[Objectives]To investigate the protective effects of xanthoxylin on acute lung injury induced by D-Galactosamine(D-GalN)and Lipopolysaccharide(LPS)in rats.[Methods]Sixty male SD rats were randomly divided into a norma...[Objectives]To investigate the protective effects of xanthoxylin on acute lung injury induced by D-Galactosamine(D-GalN)and Lipopolysaccharide(LPS)in rats.[Methods]Sixty male SD rats were randomly divided into a normal group,a model group,a silybin group(50 mg/kg),and three xanthoxylin groups(low-dose,medium-dose,high-dose as 60,120,and 240 mg/kg),10 rats per group.The rats were administered for 17 consecutive days,on day 14,all the rats except for the normal group were intraperitoneally injected with a D-GalN(400 mg/kg)/LPS(30μg/kg)mixture once to establish acute lung injury models.At 72 h after modeling,their serum MCP-1 levels,IL-1β,IL-6,PCT,CRP,TNF-αlevels in bronchoalveolar lavage fluid,and IL-1β,IL-6,TNF-αlevels in lung tissue were measured,and lung tissue histological examination were checked by HE staining.[Results]Compared with the model group,the serum MCP-1 levels,IL-1β,IL-6,PCT,CRP,TNF-αlevels in bronchoalveolar lavage fluid,and IL-1β,IL-6,TNF-αlevels in lung tissue in xanthoxylin groups were significantly decreased(P<0.05 or P<0.01),and lung tissue injury were alleviated.[Conclusions]Xanthoxylin has protective effects on acute lung injury in rats,and it may be related to the increase of anti-inflammatory capacity and the promotion of lung tissue self-healing.展开更多
提出从艾纳香叶中制取花椒油素的方案。通过同时蒸馏萃取法(SDE)提取花椒油素,利用硅胶柱层析分离粗品,经过重结晶纯化得高纯产品,通过气相色谱(GC)和核磁共振(NMR)对花椒油素进行定量和定性分析。在料水比1∶8,溶剂(无水乙醚)体积60 m...提出从艾纳香叶中制取花椒油素的方案。通过同时蒸馏萃取法(SDE)提取花椒油素,利用硅胶柱层析分离粗品,经过重结晶纯化得高纯产品,通过气相色谱(GC)和核磁共振(NMR)对花椒油素进行定量和定性分析。在料水比1∶8,溶剂(无水乙醚)体积60 m L,提取时间2.5 h的参数下通过SDE法提取花椒油素,GC测定显示粗提物中含量达20.26%。硅胶柱参数为洗脱液配比[V(乙酸乙酯)∶V(石油醚)=1∶19]、径高比1∶14、样品与硅胶质量比(上样量)1∶40、洗脱液流速3.5 m L/min时,可分离到花椒油素粗品(纯度72.09%),回收率为99.04%。经过3次重结晶得到高纯度产品(98.37%),总回收率为91.92%,得率为0.28%。NMR结果证实产品为花椒油素。展开更多
基金Supported by Scientific Research Start-up Fund for Introduced PhD.Talents of Guangxi University of Chinese Medicine,2023(2023BS024)Basic Research Ability Enhancement Project for Young and Middle-aged Teachers in Guangxi Universities(2022KY0286)+2 种基金Guangxi Key Research and Development Program(Guike AB21196016)Guangxi Science and Technology Base and Talent Special Project(Guike AD21238031)Guangxi Key Discipline of Zhuang Medicine in Chinese Medicine(GZXK-Z-20-64).
文摘[Objectives]To investigate the protective effects of xanthoxylin on acute lung injury induced by D-Galactosamine(D-GalN)and Lipopolysaccharide(LPS)in rats.[Methods]Sixty male SD rats were randomly divided into a normal group,a model group,a silybin group(50 mg/kg),and three xanthoxylin groups(low-dose,medium-dose,high-dose as 60,120,and 240 mg/kg),10 rats per group.The rats were administered for 17 consecutive days,on day 14,all the rats except for the normal group were intraperitoneally injected with a D-GalN(400 mg/kg)/LPS(30μg/kg)mixture once to establish acute lung injury models.At 72 h after modeling,their serum MCP-1 levels,IL-1β,IL-6,PCT,CRP,TNF-αlevels in bronchoalveolar lavage fluid,and IL-1β,IL-6,TNF-αlevels in lung tissue were measured,and lung tissue histological examination were checked by HE staining.[Results]Compared with the model group,the serum MCP-1 levels,IL-1β,IL-6,PCT,CRP,TNF-αlevels in bronchoalveolar lavage fluid,and IL-1β,IL-6,TNF-αlevels in lung tissue in xanthoxylin groups were significantly decreased(P<0.05 or P<0.01),and lung tissue injury were alleviated.[Conclusions]Xanthoxylin has protective effects on acute lung injury in rats,and it may be related to the increase of anti-inflammatory capacity and the promotion of lung tissue self-healing.
文摘提出从艾纳香叶中制取花椒油素的方案。通过同时蒸馏萃取法(SDE)提取花椒油素,利用硅胶柱层析分离粗品,经过重结晶纯化得高纯产品,通过气相色谱(GC)和核磁共振(NMR)对花椒油素进行定量和定性分析。在料水比1∶8,溶剂(无水乙醚)体积60 m L,提取时间2.5 h的参数下通过SDE法提取花椒油素,GC测定显示粗提物中含量达20.26%。硅胶柱参数为洗脱液配比[V(乙酸乙酯)∶V(石油醚)=1∶19]、径高比1∶14、样品与硅胶质量比(上样量)1∶40、洗脱液流速3.5 m L/min时,可分离到花椒油素粗品(纯度72.09%),回收率为99.04%。经过3次重结晶得到高纯度产品(98.37%),总回收率为91.92%,得率为0.28%。NMR结果证实产品为花椒油素。
