Wedelolide is a coumarin-like active substance extracted from Ecliptae Herba.It not only has pharmacological effects of anti-pulmonary fibrosis,anti-arthritis,blood vessel protection and liver protection,but also effe...Wedelolide is a coumarin-like active substance extracted from Ecliptae Herba.It not only has pharmacological effects of anti-pulmonary fibrosis,anti-arthritis,blood vessel protection and liver protection,but also effectively inhibits the proliferation of cancer cells and induces apoptosis of cancer cells,thereby delaying the further development of malignant tumors.In this paper,the pharmacological effects and mechanisms of wedelactone were reviewed to lay a foundation for further study and clinical application of wedelactone.展开更多
Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, ...Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, potentiodynamic polarization and impedance methods and the extracts of Eclipta alba were found to be effective corrosion pickling inhibitor. The effect of immersion time and temperature revealed that the extracts of Eclipta alba with an optimum concentration of 8.0% v/v showed maximum inhibition efficiency of 99.6% at 3 h immersion time and 30℃. Arrhenius plots for mild steel immersed in 1N HCl solution in the absence and presence of optimum concentration （8.0% in v/v） of Eclipta alba extract showed the effect of temperature. Polarization studies indicate that this plant extract acts as a mixed type inhibitor. The adsorption of Eclipta alba follows Langmuir adsorption isotherm. The inhibition action may be due to the presence of the Wedelactone and also the alkaloid Ecliptine present in the leaves of Eclipta alba.展开更多
Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further...Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage polarization.Methods:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue staining.Inflammatory factors were assessed using the ELISA kits.The expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence assay.The protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot analysis.Results:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and cells.WED not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 expression.WED also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of IκB.Besides,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflammatory by the regulation of M1/M2 macrophage polarization via the PI3K/AKT/NF-κB signaling pathway.展开更多
基金Supported by the Talent Training Program for the Reform and Development of Local Colleges and Universities of the Central Government(2020GSP16)Innovation and Entrepreneurship Training Planning Project for University Students in Heilongjiang Province(202310223173).
文摘Wedelolide is a coumarin-like active substance extracted from Ecliptae Herba.It not only has pharmacological effects of anti-pulmonary fibrosis,anti-arthritis,blood vessel protection and liver protection,but also effectively inhibits the proliferation of cancer cells and induces apoptosis of cancer cells,thereby delaying the further development of malignant tumors.In this paper,the pharmacological effects and mechanisms of wedelactone were reviewed to lay a foundation for further study and clinical application of wedelactone.
文摘Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, potentiodynamic polarization and impedance methods and the extracts of Eclipta alba were found to be effective corrosion pickling inhibitor. The effect of immersion time and temperature revealed that the extracts of Eclipta alba with an optimum concentration of 8.0% v/v showed maximum inhibition efficiency of 99.6% at 3 h immersion time and 30℃. Arrhenius plots for mild steel immersed in 1N HCl solution in the absence and presence of optimum concentration （8.0% in v/v） of Eclipta alba extract showed the effect of temperature. Polarization studies indicate that this plant extract acts as a mixed type inhibitor. The adsorption of Eclipta alba follows Langmuir adsorption isotherm. The inhibition action may be due to the presence of the Wedelactone and also the alkaloid Ecliptine present in the leaves of Eclipta alba.
基金supported by the National Natural Science Foundation of China(No.81774190,81903832).
文摘Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage polarization.Methods:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue staining.Inflammatory factors were assessed using the ELISA kits.The expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence assay.The protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot analysis.Results:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and cells.WED not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 expression.WED also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of IκB.Besides,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflammatory by the regulation of M1/M2 macrophage polarization via the PI3K/AKT/NF-κB signaling pathway.
