In plants,WUSCHEL-related homeobox1(WOX1)homologs promote lamina mediolateral outgrowth.However,the downstream components linking WOX1 and lamina development remain unclear.In this study,we revealed the roles of WOX1 ...In plants,WUSCHEL-related homeobox1(WOX1)homologs promote lamina mediolateral outgrowth.However,the downstream components linking WOX1 and lamina development remain unclear.In this study,we revealed the roles of WOX1 in palmate leaf expansion in cucumber(Cucumis sativus).A cucumber mango fruit(mf)mutant,resulting from truncation of a WOX1-type protein(CsWOX1),displayed abnormal lamina growth and defects in the development of secondary and smaller veins.CsWOX1 was expressed in the middle mesophyll and leaf margins and rescued defects of the Arabidopsis wox1 prs double mutant.Transcriptomic analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf development.Analysis of the cucumber mf rl(round leaf)double mutant revealed that CsWOX1 functioned in vein development via PINOID(CsPID1)-controlled auxin transport.Overexpression of CsWOX1 in cucumber(CsWOX1-OE)affected vein patterning and produced‘butterfly-shaped’leaves.CsWOX1 physically interacted with CsTCP4a,which may account for the abnormal lamina development in the mf mutant line and the smaller leaves in the CsWOX1-OE plants.Our findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport;moreover,CsWOX1 regulates leaf size by controlling CIN-TCP genes.展开更多
The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence (NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary structure and advanced struc...The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence (NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary structure and advanced structure of WUS protein inRaphanus sativusare analyzed by using bioinformatics methods. The results show that the cDNA was 1298 bp, with no intron, contains an open reading frame of 936bp, encoding 312aa protein. The protein coded byRaphanus sativusgene showed 74% similarity toArabidopsis thaliana.展开更多
日前，在内华达州拉斯维加斯举行的2004微软管理技术峰会(MMS)上，微软公布了它的下一代软件更新服务(SUS)2．0服务器应用程序，公司已将其改名为Windows更新服务(Windows Update Services，WUS)。WUS是一系列针对Windows Server 2003...日前，在内华达州拉斯维加斯举行的2004微软管理技术峰会(MMS)上，微软公布了它的下一代软件更新服务(SUS)2．0服务器应用程序，公司已将其改名为Windows更新服务(Windows Update Services，WUS)。WUS是一系列针对Windows Server 2003的更新(微软现在称之为“特性包”)中最新推出的。对中小企业来说，本次发布将可能是公司对Windows Server 2003最重要的更新之一。展开更多
Since July last year,Wang Zixian,a first-year visual communications graduate student at Wuhan University of Science and Technology(WUST),has posted her hand-drawn works on an online platform in order to share her expe...Since July last year,Wang Zixian,a first-year visual communications graduate student at Wuhan University of Science and Technology(WUST),has posted her hand-drawn works on an online platform in order to share her experience of taking postgraduate exams,and has attracted 12,000 fans and generated 65,000 thumbs-up.展开更多
基金supported by the National Key Research and Development Program of China(2019YFD1000300)the National Natural Science Foundation of China(Nos.31672150 and 31872111).
文摘In plants,WUSCHEL-related homeobox1(WOX1)homologs promote lamina mediolateral outgrowth.However,the downstream components linking WOX1 and lamina development remain unclear.In this study,we revealed the roles of WOX1 in palmate leaf expansion in cucumber(Cucumis sativus).A cucumber mango fruit(mf)mutant,resulting from truncation of a WOX1-type protein(CsWOX1),displayed abnormal lamina growth and defects in the development of secondary and smaller veins.CsWOX1 was expressed in the middle mesophyll and leaf margins and rescued defects of the Arabidopsis wox1 prs double mutant.Transcriptomic analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf development.Analysis of the cucumber mf rl(round leaf)double mutant revealed that CsWOX1 functioned in vein development via PINOID(CsPID1)-controlled auxin transport.Overexpression of CsWOX1 in cucumber(CsWOX1-OE)affected vein patterning and produced‘butterfly-shaped’leaves.CsWOX1 physically interacted with CsTCP4a,which may account for the abnormal lamina development in the mf mutant line and the smaller leaves in the CsWOX1-OE plants.Our findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport;moreover,CsWOX1 regulates leaf size by controlling CIN-TCP genes.
文摘The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence (NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary structure and advanced structure of WUS protein inRaphanus sativusare analyzed by using bioinformatics methods. The results show that the cDNA was 1298 bp, with no intron, contains an open reading frame of 936bp, encoding 312aa protein. The protein coded byRaphanus sativusgene showed 74% similarity toArabidopsis thaliana.
文摘日前，在内华达州拉斯维加斯举行的2004微软管理技术峰会(MMS)上，微软公布了它的下一代软件更新服务(SUS)2．0服务器应用程序，公司已将其改名为Windows更新服务(Windows Update Services，WUS)。WUS是一系列针对Windows Server 2003的更新(微软现在称之为“特性包”)中最新推出的。对中小企业来说，本次发布将可能是公司对Windows Server 2003最重要的更新之一。
文摘Since July last year,Wang Zixian,a first-year visual communications graduate student at Wuhan University of Science and Technology(WUST),has posted her hand-drawn works on an online platform in order to share her experience of taking postgraduate exams,and has attracted 12,000 fans and generated 65,000 thumbs-up.
