Patulin(PAT)is a neurotoxic fungal metabolite that commonly contaminates fruits and their derivatives,causing substantial economic loss and serious harm to human health.In this study,we screened a PAT-degrading yeast ...Patulin(PAT)is a neurotoxic fungal metabolite that commonly contaminates fruits and their derivatives,causing substantial economic loss and serious harm to human health.In this study,we screened a PAT-degrading yeast strain,Geotrichum candidum XG1,among 12 probiotic strains.It efficiently degraded 96.80%of PAT(5μg/mL)within 48 h in potato dextrose broth and completely degraded PAT in fresh apple juice.G.candidum XG1 degraded PAT mainly by secreting intracellular enzymes,which were induced by PAT,and the degradation rate increased with increasing induction time.Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry identified ascladiol as a PAT degradation product that disappeared within 96 h.Notably,both G.candidum XG1 and its intracellular enzymes affected the quality of apple juice,and G.candidum XG1 enhanced the flavor of apple juice.Overall,these results highlight the potential of G.candidum XG1 to remove PAT from apple juice.展开更多
基金supported by the National Key R&D Program of China(2021YFE0116500)Graduate Innovation Project of Chongqing(CYB22132).
文摘Patulin(PAT)is a neurotoxic fungal metabolite that commonly contaminates fruits and their derivatives,causing substantial economic loss and serious harm to human health.In this study,we screened a PAT-degrading yeast strain,Geotrichum candidum XG1,among 12 probiotic strains.It efficiently degraded 96.80%of PAT(5μg/mL)within 48 h in potato dextrose broth and completely degraded PAT in fresh apple juice.G.candidum XG1 degraded PAT mainly by secreting intracellular enzymes,which were induced by PAT,and the degradation rate increased with increasing induction time.Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry identified ascladiol as a PAT degradation product that disappeared within 96 h.Notably,both G.candidum XG1 and its intracellular enzymes affected the quality of apple juice,and G.candidum XG1 enhanced the flavor of apple juice.Overall,these results highlight the potential of G.candidum XG1 to remove PAT from apple juice.
