XPR1是最近鉴定出的一个原发性家族性脑钙化症(primary familial brian calcification,PFBC)的致病基因,它在果蝇体内的同源基因是CG10483(dXPR1).使用CRISPR/Cas9系统,构建原位表达dXPR1-GFP的果蝇品系,在CG10483终止密码子之前插入绿...XPR1是最近鉴定出的一个原发性家族性脑钙化症(primary familial brian calcification,PFBC)的致病基因,它在果蝇体内的同源基因是CG10483(dXPR1).使用CRISPR/Cas9系统,构建原位表达dXPR1-GFP的果蝇品系,在CG10483终止密码子之前插入绿色荧光蛋白(green fluorescent protein,GFP)的编码序列,使果蝇表达dXPR1-GFP融合蛋白.该融合蛋白既可以被CG10483编码蛋白的抗体和GFP的抗体识别又能在激发光下发出绿色荧光,能为dXPR1编码蛋白提供准确的定位,从而为研究XPR1基因的功能提供方便.展开更多
Immune checkpoint inhibitors have a poor effect in treating ovarian cancer,and the specific mechanism is unknown.The purpose of this research was to investigate the impact of XPR1 on controlling autophagy in ovarian c...Immune checkpoint inhibitors have a poor effect in treating ovarian cancer,and the specific mechanism is unknown.The purpose of this research was to investigate the impact of XPR1 on controlling autophagy in ovarian cancer.The findings suggested an increase in XPR1 expression in ovarian cancer tissues.The elevated level of its expression was linked to the stage of ovarian cancer,as well as overall survival and progression-free survival.XPR1 enhanced the growth and spread of ovarian cancer while suppressing autophagy.Moreover,XPR1 suppressed autophagy flux by interacting with LAMP1 and the PI3K/Akt/mTOR pathway.XPR1 controlled the positioning and production of MHC-I molecules on the surfaces of ovarian cancer cells via autophagy.Silencing XPR1 combined with the autophagy inhibitor chloroquine significantly inhibited tumor growth in mouse ovarian cancer models.In conclusion,the findings indicate that XPR1 could serve as a promising target for the diagnosis and treatment of ovarian cancer.Combined autophagy inhibitors may improve the sensitivity of ovarian cancer immunotherapy.展开更多
基金supported by grants from the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical UniversitySenior Medical Talents Program of Chongqing for Young and Middle-agedChongqing Science and Technology Bureau Project (CSTB2024TIAD-KPX0038).
文摘Immune checkpoint inhibitors have a poor effect in treating ovarian cancer,and the specific mechanism is unknown.The purpose of this research was to investigate the impact of XPR1 on controlling autophagy in ovarian cancer.The findings suggested an increase in XPR1 expression in ovarian cancer tissues.The elevated level of its expression was linked to the stage of ovarian cancer,as well as overall survival and progression-free survival.XPR1 enhanced the growth and spread of ovarian cancer while suppressing autophagy.Moreover,XPR1 suppressed autophagy flux by interacting with LAMP1 and the PI3K/Akt/mTOR pathway.XPR1 controlled the positioning and production of MHC-I molecules on the surfaces of ovarian cancer cells via autophagy.Silencing XPR1 combined with the autophagy inhibitor chloroquine significantly inhibited tumor growth in mouse ovarian cancer models.In conclusion,the findings indicate that XPR1 could serve as a promising target for the diagnosis and treatment of ovarian cancer.Combined autophagy inhibitors may improve the sensitivity of ovarian cancer immunotherapy.
