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高精度生物检测技术的系统化整合:温控扩展、荧光灵敏度提升与定量标准化研究进展 被引量:1
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作者 杨坤鹏 赵晖 +4 位作者 王雷 王哲 蔡承伦 吕佩昀 王宝 《吉林医学》 2025年第6期1466-1469,共4页
生物检测技术是现代生物医学研究和临床诊断的基础,其精度和可靠性直接影响研究结果的科学性和诊断结果的准确性。随着精准医疗和个体化治疗的快速发展,对生物检测技术的精度要求日益提高。高精度生物检测技术的系统化整合不仅需要优化... 生物检测技术是现代生物医学研究和临床诊断的基础,其精度和可靠性直接影响研究结果的科学性和诊断结果的准确性。随着精准医疗和个体化治疗的快速发展,对生物检测技术的精度要求日益提高。高精度生物检测技术的系统化整合不仅需要优化各项技术的关键参数,还需要建立统一的标准化体系,以确保不同实验室、不同设备之间结果的可比性和一致性。 展开更多
关键词 PCR 流式细胞仪 WESTERN印迹 生物检测技术 系统化整合
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自体黄韧带干预TGF-β1/Smad3信号通路抑制兔硬膜外纤维瘢痕形成的实验研究
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作者 王鹏 张德宝 +5 位作者 张杨 张海斌 时嘉 张少杰 李树文 吴一民 《中国临床解剖学杂志》 北大核心 2025年第1期69-75,共7页
目的通过对不同组别的兔分别行椎板切除术,对比其各组术后3、6周TGF-β1、Smad3蛋白表达量以及两者相应的mRNA表达水平,从而探讨自体黄韧带抑制腰椎术后硬膜外纤维瘢痕形成与TGF-β1/Smad3信号通路的关系。方法将于内蒙古医科大学实验... 目的通过对不同组别的兔分别行椎板切除术,对比其各组术后3、6周TGF-β1、Smad3蛋白表达量以及两者相应的mRNA表达水平,从而探讨自体黄韧带抑制腰椎术后硬膜外纤维瘢痕形成与TGF-β1/Smad3信号通路的关系。方法将于内蒙古医科大学实验动物中心购买的48只6~8月龄的日本大白兔,分为保留黄韧带组,自体脂肪回置组,不保留黄韧带组,对不同组别进行手术造模。术后对每组间的实验动物分别饲养3、6周后进行处死(3、6周各处死8只)。采用Western blot蛋白定量分析、RTPCR技术测定各组样本中的TGF-β1、Smad3蛋白含量及mRNA表达水平,并将以上数据分别进行组间比较,分析其差异性。结果①Western blot检测结果:3、6周保留组TGF-β1、Smad3蛋白表达量小于不保留组和脂肪组(P<0.05),不保留组和脂肪组之间并未发现明显差异。②RT-PCR结果显示:对于TGF-β1、Smad3两者的mRNA表达量而言,3、6周保留组明显小于不保留组和脂肪组(P<0.05),不保留组和脂肪组之间并未见明显差异。结论腰椎手术术后,会形成不同程度的硬膜外纤维瘢痕。若在术中保留自体黄韧带,可减少硬膜外成纤维细胞的形成,其机制与TGF-β1/Smad3信号通路有关。 展开更多
关键词 黄韧带 纤维瘢痕 TGF-β1/Smad3信号通路 Western blot RT-PCR技术
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条件性胰岛β细胞HLF基因敲除小鼠构建与鉴定
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作者 侯梦龙 齐心语 +4 位作者 吴建凤 廖启超 马杰 周磊 李一星 《安徽医科大学学报》 北大核心 2025年第8期1432-1439,共8页
目的为了探究肝白血病因子(HLF)在糖尿病中的作用机制,构建条件性胰岛β细胞HLF基因敲除小鼠动物模型。方法在细胞水平上,通过CCK-8试验验证HLF抑制或过表达对MIN6细胞增殖的影响。通过RT-qPCR和Western blot,分别在mRNA水平和蛋白水平... 目的为了探究肝白血病因子(HLF)在糖尿病中的作用机制,构建条件性胰岛β细胞HLF基因敲除小鼠动物模型。方法在细胞水平上,通过CCK-8试验验证HLF抑制或过表达对MIN6细胞增殖的影响。通过RT-qPCR和Western blot,分别在mRNA水平和蛋白水平检测HLF抑制或过表达效果。将HLF flox/flox转基因小鼠与Pdx1-Cre+/-小鼠(C57BL/6J)杂交繁育,获得子代小鼠。利用PCR方法鉴定小鼠基因型,通过RT-qPCR技术和Western blot检测HLF基因在胰岛β细胞敲除小鼠(HLF flox/flox Cre+/-)和对照小鼠(HLF flox/flox)中的mRNA水平和蛋白水平表达差异,验证敲除效果。同时取2组小鼠的胰岛组织制作石蜡切片并进行苏木精-伊红(HE)染色分析。结果HLF基因抑制或过表达对MIN6细胞增殖无显著影响。在MIN6细胞中抑制HLF基因,其mRNA表达水平较对照组下降了74%,蛋白表达水平较对照组下降了60%;过表达HLF基因后,其mRNA表达水平为对照组的2.13倍,蛋白表达水平为对照组的1.8倍。敲除小鼠的HLF基因mRNA表达水平较对照组下降了89%,蛋白表达水平较对照组下降了65%。HE染色结果显示:敲除小鼠胰岛组织内细胞形态与对照小鼠无明显差异。抑制HLF使MIN6细胞中糖原含量提高约20%。结论成功构建HLF基因敲除小鼠,为研究HLF在糖尿病发病机制中的作用提供了动物模型。 展开更多
关键词 肝白血病因子 条件性胰岛β细胞 RT-QPCR Western blot 基因敲除 C57BL/6J小鼠
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CSF1R^(+/-)小鼠的构建和基因鉴定
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作者 周园园 刘崇 +4 位作者 王安琪 张慧茹 邱佳琪 朱梦娟 涂佳杰 《安徽医科大学学报》 北大核心 2025年第5期884-889,共6页
目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外... 目的构建集落刺激因子1受体杂合(CSF1R^(+/-))小鼠并分析其基因型,为疾病病理机制及药物靶点提供动物模型基础。方法根据Cre/Loxp系统设计一种线性化的靶向载体,在集落刺激因子1受体(CSF1R)基因第5外显子上游插入一个Loxp位点,在第5外显子下游插入一个双侧有Loxp位点的新霉素抗性盒(PGK-neo)。将线性化的靶向载体电穿孔至胚胎干细胞(ES)。将正确靶向的ES注射到C57BL/6J小鼠的胚泡中得到嵌合小鼠,与透明带3-Cre(Zp3-Cre)小鼠进行繁殖。新生小鼠出生后9~14天编号并剪鼠尾,提取小鼠的DNA,通过聚合酶链式反应(PCR)和琼脂糖凝胶电泳鉴定小鼠基因型,筛选出CSF1R^(+/-)小鼠。应用流式细胞术检测小鼠巨噬细胞中CSF1R的表达;Western blot检测小鼠组织中CSF1R的蛋白表达。结果琼脂糖凝胶电泳结果显示,野生小鼠(WT)扩增出453 bp的条带,CSF1R^(+/-)小鼠扩增出453 bp和650 bp的条带。流式细胞术结果显示,与WT组比较,CSF1R^(+/-)组小鼠的腹腔来源巨噬细胞(PM)和骨髓来源巨噬细胞(BMDM)中的CSF1R低表达(P<0.05)。Western blot结果显示,与WT组比较,CSF1R^(+/-)组小鼠脾脏、肾脏、脑组织中的CSF1R蛋白低表达(P<0.05)。结论成功构建、繁育和鉴定CSF1R^(+/-)小鼠,为进一步揭示CSF1R在免疫调节中的潜在机制研究提供动物模型基础。 展开更多
