目的探究血清分泌型卷曲相关蛋白5(secreted frizzled related protein 5,SFRP-5)、C-X-C基序趋化因子配体16(C-X-C motif chemokine ligand 16,CXCL16)对心肌梗死患者经皮冠状动脉介入(percutaneous coronary intervention,PCI)治疗后...目的探究血清分泌型卷曲相关蛋白5(secreted frizzled related protein 5,SFRP-5)、C-X-C基序趋化因子配体16(C-X-C motif chemokine ligand 16,CXCL16)对心肌梗死患者经皮冠状动脉介入(percutaneous coronary intervention,PCI)治疗后心力衰竭(heart failure,HF)发生的预测价值。方法选取2022年6月至2024年1月于保定市第二中心医院实施PCI治疗的心肌梗死患者152例为研究对象。术后随访1年,根据患者是否发生HF分为HF组和(n=47)非HF组(n=105)。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测PCI治疗前患者血清SFRP-5、CXCL16的浓度;采用多因素Logistic回归法分析影响心肌梗死患者PCI治疗后HF发生的因素;绘制受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SFRP-5、CXCL16浓度对心肌梗死患者PCI治疗后HF发生的预测价值。结果与非HF组患者比较,HF组患者性别、体质量指数(body mass index,BMI)、吸烟史、原发性高血压(高血压)史、糖尿病史、收缩压、舒张压及血肌酐(serum creatinine,Scr)、空腹血糖、总胆固醇浓度比较,差异无统计学意义(P>0.05);血清SFRP-5浓度显著降低,年龄及纤维蛋白原、CXCL16浓度浓度显著升高,差异有统计学意义(P<0.05)。年龄(OR=1.712)、纤维蛋白原(OR=2.240)、CXCL16浓度升高(OR=3.948),SFRP-5浓度降低(OR=0.284)均是影响心肌梗死患者PCI治疗后HF发生的危险因素(P<0.05)。血清SFRP-5、CXCL16浓度单独预测心肌梗死患者PCI治疗后HF发生的曲线下面积(area under the curve,AUC)分别为0.698(95%CI:0.619~0.770)、0.801(95%CI:0.729~0.861),二者联合预测的AUC为0.952(95%CI:0.905~0.980),灵敏度为91.49%,特异度为91.43%。联合预测价值显著高于单一指标预测(Z_(三者联合-SFRP-5)=5.839、Z_(三者联合-CXCL16)=3.564,P<0.05)。结论血清SFRP-5、CXCL16浓度对心肌梗死患者PCI治疗后HF发生具有一定的预测价值,且二者联合的预测价值更高。展开更多
Background: Recent studies indicate that C-X-C motif chemokine receptor 4(CXCR4) and its ligand, C-X-C motif chemokine ligand 12(CXCL12), stimulate expression of the cell cycle regulatory protein Cyclin D1 in neurofib...Background: Recent studies indicate that C-X-C motif chemokine receptor 4(CXCR4) and its ligand, C-X-C motif chemokine ligand 12(CXCL12), stimulate expression of the cell cycle regulatory protein Cyclin D1 in neurofibromatosis 1-associated malignant peripheral nerve sheath tumor(MPNST) cells and promote their proliferation. In this study, we measured the expression of CXCR4, CXCL12, and Cyclin D1 proteins in sporadic MPNST tissues from Chinese patients and investigated their prognostic values.Methods: CXCR4, CXCL12, and Cyclin D1 protein expression in samples from 58 Chinese patients with sporadic MPNST was assessed with immunohistochemical staining.Their prognostic values were evaluated with Kaplan-Meier analysis and a log-rank test. Multivariate Cox regression analysis was used to identify independent prognostic factors.Results: High expression of CXCR4, CXCL12, and Cyclin D1 was observed in 19(32.8%), 32(55.2%), and 16(27.6%)samples, respectively. CXCR4 expression was positively correlated with CXCL12 expression(r = 0.334, P = 0.010) and Cyclin D1 expression(r = 0.309, P = 0.018). Patients with high CXCR4 expression showed longer overall survival than those with low CXCR4 expression(χ~2 = 4.642, P = 0.031).Conclusion: High CXCR4 expression may define a specific subtype of sporadic MPNST with favorable prognosis.展开更多
