Constructing electrode materials with large capacity and good conductivity is an effective approach to improve the capacitor performance of asymmetric supercapacitors(ASCs).In this paper,ZnCo_(2)S_(4)core-shell nanosp...Constructing electrode materials with large capacity and good conductivity is an effective approach to improve the capacitor performance of asymmetric supercapacitors(ASCs).In this paper,ZnCo_(2)S_(4)core-shell nanospheres are constructed by two-step hydrothermal method.In order to improve the chemical activity of ZnCo_(2)S_(4),ZnCo_(2)S_(4)is activated using cetyltrimethylammonium bromide(CTAB).Then,MXene nanosheets are fixed on the surface of ZnCo_(2)S_(4)by electrostatic selfassembly method to improve the specific surface area of ZnCo_(2)S_(4)and MXene-wrapped ZnCo_(2)S_(4)composite is prepared in this work.Owing to the synergy effect between MXene nanosheets and ZnCo_(2)S_(4)core-shell nanospheres,the as-prepared composite displays fast ion transfer rate and charge/discharge process.The capacity of the MXenewrapped ZnCo_(2)S_(4)composite can reach 1072 F·g^(-1),which is far larger than that of ZnCo_(2)S_(4)(407 F·g^(-1))at 1 A·g^(-1).An ASC device is assembled,which delivers 1.7 V potential window and superior cyclic stability(95.41%capacitance retention).Furthermore,energy density of this device is up to 30.46 Wh·kg^(-1)at a power density of850 W·kg^(-1).The above results demonstrate that MXenewrapped ZnCo_(2)S_(4)composite has great application prospects in electrochemical energy storage field.展开更多
Circular data as any other types of data are subjected to contamination with some unexpected observations which are known outliers. In this paper, four tests of discordancy for circular data based on M, C, D, and A st...Circular data as any other types of data are subjected to contamination with some unexpected observations which are known outliers. In this paper, four tests of discordancy for circular data based on M, C, D, and A statistics are extended to the wrapped Cauchy distribution to detect possible outliers. The cut-off points and the power of performances are investigated via extensive simulation study. Results show that tests perform better as the concentration of the samples is increased. Two real circular data sets are analysed for illustration.展开更多
In this paper we propose a new family of circular distributions, obtained by wrapping discrete skew Laplace distribution on Z = 0, ±1, ±2, around a unit circle. In contrast with many wrapped distributions, h...In this paper we propose a new family of circular distributions, obtained by wrapping discrete skew Laplace distribution on Z = 0, ±1, ±2, around a unit circle. In contrast with many wrapped distributions, here closed form expressions exist for the probability density function, the distribution function and the characteristic function. The properties of this new family of distribution are studied.展开更多
PMMA wrapped alumina siloxane gel was obtained by using poly (methyl methacrylate) (PMMA) to wrap alumina siloxane sol which was made from water glass, aluminum nitrate and α methacrylic acid. Meanwhile, rare eart...PMMA wrapped alumina siloxane gel was obtained by using poly (methyl methacrylate) (PMMA) to wrap alumina siloxane sol which was made from water glass, aluminum nitrate and α methacrylic acid. Meanwhile, rare earth ions were used to dope in the course of reaction, and stable suspensions was produced by putting PMMA wrapped microcapsule powder doped with rare earths into methy silicon oil. It is found that its electrorheological (ER) effect improves strikingly after loading electric field. At a certain concentration, the immediate viscosity increases from 95 to 178 Pa·s compared to the system without doping.展开更多
