BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of li...BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of life of UC patients and may lead to deterioration of the patient’s condition.UC drugs combined with anti-anxiety and antidepression drugs can alleviate symptoms of both depression and UC.Brain-derived neurotrophic factor(BDNF)precursor(proBDNF)/p75 neurotrophin receptor(p75NTR)/sortilin and BDNF/tropomyosin receptor kinase B(TrkB)signalling balance is essential for maintaining brain homeostasis and preventing the development of depressive behaviours.AIM To explore the mechanism by which Wuling powder regulates the proBDNF/p75NTR/sortilin and BDNF/TrkB pathways in the treatment of UC with depre-ssion.METHODS Depression was established in C57BL/6J mice via chronic restraint stress,and the UC model was induced with dextran sodium sulfate(DSS).In the treatment stage,mesalazine(MS)was the basic treatment,Wuling powder was the experimental treatment,and fluoxetine was the positive control drug for treating depression.Changes in intestinal mucosal inflammation,behaviour,and the proBDNFp75NTR/sortilin and BDNF/TrkB pathways were evaluated.RESULTS In the depression groups,Wuling powder decreased the immobility time,increased the distance travelled in the central zone and the total distance travelled,and restored balance in the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways.In the DSS and chronic restraint stress+DSS groups,immobility time increased,distance travelled in the central zone and total distance travelled decreased,activity of the proBDNF/p75NTR/sortilin pathway was upregulated,and activity of the BDNF/TrkB pathway was downregulated,indicating that mice with UC often have comorbid depression.Compared with those of MS alone,Wuling powder combined with MS further decreased the colon histopathological scores and the expression levels of tumor necrosis factor-alpha and interleukin-6 mRNAs.CONCLUSION This study confirmed that Wuling powder may play an antidepressant role by regulating the balance of the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways and further relieve intestinal inflammation in UC.展开更多
[Objectives]To explore the action mechanism of Wuling Decoction(the combination of Wumei Pill and Wuling Powder)in treating Sjögren's syndrome(SS)based on network pharmacology and molecular docking techniques...[Objectives]To explore the action mechanism of Wuling Decoction(the combination of Wumei Pill and Wuling Powder)in treating Sjögren's syndrome(SS)based on network pharmacology and molecular docking techniques.[Methods]The active components and their targets of Wuling Decoction were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The targets related to SS were obtained from the GeneCards database.The intersection targets between Wuling Decoction and SS were identified using the Venny platform.The"Chinese medicinal-active component-common target"network was constructed using Cytoscape.The potential targets were improted into STRING to establish the protein-protein interaction(PPI)network,and the topological parameters were evaluated using Cytoscape to determine the key targets.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed using the DAVID database.Molecular docking of core components and key targets was conducted using the CB-Dock2 online tool.[Results]Wuling Decoction contained 121 active components of Chinese medicinals and 190 related targets.There were 1447 SS-related targets obtained from GeneCards databases.50 potential targets of Wuling decoction for SS were obtained.Network topology analysis revealed that quercetin,stigmasterol,andβ-sitosterol were core components.PPI network analysis indicated that TNF,IL-6,and IL-1βwere key targets.These targets were primarily involved in biological processes such as positive regulation of gene expression,inflammatory response,negative regulation of apoptosis,lipid and atherosclerosis pathways,AGE-RAGE signaling,fluid shear stress and atherosclerosis pathway,IL-17 signaling,and TNF signaling.Molecular docking demonstrated strong binding affinity between core components and targets,with stigmasterol exhibiting the highest binding activity.[Conclusions]Network pharmacology and clinical practice have demonstrated that Wuling Decoction exhibits remarkable efficacy and safety in the SS treatment.Through the synergistic effects of multiple components,multiple targets,and multiple pathways,it regulates immune,inflammatory,and endocrine signaling pathways,thereby improving the state of impaired triple-warmer qi transformation and fluid distribution.This provides both clinical and mechanistic evidence for treating the SS by TCM.展开更多
The problem of ulcerative colitis(UC)is becoming increasingly relevant due to its prevalence and combination with psychological disorders.UC is associated with alterations in the intestinal microbiocenosis as one of t...The problem of ulcerative colitis(UC)is becoming increasingly relevant due to its prevalence and combination with psychological disorders.UC is associated with alterations in the intestinal microbiocenosis as one of the factors reducing tolerance to self-antigens.In addition,patients with UC often experience anxiety,depression and other negative emotional and psychological factors aggravating the course of the underlying disease.The study presented by Wang et al shows that the traditional Chinese medicine Wuling can exert its therapeutic effect by reversing the aberrant precursor of brain-derived neurotrophic factor(BDNF)/BDNF signaling in mice with UC and depression.The combination of Wuling powder with mesalazine can exhibit an antidepressant effect and reduce intestinal inflammation.Undoubtedly,the study by Chinese authors deserves attention,and changes in the balance of precursor of BDNF/p75 neurotrophin receptor/sortilin and BDNF/tropomyosin receptor kinase B signaling may become a novel therapeutic target for clinical treatment and further development of research.展开更多
Five years＇ （2000-2004） continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the ...Five years＇ （2000-2004） continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the eastern part of the Wuling Mountains, south bank of the Three Gorges of Yangtze River in Hubei. A total of 29 297 rat clamps/times were placed and 2271 small mammals such as rodents were captured, and 26 small mammals were captured by other means. All the small mammals captured belonged to 8 families 19 genera and 24 species, of which rodentia accounted for 70.83% and insectivora 29.17%. Through analysis of the data, the results showed that： 1 ） although the species richness had a trend of increasing along different sample plots as altitude increased from south to north, quite a few species showed a wide habitat range in a vertical distribution （ 15 species were dispersed over three zones and two species over two zones） , indicating a strong adaptability of small mammals such as rOdents at lower altitudes in most areas and comparatively less vertical span of entire mountains; 2） whether in seven different sample plots or six different ecological types, Apodemus agrarius and Rattus norvegicus were dominant species below 1200m, and Anourosorex squamipes, Niviventer confucianus and Apodemus draco were dominant above altitudes of 1300m, however, in quantity they were short of identical regularity, meaning they did not increase as the altitude did, or decrease as the ecological areas changed; 3）the density in winter was obviously greater than that in spring, and the distribution showed an increasing trend along with altitude, but the density in different sample plots was short of identical regularity, showing changes in different seasons and altitude grades had an important impact on small mammals such as rodents; 4） in species diversity and evenness index, there were obvious changes between the seven different sample plots, probably caused by frequent human interference in this area. Comparatively speaking, there was less human interference at high altitudes where vegetation was rich and had a high diversity and evenness index, and the boundary effect and community stability were obvious. Most ecological types have been seriously interfered with due to excessive assart at low altitudes with singular vegetation and low diversity and evenness index and poor community stability, showing an ecosystem with poor anti-reversion. If human interference can be reduced in those communities at high altitudes with low diversity and evenness index, the biological diversity in the communities will gradually recover to similar levels of other ecological areas.展开更多