关键词 CSF1R PCR Western blot 流式细胞术 Cre/Loxp 动物模型
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Guidelines for the diagnosis and treatment of depressive disorders by integrating Chinese and Western medicine(English edition) 被引量:3
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作者 Lanying Liu Jianjun Wang +16 位作者 Wei Li Jing Gao Wangtao Li Yan Li Liyuan Luo Liyuan Guo Yiying Hu Yongjun Chen Hongyan Chen Lin Yu Bin Fen Hongxiao Jia Zhangjin Zhang Zhaojun Yan Wei Chen Zhangsheng Yu Zhen Wang 《General Psychiatry》 2025年第1期1-15,共15页
INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depres... INTRODUCTION.Depressive disorders are mental illnesses that seriously affect public health.There are approximately 320 million patients with depression worldwide,accounting for 4.4% of the total disease burden.1Depression leads to social and occupational impairment,diminished quality of life and an elevated risk of death by suicide. 展开更多
关键词 DIAGNOSIS mental illnesses depressive disorders chinese medicine western medicine TREATMENT
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猪衣原体ompA基因的原核表达与纯化及其蛋白功能预测
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作者 李宁倩 禹思宇 +6 位作者 李桐菲 李文超 魏颖 谭建锡 胡忆文 唐连飞 刘伟 《湖南畜牧兽医》 2025年第5期29-33,共5页
为了将猪衣原体ompA基因作为目标抗原,为后续建立间接ELISA检测方法提供理论依据,开展了该基因的原核表达与纯化工作,并利用生物信息学方法对ompA蛋白的理化性质、亲疏水性、跨膜区域和抗原表位等进行了分析与预测。结果显示:该研究成... 为了将猪衣原体ompA基因作为目标抗原,为后续建立间接ELISA检测方法提供理论依据,开展了该基因的原核表达与纯化工作,并利用生物信息学方法对ompA蛋白的理化性质、亲疏水性、跨膜区域和抗原表位等进行了分析与预测。结果显示:该研究成功构建了重组质粒,并通过SDS-PAGE和Western blot确认该蛋白以包涵体形式表达,分子量约为27 kDa;该蛋白的预测分子质量为23.31921 kDa,理论等电点为4.37,不含跨膜区,属于亲水性蛋白,含有7个抗原决定簇,平均抗原倾向值为1.0316。结果表明:ompA蛋白具备良好的免疫反应性,是免疫反应中的关键成分,适合作为间接ELISA的包被抗原。该研究为深入理解猪衣原体的致病机制及开发相应的防控技术提供了理论基础。 展开更多
关键词 猪衣原体ompA基因 重组质粒 表达及纯化 Western blot验证 生物信息学预测 间接ELISA
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基于网络药理学和实验验证探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制
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作者 杨子烨 谭征 +4 位作者 刘洁 刘润花 杨畅 关晶 张建民 《中南药学》 2025年第8期2165-2172,共8页
目的运用网络药理学、分子对接以及体外细胞实验探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制。方法检索TCMSP、BATMAN-TCM、PubChem、SwissTargetPrediction等数据库,获得杏贝止咳祛痰口服液活性成分、相关作用靶点以及儿童支气... 目的运用网络药理学、分子对接以及体外细胞实验探究杏贝止咳祛痰口服液治疗儿童支气管炎的作用机制。方法检索TCMSP、BATMAN-TCM、PubChem、SwissTargetPrediction等数据库,获得杏贝止咳祛痰口服液活性成分、相关作用靶点以及儿童支气管炎相应疾病靶点,并将杏贝止咳祛痰口服液作用靶点与疾病靶点的交集作为潜在作用靶点;利用Cytoscape软件构建成分-疾病网络及蛋白互作网络,分析其网络拓扑结构,筛选关键成分及核心靶点,并进行分子对接验证;采用R语言对交集靶点进行GO功能及KEGG富集分析。最后,基于香烟烟雾提取物(CSE)诱导人正常支气管上皮细胞16-HBE构建体外细胞模型,采用Western blot技术进行相关通路靶点的验证。结果杏贝止咳祛痰口服液关键成分包括光甘草定、槲皮素、山柰酚等,主要通过调控TNF-α、STAT3、EGFR、PTGS2等在内的核心靶点介导的AGE-RAGE等信号通路发挥治疗儿童支气管炎的作用。Western blot实验结果显示,与空白组相比,模型组TNF-α、STAT3、MAPK3、ICAM1和PIK3CD蛋白的表达显著上调(P<0.01);与模型组相比,杏贝止咳祛痰口服液给药组对应蛋白显著下调(P<0.01)。结论杏贝止咳祛痰口服液可通过多成分、多靶点、多途径改善儿童支气管炎临床症状,其作用机制可能与抑制AGE-RAGE信号传导通路的活化有关。 展开更多
关键词 儿童支气管炎 杏贝止咳祛痰口服液 网络药理学 Western blot AGE-RAGE信号通路
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Efficacy and safety of Tripterygium wilfordii glycosides tablets combined with Western medicine for patients with rheumatic immune diseases
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作者 Hang Shu Xiao-Yu Chen +2 位作者 Jie Zhao Pin Li Zhen Sun 《World Journal of Clinical Cases》 SCIE 2025年第6期14-23,共10页
