In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic...In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic Wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before optimization.Meanwhile, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.展开更多
Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide va...Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide variety of products. In this work, the antifungal activity of Wickerhamomyces anomalus against C. gloeosporioides isolated from contaminated avocados was evaluated. The antagonism and volatile compound inhibition were measured on Petri dishes. In the mixed cultures, the mycelia damage was observed by scanning electron microscope （SEM）. Chitinase and glucanase production by the antagonism was quantified by the reducing sugars method, and biofilm formation was evaluated with 1% crystal violet. The yeast W. anomalus could reduce the growth of C. gloeosporioides up to 65% by direct antagonism and 10% by volatile compounds. The antagonist did not allow the conidia germination and mycelia growth in any of the tested formulations. SEM showed mycelial damage caused by W. anomalus. The antagonist showed adhesion to the mycelium by a polysaccharide biofilm. The presence of mycelium stimulated the hydrolytic enzyme production with the maximal activity of 21.4 U/mg for chitinases at 24 h and 10 U/mg for glucanases at 60 h. These results showed that W. anomalus used together different mechanisms to express its antifungal activity against C. gloeosporioides. This study might be the first report for this phytopathogen isolated from avocado fruits, which could represent an opportunity to establish biocontrol of diseases for this agricultural product.展开更多
Wickerhamomyces anomalus makes a special contribution to the esters flavor of Chinese Baijiu.This study set out to investigate whether W.anomalus NCUF304.1 could improve the flavor of ethyl esters of Te-flavor Baijiu ...Wickerhamomyces anomalus makes a special contribution to the esters flavor of Chinese Baijiu.This study set out to investigate whether W.anomalus NCUF304.1 could improve the flavor of ethyl esters of Te-flavor Baijiu Jiupei(TFBJP).The days 10–30 of the Te-flavor Baijiu brewing stage was the key period for microorganisms to produce ethyl ester compounds.Among them,the content of ethyl acetate in the strengthening group of Jiupei with additional W.anomalus NCUF304.1 was significantly higher(P<0.05)than in the control group of Jiupei on days 10–20.Also,there was no significant difference in ethyl acetate content between the two samples until day 30 of fermentation.In addition,the contents of ethyl butyrate,ethyl lactate and ethyl hexanoate in TFBJP strengthened with W.anomalus NCUF304.1 were found to increase by 0.54,3.27 and 0.58 mg/kg,respectively.These findings evidenced the ability of W.anomalus NCUF304.1 in the Te-flavor Baijiu brewing for improving the flavor of ethyl esters.展开更多
Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phin...Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phingosine in plant seeds,relying on the extraction method is highly challenging.Currently,the primary method for obtaining phytosphingosine is the deacetylation of tetraacetyl phytosphingosine(TAPS)derived from fermentation.Wickerhamomyces ciferrii,an unconventional yeast from the pods of Dipteryx odorata,is the only known microorganism capable of naturally secreting TAPS,which is of great industrial value.In recent years,research and applications focused on modifying W.ciferrii for TAPS overproduction have increased rapidly.This review first describes the discovery history,applications,microbial synthesis pathway of TAPS.Research progress in using haploid breeding,mutagenesis breeding,and metabolic engineering to improve TAPS pro-duction is then summarized.In addition,the future prospects of TAPS production using the W.ciferrii platform are discussed in light of the current progress,challenges,and trends in this field.Finally,guidelines for future researches are also emphasized.展开更多
文摘In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic Wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before optimization.Meanwhile, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.
文摘Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide variety of products. In this work, the antifungal activity of Wickerhamomyces anomalus against C. gloeosporioides isolated from contaminated avocados was evaluated. The antagonism and volatile compound inhibition were measured on Petri dishes. In the mixed cultures, the mycelia damage was observed by scanning electron microscope （SEM）. Chitinase and glucanase production by the antagonism was quantified by the reducing sugars method, and biofilm formation was evaluated with 1% crystal violet. The yeast W. anomalus could reduce the growth of C. gloeosporioides up to 65% by direct antagonism and 10% by volatile compounds. The antagonist did not allow the conidia germination and mycelia growth in any of the tested formulations. SEM showed mycelial damage caused by W. anomalus. The antagonist showed adhesion to the mycelium by a polysaccharide biofilm. The presence of mycelium stimulated the hydrolytic enzyme production with the maximal activity of 21.4 U/mg for chitinases at 24 h and 10 U/mg for glucanases at 60 h. These results showed that W. anomalus used together different mechanisms to express its antifungal activity against C. gloeosporioides. This study might be the first report for this phytopathogen isolated from avocado fruits, which could represent an opportunity to establish biocontrol of diseases for this agricultural product.
基金National Natural Science Foundation of China(No.31960474)Research Project of State Key Laboratory of Food Science and Technology(SKLF-ZZB-202116)for supporting this workWe also thank Jiangxi Zhangshugong Wine and Spirits Co.,Ltd for its support and assistance of providing raw material and the brewing conditions of Teflavor Baijiu.
文摘Wickerhamomyces anomalus makes a special contribution to the esters flavor of Chinese Baijiu.This study set out to investigate whether W.anomalus NCUF304.1 could improve the flavor of ethyl esters of Te-flavor Baijiu Jiupei(TFBJP).The days 10–30 of the Te-flavor Baijiu brewing stage was the key period for microorganisms to produce ethyl ester compounds.Among them,the content of ethyl acetate in the strengthening group of Jiupei with additional W.anomalus NCUF304.1 was significantly higher(P<0.05)than in the control group of Jiupei on days 10–20.Also,there was no significant difference in ethyl acetate content between the two samples until day 30 of fermentation.In addition,the contents of ethyl butyrate,ethyl lactate and ethyl hexanoate in TFBJP strengthened with W.anomalus NCUF304.1 were found to increase by 0.54,3.27 and 0.58 mg/kg,respectively.These findings evidenced the ability of W.anomalus NCUF304.1 in the Te-flavor Baijiu brewing for improving the flavor of ethyl esters.
基金supported by the National Natural Science Foundation of China(22178173)the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20231119)+3 种基金the China Postdoctoral Science Foundation(2024M751420)the Jiangsu Agricultural Science and Technology Independent Innovation Fund Project(CX(22)3015)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTC2204)the Newton Advanced Fellowships(NAF\R1\201187).
文摘Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phingosine in plant seeds,relying on the extraction method is highly challenging.Currently,the primary method for obtaining phytosphingosine is the deacetylation of tetraacetyl phytosphingosine(TAPS)derived from fermentation.Wickerhamomyces ciferrii,an unconventional yeast from the pods of Dipteryx odorata,is the only known microorganism capable of naturally secreting TAPS,which is of great industrial value.In recent years,research and applications focused on modifying W.ciferrii for TAPS overproduction have increased rapidly.This review first describes the discovery history,applications,microbial synthesis pathway of TAPS.Research progress in using haploid breeding,mutagenesis breeding,and metabolic engineering to improve TAPS pro-duction is then summarized.In addition,the future prospects of TAPS production using the W.ciferrii platform are discussed in light of the current progress,challenges,and trends in this field.Finally,guidelines for future researches are also emphasized.