This letter proposes a new method for concurrent voiced speech separation. Firstly the Wrapped Discrete Fourier Transform (WDFT) is used to decompose the harmonic spectra of the mixed speeches. Then the individual spe...This letter proposes a new method for concurrent voiced speech separation. Firstly the Wrapped Discrete Fourier Transform (WDFT) is used to decompose the harmonic spectra of the mixed speeches. Then the individual speech is reconstructed by using the sinusoidal speech model. By taking advantage of the non-uniform frequency resolution of WDFT, harmonic spectra parameters can be estimated and separated accurately. Experimental results on mixed vowels separation show that the proposed method can recover the original speeches effectively.展开更多
Concrete cylindess wrapped with glass fabrics of various constructions were fabricated. Compressive behaviours of cylinders with and without fabric wrapping were investigated. Comparisons of the compressive characters...Concrete cylindess wrapped with glass fabrics of various constructions were fabricated. Compressive behaviours of cylinders with and without fabric wrapping were investigated. Comparisons of the compressive characters while using different fabrication parameters were made. It was demonstrated that the effect of the fabric reinforcent was obvious. The tensile strength of the filament used in the fabric played an important role as far as the anti-compression behaviour of the reinforced cylinders is concened.展开更多
Water pollution has become an urgent issue for our modem society, and it is highly desirable to rapidly deal with the water pollution without secondary pollution. In this paper, we have prepared a reduced graphene oxi...Water pollution has become an urgent issue for our modem society, and it is highly desirable to rapidly deal with the water pollution without secondary pollution. In this paper, we have prepared a reduced graphene oxide （RGO） wrapped sponge with superhydrophobicity and mechanically flexibility via a facile low-temperature thermal treatment method under a reducing atmosphere. The skeleton of this sponge is completely covered with RGO layers which are closely linked to the skeleton. This sponge has an abundant pore structure, high selectivity, good recyclability, low cost, and outstanding adsorption capacity for floating oil or heavy oil underwater. In addition, this sponge can maintain excellent adsorption performance for various oils and organic solvents over 50 cycles by squeezing, and exhibits extremely high separa- tion efficiencies, up to 6 × 10^6 and 3.6 × 10^6 L·m^-3.h^-1 in non-turbulent and turbulent water/oil systems, respec- tively. This superhydrophobic adsorbent with attractive properties may find various applications, especially in large-scale removal of organic contaminants and oil spill cleanup.展开更多
Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which lim...Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which limited their practical activity in Li–S reaction systems.In this study,we introduce a chemical vapor deposition(CVD)-triggered dry-chemistry approach for the preparation of graphene-cobalt(Co)based catalysts.The versatile CVD technique provides a dry and controllable reaction environment,effectively pledging the compact and clean catalytic interfaces between graphene and Co-based components.Additionally,programmed reactions introduce defects such as vacancies and nitrogen heteroatoms into the catalysts.Notably,the graphene layer number and Co valence state can be delicately manipulated by altering the CVD reaction temperature.Specifically,few-layer graphene wrapped Co/Co_(3)O_(4)(FGr-Co/Co_(3)O_(4))prepared at 450 ℃ shows higher catalytic activity than the multi-layer graphene wrapped Co/CoO(MGr-Co/CoO)synthesized at 550 ℃,attributed to its comprehensive control of clean interface,valence distribution range and defects.Leveraging these advantages,the battery with FGr-Co/Co_(3)O_(4)shows favorable working stability with a degradation rate of only 0.08%over 500 cycles at 1.0 C.Furthermore,under an elevated sulfur loading of 6.1 mg cm^(–2),the battery harvests a remarkable areal capacity of 5.9 mA h cm^(–2)along with stable cyclic operation.展开更多
Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong disability.Currently,there are no FDA-approved therapies a...Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong disability.Currently,there are no FDA-approved therapies available to promote nerve regeneration.Tacrolimus accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve surgery.The authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug exposure.Tacrolimus is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 days.Size and drug loading are adjustable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible byproducts.Tacrolimus released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic delivery.Given its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.展开更多
Composites derived from metal-organic frameworks(MOFs)show promise as catalysts for the photocat-alytic reduction of CO_(2).However,their potential is hindered by constraints such as limited light absorp-tion and slug...Composites derived from metal-organic frameworks(MOFs)show promise as catalysts for the photocat-alytic reduction of CO_(2).However,their potential is hindered by constraints such as limited light absorp-tion and sluggish electron transfer and separation,impacting the overall efficiency of the photocatalytic process.In this study,TiO_(2)nanocrystals,modified with Ptx+,underwent laser etching were encapsulated within the traditional MOF-ZIF-8 framework.This enhanced the adsorption capabilities for CO_(2)reactants and solar light,while also facilitating directed electron transfer and the separation of photogenerated charges.The finely-tuned catalyst demonstrates impressive CH_(4) selectivity at 9.5%,with yields of 250.24μmol g^(-1)h^(-1)for CO and 25.43μmol g^(-1)h^(-1)for CH_(4),utilizing water as a hole trap and H^(+)source.This study demonstrates the viability of achieving characteristics related to the separation of photogen-erated charges in TiO_(2)nanocrystals through laser etching and MOF composite catalysts.It offers novel perspectives for designing MOF-based catalysts with enhanced performance in artificial photosynthesis.展开更多
WRAP(Water Rights Analysis Package)水权分析模型是基于水资源优先分配制度来模拟和预测一个或多个流域内水资源使用及管理的模型。作者首次将WRAP水权分析模型应用于北京通州区,以1956～2007年通州区相关资料作为模型的计算数据,模...WRAP(Water Rights Analysis Package)水权分析模型是基于水资源优先分配制度来模拟和预测一个或多个流域内水资源使用及管理的模型。作者首次将WRAP水权分析模型应用于北京通州区,以1956～2007年通州区相关资料作为模型的计算数据,模拟与计算结果表明:通州地区的天然年径流总体趋势基本保持不变,调度径流呈微弱增长趋势,两者的年际变差均逐渐剧烈;历年的径流消耗量总体趋势亦基本不变,但回归流呈逐渐增长趋势;研究区内多年平均水量保证率为59.9%,工业用水、城镇生活用水和农村生活用水呈上升趋势,而农业用水量总体呈下降趋势。展开更多
Cu with and without La addition was prepared and the effect of a trace amount of La on the arc erosion behaviors and oxidation resistance of Cu alloys was investigated. The results indicate that CuLa alloy exhibits su...Cu with and without La addition was prepared and the effect of a trace amount of La on the arc erosion behaviors and oxidation resistance of Cu alloys was investigated. The results indicate that CuLa alloy exhibits superior oxidation resistance and arc erosion resistance. The contact resistance and temperature rise were obviously improved. The oxidation resistance of CuLa alloy mainly is due to the interface wrapping of La2O3 particles and CuLa alloy phase on Cu atoms. Thermodynamic calculation indicated that La2O3 could form preferentially in the CuLa alloy, which was beneficial for the protection of the Cu substrate. According to kinetics analysis, the activation energy of CuLa alloy was higher than that of pure Cu, indicating the better oxidation resistance of CuLa alloys.展开更多
目的:探讨抑制p53的反义转录本WRAP53α基因表达对顺铂(cisplatin,CDDP)诱导人骨肉瘤U-2OS细胞凋亡的影响。方法:不同浓度的CDDP处理U-2OS细胞后,MTT法检测细胞的增殖率;不同浓度CDDP处理36 h和10μmol/L CDDP处理不同时间以及si WRAP5...目的:探讨抑制p53的反义转录本WRAP53α基因表达对顺铂(cisplatin,CDDP)诱导人骨肉瘤U-2OS细胞凋亡的影响。方法:不同浓度的CDDP处理U-2OS细胞后,MTT法检测细胞的增殖率;不同浓度CDDP处理36 h和10μmol/L CDDP处理不同时间以及si WRAP53转染后,实时荧光定量PCR和蛋白质印迹法检测U-2OS细胞中WARAP53α和p53 m RNA及p53蛋白的表达水平;FCM法检测CDDP单独处理及si WRAP53转染后联合CDDP处理组U-2OS细胞的凋亡率。结果:CDDP处理后,U-2OS细胞的增殖率低于未处理的对照组(P<0.05),且呈浓度依赖性。CDDP处理后,U-2OS细胞中WRAP53αm RNA的表达水平明显高于未处理的空白对照组(P<0.05),且呈浓度和时间依赖性;p53 m RNA及p53蛋白的表达水平高于空白对照组(P<0.05)。si WRAP53转染后,WRAP53α和p53 m RNA及p53蛋白的表达水平低于空白对照组(P<0.05)。CDDP单独给药组U-2OS细胞凋亡率高于si WRAP53α转染联合CDDP处理组(P<0.05),2组细胞的凋亡率均高于空白对照组(P<0.05)。结论:CDDP可抑制U-2OS细胞的增殖,诱导WRAP53α和p53 m RNA及p53蛋白的表达;干扰WRAP53α基因的表达可下调p53的表达水平,并逆转CDDP诱导的细胞凋亡。展开更多