We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisa...We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B） in Liuwei Wuling tablet （LWWLT）. The HPLC baseline separation was carried out on an Inertsil ODS-3 column （250 mm×4.6 mm, 5 μm） at 25 ℃ with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0,999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.展开更多
Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used fo...Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used for constructing a core collection. Four evaluating parameters for maize landrace core collection, including mean difference percentage （MD）, variance difference percentage （VD）, coincidence rate of range （CR）, and variable rate of coefficient of variation （VR）, were assessed With 20 quantitative traits. It was shown that genetic relationships among landraces in Wuling Mountain region had the tendency to associate with their geographic origins. The 124 landraces were clustered into 18 subgroups when the coefficient of genetic similarity （GS） is 0.28. Eighteen landraces, each of which was from one subgroup, were applied to construct the core collection with a sampling percentage of 15%. Comparison of the initial and core collection indicated that there existed no significant differences in most quantitative traits. An average of 6.3 and 6.5 alleles were detected in the initial and core collection, respectively. Mean polymorphism information content in the core collection （0.75） was higher than that in the initial one （0.72）. MD was lesser than 20% and CR was more than 80%. The results showed that the sampling strategy would be feasible for constructing the core collection that well represents the genetic diversity of the initial one.展开更多
Osteoporosis is a health problem to cause global concern.A lot of methods have been used to prevent and treat osteoporosis,but there is still a lack of effective treatment for osteoporosis owing to limited understandi...Osteoporosis is a health problem to cause global concern.A lot of methods have been used to prevent and treat osteoporosis,but there is still a lack of effective treatment for osteoporosis owing to limited understanding of its mechanism.Therefore,the aim of this present study is to explore the underlying mechanism of Wuling Powder,a traditional Chinese medicine on treating osteoporosis.In this study,we firstly screened and identified the common targets between Wuling Powder and osteoporosis through the related databases,and then explored the relationships among these targets,Wuling Powder and osteoporosis by using Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and network analyses.Subsequently,the molecular docking was performed by using systemsDock to evaluate the potential binding relationships between the active components of Wuling Powder and their related targets.The results showed that in total of 14 common targets including CREBBP,ADAM17,GOT1,GAPDH,USP8,ERBB2,EEF1 A1,MTOR,RAC1,ETS1,DDX58,GCK,EGF and S100 A8 were screened.EGF,ERBB2,MTOR and HIF-1 were the potential therapeutic targets for osteoporosis,and they were also the related targets for predicting active components in Wuling Powder.Taken together,we concluded that Wuling Powder might be used to treat osteoporosis through above these targets.展开更多
In view of the importance of mafic dyke swarms and their contribution to current scientific problems relating to South China,herein,we present the findings of studies on twenty–five representative mafic dykes croppin...In view of the importance of mafic dyke swarms and their contribution to current scientific problems relating to South China,herein,we present the findings of studies on twenty–five representative mafic dykes cropping out in Hunan Province and Guangxi Zhuang Autonomous Region,within the southern Wuling Mountain gravity lineament,China.These results include new zircon LA-ICP-MS U-Pb age,whole rock geochemical,Sr-Nd-Pb isotopic,and zircon Hf isotopic data for these dykes.The dykes formed between 131.5±1.2 and 121.6±1.1 Ma,and have typical doleritic textures.They fall into the alkaline and shoshonitic series,are enriched in light rare earth elements(LREE),some large ion lithophile elements(LILE;e.g.,Rb,Ba,and Sr),Th,U,and Pb,and are depleted in Nb,Ta,Hf,and Ti.Moreover,the dolerites have high initial 87 Sr/86 Sr ratios(0.7055–0.7057),negativeεNd(t)and zirconεHf(t)values(-14.8 to-11.9,-30.4 to-14.9),and relatively constant initial Pb isotopic ratios(that are EM1-like,16.77–16.94,15.43–15.47,and 36.84–36.92 for 206 Pb/204 Pb,207 Pb/204 Pb,and 208 Pb/204 Pb,respectively).These results indicate that the dykes were likely derived from magma generated through low-degree partial melting(1.0%–10%)of an EM1-like garnet–lherzolite mantle source.The parental magmas fractionated olivine,clinopyroxene,plagioclase,and Ti-bearing phases with negligible crustal contamination,during ascent and dyke emplacement.Several possible models have been proposed to explain the origin of Mesozoic magmatism along the Wuling Mountain gravity lineament.Herein we propose a reasonable model for the origin of these mafic dykes,involving the collision between the paleo-Pacific Plate and South China,which led to subsequent lithospheric extension and asthenosphere upwelling,resulting in partial melting the underlying mantle lithosphere in the Early Cretaceous,to form the parental magmas to the WMGL mafic dykes,as studied.展开更多
BACKGROUND The treatment of adolescent patients with anxiety,depression and insomnia is challenging,and there is no ideal treatment method.AIM To evaluate the clinical efficacy of Wuling capsule combined with sertrali...BACKGROUND The treatment of adolescent patients with anxiety,depression and insomnia is challenging,and there is no ideal treatment method.AIM To evaluate the clinical efficacy of Wuling capsule combined with sertraline in the treatment of adolescent anxiety,depression and insomnia.METHODS Eighty adolescent patients with anxiety,depression with insomnia who were admitted to our hospital from April 1,2022 to March 30,2024.And the subjects were randomly classified into the control group(n=40)and the observational group(n=40).The control group was treated with a combination of sertraline and placebo.The observation group was treated with Wuling capsule in addition to sertraline.The two groups were cured continuously for 8 weeks.Insomnia severity index(ISI),Hamilton Anxiety Scale(HAMA)and Hamilton Depression Scale(HAMD)were used to evaluate the clinical symptoms before treatment and at 2,4,6 and 8 weeks after treatment.The Treatment Emergent Symptom Scale(TESS)was used to evaluate adverse reactions during treatment.RESULTS There was no obvious difference in HAMD,HAMA and ISI scores between the two groups before treatment(P>0.05).After treatment,the HAMD,HAMA and ISI scores of patients in both groups decreased compared with before treatment,and HAMD,HAMA and ISI scores of patients in the observation group were remarkedly lower than those in the control group at each time point after treatment(P<0.05).Compared with the control group,the TESS score of the study group were sharply lower(t=18.239,P<0.001).CONCLUSION Wuling capsule can further alleviate the insomnia symptoms of adolescents with anxiety and depression,and the efficacy and safety are high.It is recommended to promote the application.展开更多
Due to the difficult segmentation of land destiny, popularity destiny, industry destiny on the platform of common interests demand, the regional governance of production and living for the needs of the people in cross...Due to the difficult segmentation of land destiny, popularity destiny, industry destiny on the platform of common interests demand, the regional governance of production and living for the needs of the people in cross-regional areas, such as in the aspects of security, education, assignment and management of water resources, transportation, energy, environment protection and public health, the administrative border regional cooperation is relatively easy. These aspects are equivalent to public products. Just because of this, it leads to problems that nobody will not control. The governments, in order to realize regional economic coordinated development and to eliminate the regional interests parties of administrative regions economy in microcosmic, were adapting various self-protection measures, even including that "you may have your policy but I have my countermeasures" that is derived from inter-regional economic relations. The action of market segmentation has limited the flow of various economic development elements, especially the condition of "dead end highway", resource exploitation and use, difficult social security work in social living of the administrative boundary zone. This paper studies the typical economical district of Wuling mountain area in the obvious strategic position at the junction of Chongqing, Guizhou, Hunan, Hubei provinces from the angle of regional public management and public choice to present issue awareness and academic purpose.展开更多