BACKGROUND Rheumatic immune diseases are a group of chronic inflammatory diseases charac-terized by joint and systemic multi-organ involvement,including rheumatoid arthritis,systemic lupus erythematosus,and Sjogren’s... BACKGROUND Rheumatic immune diseases are a group of chronic inflammatory diseases charac-terized by joint and systemic multi-organ involvement,including rheumatoid arthritis,systemic lupus erythematosus,and Sjogren’s syndrome,among others.The pathogenesis of these diseases is related to the abnormal activation and regulatory imbalance of the immune system.The prevalence and morbidity of rheumatic immune diseases are high,imposing a significant burden on patients'quality of life and socio-economic costs.Currently,the treatment of rheumatic immune diseases mainly relies on Western medicine,such as non-steroidal anti-inflammatory drugs,glucocorticoids,disease-modifying antirheumatic drugs,and biologics.However,the therapeutic effects of Western medicine are not ideal,some patients poorly respond or are resistant to Western medicine,and long-term use often causes various adverse reactions.AIM To systematically evaluate the efficacy and safety of Tripterygium wilfordii gly-cosides tablets combined with Western medicine in the treatment of patients with rheumatic immune diseases.METHODS This study conducted a meta-analysis to systematically evaluate the efficacy and safety of Tripterygium wilfordii glycosides tablets combined with Western medicine for patients with rheumatic immune diseases.Chinese and English databases were searched for randomized controlled trials(RCTs)on the treatment of rheumatic immune diseases with Tripterygium wilfordii glycosides tablets combined with Western medicine.The quality of the included studies was assessed using the Cochrane risk of bias assessment tool.Meta-analysis was performed using RevMan 5.4 software.RESULTS The meta-analysis included 11 RCTs involving 1026 patients with rheumatic immune diseases.The combined treatment significantly reduced the risk of disease recurrence(relative risk=1.07,95%confidence interval:1.01-1.15,P<0.05)and showed no significant heterogeneity(I2=0%,P=0.53),indicating that Tripterygium wilfordii glycosides tablets combined with Western medicine is an effective method to reduce the possibility of postoperative recurrence in patients with rheumatic immune diseases.However,due to the limited number and quality of the studies included,these results should be interpreted with caution.CONCLUSION Tripterygium wilfordii glycosides tablets combined with Western medicine is an effective and safe treatment option for patients with rheumatic immune diseases and can be considered a clinical choice.However,more high-quality research is needed to validate this conclusion and provide more solid evidence for clinical practice. 展开更多
关键词 Rheumatic immune diseases Tripterygium wilfordii polyglycosides tablets Western medicine treatment Systematic review META-ANALYSIS
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Burden of mental disorders and risk factors in the Western Pacific region from 1990 to 2021
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作者 Ya-Xin Xu Xiao-Xuan Niu +8 位作者 Wen-Chang Jia Jing Wen Xue-Lin Cheng Yan Han Ming-Hui Peng Jing Zhou Yao Liu Sun-Fang Jiang Xiao-Pan Li 《World Journal of Psychiatry》 SCIE 2025年第1期93-105,共13页
