Xiaoaiping(XAP)Injection demonstrates the anti-prostate cancer(PCa)effects,yet the underlying mechanism remains unclear.This study aims to investigate the impact of XAP on PCa and elucidate its mechanism of action.PCa...Xiaoaiping(XAP)Injection demonstrates the anti-prostate cancer(PCa)effects,yet the underlying mechanism remains unclear.This study aims to investigate the impact of XAP on PCa and elucidate its mechanism of action.PCa cell proliferation was evaluated using a cell counting kit-8(CCK-8)assay.Cell apoptosis was assessed through Hoechst staining and Western blotting assays.Proteomics technology was employed to identify key molecules and significant signaling pathways modulated by XAP in PCa cells.To further validate potential key genes and important pathways,a series of assays were conducted,including acridine orange(AO)staining,transmission electron microscopy,and immunofluorescence assays.The molecular mechanism of XAP against PCa in vivo was examined using a PC3 xenograft mouse model.Results demonstrated that XAP significantly inhibited cell proliferation in multiple PCa cell lines.In C4-2 and prostate cancer cell line-3(PC3)cells,XAP induced cellular apoptosis,evidenced by reduced B-cell lymphoma 2(Bcl-2)levels and elevated Bcl-2-associated X(Bax)levels.Proteomic,immunofluorescence,and quantitative reverse transcription-polymerase chain reaction(q RT-PCR)investigations revealed a strong correlation between forkhead box O3a(FoxO3a)autophagic degradation and the anti-PCa action of XAP.XAP hindered autophagy by reducing the expression levels of autophagy-related protein 5(Atg5)/autophagy-related protein 12(Atg12)and enhancing FoxO3a expression and nuclear translocation.Furthermore,XAP exhibited potent anti-PCa action in PC3 xenograft mice and triggered FoxO3a nuclear translocation in tumor tissue.These findings suggest that XAP induces PCa apoptosis via inhibition of FoxO3a autophagic degradation,potentially offering a novel perspective on XAP injection as an effective anticancer therapy for PCa.展开更多
Xiaoaiping injection is a single-prescription of Marsdenia tenacissima. One new compound, tenacigenoside L （1）, along with ten known compounds were isolated from the CHCl3-soluble fraction of Xiaoaiping injection ex...Xiaoaiping injection is a single-prescription of Marsdenia tenacissima. One new compound, tenacigenoside L （1）, along with ten known compounds were isolated from the CHCl3-soluble fraction of Xiaoaiping injection extract by silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. The new compound was characterized as 3-O-β-D-oleandropyranosyl- 17β-tenacigenin B. The ten known compounds were identified as tenacigenin B （2）, 17β-tenacigenin B （3）, marsdenoside F （4）, tenacissoside G （5）, tenacissoside I （6）, tenacissoside H （7）, marsdenoside D （8）, tenacigenin A （9）, marsdenoside I （10）, and tenacigenin C （11）. The structures of 11 compounds were elucidated on the basis of extensive analyses of spectroscopic data including MS, 1D NMR and 2D NMR, and comparison of their spectroscopic data with those reported in the literatures.展开更多
Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitth...Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitthe growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker andis closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression andXAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, weaimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the differentinvasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowedcomparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasisassociated with AFP and XAP treatment.Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in thecontrol group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantlydecreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize waspromoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAPinjection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases(MMPs).Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expressionof AFP;additionally, this inhibition of AFP is achieved by affecting MMPs.展开更多
Objective:To evaluate the clinical efficacy of Xiaoaiping injection(XAPI)combined with cisplatin plus gemcitabine(regimen of GP)for patients with non-small cell lung cancer(NSCLC).Methods:A literature search was condu...Objective:To evaluate the clinical efficacy of Xiaoaiping injection(XAPI)combined with cisplatin plus gemcitabine(regimen of GP)for patients with non-small cell lung cancer(NSCLC).Methods:A literature search was conducted for collecting the randomized controlled trials(RCTs)on NSCLC treated by Xiaoaiping injection and GP in the Cochrane Library,PubMed,Embase,Chinese National Knowledge Infrastructure(CNKI),China Biology Medicine(CBM),China Science and Technology Journal Database(VIP)and the Wanfang Database from inception to December,2018.The quality of the RCTs was evaluated by the Cochrane risk of bias assessment tool,and data analysis were performed with Review Manager 5.3.Results:A total of 7 randomized