The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,h...The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,has demonstrated efficacy in alleviating depressive symptoms.The purpose of this research was to explore the antidepressant properties of Wuling powder and its basic molecular effects,particularly regarding alterations in ER stress.A model of social defeat stress was created by introducing a mouse to the cage of an unfamiliar,hostile mouse for intervals of 5–10 min daily over a span of 10 d.Subsequently,the mice received oral doses of Wuling powder for 2 weeks.The social approach-avoidance assay was employed to evaluate signs of depression-like behaviors.Moreover,protein and gene expressions linked to ER stress triggered by social defeat were analyzed through Western blotting analysis and quantitative real-time PCR.The behavioral tests indicated that Wuling powder ameliorated behaviors associated with depression due to social defeat stress.Treatment with Wuling powder markedly reduced the increased levels of the 78-k Da glucose-regulated protein and protein disulfide isomerase caused by social defeat stress.It also diminished the expression of inositol-requiring enzyme 1α(IRE1α)and spliced X box-binding protein-1(s XBP1)at the protein and m RNA levels.Furthermore,Wuling treatment notably decreased the levels of phosphorylated eukaryotic initiation factor 2 alpha kinase(P-e IF2α),activating transcription factor 4(ATF4)and C/EBP homologous protein(CHOP),simultaneously enhancing the ratio of B-cell lymphoma 2(Bcl-2)to Bcl-2-associated X protein(Bax).These results suggested that Wuling powder could alleviate ER stress and inhibit cell apoptosis in the hippocampus by inhibiting protein translation and synthesis,thereby attenuating depressive-like behavior.展开更多
[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatmen...[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatment of diseases, so as to provide reference for scholars in this field in the future. [Methods] China National Knowledge Network(CNKI) database was used as the literature retrieval source to search the literature related to Wuling Powder in the treatment of nephropathy, and then the data visualization analysis was carried out by using the software citespace.5.7.R2. [Results] Through accurate retrieval, a total of 204 effective literatures were included. It was found that domestic research on the treatment of nephropathy by Wuling Powder was on the rise. From the analysis of knowledge graph, it was concluded that after long-term research and development, a relatively concentrated core research group had been formed in China, which mainly focused on "Wuling Powder", "chronic kidney disease" and "diabetic nephropathy". [Conclusions] The research on the treatment of nephropathy by Wuling Powder shows a good development trend, and the research content is constantly enriched, and this research is still the current and future research hotspot.展开更多
[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Data...[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Database,PubMed,Cochrane library and Embase,were retrieved,and comparative experiments of efficacy(serum uric acid level)and safety(incidence of adverse reactions)between Wuling powder(used singly or combined with other prescriptions)and conventional Western medicine in the treatment of hyperuricemia were included,and effect size was pooled with Revman 5.3.[Results]A total of 11 articles,involving 817 patients,were included,and their research quality was evaluated to be low.In terms of overall effect,Wuling powder(used singly or combined with other prescriptions)was better than the control drug in reducing serum uric acid level,but the difference was insignificant[SMD=-0.37,95%CI(-0.90,0.16)].The heterogeneity between studies was high.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group.Subgroup analysis shows that Wuling powder combined with other prescriptions was better than the control drug in reducing serum uric acid level[SMD=-0.37,95%CI(-0.90,0.16),P=0.17],and the heterogeneity was reduced.[Conclusions]Wuling powder combined with other prescriptions is better than conventional Western medicine in the treatment of hyperuricemia,and has better safety.Wuling powder alone is not as effective as conventional Western medicine.It is suggested that in clinical work,doctors may need to modify the prescription of Wuling powder.The low quality of the literature supporting this conclusion suggests that this evidence should be interpreted with caution.展开更多
[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal grou...[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal group,model group,prednisone acetate positive group,high-dose Wuling Powder group,medium-dose Wuling Power group and low-dose Wuling Power group,with 10 mice in each group.Three days after prophylactic administration,a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution(7.5 mg/kg)into the tail vein.After successful modeling,prednisone acetate and Wuling SAN were given high,medium and low doses for intervention for 28 d,respectively.After that,urinary protein and creatinine contents of mice in each group were detected,and pathological damage of renal tissue was observed by HE and Masson staining.The mRNA levels of Notch1,Jagged1 and Hes1 in mouse kidney tissues were detected by RT-PCR,and the expression levels of Notch1,Jagged1 and Hes1 proteins were detected by Western blot.[Results]Wuling Powder could effectively reduce the contents of urine protein(P<0.01)and Scr(P<0.01)in NS mice,and alleviate the pathological injury of kidney.Compared with the model group,the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notch1,Jagged1 and Hes1 mRNA in the kidney tissue of mice(P<0.01),and the expression of Notch1 protein in the renal tissue of mice decreased(P<0.01).The contents of Hes1 in the prednisone acetate group and the high-and medium-dose Wuling Powder groups significantly decreased(P<0.05).[Conclusions]Wuling Powder could protect the kidneys in mice with NS through Notch pathway.展开更多
Background Myocardial ischemia-reperfusion injury is characterized by the inability of tissue and organ function to recover after the perfusion blood flow is restored followed by myocardial ischemia.Previous studies h...Background Myocardial ischemia-reperfusion injury is characterized by the inability of tissue and organ function to recover after the perfusion blood flow is restored followed by myocardial ischemia.Previous studies have shown that many Chinese herbal compounds can reduce myocardial ischemia-reperfusion injury,but the efficacy of Wuling Powder in treating myocardial ischemia-reperfusion injury has not been reported.The present study aimed to investigate the mechanism of Wuling Powder in treating myocardial ischemia-reperfusion injury by network pharmacology and molecular docking.Methods The active constituents of Wuling Powder were obtained by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the related targets of active constituents of Wuling Powder were screened by the Swiss Target Prediction database.Disease targets of myocardial ischemia-reperfusion injury were obtained by Gene Cards,Dis Ge NET and OMIM gene databases.The active compound-target network of Wuling Powder was constructed using Cytoscape software.The drug prediction targets were mapped to the disease target set,and the intersection targets were obtained.The Gene Ontology(GO)function and Kyoto Encyclopedia of Genes anf Genomes(KEGG)pathway enrichment analysis were performed on the intersection targets obtained by using String database.Auto Dock Vina was used for molecular docking of core targets and the top 3 key active ingredients with degree values.Results Through screening,56 active ingredients and 116 targets of Wuling Powder were obtained.There were 27 common targets of Wuling Powder and myocardial ischemia-reperfusion injury in Myocardial ischemia-reperfusion injury(MIRI).Protein-Protein Interaction(PPI)network analysis in this study showed that CASP3,PTGS2,CAT,JUN,ESR1,CASP8,CASP9 and RELA may be the key targets of Wuling Powder to exert the protective effect of MIRI.GO functional analysis and KEGG pathway enrichment analysis showed that biological processes such as apoptosis,lipopolysaccharide response,steroid hormone response and signal transduction pathways such as PTGS2,TNF-α,nuclear transcription factor-κB(NF-κB),MAPK,PI3K/AKT,NLRP3,and autophagy play an important role in the occurrence and development of MIRI.Molecular docking results showed thatβ-sitosterol and hederagenin had good binding activities with key targets such as PTGS2,CASP3,and CAT.Conclusions Wuling Powder may act on CASP3,PTGS,CAT,JUN,ESR1,CASP8,CASP9 and other targets throughβ-sitosterol,hederagenin,3β-acetoxyatractylone,taxifolin and other active ingredients.[S Chin J Cardiol 2024;25(3):179-192]展开更多
