Objective:To study correlation between the Xba I polymorphism of apoB gene and plasma lipid profiles in Li ethnic group.Methods:Total 1S1 cases of healthy Li people were recruited randomly by cluster sampling and 200 ...Objective:To study correlation between the Xba I polymorphism of apoB gene and plasma lipid profiles in Li ethnic group.Methods:Total 1S1 cases of healthy Li people were recruited randomly by cluster sampling and 200 Han people were recruited as control;blood was drawn to analyze Xba I polymorphism distribution of apoB gene and serum lipid levels.Results:There were lower serum total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)levels in serum of Li people;while,high density lipoprotein cholesterol(HDL-C),X^-/X^-genotype and X^+allele frequencies exhibited higher levels than Han people.Interestingly,HDL-C level was reduced,while LDL-C level was enhanced in subjects carrying heterozygous(X^-/X^-)genotype compared to homozygous(X^-/X^-)genotype.Additionally,there were no difference in serum level of triglyceride,TC,apoprotein A(apo A)and apoprotein B(apo B)between Li and Han people,the same results were showed between X^-/X^+and X^-/X^-genotype carriers.Conclusions:XbaⅠpolymorphism of apoB gene is correlated to the profiles of serum lipid level,X^-/X^+genotype carriers are phenotyped with higher LDL-C level and lower level of HDL-C in Li ethnic group.展开更多
Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) gene...Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.展开更多
Objective: this study mainly discussed the relationship between the polymorphism of PuvⅡ and XbaⅠ gene of estrogen α receptor and benign paroxysmal positional vertigo (BPPV). Methods: polymerase chain reaction-rest...Objective: this study mainly discussed the relationship between the polymorphism of PuvⅡ and XbaⅠ gene of estrogen α receptor and benign paroxysmal positional vertigo (BPPV). Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the genotypes of 40 patients with benign positional vertigo and 40 healthy people, and the gene polypeptide of PvuⅡ (RS4986938) and XbaⅠ (RS9340799) was detected. SPSS13.0 statistical software was used to analyze the correlation between gene polypeptide of PvuⅡ and XbaⅠ and BPPV. Results: 1. There are three genotypes of PVU ⅱ, namely Pp, pp, PP, and the distribution frequency of genotypes between BPPV group and control group is statistically significant (28.498, p0.014). The frequency of P gene in PvuⅡ allele is statistically significant (29.452, p0.02). 2. There are three genotypes in the restriction site of XbaⅠ. There is no significant difference in the distribution frequency of Xba Ⅰ genotype between BPPV group and control group (21.953, P 0.377). There was no significant difference in the frequencies of P and P genes in XbaⅠ allele between BPPV group and control group (22.020, p0.155). 3. There are 9 combined genotypes of ERα PvuⅡ and XbaⅠ. There was no significant difference between the two groups (21.953 p0.377, P > 0.05).展开更多
基金supported by natural science foundation of Hainan province.Haikou,China(No.30726)
文摘Objective:To study correlation between the Xba I polymorphism of apoB gene and plasma lipid profiles in Li ethnic group.Methods:Total 1S1 cases of healthy Li people were recruited randomly by cluster sampling and 200 Han people were recruited as control;blood was drawn to analyze Xba I polymorphism distribution of apoB gene and serum lipid levels.Results:There were lower serum total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)levels in serum of Li people;while,high density lipoprotein cholesterol(HDL-C),X^-/X^-genotype and X^+allele frequencies exhibited higher levels than Han people.Interestingly,HDL-C level was reduced,while LDL-C level was enhanced in subjects carrying heterozygous(X^-/X^-)genotype compared to homozygous(X^-/X^-)genotype.Additionally,there were no difference in serum level of triglyceride,TC,apoprotein A(apo A)and apoprotein B(apo B)between Li and Han people,the same results were showed between X^-/X^+and X^-/X^-genotype carriers.Conclusions:XbaⅠpolymorphism of apoB gene is correlated to the profiles of serum lipid level,X^-/X^+genotype carriers are phenotyped with higher LDL-C level and lower level of HDL-C in Li ethnic group.
基金National Natural Sciences Foundation of China No. 39700165
文摘Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.
文摘Objective: this study mainly discussed the relationship between the polymorphism of PuvⅡ and XbaⅠ gene of estrogen α receptor and benign paroxysmal positional vertigo (BPPV). Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the genotypes of 40 patients with benign positional vertigo and 40 healthy people, and the gene polypeptide of PvuⅡ (RS4986938) and XbaⅠ (RS9340799) was detected. SPSS13.0 statistical software was used to analyze the correlation between gene polypeptide of PvuⅡ and XbaⅠ and BPPV. Results: 1. There are three genotypes of PVU ⅱ, namely Pp, pp, PP, and the distribution frequency of genotypes between BPPV group and control group is statistically significant (28.498, p0.014). The frequency of P gene in PvuⅡ allele is statistically significant (29.452, p0.02). 2. There are three genotypes in the restriction site of XbaⅠ. There is no significant difference in the distribution frequency of Xba Ⅰ genotype between BPPV group and control group (21.953, P 0.377). There was no significant difference in the frequencies of P and P genes in XbaⅠ allele between BPPV group and control group (22.020, p0.155). 3. There are 9 combined genotypes of ERα PvuⅡ and XbaⅠ. There was no significant difference between the two groups (21.953 p0.377, P > 0.05).