BACKGROUND The burden of mental disorders(MD)in the Western Pacific Region(WPR)re-mains a critical public health concern,with substantial variations across demogra-phics and countries.AIM To analyze the burden of MD i... BACKGROUND The burden of mental disorders(MD)in the Western Pacific Region(WPR)re-mains a critical public health concern,with substantial variations across demogra-phics and countries.AIM To analyze the burden of MD in the WPR from 1990 to 2021,along with associated risk factors,to reveal changing trends and emerging challenges.METHODS We used data from the Global Burden of Disease 2021,analyzing prevalence,incidence,and disability-adjusted life years(DALYs)of MD from 1990 to 2021.Statistical methods included age-standardisation and uncertainty analysis to address variations in population structure and data completeness.RESULTS Between 1990 and 2021,the prevalence of MD rose from 174.40 million cases[95%uncertainty interval(UI):160.17-189.84]to 234.90 million cases(95%UI:219.04-252.50),with corresponding DALYs increasing from 22.8 million(95%UI:17.22-28.79)to 32.07 million(95%UI:24.50-40.68).During this period,the burden of MD shifted towards older age groups.Depressive and anxiety disorders were predominant,with females showing higher DALYs for depressive and anxiety disorders,and males more affected by conduct disorders,attention-deficit hyperactivity disorder,and autism spectrum disorders.Australia,New Zealand,and Malaysia reported the highest burdens,whereas Vietnam,China,and Brunei Darussalam reported the lowest.Additionally,childhood sexual abuse and bullying,and intimate partner violence emerged as significant risk factors.CONCLUSION This study highlights the significant burden of MD in the WPR,with variations by age,gender,and nation.The coronavirus disease 2019 pandemic has exacerbated the situation,emphasizing the need for a coordinated response. 展开更多
关键词 Mental disorders Western pacific region Global Burden of Disease Risk factors Disability-adjusted life years
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Studies on the antioxidant properties of Staphylococcus epidermidis fermentation broth
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作者 Wenlin Geng Ming Li Yuhua Cao 《日用化学工业(中英文)》 北大核心 2025年第10期1275-1283,共9页
In this work, Staphylococcus epidermidis (S. epidermidis) was used to prepare the fermentation broths with antioxidant activity. Through the optimization of the carbon source, three kinds of S. epidermidis fermentatio... In this work, Staphylococcus epidermidis (S. epidermidis) was used to prepare the fermentation broths with antioxidant activity. Through the optimization of the carbon source, three kinds of S. epidermidis fermentation broth were obtained and designated as SFB, Gly-SFB, and Glu-SFB, which were cultivated in beef protein medium and the beef protein medium supplemented with glycerol or glucose, respectively. The differences in antioxidant efficacy of SFB, Gly-SFB and Glu-SFB were investigated by evaluating intracellular ROS fluorescence intensity, SOD enzyme activity and MDA concentration. Gly-SFB and Glu-SFB exhibited a greater capacity to eliminate ROS as compared to that of SFB. The intracellular SOD enzyme activity increased as the concentrations of SFB and Gly-SFB increased. Nevertheless, the intracellular SOD enzyme activity was the highest after the treatment with Glu-SFB at the low concentrations. The intracellular MDA content reached a lower value after the treatment with Gly-SFB and Glu-SFB at lower concentrations, which was opposite to the case after the treatment with SFB. WB indicated that the S. epidermidis fermentation broth regulated the expression of relevant proteins in the Nrf2-Keap1 signaling pathway to exhibit the antioxidant effects. This indicates that the S. epidermidis fermentation broth promotes the expression of relevant proteins in the Nrf2-Keap1 signaling pathway, consequently, antioxidant benefits were exerted. The fermentation broth that were prepared by incorporating glycerol or glucose into the culture medium can augment their antioxidant activity. 展开更多