Objective To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet（LWT）. Methods Reversed phase HPLC method was used with variously improved conditions. Results...Objective To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet（LWT）. Methods Reversed phase HPLC method was used with variously improved conditions. Results Salidroside, forsythoside A, specnuezhenide, phillyrin, schisandrol A, schizandrol A, schizandrin A, and schizandrin B in LWT were successfully separated on a Kromasil 100-5-C_（18） reverse phase column（250 mm × 4.6 mm, 5 μm） using acetonitrile -0.1% phosphoric acid（gradient elution） as mobile phase. The detection wavelength was 275 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 35 ℃. The recovery rate of the method was within the range of 95.13%-104.56% and the precision（RSD） was less than 3% for all eight analytes. All the compounds showed good linearities（r^2〉0.9980） in a relatively wide concentration range. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation of LWT.展开更多
As an important part of South China Old Land, the Jiangnan Orogenic Belt plays a significant role in explaining the assembly and the evolution of the Upper Yangtze Block and Cathaysia, as well as the structure and gro...As an important part of South China Old Land, the Jiangnan Orogenic Belt plays a significant role in explaining the assembly and the evolution of the Upper Yangtze Block and Cathaysia, as well as the structure and growth mechanism of continental lithosphere in South China.The Lengjiaxi and the Banxi groups are the base strata of the west section of the Jiangnan Orogenic Belt.Thus, the research of geochronology and tectonic evolution of the Lengjiaxi and the Banxi groups is significant.The maximum sedimentary age of the Lengjiaxi Group is ca.862 Ma, and the minimum is ca.822 Ma.The Zhangjiawan Formation, which is situated in the upper part of the Banxi Group is ca.802 Ma.The Lengjiaxi Group and equivalent strata should thus belong to the Neoproterozoic in age.The Jiangnan Orogenic Belt consisting of the Lengjiaxi and the Banxi groups as important constituents is not a Greenville Orogen Belt（1.3 Ga–1.0 Ga）.The Jiangnan Orogenic Belt is a recyclic orogenic belt, and the prototype basin is a foreland basin with materials derived from the southwest and the sediments belong to the active continental sedimentation.By combining large amounts of dating data of the Lengjiaxi and the Banxi groups as well as equivalent strata, the evolutionary model of the western section of the Jiangnan Orogenic Belt is established as follows： Before 862 Ma, the South China Ocean was subducted beneath the Upper Yangtze Block, while a continental island arc was formed on the side near the Upper Yangtze Block.The South China Ocean was not closed in this period.From 862 Ma to 822 Ma, the Upper Yangtze Block was collided with Cathaysia; and sediments began to be deposited in the foreland basin between the two blocks.The Lengjiaxi Group and equivalent strata were thus formed and the materials might be derived from the recyclic orogenic belt.From 822 Ma to 802 Ma, Cathaysia continued pushing to the Upper Yangtze Block, experienced the Jinning-Sibao Movement（Wuling Movement）; as result, the folded basement of the Jiangnan Orogenic Belt was formed.After 802 Ma, Cathaysia and the Upper Yangtze Block were separated from each other, the Nanhua rift basin was formed and began to receive the sediments of the Banxi Group and equivalent strata.These large amounts of dating data and research results also indicate that before the collision of the Upper Yangtze Block with Cathaysia, materials of the continental crust became less and less from the southwest to the east in the Jiangnan Orogeneic Belt; only island arc and neomagmatic arc were developed in the eastern section.Ocean-continent subduction or continent-continent subduction took place in the western and southern sections, while intra-oceanic subduction occurred in the eastern section.Comprehensive analyses on U-Pb ages and Hf model ages of zircons, the main provenance of the Lengjiaxi Group is Cathaysia.展开更多
[Objective] The aim was to to enhance the technology to brew better the traditional grape wine, which provided theoretical basis for the development of Wuling Mountain grape wine. [Method] Taking grapes in Wuling Moun...[Objective] The aim was to to enhance the technology to brew better the traditional grape wine, which provided theoretical basis for the development of Wuling Mountain grape wine. [Method] Taking grapes in Wuling Mountain as the row materials, Saccharomyces cerevisiae of different wines was chosen and brewed. The quality of grape wine was studied and the sensory evaluation was analyzed by dint of GC-MS. [Result] Result illustrated that the ethanol concentration of the wine brewed by exploration craft A was 68.5 g/L and that brewed by exploration craft B was 59.5 g/L; the higher alcohol content of craft A was 37.86% while that of craft B is 35.99%; craft A's percentage content of esters was 28.82% while that of B was 27.10%; craft A's percentage content of acids was 2.20% while that of B was 1.24%; the gamma-aminobutyric acid content of craft A was 33.2 g/100 ml while that of craft B was 35.4 g/100 ml. the essential amino-acid content of craft A was 11.8 g/100 ml while that of craft B was 13.9 g/100 ml .The result indicates that the wine brewed by craft A was aromatic and had special flavor. [Conclusion] In this case, the grape wine has particular flavor and has good property, which can be exploited.展开更多
Fanjing Mountain in Tongren City,Guizhou Province.As the main summit of the Wuling Mountain Range,Fanjing stands at an elevation of 2,572 meters.It was one of the earliest landmasses in southern China to rise from the...Fanjing Mountain in Tongren City,Guizhou Province.As the main summit of the Wuling Mountain Range,Fanjing stands at an elevation of 2,572 meters.It was one of the earliest landmasses in southern China to rise from the ocean and stillretains geological wonders dating back 1.4 billion years.展开更多
文摘BACKGROUND Ulcerative colitis(UC)is a chronic inflammatory disease affecting the colon.The most common psychological issue in UC patients is varying degrees of depre-ssion,which affects the condition and quality of life of UC patients and may lead to deterioration of the patient’s condition.UC drugs combined with anti-anxiety and antidepression drugs can alleviate symptoms of both depression and UC.Brain-derived neurotrophic factor(BDNF)precursor(proBDNF)/p75 neurotrophin receptor(p75NTR)/sortilin and BDNF/tropomyosin receptor kinase B(TrkB)signalling balance is essential for maintaining brain homeostasis and preventing the development of depressive behaviours.AIM To explore the mechanism by which Wuling powder regulates the proBDNF/p75NTR/sortilin and BDNF/TrkB pathways in the treatment of UC with depre-ssion.METHODS Depression was established in C57BL/6J mice via chronic restraint stress,and the UC model was induced with dextran sodium sulfate(DSS).In the treatment stage,mesalazine(MS)was the basic treatment,Wuling powder was the experimental treatment,and fluoxetine was the positive control drug for treating depression.Changes in intestinal mucosal inflammation,behaviour,and the proBDNFp75NTR/sortilin and BDNF/TrkB pathways were evaluated.RESULTS In the depression groups,Wuling powder decreased the immobility time,increased the distance travelled in the central zone and the total distance travelled,and restored balance in the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways.In the DSS and chronic restraint stress+DSS groups,immobility time increased,distance travelled in the central zone and total distance travelled decreased,activity of the proBDNF/p75NTR/sortilin pathway was upregulated,and activity of the BDNF/TrkB pathway was downregulated,indicating that mice with UC often have comorbid depression.Compared with those of MS alone,Wuling powder combined with MS further decreased the colon histopathological scores and the expression levels of tumor necrosis factor-alpha and interleukin-6 mRNAs.CONCLUSION This study confirmed that Wuling powder may play an antidepressant role by regulating the balance of the proBDNF/p75NTR/sortilin and BDNF/TrkB signalling pathways and further relieve intestinal inflammation in UC.
