Wantong Jingu Tablet(WJT),a mixture of traditional Chinese medicine,was reported to relieve the symptoms of rheumatoid arthritis(RA),but its pharmacological mechanism was not completely understood.The aim of this stud...Wantong Jingu Tablet(WJT),a mixture of traditional Chinese medicine,was reported to relieve the symptoms of rheumatoid arthritis(RA),but its pharmacological mechanism was not completely understood.The aim of this study was to investigate the therapeutic mechanisms of WJT for RA in vivo.The effects of WJT on joint pathology,as well as the levels of Bax,Bcl-2,caspase-3,cleaved-caspase-3,ERK1/2,pERK1/2,TNF-α,IL-1β,and IL-6 were measured using collagen-induced arthritis(CIA)rats.The intestinal flora composition and the metabolites alteration were analyzed by 16S rDNA sequencing and metabolomics method,respectively.We found that WJT ameliorated the severity of the CIA rats which might be mediated by inducing apoptosis,inactivating the MEK/ERK signals and reducing the production of pro-inflammatory cytokines.WJT,in part,relieved the gut microbiota dysbiosis,especially bacterial phylum Bacteroidetes,Tenericutes and Deferribacteres,as well as bacterial genus Vibrio,Macrococcus and Vagococcus.3’-N-debenzoyl-2’-deoxytaxol,tubulysin B,and magnoline were significantly associated with the specific genera.We identified serotonin,glutathione disulfide,N-acetylneuraminic acid,naphthalene and thromboxane B2 as targeted molecules via metabolomics.Our findings contributed to the understanding of RA pathogenesis,and WJT played essential roles in gut microbiota health and metabolite modulation in the CIA rats.展开更多
通过使用单独的无机阴离子柱,在测量F,Cl,NO3^-,SO4^2-,HPO4^-等无机阴离子的同时测量柠檬酸根。使用Metrosep A Supp 5-250/4.0无机色谱柱,10%的Na2CO3淋洗液,2%的H2SO4抑制剂。泵速为0.5ml/min。测量结果峰形分离度较高,所有测量离子...通过使用单独的无机阴离子柱,在测量F,Cl,NO3^-,SO4^2-,HPO4^-等无机阴离子的同时测量柠檬酸根。使用Metrosep A Supp 5-250/4.0无机色谱柱,10%的Na2CO3淋洗液,2%的H2SO4抑制剂。泵速为0.5ml/min。测量结果峰形分离度较高,所有测量离子标准曲线R2都在0.99以上,其中Cl以及柠檬酸根的R2达到0.999以上。使用以柠檬酸根为碳源的反硝化细菌培养液作为实际水样经行测量,并同常规检测方法进行对比,其结果精确。该方法操作简便,重复性好,灵敏度高,所得结果精确。展开更多
文摘Wantong Jingu Tablet(WJT),a mixture of traditional Chinese medicine,was reported to relieve the symptoms of rheumatoid arthritis(RA),but its pharmacological mechanism was not completely understood.The aim of this study was to investigate the therapeutic mechanisms of WJT for RA in vivo.The effects of WJT on joint pathology,as well as the levels of Bax,Bcl-2,caspase-3,cleaved-caspase-3,ERK1/2,pERK1/2,TNF-α,IL-1β,and IL-6 were measured using collagen-induced arthritis(CIA)rats.The intestinal flora composition and the metabolites alteration were analyzed by 16S rDNA sequencing and metabolomics method,respectively.We found that WJT ameliorated the severity of the CIA rats which might be mediated by inducing apoptosis,inactivating the MEK/ERK signals and reducing the production of pro-inflammatory cytokines.WJT,in part,relieved the gut microbiota dysbiosis,especially bacterial phylum Bacteroidetes,Tenericutes and Deferribacteres,as well as bacterial genus Vibrio,Macrococcus and Vagococcus.3’-N-debenzoyl-2’-deoxytaxol,tubulysin B,and magnoline were significantly associated with the specific genera.We identified serotonin,glutathione disulfide,N-acetylneuraminic acid,naphthalene and thromboxane B2 as targeted molecules via metabolomics.Our findings contributed to the understanding of RA pathogenesis,and WJT played essential roles in gut microbiota health and metabolite modulation in the CIA rats.
文摘通过使用单独的无机阴离子柱,在测量F,Cl,NO3^-,SO4^2-,HPO4^-等无机阴离子的同时测量柠檬酸根。使用Metrosep A Supp 5-250/4.0无机色谱柱,10%的Na2CO3淋洗液,2%的H2SO4抑制剂。泵速为0.5ml/min。测量结果峰形分离度较高,所有测量离子标准曲线R2都在0.99以上,其中Cl以及柠檬酸根的R2达到0.999以上。使用以柠檬酸根为碳源的反硝化细菌培养液作为实际水样经行测量,并同常规检测方法进行对比,其结果精确。该方法操作简便,重复性好,灵敏度高,所得结果精确。