关键词 S.epidermidis fermentation broth ANTIOXIDANT Western Blotting
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Cohort study on the treatment of BRAF V600E mutant metastatic colorectal cancer with integrated Chinese and western medicine
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作者 Jiang-Yu Bian Yu-Fang Feng +1 位作者 Wen-Ting He Tong Zhang 《World Journal of Clinical Oncology》 2025年第1期25-33,共9页
BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor prognosis.In the previous cohor... BACKGROUND Patients with BRAF V600E mutant metastatic colorectal cancer(mCRC)have a low incidence rate,poor biological activity,suboptimal response to conventional treatments,and a poor prognosis.In the previous cohort study on mCRC conducted by our team,it was observed that integrated Chinese and Western medicine treatment could significantly prolong the overall survival(OS)of patients with colorectal cancer.Therefore,we further explored the survival benefits in the population with BRAF V600E mutant mCRC.AIM To evaluate the efficacy of integrated Chinese and Western medicine in the treatment of BRAF V600E mutant metastatic colorectal cancer.METHODS A cohort study was conducted on patients with BRAF V600E mutant metastatic colorectal cancer admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences and Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region from January 2016 to December 2022.The patients were divided into two cohorts.RESULTS A total of 34 cases were included,with 23 in Chinese-Western medicine cohort(cohort A)and 11 in Western medicine cohort(cohort B).The median overall survival was 19.9 months in cohort A and 14.2 months in cohort B,with a statistically significant difference(P=0.038,hazard ratio=0.46).The 1-3-year survival rates were 95.65%(22/23),39.13%(9/23),and 26.09%(6/23)in cohort A,and 63.64%(7/11),18.18%(2/11),and 9.09%(1/11)in cohort B,respectively.Subgroup analysis showed statistically significant differences in median OS between the two cohorts in the right colon,liver metastasis,chemotherapy,and first-line treatment subgroups(P<0.05).CONCLUSION Integrated Chinese and Western medicine can prolong the survival and reduce the risk of death in patients with BRAF V600E mutant metastatic colorectal cancer,with more pronounced benefits observed in patients with right colon involvement,liver metastasis,combined chemotherapy,and first-line treatment. 展开更多
关键词 Metastatic colorectal cancer BRAF V600E mutation Integrated Chinese and Western medicine Cohort study
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ENSO-induced Latitudinal Variation of the Subtropical Jet Modulates Extreme Winter Precipitation over the Western Himalaya
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作者 Priya BHARATI Pranab DEB +2 位作者 Kieran M.R.HUNT Andrew ORR Mihir Kumar DASH 《Advances in Atmospheric Sciences》 2025年第3期427-437,共11页