基金Supported by Research Project of Shanxi Provincial Administration of Traditional Chinese Medicine-"Distributing Fluids and Retaining Moisture Method in Treating Sj gren s Syndrome Based on Real-World Study"(2022ZYYC010)Discipline Construction Project of Shanxi University of Chinese Medicine-Internal Medicine of Chinese Medicine(2025XK02).
文摘[Objectives]To explore the action mechanism of Wuling Decoction(the combination of Wumei Pill and Wuling Powder)in treating Sjögren's syndrome(SS)based on network pharmacology and molecular docking techniques.[Methods]The active components and their targets of Wuling Decoction were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The targets related to SS were obtained from the GeneCards database.The intersection targets between Wuling Decoction and SS were identified using the Venny platform.The"Chinese medicinal-active component-common target"network was constructed using Cytoscape.The potential targets were improted into STRING to establish the protein-protein interaction(PPI)network,and the topological parameters were evaluated using Cytoscape to determine the key targets.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed using the DAVID database.Molecular docking of core components and key targets was conducted using the CB-Dock2 online tool.[Results]Wuling Decoction contained 121 active components of Chinese medicinals and 190 related targets.There were 1447 SS-related targets obtained from GeneCards databases.50 potential targets of Wuling decoction for SS were obtained.Network topology analysis revealed that quercetin,stigmasterol,andβ-sitosterol were core components.PPI network analysis indicated that TNF,IL-6,and IL-1βwere key targets.These targets were primarily involved in biological processes such as positive regulation of gene expression,inflammatory response,negative regulation of apoptosis,lipid and atherosclerosis pathways,AGE-RAGE signaling,fluid shear stress and atherosclerosis pathway,IL-17 signaling,and TNF signaling.Molecular docking demonstrated strong binding affinity between core components and targets,with stigmasterol exhibiting the highest binding activity.[Conclusions]Network pharmacology and clinical practice have demonstrated that Wuling Decoction exhibits remarkable efficacy and safety in the SS treatment.Through the synergistic effects of multiple components,multiple targets,and multiple pathways,it regulates immune,inflammatory,and endocrine signaling pathways,thereby improving the state of impaired triple-warmer qi transformation and fluid distribution.This provides both clinical and mechanistic evidence for treating the SS by TCM.
文摘The problem of ulcerative colitis(UC)is becoming increasingly relevant due to its prevalence and combination with psychological disorders.UC is associated with alterations in the intestinal microbiocenosis as one of the factors reducing tolerance to self-antigens.In addition,patients with UC often experience anxiety,depression and other negative emotional and psychological factors aggravating the course of the underlying disease.The study presented by Wang et al shows that the traditional Chinese medicine Wuling can exert its therapeutic effect by reversing the aberrant precursor of brain-derived neurotrophic factor(BDNF)/BDNF signaling in mice with UC and depression.The combination of Wuling powder with mesalazine can exhibit an antidepressant effect and reduce intestinal inflammation.Undoubtedly,the study by Chinese authors deserves attention,and changes in the balance of precursor of BDNF/p75 neurotrophin receptor/sortilin and BDNF/tropomyosin receptor kinase B signaling may become a novel therapeutic target for clinical treatment and further development of research.
基金National Natural Science Foundation of China(30070679)the Natural Science Foundation of Hubei Province(2004ABA138)+1 种基金the Key Technology R&D Programme Foundation of Hubei Province(2002AA301C43)the Hubei Health Bureau Research Programme Foundation(NX200427)
文摘Five years＇ （2000-2004） continuous study has been carried out on small mammals such as rodents in seven different sample plots, at three different altitudes and in six different ecological environment types in the eastern part of the Wuling Mountains, south bank of the Three Gorges of Yangtze River in Hubei. A total of 29 297 rat clamps/times were placed and 2271 small mammals such as rodents were captured, and 26 small mammals were captured by other means. All the small mammals captured belonged to 8 families 19 genera and 24 species, of which rodentia accounted for 70.83% and insectivora 29.17%. Through analysis of the data, the results showed that： 1 ） although the species richness had a trend of increasing along different sample plots as altitude increased from south to north, quite a few species showed a wide habitat range in a vertical distribution （ 15 species were dispersed over three zones and two species over two zones） , indicating a strong adaptability of small mammals such as rOdents at lower altitudes in most areas and comparatively less vertical span of entire mountains; 2） whether in seven different sample plots or six different ecological types, Apodemus agrarius and Rattus norvegicus were dominant species below 1200m, and Anourosorex squamipes, Niviventer confucianus and Apodemus draco were dominant above altitudes of 1300m, however, in quantity they were short of identical regularity, meaning they did not increase as the altitude did, or decrease as the ecological areas changed; 3）the density in winter was obviously greater than that in spring, and the distribution showed an increasing trend along with altitude, but the density in different sample plots was short of identical regularity, showing changes in different seasons and altitude grades had an important impact on small mammals such as rodents; 4） in species diversity and evenness index, there were obvious changes between the seven different sample plots, probably caused by frequent human interference in this area. Comparatively speaking, there was less human interference at high altitudes where vegetation was rich and had a high diversity and evenness index, and the boundary effect and community stability were obvious. Most ecological types have been seriously interfered with due to excessive assart at low altitudes with singular vegetation and low diversity and evenness index and poor community stability, showing an ecosystem with poor anti-reversion. If human interference can be reduced in those communities at high altitudes with low diversity and evenness index, the biological diversity in the communities will gradually recover to similar levels of other ecological areas.
基金State Project for Essential Drug Research and Development(Grant No.2012ZX09J12108-04C)Special Project of Military Health Ministry(Grant No.13ZJZ12-5)
文摘We employed high performance liquid chromatography （HPLC） method coupled with diode array detector （DAD） for the determination of seven major bioactive compounds （forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B） in Liuwei Wuling tablet （LWWLT）. The HPLC baseline separation was carried out on an Inertsil ODS-3 column （250 mm×4.6 mm, 5 μm） at 25 ℃ with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0,999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.