controlled trials with 534 patients were incorporated.The results showed that there is no statistical significance in total effective rate[OR=1.39,95%CI(0.97,1.98),P=0.07]and gastrointestinal reactions rate[OR=0.44,95%CI(0.16,1.23),P=0.12]between GP alone and XAPI combined with GP.In comparison with GP alone,the XAPI combined with GP was associated with the lower effects on the decrease rate of hemoglobin[OR=0.49,95%CI(0.26,0.92),P=0.03],leukocyte[OR=0.40,95%CI(0.21,0.74),P=0.004]and platelet[OR=0.43,95%CI(0.22,0.87),P=0.02].However,performance status[OR=3.78,95%CI(2.24,6.38),P<0.0001]of patients in XAPI plus GP group is better than GP alone group.Conclusion:The combination of XAPI and GP has certain curative effect for patients with NSCLC compared with only receiving GP.However,more well-designedand multicenter RCTs should be performed to verify this result because of the quality of enrolled RCTs.展开更多
Objective:To study the effect of Xiaoaiping combined with intravenous chemotherapy on tumor marker molecule content and cell viability molecule expression in patients with advanced gastric cancer. Methods:94 patients ...Objective:To study the effect of Xiaoaiping combined with intravenous chemotherapy on tumor marker molecule content and cell viability molecule expression in patients with advanced gastric cancer. Methods:94 patients diagnosed with advanced gastric cancer in our hospital between May 2013 and March 2016 were selected and randomly divided into Xiaoaiping group and XELOX group who received Xiaoaiping combined with XELOX therapy and XELOX therapy alone respectively. After four cycles of treatment, serum was collected to detect tumor marker levels, and gastric cancer tissue was collected to detect the expression of invasion-, proliferation-and apoptosis-related molecules. Results:After four cycles of treatment, serum carcinoembryonic antigen (CEA), carbohydrate antigen (CA199), thymidine kinase (TK)-1, Pentraxin-3 and human epididymis protein 4 (HE4) levels of Xiaoaiping group were significantly lower than those of XELOX group (P<0.05);ALDH1, CXCR4, CatE, CatS, CyclinD1, CDK4, CDC25B, Bcl-2 and Survivin expression in gastric cancer tissue of Xiaoaiping group were significantly lower than those of XELOX group (P<0.05) while Caspase-3, Caspase-9, PTEN and Beclin-1 expression were significantly higher than those of XELOX group (P<0.05). Conclusions:Xiaoaiping combined with intravenous chemotherapy for advanced gastric cancer can more effectively kill cancer cells and inhibit cancer cell proliferation and invasion.展开更多
Objective To observe the therapeutic effect of the combination of Xiaoaiping injection and chemotherapy on advanced esophageal cancer and coagulation function.Methods 100 patients with advanced esophageal carcer were ...Objective To observe the therapeutic effect of the combination of Xiaoaiping injection and chemotherapy on advanced esophageal cancer and coagulation function.Methods 100 patients with advanced esophageal carcer were randomly divided into control group and observation group,and each group had 50 cases.The control group was treated with TP chemotherapy,and the observation group,on the basis of the control group’s treatment,was treated with the Xiaoaiping injection,and treatment effects,Karnofsky,adverse drug reactions and INR changes before and after the treatment of the two groups were observed.Results After 2 periods of treatment,the local control rate of solid tumor,Karnofsky score,and stability in the observation group were significantly higher than those in the control group(p<0.05);and the plasma prothrombin time(PT),activated partial coagulation activity time(APTT)and thrombin time(TT)were significantly lower in the observation group than in the control group(p<0.05),and Fibrinogen(FIB)was significantly higher than the control group(p<0.05);and there was no statistically significant difference in the incidence of adverse reactions between the two groups(p>0.05).Conclusion:The therapeutic effect of the combination of Xiaoaiping Injection and chemotherapy on advanced esophageal cancer is obvious,and it can effectively improve the coagulation function,improve the quality of life,and be safe and reliable,so it's worth popularizing and application.展开更多
Objective:To study the effect of preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy on malignant biological behaviors of breast cancer cells.Methods:The patients with breast cancer who received neoadju...Objective:To study the effect of preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy on malignant biological behaviors of breast cancer cells.Methods:The patients with breast cancer who received neoadjuvant chemotherapy in Renshou County People's Hospital between June 2014 and December 2017 were chosen as the research subjects and randomly divided into the combined group who accepted preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy and the control group who accepted TEC neoadjuvant chemotherapy. After surgical resection, the breast cancer lesion was taken to determine the mRNA expression of proliferation genes and invasion genes as well as the protein levels of angiogenesis molecules.Results: After surgical resection, USP39, CyclinD1, c-myc, Sema4D, CatB, ADAM17 and uPA mRNA expression as well as VEGF, Ang-2 and bFGF protein levels in breast cancer lesions of combined group were significantly lower than those of control group whereas TCEAL17, MFN2, TIMP1 and E-cadherin mRNA expression as well as MMRN2 and PEDF protein levels were significantly higher than those of control group.Conclusion:Xiaoaiping combined with TEC neoadjuvant chemotherapy before breast cancer surgery can be more effective than TEC neoadjuvant chemotherapy alone to inhibit the proliferation, invasion and angiogenesis of cancer cells.展开更多