基金financially supported by the Fundamental Research Funds for the Central Universities (No. 2019XKQYMS16)
文摘Constructing electrode materials with large capacity and good conductivity is an effective approach to improve the capacitor performance of asymmetric supercapacitors(ASCs).In this paper,ZnCo_(2)S_(4)core-shell nanospheres are constructed by two-step hydrothermal method.In order to improve the chemical activity of ZnCo_(2)S_(4),ZnCo_(2)S_(4)is activated using cetyltrimethylammonium bromide(CTAB).Then,MXene nanosheets are fixed on the surface of ZnCo_(2)S_(4)by electrostatic selfassembly method to improve the specific surface area of ZnCo_(2)S_(4)and MXene-wrapped ZnCo_(2)S_(4)composite is prepared in this work.Owing to the synergy effect between MXene nanosheets and ZnCo_(2)S_(4)core-shell nanospheres,the as-prepared composite displays fast ion transfer rate and charge/discharge process.The capacity of the MXenewrapped ZnCo_(2)S_(4)composite can reach 1072 F·g^(-1),which is far larger than that of ZnCo_(2)S_(4)(407 F·g^(-1))at 1 A·g^(-1).An ASC device is assembled,which delivers 1.7 V potential window and superior cyclic stability(95.41%capacitance retention).Furthermore,energy density of this device is up to 30.46 Wh·kg^(-1)at a power density of850 W·kg^(-1).The above results demonstrate that MXenewrapped ZnCo_(2)S_(4)composite has great application prospects in electrochemical energy storage field.
文摘Circular data as any other types of data are subjected to contamination with some unexpected observations which are known outliers. In this paper, four tests of discordancy for circular data based on M, C, D, and A statistics are extended to the wrapped Cauchy distribution to detect possible outliers. The cut-off points and the power of performances are investigated via extensive simulation study. Results show that tests perform better as the concentration of the samples is increased. Two real circular data sets are analysed for illustration.
文摘In this paper we propose a new family of circular distributions, obtained by wrapping discrete skew Laplace distribution on Z = 0, ±1, ±2, around a unit circle. In contrast with many wrapped distributions, here closed form expressions exist for the probability density function, the distribution function and the characteristic function. The properties of this new family of distribution are studied.
文摘PMMA wrapped alumina siloxane gel was obtained by using poly (methyl methacrylate) (PMMA) to wrap alumina siloxane sol which was made from water glass, aluminum nitrate and α methacrylic acid. Meanwhile, rare earth ions were used to dope in the course of reaction, and stable suspensions was produced by putting PMMA wrapped microcapsule powder doped with rare earths into methy silicon oil. It is found that its electrorheological (ER) effect improves strikingly after loading electric field. At a certain concentration, the immediate viscosity increases from 95 to 178 Pa·s compared to the system without doping.
基金Supported by the National Natural Science Foundation of China (No.60172048).
文摘This letter proposes a new method for concurrent voiced speech separation. Firstly the Wrapped Discrete Fourier Transform (WDFT) is used to decompose the harmonic spectra of the mixed speeches. Then the individual speech is reconstructed by using the sinusoidal speech model. By taking advantage of the non-uniform frequency resolution of WDFT, harmonic spectra parameters can be estimated and separated accurately. Experimental results on mixed vowels separation show that the proposed method can recover the original speeches effectively.
