used to construct the protein-protein-interaction network, and gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomics enrichment analyses were carried out on the Metascape database. Auto Dock 1.5.6 was used for molecular docking. Results: A total of 118 active components and 208 targets were screened in the Huoluo Xiaoling Pill. Quercetin, tanshinone IIA, luteolin, and ellagic acid were potential core components of Huoluo Xiaoling Pill treating the three diseases, and interleukin 6, Tumor necrosis factor, and vascular endothelial growth factor were potential key targets. Co-occurring GO biological processes involved responses to the molecules of bacterial origin, and the AGE-RAGE signaling, fluid shear stress, and atherosclerosis pathways were the co-occurring pathways. Molecular docking revealed good docking conditions between screened targets and components. Conclusion: This study predicted the mechanism of the Huoluo Xiaoling Pill in treating the three diseases. At the same time, the co-occurring targets and pathways between the three diseases provided a material basis for the TCM theory, “Treating different diseases with the same method.”