Objective： Investigation of the effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells. Methods： Selecting the HepG2 cells in the logarithmic growth phase and assigning them to...Objective： Investigation of the effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells. Methods： Selecting the HepG2 cells in the logarithmic growth phase and assigning them to Xiaoaiping injection （XAP） group and control group. The two groups were treated with 75 mg/mL XAP or the same dose of normal saline. After 72 h of treatment, real-time PCR was used to detect the expression of circadian clock genes in HepG2 cells and Western Blot technology was used to detect the expression of related proteins. Results： The mRNA expression levels of PER1, NPAS2, NR1D1, and DEC1 in the XAP group was significantly higher than that in the control group （P〈 0.05）, while the mRNA expression levels of PER3, BMAL1, DEC2, and RORA were significantly lower in the XAP group than in the control group （P 〈 0.05）, and there was no significant difference between the mRNA expression levels of PER2, CRY1, CRY2, and TIM. Of course, the proteins＇ expression levels of the genes we had detected such as PERle3, CRYI-2, CLOCK, BMAL1 by Western Blot were consistent with the real-time PCR results above. Conclusion： XAP affects the expression of circadian clock genes in HepG2 cells.展开更多
Objective: To investigate the effect of Xiaoaiping combined with neoadjuvant chemotherapy on the pro-proliferation molecule expression and immune function in patients with breast cancer. Methods: A total of 98 patient...Objective: To investigate the effect of Xiaoaiping combined with neoadjuvant chemotherapy on the pro-proliferation molecule expression and immune function in patients with breast cancer. Methods: A total of 98 patients with primary breast cancer who were diagnosed and treated in the hospital between December 2015 and February 2017 were collected and divided into control group and Xiaoaiping group by random number table, each with 49 cases. Control group received neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy, and Xiaoaiping group received Xiaoaiping + neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy. The differences in pro-proliferation gene expression in intraoperative breast cancer tissue as well as the differences in serum levels of Th1/Th2 cytokines and Th17/Treg cytokines before chemotherapy started (T0) and 1 week after neoadjuvant chemotherapy ended (T1) were compared between the two groups of patients. Results: MTA2, NRP-1, PKM2, TM4SF1 and ZIC1 mRNA expression in breast cancer tissue of Xiaoaiping group were lower than those of control group. At T1, serum IFN-γ and TNF-α levels of Xiaoaiping group were higher than those of control group whereas IL-4, IL-10, IL-17, IL-22, IL-35 and TGF-β levels were lower than those of control group. Conclusion: Xiaoaiping combined with neoadjuvant chemotherapy can effectively improve the curative effect of preoperative chemotherapy, and also significantly inhibit the proliferation activity of breast cancer cells and balance the immune function of the body.展开更多
Objective:To study the effect of Xiaoaiping combined with 5-fluorouracil treatment on liver cancer cell growth in vitro and oncogene expression.Methods: Liver cancer cell lines SMMC-7721 were cultured and treated with...Objective:To study the effect of Xiaoaiping combined with 5-fluorouracil treatment on liver cancer cell growth in vitro and oncogene expression.Methods: Liver cancer cell lines SMMC-7721 were cultured and treated with different doses of Xiaoaiping (10, 20 and 40 μL/mL) and 5-fluorouracil (10, 20 and 40 μmol/mL). 