基金the National High Tech-nology Research and Development Program of China(863 Program, 2004BA525B04)Program for Changjiang Scholars and Innovative Research Team in University of China (IRTO453)
文摘Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used for constructing a core collection. Four evaluating parameters for maize landrace core collection, including mean difference percentage （MD）, variance difference percentage （VD）, coincidence rate of range （CR）, and variable rate of coefficient of variation （VR）, were assessed With 20 quantitative traits. It was shown that genetic relationships among landraces in Wuling Mountain region had the tendency to associate with their geographic origins. The 124 landraces were clustered into 18 subgroups when the coefficient of genetic similarity （GS） is 0.28. Eighteen landraces, each of which was from one subgroup, were applied to construct the core collection with a sampling percentage of 15%. Comparison of the initial and core collection indicated that there existed no significant differences in most quantitative traits. An average of 6.3 and 6.5 alleles were detected in the initial and core collection, respectively. Mean polymorphism information content in the core collection （0.75） was higher than that in the initial one （0.72）. MD was lesser than 20% and CR was more than 80%. The results showed that the sampling strategy would be feasible for constructing the core collection that well represents the genetic diversity of the initial one.
基金supported by the National Natural Science Foundation of China(No.81560777)the Innovation Project of Guangxi Graduate Education(No.YCSZ20190019)。
文摘Osteoporosis is a health problem to cause global concern.A lot of methods have been used to prevent and treat osteoporosis,but there is still a lack of effective treatment for osteoporosis owing to limited understanding of its mechanism.Therefore,the aim of this present study is to explore the underlying mechanism of Wuling Powder,a traditional Chinese medicine on treating osteoporosis.In this study,we firstly screened and identified the common targets between Wuling Powder and osteoporosis through the related databases,and then explored the relationships among these targets,Wuling Powder and osteoporosis by using Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and network analyses.Subsequently,the molecular docking was performed by using systemsDock to evaluate the potential binding relationships between the active components of Wuling Powder and their related targets.The results showed that in total of 14 common targets including CREBBP,ADAM17,GOT1,GAPDH,USP8,ERBB2,EEF1 A1,MTOR,RAC1,ETS1,DDX58,GCK,EGF and S100 A8 were screened.EGF,ERBB2,MTOR and HIF-1 were the potential therapeutic targets for osteoporosis,and they were also the related targets for predicting active components in Wuling Powder.Taken together,we concluded that Wuling Powder might be used to treat osteoporosis through above these targets.
基金supported by the National Natural Science Foundation of China(grant,41573022)。
文摘In view of the importance of mafic dyke swarms and their contribution to current scientific problems relating to South China,herein,we present the findings of studies on twenty–five representative mafic dykes cropping out in Hunan Province and Guangxi Zhuang Autonomous Region,within the southern Wuling Mountain gravity lineament,China.These results include new zircon LA-ICP-MS U-Pb age,whole rock geochemical,Sr-Nd-Pb isotopic,and zircon Hf isotopic data for these dykes.The dykes formed between 131.5±1.2 and 121.6±1.1 Ma,and have typical doleritic textures.They fall into the alkaline and shoshonitic series,are enriched in light rare earth elements(LREE),some large ion lithophile elements(LILE;e.g.,Rb,Ba,and Sr),Th,U,and Pb,and are depleted in Nb,Ta,Hf,and Ti.Moreover,the dolerites have high initial 87 Sr/86 Sr ratios(0.7055–0.7057),negativeεNd(t)and zirconεHf(t)values(-14.8 to-11.9,-30.4 to-14.9),and relatively constant initial Pb isotopic ratios(that are EM1-like,16.77–16.94,15.43–15.47,and 36.84–36.92 for 206 Pb/204 Pb,207 Pb/204 Pb,and 208 Pb/204 Pb,respectively).These results indicate that the dykes were likely derived from magma generated through low-degree partial melting(1.0%–10%)of an EM1-like garnet–lherzolite mantle source.The parental magmas fractionated olivine,clinopyroxene,plagioclase,and Ti-bearing phases with negligible crustal contamination,during ascent and dyke emplacement.Several possible models have been proposed to explain the origin of Mesozoic magmatism along the Wuling Mountain gravity lineament.Herein we propose a reasonable model for the origin of these mafic dykes,involving the collision between the paleo-Pacific Plate and South China,which led to subsequent lithospheric extension and asthenosphere upwelling,resulting in partial melting the underlying mantle lithosphere in the Early Cretaceous,to form the parental magmas to the WMGL mafic dykes,as studied.
文摘BACKGROUND The treatment of adolescent patients with anxiety,depression and insomnia is challenging,and there is no ideal treatment method.AIM To evaluate the clinical efficacy of Wuling capsule combined with sertraline in the treatment of adolescent anxiety,depression and insomnia.METHODS Eighty adolescent patients with anxiety,depression with insomnia who were admitted to our hospital from April 1,2022 to March 30,2024.And the subjects were randomly classified into the control group(n=40)and the observational group(n=40).The control group was treated with a combination of sertraline and placebo.The observation group was treated with Wuling capsule in addition to sertraline.The two groups were cured continuously for 8 weeks.Insomnia severity index(ISI),Hamilton Anxiety Scale(HAMA)and Hamilton Depression Scale(HAMD)were used to evaluate the clinical symptoms before treatment and at 2,4,6 and 8 weeks after treatment.The Treatment Emergent Symptom Scale(TESS)was used to evaluate adverse reactions during treatment.RESULTS There was no obvious difference in HAMD,HAMA and ISI scores between the two groups before treatment(P>0.05).After treatment,the HAMD,HAMA and ISI scores of patients in both groups decreased compared with before treatment,and HAMD,HAMA and ISI scores of patients in the observation group were remarkedly lower than those in the control group at each time point after treatment(P<0.05).Compared with the control group,the TESS score of the study group were sharply lower(t=18.239,P<0.001).CONCLUSION Wuling capsule can further alleviate the insomnia symptoms of adolescents with anxiety and depression,and the efficacy and safety are high.It is recommended to promote the application.
基金supported by National Social Science Fund of China (Grant No.09XJY011)Natural Science Foundation of Chongqing Normal University (Grant No.26X2602)
文摘Due to the difficult segmentation of land destiny, popularity destiny, industry destiny on the platform of common interests demand, the regional governance of production and living for the needs of the people in cross-regional areas, such as in the aspects of security, education, assignment and management of water resources, transportation, energy, environment protection and public health, the administrative border regional cooperation is relatively easy. These aspects are equivalent to public products. Just because of this, it leads to problems that nobody will not control. The governments, in order to realize regional economic coordinated development and to eliminate the regional interests parties of administrative regions economy in microcosmic, were adapting various self-protection measures, even including that "you may have your policy but I have my countermeasures" that is derived from inter-regional economic relations. The action of market segmentation has limited the flow of various economic development elements, especially the condition of "dead end highway", resource exploitation and use, difficult social security work in social living of the administrative boundary zone. This paper studies the typical economical district of Wuling mountain area in the obvious strategic position at the junction of Chongqing, Guizhou, Hunan, Hubei provinces from the angle of regional public management and public choice to present issue awareness and academic purpose.
