[Objectives] To establish a method for determining the content of chlorogenic acid in Wuli Huichun Wan. [Methods] The content of chlorogenic acid in Wuli Huichun Wan was determined by high performance liquid chromatog...[Objectives] To establish a method for determining the content of chlorogenic acid in Wuli Huichun Wan. [Methods] The content of chlorogenic acid in Wuli Huichun Wan was determined by high performance liquid chromatography( HPLC) combined with Agilent Eclipse Plus C18 column( 4. 6 mm × 250 mm,5 μm),in mobile phase of 0. 4% phosphoric acid solution-acetonitrile( 12∶ 88) at flow rate of1. 0 m L/min,and detection wavelength of 327 nm. [Results] The linearity of chlorogenic acid in the range of 4-48 μg injection was good( r = 1); the average recovery rate was 99. 61% and the RSD was 0. 98%. [Conclusions] The established method is simple and accurate and has high reproducibility,thus can be used as quality control method for Wuli Huichun Wan.展开更多
Using the method of trophic state-composite index(TSI-CI)and the 12 months of monitoring data in 2010,we carry out initial exploration of the status of ecosystem health in Wuli Lake.First,we select four indicators,Chl...Using the method of trophic state-composite index(TSI-CI)and the 12 months of monitoring data in 2010,we carry out initial exploration of the status of ecosystem health in Wuli Lake.First,we select four indicators,Chla,SD,TP and TN,to conduct trophic state assessment using weighted index method;then after selecting physical,chemical and biological indicators to conduct nondimensionalization processing,we calculate the composite index and conduct comprehensive assessment.The results show that in 2010,the status of ecosystem health in Wuli Lake was the best in July,worst in August;when the composite trophic state indicators with Chla as the representative increase or decrease significantly and cross different nutritional grades,TSI will significantly deviate from CI,and the relationship between the two in the other time is not prominent.展开更多
文摘[Objectives] To establish a method for determining the content of chlorogenic acid in Wuli Huichun Wan. [Methods] The content of chlorogenic acid in Wuli Huichun Wan was determined by high performance liquid chromatography( HPLC) combined with Agilent Eclipse Plus C18 column( 4. 6 mm × 250 mm,5 μm),in mobile phase of 0. 4% phosphoric acid solution-acetonitrile( 12∶ 88) at flow rate of1. 0 m L/min,and detection wavelength of 327 nm. [Results] The linearity of chlorogenic acid in the range of 4-48 μg injection was good( r = 1); the average recovery rate was 99. 61% and the RSD was 0. 98%. [Conclusions] The established method is simple and accurate and has high reproducibility,thus can be used as quality control method for Wuli Huichun Wan.
基金Supported by Project of Wuxi Municipal Development and Reform Commission(2115019)
文摘Using the method of trophic state-composite index(TSI-CI)and the 12 months of monitoring data in 2010,we carry out initial exploration of the status of ecosystem health in Wuli Lake.First,we select four indicators,Chla,SD,TP and TN,to conduct trophic state assessment using weighted index method;then after selecting physical,chemical and biological indicators to conduct nondimensionalization processing,we calculate the composite index and conduct comprehensive assessment.The results show that in 2010,the status of ecosystem health in Wuli Lake was the best in July,worst in August;when the composite trophic state indicators with Chla as the representative increase or decrease significantly and cross different nutritional grades,TSI will significantly deviate from CI,and the relationship between the two in the other time is not prominent.
