Plant oils are increasingly sought after as sustainable sources of bioenergy for biodiesel production and high-value biochemicals.Although oilseed crops currently serve as the primary source of plant oils,meeting the ...Plant oils are increasingly sought after as sustainable sources of bioenergy for biodiesel production and high-value biochemicals.Although oilseed crops currently serve as the primary source of plant oils,meeting the rising global demand on limited arable land,without compromising food security,remains a major challenge.Therefore,metabolic engineering of high-biomass bioenergy feedstocks has been widely explored to enhance the conversion of carbon stored in vegetative tissues into energy-dense triacylglycerol(TAG).Significant progress has been made in boosting TAG accumulation in the vegetative tissues of various plant species through bioengineering strategies.These efforts span from single-gene modifications to the coordinated expression of key lipogenic factors such as WRI1,DGAT1/2,and OLE1.The resulting fatty acid and TAG profiles,however,often vary depending on the targeted plant species and promoter(s)used.This review summarizes the roles of essential lipogenic factors in plant oil biosynthesis and highlights recent advances in metabolic engineering across diverse crop species through combinatorial expression of these factors.We also discuss future strategies for achieving high-level oil production without incurring growth penalties.By offering new perspectives on metabolic engineering,this work aims to support the development of plants as efficient biofuel feedstocks,contributing to the global effort to address energy challenges.展开更多
In this article, the biosynthetic pathways of storage oil accumulation in oilseed plants were briefly introduced, and the transcription factors, such as B3 do- main supeffamily genes, lecl gene, wril gene etc., and th...In this article, the biosynthetic pathways of storage oil accumulation in oilseed plants were briefly introduced, and the transcription factors, such as B3 do- main supeffamily genes, lecl gene, wril gene etc., and their important role in oil accumulation regulation was mainly elucidated. Overexpession of transcription factors as feasible ways of genetic manipulation to increase oJl content in oilseed crops are promising in a long-term perspective.展开更多
SUMO PROTEASE RELATED TO FERTILITY 1(SPF1)and SPF2 are responsible for deSUMOylation of SUMO-conjugated protein substrates and for maintaining protein SUMOylation homeostasis.To date,the role of SUMO proteases in fatt...SUMO PROTEASE RELATED TO FERTILITY 1(SPF1)and SPF2 are responsible for deSUMOylation of SUMO-conjugated protein substrates and for maintaining protein SUMOylation homeostasis.To date,the role of SUMO proteases in fatty acid biosynthesis and lipid accumulation remains unclear.Here,we demonstrate that the Arabidopsis thaliana mutants spf1-1,spf2-1,and spf1-1 spf2-1 exhibit increased seed size and elevated seed oil content(SOC).We further show that SPF1 and SPF2 interact with WRINKLED1(WRI1),a master regulator of the transcriptional control of plant oil synthesis.Genetic analyses indicate that the spf1-1 wri1-3 and spf2-1 wri1-3 double mutants,as well as the spf1-1 spf2-1 wri1-3 triple mutant,phenocopy wri1-3 and display severe seed shriveling,indicating that SPF1 and SPF2 act upstream of WRI1.WRI1 was identified as a SUMO1 substrate with two conserved SUMOylation sites,lysine 257(K257)and K266,in cruciferous plants,with K257 acting as the dominant site required for seed oil synthesis.SUMOylation enhances WRI1 stability,whereas SPF1and SPF2mediated deSUMOylation promotes WRI1 degradation.In spf1-1,spf2-1,and spf1-1 spf2-1 mutants,the abundance of SUMOylated WRI1 increases during seed development and correlates with elevated seed oil accumulation.Together,these results indicate that SPF1 and SPF2 negatively regulate oil synthesis by deSUMOylating WRI1,establishing a dynamic SUMOylation and deSUMOylation switch centered on the SPF1/SPF2-WRI1 module that fine-tunes seed development and oil synthesis.展开更多
基金the Natural Science Foundation of China(32300231)to Jiang Wangthe Natural Science Foundation of Hainan Province(324MS023)to Jiang Wang.