12 h, 24 h and 48 h after treatment, the CCK-8 kits were used to determine the cell viability;24 h after treatment, enzyme-linked immunosorbent assay kits were used to determine proliferation and apoptosis gene expression.Results: 12 h, 24 h and 48 h after treatment, different doses of Xiaoaiping and 5-fluorouracil could all reduce cell proliferation activity in dose-dependent manner and the cell proliferation activity of 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly lower than those of 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group;24 h after treatment, P16INK4, P57kip2, Caspase-3 and PDCD5 protein expression in 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group, 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group were significantly higher than those in control group while LETM1, SIRT6 and UHRF1 protein expression were significantly lower than those in control group, and P16INK4, P57kip2, Caspase-3 and PDCD5 protein expression in 40μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly higher than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group while LETM1, SIRT6 and UHRF1 protein expression were significantly lower than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group.Conclusion: Xiaoaiping combined with 5-fluorouracil has inhibitory effect on liver cancer cell proliferation, and can also increase the expression of apoptosis genes and reduce the expression of proliferation genes.展开更多
OBJECTIVE: To investigate the clinical efficacy and safety of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small celllung cancer(NCSLC) comp...OBJECTIVE: To investigate the clinical efficacy and safety of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small celllung cancer(NCSLC) compared with chemotherapy alone.METHODS: Databases including Chinese National Knowledge Infrastructure, China Biology Medicine Disc, Wanfang, and MEDLINE were searched until April 1, 2014. Two assessors independently reviewed each trial. The primary outcome was the effective rate(ER) of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy. The secondary outcomes included quality of life improvement rate(QOLIR) and adverse reactions. Statistical calculations were performed by using Cochrane Collaboration Review Manager 5.2.RESULTS: A total of 888 patients from 15 studies,13 randomized controlled trials(RCT) and two controlled clinical trials, were included. Compared with chemotherapy alone, Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly improved ER [Risk ratio(RR) =1.32, 95% CI,(1.14, 1.54)](based on 15 studies) and QOLIR [RR = 2.04, 95% CI,(1.69, 2.47)](based on 13studies). Compared with chemotherapy alone,Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly inhibited chemotherapy-induced white blood cell decline [RR = 0.79, 95% CI,(0.70, 0.90)(based on 10 studies), chemotherapy-induced platelet decline[RR = 0.77, 95% CI,(0.60, 0.98)](based on 8 studies),and significantly alleviated nausea and vomiting(NV) [RR = 0.83, 95% CI,(0.71, 0.97)](based on 7studies). There was no significant difference in hemoglobin decline between the two therapies [RR =0.88, 95% CI,(0.70, 1.09)](based on 6 studies).CONCLUSION: This Meta-analysis suggests that Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy may be more efficacious in the treatment of advanced NSCLC than chemotherapy alone. This effect includes enhancing ER and QOLIR, and weakening chemotherapy toxicity. However, large-scale RCTs are required to further investigate the short- and long-term effects of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract.展开更多
基金supported by Guangxi Natural Science Foundation(No.2023GXNSFDA026047)Guangxi Science and Technology Project(No.FANGKE ZY20221502)+1 种基金the National Natural Science Foundation of China(No.82160948)the Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University。
文摘Xiaoaiping(XAP)Injection demonstrates the anti-prostate cancer(PCa)effects,yet the underlying mechanism remains unclear.This study aims to investigate the impact of XAP on PCa and elucidate its mechanism of action.PCa cell proliferation was evaluated using a cell counting kit-8(CCK-8)assay.Cell apoptosis was assessed through Hoechst staining and Western blotting assays.Proteomics technology was employed to identify key molecules and significant signaling pathways modulated by XAP in PCa cells.To further validate potential key genes and important pathways,a series of assays were conducted,including acridine orange(AO)staining,transmission electron microscopy,and immunofluorescence assays.The molecular mechanism of XAP against PCa in vivo was examined using a PC3 xenograft mouse model.Results demonstrated that XAP significantly inhibited cell proliferation in multiple PCa cell lines.In C4-2 and prostate cancer cell line-3(PC3)cells,XAP induced cellular apoptosis,evidenced by reduced B-cell lymphoma 2(Bcl-2)levels and elevated Bcl-2-associated X(Bax)levels.Proteomic,immunofluorescence,and quantitative reverse transcription-polymerase chain reaction(q RT-PCR)investigations revealed a strong correlation between forkhead box O3a(FoxO3a)autophagic degradation and the anti-PCa action of XAP.XAP hindered autophagy by reducing the expression levels of autophagy-related protein 5(Atg5)/autophagy-related protein 12(Atg12)and enhancing FoxO3a expression and nuclear translocation.Furthermore,XAP exhibited potent anti-PCa action in PC3 xenograft mice and triggered FoxO3a nuclear translocation in tumor tissue.These findings suggest that XAP induces PCa apoptosis via inhibition of FoxO3a autophagic degradation,potentially offering a novel perspective on XAP injection as an effective anticancer therapy for PCa.