In this study,we investigate the complex relationship between western disturbances(WDs),the El Ni?o–Southern Oscillation(ENSO),and extreme precipitation events(EPEs) in the western Himalaya(WH) during the extended wi... In this study,we investigate the complex relationship between western disturbances(WDs),the El Ni?o–Southern Oscillation(ENSO),and extreme precipitation events(EPEs) in the western Himalaya(WH) during the extended winter season(November–March).WDs west of WH coincide with 97% of recorded EPEs,contributing substantially(32% in winter,11% annually) to total precipitation within WH.WDs are 6% less frequent and 4% more intense during El Ni?o than La Ni?a to the west of WH.During El Ni?o(compared to La Ni?a) years,WDs co-occurring with EPEs are significantly more intense and associated with 17% higher moisture transport over “WH box”(the selected region where most of the winter precipitation over WH occurs).This results in twice the EPE frequency during El Ni?o periods than La Ni?a periods.A substantial southward shift(~180 km) of the subtropical jet(STJ) axis during El Ni?o brings WD tracks further south towards their primary moisture sources,especially the Arabian Sea.We have shown that WDs that are both more intense and pass to the south of their typical latitudes have higher levels of vertically integrated moisture flux(VIMF)within them.VIMF convergence in the most intense pentile of WDs is 5.7 times higher than in the weakest,and is 3.4 times higher in the second lowest latitude pentile than in the highest.Overall,this study demonstrates a direct link between changes in the latitudinal position and intensity of WDs associated with the winter STJ,and moisture convergence,which leads to the occurrence of EPEs over WH during ENSO phases. 展开更多
关键词 western Himalaya extreme precipitation western disturbances ENSO moisture flux WINTER
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Retraction:miR-641 functions as a tumor suppressor by targeting MDM2 in human lung cancer
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作者 Oncology Research Editorial Office 《Oncology Research》 2025年第3期737-737,共1页
Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in man... Following the publication,concerns have been raised about a number of figures in this article.The western blots in this article were presented with atypical,unusually shaped and possibly anomalous protein bands in many cases. 展开更多
关键词 western blots protein bands protein band human lung cancer tumor suppressor MDM MIR western blot
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Inaugural Editorial
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作者 ZHANG Xiaoxiao 《World Journal of Integrated Traditional and Western Medicine》 2025年第3期I0004-I0005,共2页
Dear colleagues and esteemed explorers of medicine across the globe,As the newly appointed Editor-in-Chief of the World Journal of Integrated traditional and western Medicine(WJIM),I stand deeply conscious of the prof... Dear colleagues and esteemed explorers of medicine across the globe,As the newly appointed Editor-in-Chief of the World Journal of Integrated traditional and western Medicine(WJIM),I stand deeply conscious of the profound mission this journal embodies.Since its inception in 2015,WJIM has held the distinguished status of being the only English-language academic publication exclusively hosted by the China Association of Chinese Medicine.Dedicated to the harmonious integration of traditional Chinese and western medical systems,the journal aspires to bridge the gap between ancient healing wisdom and contemporary scientific knowledge.With an ambitious vision,WJIM strives to rank among the world’s top-tier journals and to reshape the global narrative and discourse surrounding traditional medicine.At this pivotal juncture,we stand ready to embark on a new journey:to build a high-level academic platform fostering in-depth dialogue between traditional wisdom and modern science,where the convergence of Eastern and Western medicine will spark the light of innovation in human health technology. 展开更多