基金This project is financed by the key laboratory of Advanced Composite Materials of Ministry of Education in Tianjin Polytechnicuniversity
文摘Concrete cylindess wrapped with glass fabrics of various constructions were fabricated. Compressive behaviours of cylinders with and without fabric wrapping were investigated. Comparisons of the compressive characters while using different fabrication parameters were made. It was demonstrated that the effect of the fabric reinforcent was obvious. The tensile strength of the filament used in the fabric played an important role as far as the anti-compression behaviour of the reinforced cylinders is concened.
文摘Water pollution has become an urgent issue for our modem society, and it is highly desirable to rapidly deal with the water pollution without secondary pollution. In this paper, we have prepared a reduced graphene oxide （RGO） wrapped sponge with superhydrophobicity and mechanically flexibility via a facile low-temperature thermal treatment method under a reducing atmosphere. The skeleton of this sponge is completely covered with RGO layers which are closely linked to the skeleton. This sponge has an abundant pore structure, high selectivity, good recyclability, low cost, and outstanding adsorption capacity for floating oil or heavy oil underwater. In addition, this sponge can maintain excellent adsorption performance for various oils and organic solvents over 50 cycles by squeezing, and exhibits extremely high separa- tion efficiencies, up to 6 × 10^6 and 3.6 × 10^6 L·m^-3.h^-1 in non-turbulent and turbulent water/oil systems, respec- tively. This superhydrophobic adsorbent with attractive properties may find various applications, especially in large-scale removal of organic contaminants and oil spill cleanup.
基金support of the National Natural Science Foundation of China(Grant No.52172239,52202038,and 52402247)the Innovative Funds Plan of Henan University of Technology(Grant No.2020ZKCJ07)the Natural Science Foundation of Shandong Province(Grant No.ZR2022QE081)。
文摘Graphene-metal based materials have been utilized in lithium-sulfur(Li–S)batteries owing to their integrated functionalities thus far.However,their synthesis has predominantly relied on wet-chemistry routes,which limited their practical activity in Li–S reaction systems.In this study,we introduce a chemical vapor deposition(CVD)-triggered dry-chemistry approach for the preparation of graphene-cobalt(Co)based catalysts.The versatile CVD technique provides a dry and controllable reaction environment,effectively pledging the compact and clean catalytic interfaces between graphene and Co-based components.Additionally,programmed reactions introduce defects such as vacancies and nitrogen heteroatoms into the catalysts.Notably,the graphene layer number and Co valence state can be delicately manipulated by altering the CVD reaction temperature.Specifically,few-layer graphene wrapped Co/Co_(3)O_(4)(FGr-Co/Co_(3)O_(4))prepared at 450 ℃ shows higher catalytic activity than the multi-layer graphene wrapped Co/CoO(MGr-Co/CoO)synthesized at 550 ℃,attributed to its comprehensive control of clean interface,valence distribution range and defects.Leveraging these advantages,the battery with FGr-Co/Co_(3)O_(4)shows favorable working stability with a degradation rate of only 0.08%over 500 cycles at 1.0 C.Furthermore,under an elevated sulfur loading of 6.1 mg cm^(–2),the battery harvests a remarkable areal capacity of 5.9 mA h cm^(–2)along with stable cyclic operation.
基金supported by the German Research Foundation(DA 2255/1-1to SCD)+4 种基金a SickKids Research Training Competition(RESTRACOMP)Graduate Scholarship(to KJWS)an Ontario Graduate Scholarship(to KJWS)a grant from Natural Sciences and Engineering Research Council of Canada(NSERC)(to KJWS)a Kickstarter grant from the Institute of Biomedical Engineering(BME)at the University of Toronto(to KJWS)the Abe Frank Fund from the Riley’s Children Foundation(GHB)。
文摘Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong disability.Currently,there are no FDA-approved therapies available to promote nerve regeneration.Tacrolimus accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve surgery.The authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug exposure.Tacrolimus is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 days.Size and drug loading are adjustable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible byproducts.Tacrolimus released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic delivery.Given its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.