文摘Xiaoaiping injection is a single-prescription of Marsdenia tenacissima. One new compound, tenacigenoside L （1）, along with ten known compounds were isolated from the CHCl3-soluble fraction of Xiaoaiping injection extract by silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. The new compound was characterized as 3-O-β-D-oleandropyranosyl- 17β-tenacigenin B. The ten known compounds were identified as tenacigenin B （2）, 17β-tenacigenin B （3）, marsdenoside F （4）, tenacissoside G （5）, tenacissoside I （6）, tenacissoside H （7）, marsdenoside D （8）, tenacigenin A （9）, marsdenoside I （10）, and tenacigenin C （11）. The structures of 11 compounds were elucidated on the basis of extensive analyses of spectroscopic data including MS, 1D NMR and 2D NMR, and comparison of their spectroscopic data with those reported in the literatures.
文摘Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitthe growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker andis closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression andXAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, weaimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the differentinvasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowedcomparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasisassociated with AFP and XAP treatment.Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in thecontrol group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantlydecreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize waspromoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAPinjection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases(MMPs).Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expressionof AFP;additionally, this inhibition of AFP is achieved by affecting MMPs.
文摘Objective:To evaluate the clinical efficacy of Xiaoaiping injection(XAPI)combined with cisplatin plus gemcitabine(regimen of GP)for patients with non-small cell lung cancer(NSCLC).Methods:A literature search was conducted for collecting the randomized controlled trials(RCTs)on NSCLC treated by Xiaoaiping injection and GP in the Cochrane Library,PubMed,Embase,Chinese National Knowledge Infrastructure(CNKI),China Biology Medicine(CBM),China Science and Technology Journal Database(VIP)and the Wanfang Database from inception to December,2018.The quality of the RCTs was evaluated by the Cochrane risk of bias assessment tool,and data analysis were performed with Review Manager 5.3.Results:A total of 7 randomized controlled trials with 534 patients were incorporated.The results showed that there is no statistical significance in total effective rate[OR=1.39,95%CI(0.97,1.98),P=0.07]and gastrointestinal reactions rate[OR=0.44,95%CI(0.16,1.23),P=0.12]between GP alone and XAPI combined with GP.In comparison with GP alone,the XAPI combined with GP was associated with the lower effects on the decrease rate of hemoglobin[OR=0.49,95%CI(0.26,0.92),P=0.03],leukocyte[OR=0.40,95%CI(0.21,0.74),P=0.004]and platelet[OR=0.43,95%CI(0.22,0.87),P=0.02].However,performance status[OR=3.78,95%CI(2.24,6.38),P<0.0001]of patients in XAPI plus GP group is better than GP alone group.Conclusion:The combination of XAPI and GP has certain curative effect for patients with NSCLC compared with only receiving GP.However,more well-designedand multicenter RCTs should be performed to verify this result because of the quality of enrolled RCTs.
文摘Objective:To study the effect of Xiaoaiping combined with intravenous chemotherapy on tumor marker molecule content and cell viability molecule expression in patients with advanced gastric cancer. Methods:94 patients diagnosed with advanced gastric cancer in our hospital between May 2013 and March 2016 were selected and randomly divided into Xiaoaiping group and XELOX group who received Xiaoaiping combined with XELOX therapy and XELOX therapy alone respectively. After four cycles of treatment, serum was collected to detect tumor marker levels, and gastric cancer tissue was collected to detect the expression of invasion-, proliferation-and apoptosis-related molecules. Results:After four cycles of treatment, serum carcinoembryonic antigen (CEA), carbohydrate antigen (CA199), thymidine kinase (TK)-1, Pentraxin-3 and human epididymis protein 4 (HE4) levels of Xiaoaiping group were significantly lower than those of XELOX group (P<0.05);ALDH1, CXCR4, CatE, CatS, CyclinD1, CDK4, CDC25B, Bcl-2 and Survivin expression in gastric cancer tissue of Xiaoaiping group were significantly lower than those of XELOX group (P<0.05) while Caspase-3, Caspase-9, PTEN and Beclin-1 expression were significantly higher than those of XELOX group (P<0.05). Conclusions:Xiaoaiping combined with intravenous chemotherapy for advanced gastric cancer can more effectively kill cancer cells and inhibit cancer cell proliferation and invasion.