文摘目的探究血清分泌型卷曲相关蛋白5(secreted frizzled related protein 5,SFRP-5)、C-X-C基序趋化因子配体16(C-X-C motif chemokine ligand 16,CXCL16)对心肌梗死患者经皮冠状动脉介入(percutaneous coronary intervention,PCI)治疗后心力衰竭(heart failure,HF)发生的预测价值。方法选取2022年6月至2024年1月于保定市第二中心医院实施PCI治疗的心肌梗死患者152例为研究对象。术后随访1年,根据患者是否发生HF分为HF组和(n=47)非HF组(n=105)。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测PCI治疗前患者血清SFRP-5、CXCL16的浓度;采用多因素Logistic回归法分析影响心肌梗死患者PCI治疗后HF发生的因素;绘制受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SFRP-5、CXCL16浓度对心肌梗死患者PCI治疗后HF发生的预测价值。结果与非HF组患者比较,HF组患者性别、体质量指数(body mass index,BMI)、吸烟史、原发性高血压(高血压)史、糖尿病史、收缩压、舒张压及血肌酐(serum creatinine,Scr)、空腹血糖、总胆固醇浓度比较,差异无统计学意义(P>0.05);血清SFRP-5浓度显著降低,年龄及纤维蛋白原、CXCL16浓度浓度显著升高,差异有统计学意义(P<0.05)。年龄(OR=1.712)、纤维蛋白原(OR=2.240)、CXCL16浓度升高(OR=3.948),SFRP-5浓度降低(OR=0.284)均是影响心肌梗死患者PCI治疗后HF发生的危险因素(P<0.05)。血清SFRP-5、CXCL16浓度单独预测心肌梗死患者PCI治疗后HF发生的曲线下面积(area under the curve,AUC)分别为0.698(95%CI:0.619~0.770)、0.801(95%CI:0.729~0.861),二者联合预测的AUC为0.952(95%CI:0.905~0.980),灵敏度为91.49%,特异度为91.43%。联合预测价值显著高于单一指标预测(Z_(三者联合-SFRP-5)=5.839、Z_(三者联合-CXCL16)=3.564,P<0.05)。结论血清SFRP-5、CXCL16浓度对心肌梗死患者PCI治疗后HF发生具有一定的预测价值,且二者联合的预测价值更高。
基金supported by the National Nature Science Foundation of China(81372872 to Jilong Yang,81672650 to Ze Zhu,and 81402215 to Xiaoling Du)funds from the University Cancer Foundation via the Sister Institution Network Fund(to Jilong Yang)
文摘Background: Recent studies indicate that C-X-C motif chemokine receptor 4(CXCR4) and its ligand, C-X-C motif chemokine ligand 12(CXCL12), stimulate expression of the cell cycle regulatory protein Cyclin D1 in neurofibromatosis 1-associated malignant peripheral nerve sheath tumor(MPNST) cells and promote their proliferation. In this study, we measured the expression of CXCR4, CXCL12, and Cyclin D1 proteins in sporadic MPNST tissues from Chinese patients and investigated their prognostic values.Methods: CXCR4, CXCL12, and Cyclin D1 protein expression in samples from 58 Chinese patients with sporadic MPNST was assessed with immunohistochemical staining.Their prognostic values were evaluated with Kaplan-Meier analysis and a log-rank test. Multivariate Cox regression analysis was used to identify independent prognostic factors.Results: High expression of CXCR4, CXCL12, and Cyclin D1 was observed in 19(32.8%), 32(55.2%), and 16(27.6%)samples, respectively. CXCR4 expression was positively correlated with CXCL12 expression(r = 0.334, P = 0.010) and Cyclin D1 expression(r = 0.309, P = 0.018). Patients with high CXCR4 expression showed longer overall survival than those with low CXCR4 expression(χ~2 = 4.642, P = 0.031).Conclusion: High CXCR4 expression may define a specific subtype of sporadic MPNST with favorable prognosis.