基金National Natural Science Foundation of China(Grant No.81100801 and 81050025)the foundation of Zhejiang Jolly Pharmaceutical Co.,Ltd.
文摘The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,has demonstrated efficacy in alleviating depressive symptoms.The purpose of this research was to explore the antidepressant properties of Wuling powder and its basic molecular effects,particularly regarding alterations in ER stress.A model of social defeat stress was created by introducing a mouse to the cage of an unfamiliar,hostile mouse for intervals of 5–10 min daily over a span of 10 d.Subsequently,the mice received oral doses of Wuling powder for 2 weeks.The social approach-avoidance assay was employed to evaluate signs of depression-like behaviors.Moreover,protein and gene expressions linked to ER stress triggered by social defeat were analyzed through Western blotting analysis and quantitative real-time PCR.The behavioral tests indicated that Wuling powder ameliorated behaviors associated with depression due to social defeat stress.Treatment with Wuling powder markedly reduced the increased levels of the 78-k Da glucose-regulated protein and protein disulfide isomerase caused by social defeat stress.It also diminished the expression of inositol-requiring enzyme 1α(IRE1α)and spliced X box-binding protein-1(s XBP1)at the protein and m RNA levels.Furthermore,Wuling treatment notably decreased the levels of phosphorylated eukaryotic initiation factor 2 alpha kinase(P-e IF2α),activating transcription factor 4(ATF4)and C/EBP homologous protein(CHOP),simultaneously enhancing the ratio of B-cell lymphoma 2(Bcl-2)to Bcl-2-associated X protein(Bax).These results suggested that Wuling powder could alleviate ER stress and inhibit cell apoptosis in the hippocampus by inhibiting protein translation and synthesis,thereby attenuating depressive-like behavior.
基金Supported by Open Project for First-class Discipline Construction in Guangxi(2019XK120)Natural Science Research Project of Guangxi University of Chinese Medicine (2018MS008)。
文摘[Objectives]This study was conducted to systematically present the research and development status of Wuling Powder over the years, and comprehensively analyze the hot spots and trends of Wuling Powder in the treatment of diseases, so as to provide reference for scholars in this field in the future. [Methods] China National Knowledge Network(CNKI) database was used as the literature retrieval source to search the literature related to Wuling Powder in the treatment of nephropathy, and then the data visualization analysis was carried out by using the software citespace.5.7.R2. [Results] Through accurate retrieval, a total of 204 effective literatures were included. It was found that domestic research on the treatment of nephropathy by Wuling Powder was on the rise. From the analysis of knowledge graph, it was concluded that after long-term research and development, a relatively concentrated core research group had been formed in China, which mainly focused on "Wuling Powder", "chronic kidney disease" and "diabetic nephropathy". [Conclusions] The research on the treatment of nephropathy by Wuling Powder shows a good development trend, and the research content is constantly enriched, and this research is still the current and future research hotspot.
基金Supported by China Postdoctoral Science Foundation(2014M550133)Construction of Biostatistics Course Based on Mixed Teaching Platform(JYB20180724)Product Development and Cultural Dissemination of Traditional Chinese Medicine Preparations(20200107).
文摘[Objectives]To compare the clinical efficacy and safety of Wuling powder and conventional Western medicine for lowering uric acid in the treatment of hyperuricemia.[Methods]Databases such as CNKI,Wanfang Data,VIP Database,PubMed,Cochrane library and Embase,were retrieved,and comparative experiments of efficacy(serum uric acid level)and safety(incidence of adverse reactions)between Wuling powder(used singly or combined with other prescriptions)and conventional Western medicine in the treatment of hyperuricemia were included,and effect size was pooled with Revman 5.3.[Results]A total of 11 articles,involving 817 patients,were included,and their research quality was evaluated to be low.In terms of overall effect,Wuling powder(used singly or combined with other prescriptions)was better than the control drug in reducing serum uric acid level,but the difference was insignificant[SMD=-0.37,95%CI(-0.90,0.16)].The heterogeneity between studies was high.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group.Subgroup analysis shows that Wuling powder combined with other prescriptions was better than the control drug in reducing serum uric acid level[SMD=-0.37,95%CI(-0.90,0.16),P=0.17],and the heterogeneity was reduced.[Conclusions]Wuling powder combined with other prescriptions is better than conventional Western medicine in the treatment of hyperuricemia,and has better safety.Wuling powder alone is not as effective as conventional Western medicine.It is suggested that in clinical work,doctors may need to modify the prescription of Wuling powder.The low quality of the literature supporting this conclusion suggests that this evidence should be interpreted with caution.
基金Supported by Natural Science Research of the Open Project of First-class Discipline Construction in Guangxi(2019XK120)Natural Science Research Project of Guangxi University of Chinese Medicine(2018MS008)College Students’Innovation and Entrepreneurship Training Program of Guangxi Autonomous Region in 2023(S202310600102).
文摘[Objectives]This study was conducted to investigate the renal protective effects of Wuling Powder on mice with nephrotic syndrome(NS)based on Notch pathway.[Methods]Sixty KM mice were randomly divided into normal group,model group,prednisone acetate positive group,high-dose Wuling Powder group,medium-dose Wuling Power group and low-dose Wuling Power group,with 10 mice in each group.Three days after prophylactic administration,a comprehensive nephropathy model was prepared by injecting 1 mg/ml doxorubicin hydrochloride solution(7.5 mg/kg)into the tail vein.After successful modeling,prednisone acetate and Wuling SAN were given high,medium and low doses for intervention for 28 d,respectively.After that,urinary protein and creatinine contents of mice in each group were detected,and pathological damage of renal tissue was observed by HE and Masson staining.The mRNA levels of Notch1,Jagged1 and Hes1 in mouse kidney tissues were detected by RT-PCR,and the expression levels of Notch1,Jagged1 and Hes1 proteins were detected by Western blot.[Results]Wuling Powder could effectively reduce the contents of urine protein(P<0.01)and Scr(P<0.01)in NS mice,and alleviate the pathological injury of kidney.Compared with the model group,the prednisone acetate group and various Wuling Powder groups could down-regulate the expressions of Notch1,Jagged1 and Hes1 mRNA in the kidney tissue of mice(P<0.01),and the expression of Notch1 protein in the renal tissue of mice decreased(P<0.01).The contents of Hes1 in the prednisone acetate group and the high-and medium-dose Wuling Powder groups significantly decreased(P<0.05).[Conclusions]Wuling Powder could protect the kidneys in mice with NS through Notch pathway.