文摘Plant oils are increasingly sought after as sustainable sources of bioenergy for biodiesel production and high-value biochemicals.Although oilseed crops currently serve as the primary source of plant oils,meeting the rising global demand on limited arable land,without compromising food security,remains a major challenge.Therefore,metabolic engineering of high-biomass bioenergy feedstocks has been widely explored to enhance the conversion of carbon stored in vegetative tissues into energy-dense triacylglycerol(TAG).Significant progress has been made in boosting TAG accumulation in the vegetative tissues of various plant species through bioengineering strategies.These efforts span from single-gene modifications to the coordinated expression of key lipogenic factors such as WRI1,DGAT1/2,and OLE1.The resulting fatty acid and TAG profiles,however,often vary depending on the targeted plant species and promoter(s)used.This review summarizes the roles of essential lipogenic factors in plant oil biosynthesis and highlights recent advances in metabolic engineering across diverse crop species through combinatorial expression of these factors.We also discuss future strategies for achieving high-level oil production without incurring growth penalties.By offering new perspectives on metabolic engineering,this work aims to support the development of plants as efficient biofuel feedstocks,contributing to the global effort to address energy challenges.
基金Supported by Science and Technology Foundation of Guizhou Province [(2011)2089]Engineering Technology Research Center Building Fund of Guizhou Province ([2012]4006)Excellent Scientific and Educational Governor Fund of Guizhou Province ([2009]06)~~
文摘In this article, the biosynthetic pathways of storage oil accumulation in oilseed plants were briefly introduced, and the transcription factors, such as B3 do- main supeffamily genes, lecl gene, wril gene etc., and their important role in oil accumulation regulation was mainly elucidated. Overexpession of transcription factors as feasible ways of genetic manipulation to increase oJl content in oilseed crops are promising in a long-term perspective.
基金supported by grants from the National Key Research and Development Plan of China(2021YFF1000103)the National Natural Science Foundation of China(32470339 and 32072095)+1 种基金the Ministry of Education(MOE)of Singapore Tier 2 Program(MOE-T2EP30123-0001 to W.M.)the Asian Institute of Modern Agronomy(AIMA)R&D Funding Program(AIMA-2024-WP2 to W.M.)。
文摘SUMO PROTEASE RELATED TO FERTILITY 1(SPF1)and SPF2 are responsible for deSUMOylation of SUMO-conjugated protein substrates and for maintaining protein SUMOylation homeostasis.To date,the role of SUMO proteases in fatty acid biosynthesis and lipid accumulation remains unclear.Here,we demonstrate that the Arabidopsis thaliana mutants spf1-1,spf2-1,and spf1-1 spf2-1 exhibit increased seed size and elevated seed oil content(SOC).We further show that SPF1 and SPF2 interact with WRINKLED1(WRI1),a master regulator of the transcriptional control of plant oil synthesis.Genetic analyses indicate that the spf1-1 wri1-3 and spf2-1 wri1-3 double mutants,as well as the spf1-1 spf2-1 wri1-3 triple mutant,phenocopy wri1-3 and display severe seed shriveling,indicating that SPF1 and SPF2 act upstream of WRI1.WRI1 was identified as a SUMO1 substrate with two conserved SUMOylation sites,lysine 257(K257)and K266,in cruciferous plants,with K257 acting as the dominant site required for seed oil synthesis.SUMOylation enhances WRI1 stability,whereas SPF1and SPF2mediated deSUMOylation promotes WRI1 degradation.In spf1-1,spf2-1,and spf1-1 spf2-1 mutants,the abundance of SUMOylated WRI1 increases during seed development and correlates with elevated seed oil accumulation.Together,these results indicate that SPF1 and SPF2 negatively regulate oil synthesis by deSUMOylating WRI1,establishing a dynamic SUMOylation and deSUMOylation switch centered on the SPF1/SPF2-WRI1 module that fine-tunes seed development and oil synthesis.