文摘Objective To observe the therapeutic effect of the combination of Xiaoaiping injection and chemotherapy on advanced esophageal cancer and coagulation function.Methods 100 patients with advanced esophageal carcer were randomly divided into control group and observation group,and each group had 50 cases.The control group was treated with TP chemotherapy,and the observation group,on the basis of the control group’s treatment,was treated with the Xiaoaiping injection,and treatment effects,Karnofsky,adverse drug reactions and INR changes before and after the treatment of the two groups were observed.Results After 2 periods of treatment,the local control rate of solid tumor,Karnofsky score,and stability in the observation group were significantly higher than those in the control group(p<0.05);and the plasma prothrombin time(PT),activated partial coagulation activity time(APTT)and thrombin time(TT)were significantly lower in the observation group than in the control group(p<0.05),and Fibrinogen(FIB)was significantly higher than the control group(p<0.05);and there was no statistically significant difference in the incidence of adverse reactions between the two groups(p>0.05).Conclusion:The therapeutic effect of the combination of Xiaoaiping Injection and chemotherapy on advanced esophageal cancer is obvious,and it can effectively improve the coagulation function,improve the quality of life,and be safe and reliable,so it's worth popularizing and application.
文摘Objective:To study the effect of preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy on malignant biological behaviors of breast cancer cells.Methods:The patients with breast cancer who received neoadjuvant chemotherapy in Renshou County People's Hospital between June 2014 and December 2017 were chosen as the research subjects and randomly divided into the combined group who accepted preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy and the control group who accepted TEC neoadjuvant chemotherapy. After surgical resection, the breast cancer lesion was taken to determine the mRNA expression of proliferation genes and invasion genes as well as the protein levels of angiogenesis molecules.Results: After surgical resection, USP39, CyclinD1, c-myc, Sema4D, CatB, ADAM17 and uPA mRNA expression as well as VEGF, Ang-2 and bFGF protein levels in breast cancer lesions of combined group were significantly lower than those of control group whereas TCEAL17, MFN2, TIMP1 and E-cadherin mRNA expression as well as MMRN2 and PEDF protein levels were significantly higher than those of control group.Conclusion:Xiaoaiping combined with TEC neoadjuvant chemotherapy before breast cancer surgery can be more effective than TEC neoadjuvant chemotherapy alone to inhibit the proliferation, invasion and angiogenesis of cancer cells.
文摘Objective： Investigation of the effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells. Methods： Selecting the HepG2 cells in the logarithmic growth phase and assigning them to Xiaoaiping injection （XAP） group and control group. The two groups were treated with 75 mg/mL XAP or the same dose of normal saline. After 72 h of treatment, real-time PCR was used to detect the expression of circadian clock genes in HepG2 cells and Western Blot technology was used to detect the expression of related proteins. Results： The mRNA expression levels of PER1, NPAS2, NR1D1, and DEC1 in the XAP group was significantly higher than that in the control group （P〈 0.05）, while the mRNA expression levels of PER3, BMAL1, DEC2, and RORA were significantly lower in the XAP group than in the control group （P 〈 0.05）, and there was no significant difference between the mRNA expression levels of PER2, CRY1, CRY2, and TIM. Of course, the proteins＇ expression levels of the genes we had detected such as PERle3, CRYI-2, CLOCK, BMAL1 by Western Blot were consistent with the real-time PCR results above. Conclusion： XAP affects the expression of circadian clock genes in HepG2 cells.
