Objective:To explore the antibacterial mechanism of the active components of Xiangsu powder through the network pharmacological approach.Methods:TCMSP database was used to search and obtain the active components of Xi...Objective:To explore the antibacterial mechanism of the active components of Xiangsu powder through the network pharmacological approach.Methods:TCMSP database was used to search and obtain the active components of Xiangsu powder and its corresponding action targets,and its network relationship was defined.Target points associated with antibacterial effect were searched in Genecards database,and core target genes of antibacterial effect were obtained by mnapping the target points..Finally,the GO biological process and KEGG metabolic pathway were analyzed in the DAVID database.Results:There were 129 active components,250 targets,and 66 biological processes(40 biological processes,13 molcular functions,13 cell compositions)and 35 related signaling pathways.Among theim,quercetin may be the main substance that plays a role in the drugs contained in Xiangsu powder,followed by flavonol kaempferol and favonoid luteolin.Antibacterial targets such as TNF,Casp3,PTGS2,ACTB,STAT1 and NFKB1 are mostly involved in antiviral,anti-inflammatory and immune pathways.It mainly plays a role in the hepatitis B pathway and tunor necrosis factor signaling pathway.Conclusion:Quercetin,kaempferol,luteolin and other active components in Xiangsu powder can participate in the action process of Toll-like receptor pathway,TNF signaling pathway and other pathways through acting on TNF,Casp3,PTGS2,etc.,and finally achieve the purpose of antibacterial.展开更多
Background:This paper investigates the anti-tumor mechanism of action of Poria cocos on the basis of network pharmacology.Method:In this paper,we screen the potential active ingredients of Poria cocos by TCMSP and obt...Background:This paper investigates the anti-tumor mechanism of action of Poria cocos on the basis of network pharmacology.Method:In this paper,we screen the potential active ingredients of Poria cocos by TCMSP and obtain their corresponding targets with SwissTargetPrediction.The GeneCards and OMIM databases are used to screen the relevant pathogenic candidate targets in various tumor disease processes.Furthermore,we obtain Poria cocos-tumor common targets by taking the intersection of Poria cocos potential targets and candidate target groups.Subsequently,the protein-protein interaction network(PPI)of common target genes is mapped based on the STRING database,and the"drug-active component-target gene-disease"network is constructed with the help of Cytoscape3.7.2.Therefore,the core target genes are obtained.Finally,GO and pathway enrichment analysis of the core target genes are performed by Metascape and DAVID.Results:38 common targets and 7 core genes(ESR1,MAPK3,MAPK8,MTOR,PIK3CA,JAK2,and IL6)in Poria cocos-tumors are found.They play an anti-tumor role by regulating various classical pathways such as PI3K-Akt signaling pathway,mTOR signaling pathway,Prolactin signaling pathway,ErbB signaling pathway,Choline metabolism in cancer.Conclusion:The research reveals the effective anti-tumor function of Poria as a multi-component,multi-target and multi-pathway herbal medicine.展开更多
基金This study was supported by Jiangxi Innovation and Entrepreneurship Training Program for College Students(S202010412079)Study on the design of mask for TCM epidemic prevention based on incense therapy.
文摘Objective:To explore the antibacterial mechanism of the active components of Xiangsu powder through the network pharmacological approach.Methods:TCMSP database was used to search and obtain the active components of Xiangsu powder and its corresponding action targets,and its network relationship was defined.Target points associated with antibacterial effect were searched in Genecards database,and core target genes of antibacterial effect were obtained by mnapping the target points..Finally,the GO biological process and KEGG metabolic pathway were analyzed in the DAVID database.Results:There were 129 active components,250 targets,and 66 biological processes(40 biological processes,13 molcular functions,13 cell compositions)and 35 related signaling pathways.Among theim,quercetin may be the main substance that plays a role in the drugs contained in Xiangsu powder,followed by flavonol kaempferol and favonoid luteolin.Antibacterial targets such as TNF,Casp3,PTGS2,ACTB,STAT1 and NFKB1 are mostly involved in antiviral,anti-inflammatory and immune pathways.It mainly plays a role in the hepatitis B pathway and tunor necrosis factor signaling pathway.Conclusion:Quercetin,kaempferol,luteolin and other active components in Xiangsu powder can participate in the action process of Toll-like receptor pathway,TNF signaling pathway and other pathways through acting on TNF,Casp3,PTGS2,etc.,and finally achieve the purpose of antibacterial.
基金Fund project:Anhui University of Traditional Chinese Medicine Student Innovation and Entrepreneurship Project(No.202006170606)Natural Science Key Project of Anhui University of Traditional Chinese Medicine(No.2020zrzd15)National Natural Youth Science Foundation of China(No.81904062)。
文摘Background:This paper investigates the anti-tumor mechanism of action of Poria cocos on the basis of network pharmacology.Method:In this paper,we screen the potential active ingredients of Poria cocos by TCMSP and obtain their corresponding targets with SwissTargetPrediction.The GeneCards and OMIM databases are used to screen the relevant pathogenic candidate targets in various tumor disease processes.Furthermore,we obtain Poria cocos-tumor common targets by taking the intersection of Poria cocos potential targets and candidate target groups.Subsequently,the protein-protein interaction network(PPI)of common target genes is mapped based on the STRING database,and the"drug-active component-target gene-disease"network is constructed with the help of Cytoscape3.7.2.Therefore,the core target genes are obtained.Finally,GO and pathway enrichment analysis of the core target genes are performed by Metascape and DAVID.Results:38 common targets and 7 core genes(ESR1,MAPK3,MAPK8,MTOR,PIK3CA,JAK2,and IL6)in Poria cocos-tumors are found.They play an anti-tumor role by regulating various classical pathways such as PI3K-Akt signaling pathway,mTOR signaling pathway,Prolactin signaling pathway,ErbB signaling pathway,Choline metabolism in cancer.Conclusion:The research reveals the effective anti-tumor function of Poria as a multi-component,multi-target and multi-pathway herbal medicine.