文摘Composites derived from metal-organic frameworks(MOFs)show promise as catalysts for the photocat-alytic reduction of CO_(2).However,their potential is hindered by constraints such as limited light absorp-tion and sluggish electron transfer and separation,impacting the overall efficiency of the photocatalytic process.In this study,TiO_(2)nanocrystals,modified with Ptx+,underwent laser etching were encapsulated within the traditional MOF-ZIF-8 framework.This enhanced the adsorption capabilities for CO_(2)reactants and solar light,while also facilitating directed electron transfer and the separation of photogenerated charges.The finely-tuned catalyst demonstrates impressive CH_(4) selectivity at 9.5%,with yields of 250.24μmol g^(-1)h^(-1)for CO and 25.43μmol g^(-1)h^(-1)for CH_(4),utilizing water as a hole trap and H^(+)source.This study demonstrates the viability of achieving characteristics related to the separation of photogen-erated charges in TiO_(2)nanocrystals through laser etching and MOF composite catalysts.It offers novel perspectives for designing MOF-based catalysts with enhanced performance in artificial photosynthesis.
文摘WRAP(Water Rights Analysis Package)水权分析模型是基于水资源优先分配制度来模拟和预测一个或多个流域内水资源使用及管理的模型。作者首次将WRAP水权分析模型应用于北京通州区,以1956～2007年通州区相关资料作为模型的计算数据,模拟与计算结果表明:通州地区的天然年径流总体趋势基本保持不变,调度径流呈微弱增长趋势,两者的年际变差均逐渐剧烈;历年的径流消耗量总体趋势亦基本不变,但回归流呈逐渐增长趋势;研究区内多年平均水量保证率为59.9%,工业用水、城镇生活用水和农村生活用水呈上升趋势,而农业用水量总体呈下降趋势。
基金Project(51171146)supported by the National Natural Science Foundation of China
文摘Cu with and without La addition was prepared and the effect of a trace amount of La on the arc erosion behaviors and oxidation resistance of Cu alloys was investigated. The results indicate that CuLa alloy exhibits superior oxidation resistance and arc erosion resistance. The contact resistance and temperature rise were obviously improved. The oxidation resistance of CuLa alloy mainly is due to the interface wrapping of La2O3 particles and CuLa alloy phase on Cu atoms. Thermodynamic calculation indicated that La2O3 could form preferentially in the CuLa alloy, which was beneficial for the protection of the Cu substrate. According to kinetics analysis, the activation energy of CuLa alloy was higher than that of pure Cu, indicating the better oxidation resistance of CuLa alloys.
文摘目的:探讨抑制p53的反义转录本WRAP53α基因表达对顺铂(cisplatin,CDDP)诱导人骨肉瘤U-2OS细胞凋亡的影响。方法:不同浓度的CDDP处理U-2OS细胞后,MTT法检测细胞的增殖率;不同浓度CDDP处理36 h和10μmol/L CDDP处理不同时间以及si WRAP53转染后,实时荧光定量PCR和蛋白质印迹法检测U-2OS细胞中WARAP53α和p53 m RNA及p53蛋白的表达水平;FCM法检测CDDP单独处理及si WRAP53转染后联合CDDP处理组U-2OS细胞的凋亡率。结果:CDDP处理后,U-2OS细胞的增殖率低于未处理的对照组(P<0.05),且呈浓度依赖性。CDDP处理后,U-2OS细胞中WRAP53αm RNA的表达水平明显高于未处理的空白对照组(P<0.05),且呈浓度和时间依赖性;p53 m RNA及p53蛋白的表达水平高于空白对照组(P<0.05)。si WRAP53转染后,WRAP53α和p53 m RNA及p53蛋白的表达水平低于空白对照组(P<0.05)。CDDP单独给药组U-2OS细胞凋亡率高于si WRAP53α转染联合CDDP处理组(P<0.05),2组细胞的凋亡率均高于空白对照组(P<0.05)。结论:CDDP可抑制U-2OS细胞的增殖,诱导WRAP53α和p53 m RNA及p53蛋白的表达;干扰WRAP53α基因的表达可下调p53的表达水平,并逆转CDDP诱导的细胞凋亡。