关键词 academic publication ancient healing wisdom integrated traditional western medicine integrated traditional western medicine wjim i contemporary scientific knowledge harmonious integration World Journal Integrated Traditional Western Medicine China Association Chinese Medicine
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Encounter of Chinese Medicine and Modern Western Medicine in China
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作者 Michael Shiyung LIU JIANG Yuhong 《Chinese Medicine and Culture》 2025年第3期225-227,F0002,共4页
1 Introduction The history of medicine in modern China has frequently been framed through a lens of“Westernization”,in which traditional Chinese medicine(TCM)is portrayed as grad-ually yielding to modern Western med... 1 Introduction The history of medicine in modern China has frequently been framed through a lens of“Westernization”,in which traditional Chinese medicine(TCM)is portrayed as grad-ually yielding to modern Western medicine.Such a binary framework,however,oversimplifies the intricate realities of medical encounters in China. 展开更多
关键词 modern western medicine Chinese medicine traditional chinese medicine tcm WESTERNIZATION traditional Chinese medicine modern China medical encounters history medicine
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Instruction for authors
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《World Journal of Integrated Traditional and Western Medicine》 2025年第1期I0004-I0005,共2页
World Journal of Integrated traditional and western Medicine(WJIM)is a journal published quarterly in English supervised by China Association for Science and Technology,and sponsored by China Association of Chinese Me... World Journal of Integrated traditional and western Medicine(WJIM)is a journal published quarterly in English supervised by China Association for Science and Technology,and sponsored by China Association of Chinese Medicine.WJIM aims to report the experience and achievements of Chinese medicine and Integrative medicine in clinical,scientific research,etc.,to explore the ideas and methods of the future development of Chinese and western integrative medicine,to introduce the latest progress at home and abroad in the related fields and to promote the international exchanges of Chinese medicine and integrative medicine. 展开更多
关键词 Integrative medicine chinese medicine integrative medicine Chinese medicine Western medicine Clinical research Scientific research integrated traditional western medicine wjim International exchanges
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Wildfires’ lasting impact on river quality
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作者 宋鹭 《疯狂英语(新悦读)》 2025年第10期9-11,76,共4页
Years after wildfires burn forests and watersheds(分水岭)in the Western US,pollutants left behind continue to poison rivers and streams,and have existed for a longer time than scientists previously estimated.
