[目的]蓖麻(Ricinus communis L.)是一种重要的工业油料作物。为了鉴定蓖麻WRI1(WRINKLED1)转录因子,搞清蓖麻种子油脂生物合成调控机制。[方法]利用生物信息学工具全基因组鉴定蓖麻RcWRI1蛋白及基因,分析蓖麻RcWRI1蛋白结构特征和功能...[目的]蓖麻(Ricinus communis L.)是一种重要的工业油料作物。为了鉴定蓖麻WRI1(WRINKLED1)转录因子,搞清蓖麻种子油脂生物合成调控机制。[方法]利用生物信息学工具全基因组鉴定蓖麻RcWRI1蛋白及基因,分析蓖麻RcWRI1蛋白结构特征和功能;应用qPCR检测蓖麻RcWRI1基因表达谱。[结果]鉴定获得2种蓖麻RcWRI1蛋白,即RcWRI1-1(446AA)和RcWRI1-2(443AA),均为蓖麻RcWRI1基因(LOC8283400)编码的产物。与拟南芥AtWRI1相似,RcWRI1-1和RcWRI1-2都具有2个高度保守AP2结构域,属不稳定的亲水蛋白,预测其具有调控脂肪酸代谢等功能。RcWRI1-1和RcWRI1-2在蓖麻花、叶、种子和果皮均表达,RcWRI1-1的表达量明显高于RcWRI1-2,且在种子中表达量最高。[结论]蓖麻RcWRI1基因编码两种RcWRI1蛋白(RcWRI1-1和RcWRI1-2),其中RcWRI1-1是主要表达者,在蓖麻叶、花、种子和果皮油脂合成中起重要调控作用。本研究可为种子油脂生物合成调控机制提供理论基础。展开更多
Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related ...Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.展开更多
文摘[目的]蓖麻(Ricinus communis L.)是一种重要的工业油料作物。为了鉴定蓖麻WRI1(WRINKLED1)转录因子,搞清蓖麻种子油脂生物合成调控机制。[方法]利用生物信息学工具全基因组鉴定蓖麻RcWRI1蛋白及基因,分析蓖麻RcWRI1蛋白结构特征和功能;应用qPCR检测蓖麻RcWRI1基因表达谱。[结果]鉴定获得2种蓖麻RcWRI1蛋白,即RcWRI1-1(446AA)和RcWRI1-2(443AA),均为蓖麻RcWRI1基因(LOC8283400)编码的产物。与拟南芥AtWRI1相似,RcWRI1-1和RcWRI1-2都具有2个高度保守AP2结构域,属不稳定的亲水蛋白,预测其具有调控脂肪酸代谢等功能。RcWRI1-1和RcWRI1-2在蓖麻花、叶、种子和果皮均表达,RcWRI1-1的表达量明显高于RcWRI1-2,且在种子中表达量最高。[结论]蓖麻RcWRI1基因编码两种RcWRI1蛋白(RcWRI1-1和RcWRI1-2),其中RcWRI1-1是主要表达者,在蓖麻叶、花、种子和果皮油脂合成中起重要调控作用。本研究可为种子油脂生物合成调控机制提供理论基础。
文摘Among APETALA2 (AP2)-type plant specific transcription factor family, WRINKLED1 (WRI1), has appeared to be a master gene transcriptionally regulating a set of carbon metabolism- and fatty acid synthesis (FAS)-related genes responsible for seed specific triacylglycerols (TAGs) storage in oil plants. B3 type transcription factors, such as ABI3 and FUS3, are known to be involved in seed development, such as seed storage protein synthesis and maturation. Based on the recent whole genome sequence data of castor bean (Ricinus communis L.), putative WRI1 homologs (RcWRI1, RcWRI2) specifically expressed in castor bean seed have been identified by comparing organ specific expression profiles among seed development-related transcription factors, seed storage specific genes (Ricin, RcOleosin) and a set of FAS genes including genes for sucrose synthase (RcSUS2), biotin carboxyl carrier protein (a subunit of acetyl-CoA carboxylase, RcBCCP2) and ketoacyl-acyl carrier protein synthase (RcKAS1). Immunoreactive signals with WRI1, FUS3 and ABI5-related polypeptides were also detected in seed specifically, consistent with the expression profiles of seed development-related genes. The WRI1 binding consensus sites, [CnTnG](n)(7)[CG], designated as the AW-box, were found at the promoter region of RcBCCP2 and RcKAS1. Thus, RcWRI1 possibly play a pivotal role in seed specific TAGs storage during seed development by directly activating FAS -related genes.