基金supported by grants from the National Nature Foundation Youth Program(No.82002095)。
文摘Background Myocardial ischemia-reperfusion injury is characterized by the inability of tissue and organ function to recover after the perfusion blood flow is restored followed by myocardial ischemia.Previous studies have shown that many Chinese herbal compounds can reduce myocardial ischemia-reperfusion injury,but the efficacy of Wuling Powder in treating myocardial ischemia-reperfusion injury has not been reported.The present study aimed to investigate the mechanism of Wuling Powder in treating myocardial ischemia-reperfusion injury by network pharmacology and molecular docking.Methods The active constituents of Wuling Powder were obtained by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the related targets of active constituents of Wuling Powder were screened by the Swiss Target Prediction database.Disease targets of myocardial ischemia-reperfusion injury were obtained by Gene Cards,Dis Ge NET and OMIM gene databases.The active compound-target network of Wuling Powder was constructed using Cytoscape software.The drug prediction targets were mapped to the disease target set,and the intersection targets were obtained.The Gene Ontology(GO)function and Kyoto Encyclopedia of Genes anf Genomes(KEGG)pathway enrichment analysis were performed on the intersection targets obtained by using String database.Auto Dock Vina was used for molecular docking of core targets and the top 3 key active ingredients with degree values.Results Through screening,56 active ingredients and 116 targets of Wuling Powder were obtained.There were 27 common targets of Wuling Powder and myocardial ischemia-reperfusion injury in Myocardial ischemia-reperfusion injury(MIRI).Protein-Protein Interaction(PPI)network analysis in this study showed that CASP3,PTGS2,CAT,JUN,ESR1,CASP8,CASP9 and RELA may be the key targets of Wuling Powder to exert the protective effect of MIRI.GO functional analysis and KEGG pathway enrichment analysis showed that biological processes such as apoptosis,lipopolysaccharide response,steroid hormone response and signal transduction pathways such as PTGS2,TNF-α,nuclear transcription factor-κB(NF-κB),MAPK,PI3K/AKT,NLRP3,and autophagy play an important role in the occurrence and development of MIRI.Molecular docking results showed thatβ-sitosterol and hederagenin had good binding activities with key targets such as PTGS2,CASP3,and CAT.Conclusions Wuling Powder may act on CASP3,PTGS,CAT,JUN,ESR1,CASP8,CASP9 and other targets throughβ-sitosterol,hederagenin,3β-acetoxyatractylone,taxifolin and other active ingredients.[S Chin J Cardiol 2024;25(3):179-192]
基金National Natural Science Foundation of China(No.81330090)National Natural Science Foundation of China(No.81274026)National Natural Science Foundation of China(No.03772888)
文摘Objective To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet（LWT）. Methods Reversed phase HPLC method was used with variously improved conditions. Results Salidroside, forsythoside A, specnuezhenide, phillyrin, schisandrol A, schizandrol A, schizandrin A, and schizandrin B in LWT were successfully separated on a Kromasil 100-5-C_（18） reverse phase column（250 mm × 4.6 mm, 5 μm） using acetonitrile -0.1% phosphoric acid（gradient elution） as mobile phase. The detection wavelength was 275 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 35 ℃. The recovery rate of the method was within the range of 95.13%-104.56% and the precision（RSD） was less than 3% for all eight analytes. All the compounds showed good linearities（r^2〉0.9980） in a relatively wide concentration range. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation of LWT.
基金supported by National Major Projects of Oil and Gas (2011ZX05043-005)Geological Survey Project of China Geological Survey (1212011120115, 1212011120117 and 121201011120131)Natural Science Fund Project (40921062)
文摘As an important part of South China Old Land, the Jiangnan Orogenic Belt plays a significant role in explaining the assembly and the evolution of the Upper Yangtze Block and Cathaysia, as well as the structure and growth mechanism of continental lithosphere in South China.The Lengjiaxi and the Banxi groups are the base strata of the west section of the Jiangnan Orogenic Belt.Thus, the research of geochronology and tectonic evolution of the Lengjiaxi and the Banxi groups is significant.The maximum sedimentary age of the Lengjiaxi Group is ca.862 Ma, and the minimum is ca.822 Ma.The Zhangjiawan Formation, which is situated in the upper part of the Banxi Group is ca.802 Ma.The Lengjiaxi Group and equivalent strata should thus belong to the Neoproterozoic in age.The Jiangnan Orogenic Belt consisting of the Lengjiaxi and the Banxi groups as important constituents is not a Greenville Orogen Belt（1.3 Ga–1.0 Ga）.The Jiangnan Orogenic Belt is a recyclic orogenic belt, and the prototype basin is a foreland basin with materials derived from the southwest and the sediments belong to the active continental sedimentation.By combining large amounts of dating data of the Lengjiaxi and the Banxi groups as well as equivalent strata, the evolutionary model of the western section of the Jiangnan Orogenic Belt is established as follows： Before 862 Ma, the South China Ocean was subducted beneath the Upper Yangtze Block, while a continental island arc was formed on the side near the Upper Yangtze Block.The South China Ocean was not closed in this period.From 862 Ma to 822 Ma, the Upper Yangtze Block was collided with Cathaysia; and sediments began to be deposited in the foreland basin between the two blocks.The Lengjiaxi Group and equivalent strata were thus formed and the materials might be derived from the recyclic orogenic belt.From 822 Ma to 802 Ma, Cathaysia continued pushing to the Upper Yangtze Block, experienced the Jinning-Sibao Movement（Wuling Movement）; as result, the folded basement of the Jiangnan Orogenic Belt was formed.After 802 Ma, Cathaysia and the Upper Yangtze Block were separated from each other, the Nanhua rift basin was formed and began to receive the sediments of the Banxi Group and equivalent strata.These large amounts of dating data and research results also indicate that before the collision of the Upper Yangtze Block with Cathaysia, materials of the continental crust became less and less from the southwest to the east in the Jiangnan Orogeneic Belt; only island arc and neomagmatic arc were developed in the eastern section.Ocean-continent subduction or continent-continent subduction took place in the western and southern sections, while intra-oceanic subduction occurred in the eastern section.Comprehensive analyses on U-Pb ages and Hf model ages of zircons, the main provenance of the Lengjiaxi Group is Cathaysia.
基金Supported by Wuling Mountaionus Area Study Center Opening Fund( WLYF-2012002)
文摘[Objective] The aim was to to enhance the technology to brew better the traditional grape wine, which provided theoretical basis for the development of Wuling Mountain grape wine. [Method] Taking grapes in Wuling Mountain as the row materials, Saccharomyces cerevisiae of different wines was chosen and brewed. The quality of grape wine was studied and the sensory evaluation was analyzed by dint of GC-MS. [Result] Result illustrated that the ethanol concentration of the wine brewed by exploration craft A was 68.5 g/L and that brewed by exploration craft B was 59.5 g/L; the higher alcohol content of craft A was 37.86% while that of craft B is 35.99%; craft A's percentage content of esters was 28.82% while that of B was 27.10%; craft A's percentage content of acids was 2.20% while that of B was 1.24%; the gamma-aminobutyric acid content of craft A was 33.2 g/100 ml while that of craft B was 35.4 g/100 ml. the essential amino-acid content of craft A was 11.8 g/100 ml while that of craft B was 13.9 g/100 ml .The result indicates that the wine brewed by craft A was aromatic and had special flavor. [Conclusion] In this case, the grape wine has particular flavor and has good property, which can be exploited.
文摘Fanjing Mountain in Tongren City,Guizhou Province.As the main summit of the Wuling Mountain Range,Fanjing stands at an elevation of 2,572 meters.It was one of the earliest landmasses in southern China to rise from the ocean and stillretains geological wonders dating back 1.4 billion years.