关键词 POLLUTANTS river quality western US watersheds WILDFIRES
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Morphology and phylogeny of two species of Bathypathes from the tropical western Pacific,with description of a new species
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作者 Ting LÜ Zifeng ZHAN Kuidong XU 《Journal of Oceanology and Limnology》 2025年第1期248-260,共13页
The black coral genus Bathypathes Brook,1889 represents a relatively diverse and ecologically important assemblage comprising the deep-sea hard substrate communities.Recent phylogenetic investigations have revealed th... The black coral genus Bathypathes Brook,1889 represents a relatively diverse and ecologically important assemblage comprising the deep-sea hard substrate communities.Recent phylogenetic investigations have revealed the polyphyletic nature of Bathypathes,urging further morphological analysis to understand its systematics.By combing morphological and molecular data,we describe Bathypathes multifurcata sp.nov.and report B.pseudoalternata Molodtsova,Opresko&Wagner,2022based on the specimens collected from the tropical western Pacific.The phylogenetic topology reconstructed using nuclear ITS1-5.8S-ITS2 and three mitochondrial fragments suggests the separation of Bathypathes into four groups.Our analysis of morphological characters,particularly the colony branch and pinnule patterns also roughly classify Bathypathes into four morphological groups A–D.Group A is characterized by the unbranched colony and the subopposite arrangement of pinnules.In contrast to Group A,Groups B–D have the alternately arranged pinnules.Among these,Group C exhibits a distinctly long unpinnulated stem compared to Groups B and D.Group B has unbranched colony,relatively short unpinnulated stem,and spines on pinnules have blunt apex.Within Group D,large colonies(higher than 40 cm)have one or a few branches,small colonies(less than 40 cm)are unbranched,and spines on pinnules have acute apex.Further data particularly those species without sequences are necessary to verify the classification of these groups. 展开更多
关键词 Antipatharia black coral TAXONOMY PHYLOGENY SEAMOUNT western Pacific
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A new species of genus Thalassometra AH Clark,1907(Crinoidea:Tropiometroidea:Thalassometridae)from a seamount on the Caroline Ridge in the northwest Pacific
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作者 Shao’e SUN Zijie MEI Zhongli SHA 《Journal of Oceanology and Limnology》 2025年第4期1313-1319,共7页
During the expedition to an unnamed seamount of Caroline Ridge in the northwest Pacific Ocean,one Thalassometra specimen was sampled,and careful morphological assessment suggested that this specimen represents a new s... During the expedition to an unnamed seamount of Caroline Ridge in the northwest Pacific Ocean,one Thalassometra specimen was sampled,and careful morphological assessment suggested that this specimen represents a new species within the genus Thalassometra:Thalassometra brachia sp.nov.This new species was collected at a depth of 688 m and is characterized by the dome-like centrodorsal,large number of arms(43 in total),irregularly arranged sockets with three per radial area,P 2 and P 3,similar in size and character and smaller than P 1,spinous median carination on the first and second ossicles of the division series and on the first two brachials,and dense spicules in brachial bases. 展开更多
关键词 new species CRINOIDEA Thalassometra SEAMOUNT western Pacific
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