文摘Objective: To investigate the effect of Xiaoaiping combined with neoadjuvant chemotherapy on the pro-proliferation molecule expression and immune function in patients with breast cancer. Methods: A total of 98 patients with primary breast cancer who were diagnosed and treated in the hospital between December 2015 and February 2017 were collected and divided into control group and Xiaoaiping group by random number table, each with 49 cases. Control group received neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy, and Xiaoaiping group received Xiaoaiping + neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy. The differences in pro-proliferation gene expression in intraoperative breast cancer tissue as well as the differences in serum levels of Th1/Th2 cytokines and Th17/Treg cytokines before chemotherapy started (T0) and 1 week after neoadjuvant chemotherapy ended (T1) were compared between the two groups of patients. Results: MTA2, NRP-1, PKM2, TM4SF1 and ZIC1 mRNA expression in breast cancer tissue of Xiaoaiping group were lower than those of control group. At T1, serum IFN-γ and TNF-α levels of Xiaoaiping group were higher than those of control group whereas IL-4, IL-10, IL-17, IL-22, IL-35 and TGF-β levels were lower than those of control group. Conclusion: Xiaoaiping combined with neoadjuvant chemotherapy can effectively improve the curative effect of preoperative chemotherapy, and also significantly inhibit the proliferation activity of breast cancer cells and balance the immune function of the body.
文摘Objective:To study the effect of Xiaoaiping combined with 5-fluorouracil treatment on liver cancer cell growth in vitro and oncogene expression.Methods: Liver cancer cell lines SMMC-7721 were cultured and treated with different doses of Xiaoaiping (10, 20 and 40 μL/mL) and 5-fluorouracil (10, 20 and 40 μmol/mL). 12 h, 24 h and 48 h after treatment, the CCK-8 kits were used to determine the cell viability;24 h after treatment, enzyme-linked immunosorbent assay kits were used to determine proliferation and apoptosis gene expression.Results: 12 h, 24 h and 48 h after treatment, different doses of Xiaoaiping and 5-fluorouracil could all reduce cell proliferation activity in dose-dependent manner and the cell proliferation activity of 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly lower than those of 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group;24 h after treatment, P16INK4, P57kip2, Caspase-3 and PDCD5 protein expression in 40 μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group, 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group were significantly higher than those in control group while LETM1, SIRT6 and UHRF1 protein expression were significantly lower than those in control group, and P16INK4, P57kip2, Caspase-3 and PDCD5 protein expression in 40μL/mL Xiaoaiping + 40 μmol/mL 5-Fu group were significantly higher than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group while LETM1, SIRT6 and UHRF1 protein expression were significantly lower than those in 40 μL/mL Xiaoaiping group and 40 μmol/mL 5-Fu group.Conclusion: Xiaoaiping combined with 5-fluorouracil has inhibitory effect on liver cancer cell proliferation, and can also increase the expression of apoptosis genes and reduce the expression of proliferation genes.
基金the National Natural Science Foundation of China(Mechanism of Acupuncture in Treating Insulin Resistance Obese Through Modulating Hypothalamic SIRT1/Fox O1,No.81473787,and No.81574065)
文摘OBJECTIVE: To investigate the clinical efficacy and safety of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small celllung cancer(NCSLC) compared with chemotherapy alone.METHODS: Databases including Chinese National Knowledge Infrastructure, China Biology Medicine Disc, Wanfang, and MEDLINE were searched until April 1, 2014. Two assessors independently reviewed each trial. The primary outcome was the effective rate(ER) of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy. The secondary outcomes included quality of life improvement rate(QOLIR) and adverse reactions. Statistical calculations were performed by using Cochrane Collaboration Review Manager 5.2.RESULTS: A total of 888 patients from 15 studies,13 randomized controlled trials(RCT) and two controlled clinical trials, were included. Compared with chemotherapy alone, Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly improved ER [Risk ratio(RR) =1.32, 95% CI,(1.14, 1.54)](based on 15 studies) and QOLIR [RR = 2.04, 95% CI,(1.69, 2.47)](based on 13studies). Compared with chemotherapy alone,Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly inhibited chemotherapy-induced white blood cell decline [RR = 0.79, 95% CI,(0.70, 0.90)(based on 10 studies), chemotherapy-induced platelet decline[RR = 0.77, 95% CI,(0.60, 0.98)](based on 8 studies),and significantly alleviated nausea and vomiting(NV) [RR = 0.83, 95% CI,(0.71, 0.97)](based on 7studies). There was no significant difference in hemoglobin decline between the two therapies [RR =0.88, 95% CI,(0.70, 1.09)](based on 6 studies).CONCLUSION: This Meta-analysis suggests that Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy may be more efficacious in the treatment of advanced NSCLC than chemotherapy alone. This effect includes enhancing ER and QOLIR, and weakening chemotherapy toxicity. However, large-scale RCTs are required to further investigate the short- and long-term effects of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract.
